item_id in_vivo
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item2 "Neo-resistant recombinant ESCs were analyzed for correct 3' and 5' targeting through hybridization by standard protocols, and we identified seven correctly targeted clones. Following ESC cell injection, several high-percentage chimera males were born. These were bred with Cre-expressing mice, and Cre-mediated excision of Alkbh5 exon 1 was tested in 26 agouti F1 pups. Two animals tested positive for the excised allele. These two mice, heterozygous for the constitutive allele (Alkbh5+/-), were analyzed by Southern blot analysis and used for further breeding to generate Alkbh5-/- mice."
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item10 "Eight adult female C57BL/6 mice from the same pool of litters were housed at 22 ± 1 ℃ with 12-h light cycle and were mated with their male siblings. The female mice were fed with a low-fat diet supplemented with betaine from mating and throughout gestation and lactation. Litters were culled to five males per dam. Thirty offspring weaned at the age of day 21 and then were randomly assigned to three groups fed with either a low-fat diet (LF), high-fat diet (HF), or high-fat diet supplemented with betaine (HB) for 6 weeks."
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item12 "HCT116, Y2KD-116 and con-116 cells were resuspended at 1 × 106 cells per 50 ul of PBS. Cells were injected into the exteriorized spleen after abdominal incision."
item13 "A total of 1,000 breast cancer cells were injected into the mammary fat pad of 6-8-wk-old female NSG mice in a 1:1 suspension of Matrigel (BD Biosciences) in PBS solution. At 10 wk after injection, mice were examined for the presence of tumors, which were harvested for analysis."
item14 "1,000 MDA-MB-231 subclone cells were injected into the mammary fat pad of randomly chosen 6-to-8 week-old female severe combined immunodeficiency (SCID) mice in a 1:1 suspension of Matrigel (BD Biosciences) in PBS (n = 7 mice per subclone)."
item15 "Male athymic BALB/c nude mice (5 weeks old) were used. Subcutaneous tumor growth assays, liver metastasis model, and tail vein injection model were performed as described.Metastases were detected using the IVIS@Lumina II system (Caliper Life Sciences, Hopkinton, MA) 10 minutes after intraperitoneal injection of 4.0 mg luciferin (Gold Biotech) in 50 uL of saline."
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item20 "Sixteen 10-wk-old male C57BL/6 J mice [wild-type (WT) mice] and six 10-wk-old male obese C57BL/6Job/ob (OB) mice were purchased from Nanjing Biomedical Research Institute of Nanjing University. WT mice were randomly divided into 2 groups (8 mice/group): normal chow diet (ND) and high-fat diet (HFD) groups. The ND group was fed with a normal chow diet containing10% fat, 20% protein and 70% carbohydrate. The HFD group was fed a HFD containing 45% fat, 20% protein and 35% carbohydrate. OB mice were fed with ND. All mice were housed at 22 ± 1 ℃ under a 12-h light cycle with free access to water and diet during the experiment."
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item23 "For the animal survival analysis, mice were intracranially injected with 10,000 GSCs and maintained until moribund or 80 days after injection. For the rescue studies, GSCs with ALKBH5 or FOXM1-AS shRNAs were co-transfected with a FOXM1, ALKBH5 wild-type or mutant expression construct."
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item26 "Mixture of Cas9 mRNA (20 ng/uL) and two sgRNAs (5 ng/uL each) were injected into the cytoplasm and male pronucleus of the zygote, obtained by CBF1 mating."
item27 "All mice described above were maintained on the C57BL/6J (B6) background. Mettl3- and Mettl14-floxed mice (Mettl3flox/flox and Mettl14flox/flox) were then bred with germ cell-specific expressed Cre mice including Vasa-Cre mouse line (Jackson Laboratory, Bar Harbor, Maine, USA) and Stra8-GFPCre mouse line for excising the loxP-flanked exon 4 and exon 2 to generate germ cell-specific Mettl3 and Mettl14KO mice, respectively. Germ cell-specific Mettl3 and Mettl14 double KO mice were obtained by crossing Mettl3flox/floxMettl14flox/flox with Mettl3flox/+Mettl14flox/+ or Mettl3flox/+Mettl14flox/flox carried germ cell-specific expressed Cre mice."
item28 "All mice described above were maintained on the C57BL/6J (B6) background. Mettl3- and Mettl14-floxed mice (Mettl3flox/flox and Mettl14flox/flox) were then bred with germ cell-specific expressed Cre mice including Vasa-Cre mouse line (Jackson Laboratory, Bar Harbor, Maine, USA) and Stra8-GFPCre mouse line for excising the loxP-flanked exon 4 and exon 2 to generate germ cell-specific Mettl3 and Mettl14KO mice, respectively. Germ cell-specific Mettl3 and Mettl14 double KO mice were obtained by crossing Mettl3flox/floxMettl14flox/flox with Mettl3flox/+Mettl14flox/+ or Mettl3flox/+Mettl14flox/flox carried germ cell-specific expressed Cre mice."
item29 "500,000 selected cells were injected via tail vein or retro-orbital route into female NSG (6-8 week old) recipient mice that had been sublethally irradiated with 475 cGy one day before transplantation."
item30 "500,000 selected cells were injected via tail vein or retro-orbital route into female NSG (6-8 week old) recipient mice that had been sublethally irradiated with 475 cGy one day before transplantation."
item31 "500,000 selected cells were injected via tail vein or retro-orbital route into female NSG (6-8 week old) recipient mice that had been sublethally irradiated with 475 cGy one day before transplantation."
item32 "500,000 selected cells were injected via tail vein or retro-orbital route into female NSG (6-8 week old) recipient mice that had been sublethally irradiated with 475 cGy one day before transplantation."
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item36 "For the subcutaneous implantation model, 2 × 106 METTL3 stable knockdown Huh-7 cells or METTL3 overexpression MHCC97L cells were injected subcutaneously into BABL/cAnN-nude mice. For orthotopic implantation, wild-type and METTL3 knockout Huh-7 cells were luciferase labelled, and 2 × 106 cells were then injected orthotopically into the left liver lobe of nude mice."
item37 "For the subcutaneous implantation model, 2 × 106 METTL3 stable knockdown Huh-7 cells or METTL3 overexpression MHCC97L cells were injected subcutaneously into BABL/cAnN-nude mice. For orthotopic implantation, wild-type and METTL3 knockout Huh-7 cells were luciferase labelled, and 2 × 106 cells were then injected orthotopically into the left liver lobe of nude mice."
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item40 Performed an ex vivo genome wide CRISPR dropout screen (Screen 1) using Cas9-expressing mouse primary leukaemia cells driven by an MLL-AF9 fusion gene and a FLT3 internal tandem duplication.
item41 Performed an ex vivo genome wide CRISPR dropout screen (Screen 1) using Cas9-expressing mouse primary leukaemia cells driven by an MLL-AF9 fusion gene and a FLT3 internal tandem duplication.
item42 "Cells (5×106 cells in 200 uL) were suspended with 100 uL PBS and 100 uL Matrigel Matrix, and injected subcutaneously into the left armpit of each mouse."
item43 "Cells (5×106 cells in 200 uL) were suspended with 100 uL PBS and 100 uL Matrigel Matrix, and injected subcutaneously into the left armpit of each mouse."
item44 "Cells (5×106 cells in 200 uL) were suspended with 100 uL PBS and 100 uL Matrigel Matrix, and injected subcutaneously into the left armpit of each mouse."
item45 "Cells (5×106 cells in 200 uL) were suspended with 100 uL PBS and 100 uL Matrigel Matrix, and injected subcutaneously into the left armpit of each mouse."
item46 "For R-2HG injection mouse models, sensitive (NOMO-1 and MA9.3ITD) or resistant (MA9.3RAS) cells were injected into NSGS or NRGS intravenously, and then R-2HG (6mg/kg body weight) or PBS were injected once daily through tail vein for 12 consecutive days starting from day 11 post xeno-transplantation."
item47 "For R-2HG injection mouse models, sensitive (NOMO-1 and MA9.3ITD) or resistant (MA9.3RAS) cells were injected into NSGS or NRGS intravenously, and then R-2HG (6mg/kg body weight) or PBS were injected once daily through tail vein for 12 consecutive days starting from day 11 post xeno-transplantation."
item48 "For R-2HG injection mouse models, sensitive (NOMO-1 and MA9.3ITD) or resistant (MA9.3RAS) cells were injected into NSGS or NRGS intravenously, and then R-2HG (6mg/kg body weight) or PBS were injected once daily through tail vein for 12 consecutive days starting from day 11 post xeno-transplantation."
item49 "For R-2HG injection mouse models, sensitive (NOMO-1 and MA9.3ITD) or resistant (MA9.3RAS) cells were injected into NSGS or NRGS intravenously, and then R-2HG (6mg/kg body weight) or PBS were injected once daily through tail vein for 12 consecutive days starting from day 11 post xeno-transplantation."
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item51 "Lin- HSPCs were purified from BM of wildtype mice and 0.1×106 cells were seeded in 2 mL OP9 medium onto the OP9 cells with the addition of 10 ng/mL mouse IL-3, 10 ng/mL human IL-6, 10 ng/mL mouse IL-7, 10 ng/mL mouse Flt-3L, and 50 ng/mL mouse stem cell factor (SCF)."
item52 "Lin- HSPCs were purified from BM of wildtype mice and 0.1×106 cells were seeded in 2 mL OP9 medium onto the OP9 cells with the addition of 10 ng/mL mouse IL-3, 10 ng/mL human IL-6, 10 ng/mL mouse IL-7, 10 ng/mL mouse Flt-3L, and 50 ng/mL mouse stem cell factor (SCF)."
item53 "Either SiHa cells or FTO overexpressed SiHa cells were injected subcutaneously into the right flanks of 4- to 6-week-old female athymic nude mice (CAS, Beijing, China). The mice were divided into four groups (N = 6). After transplantation, tumor size was measured by caliper every other day. Tumor volume was calculated using the formula: volume = (length × width2)/2. When the tumor volumes reached 50 mm3, the animals were treated with intraperitoneal injection of cisplatin (3 mg/kg) every other day for seven times and local irradiation of 8 Gy one time."
item54 "Either SiHa cells or FTO overexpressed SiHa cells were injected subcutaneously into the right flanks of 4- to 6-week-old female athymic nude mice (CAS, Beijing, China). The mice were divided into four groups (N = 6). After transplantation, tumor size was measured by caliper every other day. Tumor volume was calculated using the formula: volume = (length × width2)/2. When the tumor volumes reached 50 mm3, the animals were treated with intraperitoneal injection of cisplatin (3 mg/kg) every other day for seven times and local irradiation of 8 Gy one time."
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item57 "To screen for meiotic defects, spermatocyte squash preparation and immunostaining for SYCP3 and γH2AX (described below) were carried out using testes from pubertal G3 males that were ≥15 dpp or from adult G3 males. At these ages, spermatocytes in the relevant stages of meiotic prophase I are abundant in normal mice."
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item59 "To build the mouse model for POI, cyclophosphamide (CTX) (Sigma, St. Louis, MO) was used a high-dose treatment (120 mg/kg, 2 weeks)."
item60 "To build the mouse model for POI, cyclophosphamide (CTX) (Sigma, St. Louis, MO) was used a high-dose treatment (120 mg/kg, 2 weeks)."
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item88 "After being fed with high-fat diet for 4 weeks, mice were given twice vena caudalis injection of control siRNA or Cidec siRNA (50 ug/mouse) mixed with liposome. Liposomes were prepared as described elsewhere."
item89 "After being fed with high-fat diet for 4 weeks, mice were given twice vena caudalis injection of control siRNA or Cidec siRNA (50 ug/mouse) mixed with liposome. Liposomes were prepared as described elsewhere."
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item100 "Mice were anesthetized after 24 h of fasting, and 5 U of human insulin (Humalin R; Eli Lilly) was injected into the inferior vena cava. After 5 min, the liver and hind limb muscles were dissected and immediately frozen in liquid nitrogen."
item101 "Mice were anesthetized after 24 h of fasting, and 5 U of human insulin (Humalin R; Eli Lilly) was injected into the inferior vena cava. After 5 min, the liver and hind limb muscles were dissected and immediately frozen in liquid nitrogen."
item102 "Mice were anesthetized after 24 h of fasting, and 5 U of human insulin (Humalin R; Eli Lilly) was injected into the inferior vena cava. After 5 min, the liver and hind limb muscles were dissected and immediately frozen in liquid nitrogen."
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item104 All pups were genotyped and two Mettl3flox/+ founder mice were obtained.
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item108 "The animals were kept in standard laboratory polycarbonate cages in controlled ambient temperature at 23℃ ± 1℃ with relative humidity of 50% ± 10%, and a light-dark cycle of 12/12 h. The mice had free access to standardized pellet food and tap water. After 1-week of habituation, the animals were randomly divided into the following 4 groups: control group, 0.5 mg/l arsenite group, 5 mg/l arsenite group and 50 mg/l arsenite group (n = 16 per group). The mice were exposed to arsenite via drinking water for 6 months."
item109 "We quantified m6A levels in RNA extracted from failing human (both ischemic and non-ischemic), pig and mouse (post-myocardial infarction ischemic) hearts and compared them to m6A in non-failing human and sham surgical controls respectively. We detected significantly elevated levels of m6A in both total and polyA+ RNA extracted from human, pig and mouse failing left ventricular (LV) explants compared to non-failing or sham."
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item111 "For the rescue experiment, LSK cells were sorted from wt and Ythdf2 KO mouse BM and cultured overnight in StemSpan SFEM medium (Stem Cell Technologies) supplemented with 10 ug/mL heparin (Sigma), 0.5 × penicillin/streptomycin (Sigma), 10 ng/mL recombinant mouse (rm) stem cell factor (SCF), and 20 ng/mL Tpo 75 at 37 ℃ in a 5% CO2 5% O2 atmosphere."
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item116 "Utilized three Cre transgenic mouse lines (Mx1-Cre, Ert-Cre and Vav-Cre) to cross with Ythdf2fl/fl mice to deplete the expression of YTHDF2 specifically in adult HSCs of mouse bone marrow (BM), respectively."
item117 "4×106 HEC-1-A endometrial cancer cells (shCtrl, shMETTL3, wild-type, METTL14+/-, or METTL14+/- rescued with wild-type or mutant METTL14) were injected intraperitoneally into 5 week old female athymic nude mice (Foxn1nu, Harlan; n=10 per group)."
item118 "4×106 HEC-1-A endometrial cancer cells (shCtrl, shMETTL3, wild-type, METTL14+/-, or METTL14+/- rescued with wild-type or mutant METTL14) were injected intraperitoneally into 5 week old female athymic nude mice (Foxn1nu, Harlan; n=10 per group)."
item119 "4×106 HEC-1-A endometrial cancer cells (shCtrl, shMETTL3, wild-type, METTL14+/-, or METTL14+/- rescued with wild-type or mutant METTL14) were injected intraperitoneally into 5 week old female athymic nude mice (Foxn1nu, Harlan; n=10 per group)."
item120 "4×106 HEC-1-A endometrial cancer cells (shCtrl, shMETTL3, wild-type, METTL14+/-, or METTL14+/- rescued with wild-type or mutant METTL14) were injected intraperitoneally into 5 week old female athymic nude mice (Foxn1nu, Harlan; n=10 per group)."
item121 "2 × 106 tumor cells (OVCAR3-METTL3 and OVCAR3-Ctrl) or 1 × 106 tumor cells (SKOV3-shMETTL3-1, SKOV3-shMETTL3-2 and SKOV3-shNC) were suspended in 200 uL of RPMI 1640 complete culture medium with 25% Matrigel (BD Biosciences) and inoculated subcutaneously into the right flank of the nude mice."
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item126 "For tumor xenograft studies, MDA-MB-231 cells transfected with scrambled-siRNA or METTL14-siRNA or ALKBH5-siRNA (2 × 106) were mixed with Matrigel and injected subcutaneously in the flank of 6-week-old female athymic nude mice."
item127 "For tumor xenograft studies, MDA-MB-231 cells transfected with scrambled-siRNA or METTL14-siRNA or ALKBH5-siRNA (2 × 106) were mixed with Matrigel and injected subcutaneously in the flank of 6-week-old female athymic nude mice."
item128 HNF4a knockout (HNF4a-/-) mice were generated using CRISPR/Cas9 in C57BL/6 mice.
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item130 5 × 106 of HEP3B and SMMC7721 stable cells were resuspended in 0.1 ml of PBS and subcutaneously injected into the flank of mice.
item131 Mettl3fl/+ mice with C57BL6/J background were generated using CRISPR-Cas9 systems.
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item136 "BALB/c-nu mice (4-6 weeks old, female) were purchased from Charles River Laboratories (Beijing, China), and SUNE1-vector-luciferase or SUNE1-ZNF750-luciferase cells (1 × 106) were subcutaneously injected into the dorsal or ventral flank."
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item138 Mettl3 fl/fl mice were crossed with mice expressing cre recombinase under the control of the cardiac-specific Myh7 promoter (Beta-myosin heavy chain [Beta-MHC]) to obtain heart-restricted deletion of Mettl3 (METTL3-cKO; cKO).
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item140 "Five- to 6-week-old male athymic nude mice purchased by Charles River were used for the xenograft model. 769-P cells stably expressing Ctrl, FTO and FTO-mut were trypsinized and washed twice to thrice with standardized PBS, and then, 5 × 106 cells in 100 uL of PBS was subcutaneously injected into the flanks of the mice (five mice per group). Mice were monitored twice every week for tumour growth, and tumour diameters were measured using a caliper."
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item154 "Positive ES cell clones were expanded and injected into C57BL/6J blastocysts to generate the chimeric offspring. The chimeric mice were mated with C57BL/6J mice to obtain the lnc-Dpf3 floxed heterozygous mouse (lnc-Dpf3fl/+). The lnc-Dpf3fl/+ mice were bred with C57BL/6J mice expressing the Cre recombinase under the control of the Itgax promoter (Itgax-cre mice; The Jackson Laboratory) to generate lnc-Dpf3 floxed heterozygous mouse with Itgax-cre allele (lnc-Dpf3fl/+Itgax-cre+). These mice were intercrossed to generate DC-specific lnc-Dpf3-deficient mice (lnc-Dpf3fl/flItgax-cre+) used for this study. Hif1afl/fl mice, a kindly gift from Dr. Ming Zhang (Renji Hospital, Medical College of Shanghai Jiaotong University, China) were crossed with Itgax-cre mice to generate DC-specific HIF-1-Alpha-deficient mice (Hif1afl/flItgax-cre+). All mice were maintained under specific pathogen-free conditions and used at 6-8 weeks of age."
item155 "Two weeks after the stereotaxic surgery, all the animals were intraperitoneally injected with apomorphine at a dose of 0.5 mg/kg to induce the contralateral rotations. Ten minutes after the injection, a video was used to record the rotations of each rat for 20 min. Only those 6-OHDA induced rats showing robust contralateral turning (>7 turns/min) that were injected with 6-OHDA were used in subsequent experiments."
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item158 "To cause I/R injury, mice were subjected to 30 min of LAD ischemia followed by 60 min of reperfusion."
item159 "To cause I/R injury, mice were subjected to 30 min of LAD ischemia followed by 60 min of reperfusion."
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item168 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
item169 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
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item172 MDSCs were from C57BL/6 or C57BL/6 IL6 KO mice. B16 tumor cells were injected into wild-type (WT) and IL6 KO mice. PBS was used as a control.
item173 "For the subcutaneous implantation model, 5 4-week-old female Balb/c mice were randomly grouped and injected with 1 × 106 shCtrl, shFTO or shFTO/shBNIP3 KD 4 T1 cells. For tumor metastasis mouse model, 5 4-week-old female Balb/c mice were randomly grouped and injected with 1 × 106 shCtrl, shFTO or shFTO/shBNIP3 KD 4 T1 cells via tail vein. For orthotopic xenograft mouse model, 5 4-week-old female NOD/SCID mice were randomly grouped."
item174 HPAF-II cells (2.0 × 106 cells/site) stably transfected with sh-EGFP or sh-UCA1 were subcutaneously injected into 4-week-old nude mice to generate xenografts. The tumor volume was measured every week after injection and calculated using the following formula: length × (width2)/2.
item175 HPAF-II cells (2.0 × 106 cells/site) stably transfected with sh-EGFP or sh-UCA1 were subcutaneously injected into 4-week-old nude mice to generate xenografts. The tumor volume was measured every week after injection and calculated using the following formula: length × (width2)/2.
item176 HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 uL normal medium + 100 uL Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth.
item177 HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 uL normal medium + 100 uL Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth.
item178 HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 uL normal medium + 100 uL Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth.
item179 HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 uL normal medium + 100 uL Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth.
item180 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
item181 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
item182 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
item183 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
item184 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
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item186 Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank.
item187 THP-1 cells transduced with CTL or KD lentiviruses were tail vein injected into non-irradiated 12 week-old female non-obese diabetic (NOD)/LtSz-severe combined immune-deficiency (SCID) IL-2Rγcnull (NSG) mice (1x106 cells per 200 uL per mouse).
item188 "All animal experiments complied with Zhongshan School of Medicine Policy on Care and Use of Laboratory Animals. For subcutaneous transplanted model, sh-control and sh-METTL3 Huh7 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL of PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice to investigate tumor growth."
item189 "All animal experiments complied with Zhongshan School of Medicine Policy on Care and Use of Laboratory Animals. For subcutaneous transplanted model, sh-control and sh-METTL3 Huh7 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL of PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice to investigate tumor growth."
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item195 Mice were anesthetized and shaved on both flanks and injected subcutaneously with 4 × 106 tumor cells in 200 uL of PBS.
item196 "1 × 105 parental HT29-shNC cells, HT29-shYTHDF1 cells, HT29-shNC colonospheres, and HT29-shYTHDF1 colonospheres were inoculated subcutaneously into the left inguinal folds of the nude mice."
item197 1.5 × 106 TFK1 cells with or without FTO overexpression were injected subcutaneously into the left and right flanks of 6-week-old female athymic nude mice.
item198 2 × 106 cells were suspended in 150 uL DMEM. The A431 cells (scrambled group and shRNA1 group) were injected subcutaneously into the flanks of nude mice.
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item200 "The right knee joint of each OA mouse was injected with 1U of type VII collagenase over two consecutive days to obtain experimental OA joint, and the control mice received the equal volume of physiological saline."
item201 "The right knee joint of each OA mouse was injected with 1U of type VII collagenase over two consecutive days to obtain experimental OA joint, and the control mice received the equal volume of physiological saline."
item202 "About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group)."
item203 "About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group)."
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item207 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
item208 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
item209 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
item210 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
item211 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
item212 "When the tumors reached a volume of 80-100 mm3, mice were treated with anti-PD-1 or isotype control antibody (200 ug/mouse) by i.p. injection, every other day for three times. For IFNγ blockade treatment, C57BL/6 mice were treated with anti-IFNγ antibody or isotype control IgG (250 ug/mouse) every other day after tumor cell inoculation."
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item215 The Metastatic Bone Tumor Model was established via injecting HOS cells into the right tibia of each animal.
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item230 "Xenograft tumors were generated by injection (5.0 × 106 cells/injection) of shMTHFD2 786-O and CAKI-1 lines and their respective shControl cell lines (n = 6 per line, sample size calculated for 80% power to detect a twofold difference with 95% confidence) subcutaneously into 6-week-old male Nu/J mice."
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item233 "For the tumor growth model, 1 × 106 HNE1-Scrambled or HNE1-shFAM2225A 2# cells were injected into the axilla of the mice, and the tumor size was measured every 3 days."
item234 "For the tumor growth model, 1 × 106 HNE1-Scrambled or HNE1-shFAM2225A 2# cells were injected into the axilla of the mice, and the tumor size was measured every 3 days."
item235 Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid (MA2) to inhibit the demethylase activity of FTO.
item236 Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid (MA2) to inhibit the demethylase activity of FTO.
item237 Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid (MA2) to inhibit the demethylase activity of FTO.
item238 Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid (MA2) to inhibit the demethylase activity of FTO.
item239 .
item240 .
item241 .
item242 .
item243 .
item244 .
item245 .
item246 .
item247 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item248 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item249 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item250 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item251 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item252 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item253 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item254 "Hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were generated by crossing mice with TBG-Cre Tg mice. METTL3 flox (METTL3 fl/fl) and hepatocyte-specific METTL3 knockout mice (TBG-Cre, METTL3 fl/fl) were used for experiments."
item255 "For the subcutaneous implantation model, 1 × 107 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice."
item256 "For the subcutaneous implantation model, 1 × 107 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice."
item257 "For the subcutaneous implantation model, 1 × 107 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice."
item258 "For the subcutaneous implantation model, 1 × 107 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice."
item259 .
item260 .
item261 "FtoKO mice were backcrossed to WT C57BL/6 mice to remove Cre and bred to homozygosity. Results are reported for male mice on the same genetic background (C57BL6/J). For the diet-induced bone loss studies, mice were fed a 60% high-fat diet (D12492, Research Diets) from 6 wk of age to 24 wk. Genotyping strategies are available upon request. NBD (KKKKKKKKGGTALDWSWLQTE) with the Trp to Ala substitutions designed to render the peptide inactive underlined, was a gift from D.C.G. and dissolved in water before use. Next, 10 mg/kg NBD was intraperitoneally injected in 29-wk old FtoOc KO mice every other day for 9 d. One day after the last injection, bone was harvested for analysis of DNA damage."
item262 "3 × 106 treated HCC cells resuspended in 100 uL PBS were subcutaneously injected to the left flank of the mice, which were randomly divided into several groups. Tumor sizes were measured every 3 to 5 days."
item263 "Mice were maintained at 22 ± 2 ℃ with a humidity of 35 ± 5% under a 12 h light and 12 h dark cycle, with free access to water and food. For the HFD experiment, female control (Ftoflox/flox) and adipose-selective fto knockout (Fabp4-Cre Ftoflox/flox, fto-AKO) mice were fed with high-fat diet (60% fat in calories; Research Diets, D12492) for the desired periods of time, and food intake and body weight were measured every week after weaning (at 3 weeks of age)."
item264 "Mice were maintained at 22 ± 2 ℃ with a humidity of 35 ± 5% under a 12 h light and 12 h dark cycle, with free access to water and food. For the HFD experiment, female control (Ftoflox/flox) and adipose-selective fto knockout (Fabp4-Cre Ftoflox/flox, fto-AKO) mice were fed with high-fat diet (60% fat in calories; Research Diets, D12492) for the desired periods of time, and food intake and body weight were measured every week after weaning (at 3 weeks of age)."
item265 "Mice were maintained at 22 ± 2 ℃ with a humidity of 35 ± 5% under a 12 h light and 12 h dark cycle, with free access to water and food. For the HFD experiment, female control (Ftoflox/flox) and adipose-selective fto knockout (Fabp4-Cre Ftoflox/flox, fto-AKO) mice were fed with high-fat diet (60% fat in calories; Research Diets, D12492) for the desired periods of time, and food intake and body weight were measured every week after weaning (at 3 weeks of age)."
item266 "Mice were maintained at 22 ± 2 ℃ with a humidity of 35 ± 5% under a 12 h light and 12 h dark cycle, with free access to water and food. For the HFD experiment, female control (Ftoflox/flox) and adipose-selective fto knockout (Fabp4-Cre Ftoflox/flox, fto-AKO) mice were fed with high-fat diet (60% fat in calories; Research Diets, D12492) for the desired periods of time, and food intake and body weight were measured every week after weaning (at 3 weeks of age)."
item267 "Mice were maintained at 22 ± 2 ℃ with a humidity of 35 ± 5% under a 12 h light and 12 h dark cycle, with free access to water and food. For the HFD experiment, female control (Ftoflox/flox) and adipose-selective fto knockout (Fabp4-Cre Ftoflox/flox, fto-AKO) mice were fed with high-fat diet (60% fat in calories; Research Diets, D12492) for the desired periods of time, and food intake and body weight were measured every week after weaning (at 3 weeks of age)."
item268 "Eight-to-twelve-week-old female C57BL/6 mice purchased from Jackson Laboratories were immunized subcutaneously at the base of the tail with 100 ug per mouse of OVA protein (Invivogen, vac-pova) adjuvanted with 25 ug of HMW vaccine grade Poly I:C (Invivogen, vac-pic), 25 ug of circular RNA alone or with in vivo-jetPEI (Polyplus Transfection, 201-10G), 25 ug of modified RNA alone or with in vivo-jet PEI."
item269 "Eight-to-twelve-week-old female C57BL/6 mice purchased from Jackson Laboratories were immunized subcutaneously at the base of the tail with 100 ug per mouse of OVA protein (Invivogen, vac-pova) adjuvanted with 25 ug of HMW vaccine grade Poly I:C (Invivogen, vac-pic), 25 ug of circular RNA alone or with in vivo-jetPEI (Polyplus Transfection, 201-10G), 25 ug of modified RNA alone or with in vivo-jet PEI."
item270 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
item271 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
item272 "200 uL PBS containing 1×107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
item273 "200 uL PBS containing 1×107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
item274 "Rats inhaled CB at dose of 0, 5 or 30 mg/m3 for 28 days, 6 h/day, respectively. The rats inhaled CB at dose of 0 or 30 mg/m3 for 14 days, 28 days and 90 days, respectively. In vitro experiments, the normal human bronchial epithelial cell line (16HBE) was treated with CB (0, 50, 100 and 200 ug/mL) for 24 h."
item275 .
item276 "For the animal studies, 6-week-old male athymic BALB/c nude mice were implanted with modified ALKBH5 expressing Hct116 or RKO cells (105/ml, 200 ml) via lateral tail vein injection. All the mice were killed after 6 weeks, and the lungs were subjected to H&E staining."
item277 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item278 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item279 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item280 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item281 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item282 "For subcutaneous tumor model, each mouse was injected subcutaneously in the right flank with 2 × 106 U87MG cells (METTL3-KD or control) in 100 uL PBS."
item283 Induced AKI in c57BL/6 mice by intraperitoneal cisplatin injection and treated the animal with vehicle or an FTO inhibitor meclofenamic acid (MA) for 3 days.
item284 Induced AKI in c57BL/6 mice by intraperitoneal cisplatin injection and treated the animal with vehicle or an FTO inhibitor meclofenamic acid (MA) for 3 days.
item285 .
item286 Mice 8 weeks after splenic portal vein injection of BGC823 cells with METTL3 overexpression or vector-transfected cells.
item287 Mice 8 weeks after splenic portal vein injection of BGC823 cells with METTL3 overexpression or vector-transfected cells.
item288 .
item289 The luciferase signal intensity from days 7 to 42 is on equivalent scales in the models. Bioluminescent flux (photons/s/cm2/steradian) was determined for the lung metastases.
item290 .
item291 2 × 106 cells suspended in 40 uL PBS were injected into the inferior hemispleen into each 6-week-old BALB/c nude mouse.
item292 "The transfected cells (2×106) were directly subcutaneously injected in to flank of mice. The width and length were measured every six days. After three weeks, the mice were killed and the necropsies were weighted."
item293 .
item294 .
item295 .
item296 .
item297 "According to the method published previously , exponentially growing MG63 and MG63/DXR were harvested, counted with trypan blue to show cells with at least 95% viability, and then resuspended at a final amount from 5 × 106 to 5 × 103 in 100 uL Matrigel (Corning, NY, USA) before being injected subcutaneously into 5-week-old female nude mice.The mice were monitored once every 3 days until the 60th day, and euthanized humanely after the experiment."
item298 "According to the method published previously , exponentially growing MG63 and MG63/DXR were harvested, counted with trypan blue to show cells with at least 95% viability, and then resuspended at a final amount from 5 × 106 to 5 × 103 in 100 uL Matrigel (Corning, NY, USA) before being injected subcutaneously into 5-week-old female nude mice.The mice were monitored once every 3 days until the 60th day, and euthanized humanely after the experiment."
item299 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item300 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item301 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item302 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item303 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item304 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item305 "Mice were treated via nasal inhalation of adenovirus carrying Cre recombinase (5 × 106 p.f.u for Ad-Cre, Biowit Inc., Shenzhen, Guangdong), and were then killed at indicated times for gross inspection and histopathological examination."
item306 1 × 107 A549 cells were subcutaneously implanted into 4-week-old NOD/SCID mice.
item307 "The experimental rats received intraperitoneal injection with IS at a dosage of 100 mg/kg/48 h for 8,16,24 weeks. The control rats (n = 5) received same volume of phosphate-buffered saline injection every 48 h for 8,16,24 weeks."
item308 .
item309 .
item310 .
item311 .
item312 .
item313 .
item314 "Approximately 1 × 106 melanoma cells from each group were injected subcutaneously into the right side of the abdomen of BALB/c nude mice (male, 6 weeks old)."
item315 .
item316 .
item317 "For the engraftment experiments, 1×103 1×106 cells were injected into tail veins of non-irradiated 6-10 week-old female mice in 100 uL of DPBS per mouse. No blinding or randomization was applied to mice experiments. Routinely, each in vivo experiment was performed with three technical replicates (three mice per group) and independently repeated two to three times for each cell line."
item318 "For the engraftment experiments, 1×103 1×106 cells were injected into tail veins of non-irradiated 6-10 week-old female mice in 100 uL of DPBS per mouse. No blinding or randomization was applied to mice experiments. Routinely, each in vivo experiment was performed with three technical replicates (three mice per group) and independently repeated two to three times for each cell line."
item319 "For the engraftment experiments, 1×103 1×106 cells were injected into tail veins of non-irradiated 6-10 week-old female mice in 100 uL of DPBS per mouse. No blinding or randomization was applied to mice experiments. Routinely, each in vivo experiment was performed with three technical replicates (three mice per group) and independently repeated two to three times for each cell line."
item320 .
item321 "For the orthotopic models, 2 × 106 cells with negative control (NC, sh-NC), sh-1 or sh-2 in 0.5 mL of PBS were subcutaneously injected into the dorsal flank of 2 mice respectively. Then 15 mice were separated into 3 groups (sh-NC, sh-1 and sh-2), of which the tumor pieces were tied to the base of the ceca. The growth of the tumors was monitored every 2 weeks after intraperitoneal injection of D-luciferin with a Xenogen IVIS 100 Bioluminescent Imaging System."
item322 "DANCR KO or empty vector control were harvested and then mixed with matrigel (1:1) (BD Biosciences). Three different numbers of cells (1 × 104, 1 × 105, and 5 × 105 cells) were subcutaneously injected into nude mice, five animals per group."
item323 Equal number of PC-3 cells was injected subcutaneously into right flank.
item324 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item325 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item326 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item327 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item328 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item329 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item330 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item331 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item332 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
item333 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
item334 .
item335 .
item336 "Six-week-old BALB/c nude mice were purchased from the College of Veterinary Medicine, Yang Zhou University. For the xenografted tumor model, 1 × 107 HCT116 cells in 0.2 mL PBS were subcutaneously injected into BALB/c nude mice, which were randomly divided into four groups (six mice per group). The volume of the tumors was calculated with the following equation: V = 0.5 × (length × width2). For metastasis experiments, 2 × 106 cells in 0.2 mL PBS were injected into the tail vein of nude mice, which were randomly divided into four groups (six mice per group)."
item337 .
item338 5 × 106 cells were subcutaneously injected into the left or right flank of each mouse.
item339 .
item340 .
item341 .
item342 .
item343 .
item344 .
item345 .
item346 .
item347 .
item348 "2 × 106 A549 cells stably transfected with shRNA-ALKBH5 were injected into the flank of male athymic BALB/c nude mice (4-5 weeks old, 10 mice)."
item349 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
item350 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
item351 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
item352 .
item353 "Cells transfected with miR-186 overexpression lentivirus (Lenti-miR-186), miR-186 inhibitor (Lenti-anti-miR-186), Lenti-miR-186 & METTL3 overexpression plasmid (Lenti-METTL3), Lenti-anti-miR-186 & METTL3 shRNA (sh-METTL3) or empty lentivirus control (Lenti-NC) were subcutaneously injected into the lower flank of nude mice."
item354 .
item355 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item356 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item357 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item358 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item359 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item360 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item361 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item362 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item363 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item364 Male SPF BALB/c mice (qls02-0202) were purchased from Qinglongshan animal breeding farm. Mice were sacrificed by CO2 asphyxiation and testes were obtained for following histopathological analyses.
item365 .
item366 .
item367 5 × 106 cells in PBS were injected subcutaneously into one side of the posterior flanks of Balb/C nude mice at 6-8 weeks old.
item368 Indicated stable 143B cells were subcutaneously injected into nude mice.
item369 Indicated stable 143B cells were subcutaneously injected into nude mice.
item370 .
item371 .
item372 .
item373 Stable down-regulated FTO cells were prepared in Eca-109 and KYSE150 and subcutaneously injected into the flank of nude mouse with 2 × 106 cells per mouse.
item374 LoVo cells (5 × 106 cells/ 200 uL PBS) stably transfected with METTL3 knockdown lentiviral vector or control vector were respectively injected subcutaneously into the left flank of each mouse.
item375 .
item376 .
item377 .
item378 .
item379 "Gastric cancer cell line MGC803 (shRNA-NC or shKIAA1429; 1 × 107) was injected subcutaneously into the armpit of BALB/c nude mice (5-week-old, male, n = 4 for each group). Tumor growth was monitored at 3-day intervals."
item380 .
item381 .
item382 .
item383 "For the experiments, mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into indicated groups (five mice per group). To assess the in vivo effects of cycloleucine, the xenografted tumors had reached approximately 5 mm in diameter from mice and then these xenografted mice were feed with Vehicle or cycloleucine (25 mg/kg twice weekly) and tumor volume were measured every 3 day. Tumor volume was estimated as 0.5 × a2 × b (where a and b represent a tumors short and long diameter, respectively). Mice were euthanized after 7 weeks and the tumors were measured a final time."
item384 "For the experiments, mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into indicated groups (five mice per group). To assess the in vivo effects of cycloleucine, the xenografted tumors had reached approximately 5 mm in diameter from mice and then these xenografted mice were feed with Vehicle or cycloleucine (25 mg/kg twice weekly) and tumor volume were measured every 3 day. Tumor volume was estimated as 0.5 × a2 × b (where a and b represent a tumors short and long diameter, respectively). Mice were euthanized after 7 weeks and the tumors were measured a final time."
item385 "For the experiments, mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into indicated groups (five mice per group). To assess the in vivo effects of cycloleucine, the xenografted tumors had reached approximately 5 mm in diameter from mice and then these xenografted mice were feed with Vehicle or cycloleucine (25 mg/kg twice weekly) and tumor volume were measured every 3 day. Tumor volume was estimated as 0.5 × a2 × b (where a and b represent a tumors short and long diameter, respectively). Mice were euthanized after 7 weeks and the tumors were measured a final time."
item386 .
item387 "For liver metastasis model, mice were anaesthetized and an incision was made through the skin and peritoneum to expose the spleen. 1 × 106 HCT116 cells were injected into the spleen (n = 4 each group)."
item388 "For liver metastasis model, mice were anaesthetized and an incision was made through the skin and peritoneum to expose the spleen. 1 × 106 HCT116 cells were injected into the spleen (n = 4 each group)."
item389 "For the subcutaneous implantation model, UM-UC-3 cells (2 × 106 cells per mouse) stably METTL3 knocked down (shMETTL3-1, shMETTL3-2) were injected into the flanks of mice."
item390 "For the subcutaneous implantation model, UM-UC-3 cells (2 × 106 cells per mouse) stably METTL3 knocked down (shMETTL3-1, shMETTL3-2) were injected into the flanks of mice."
item391 "For the subcutaneous implantation model, UM-UC-3 cells (2 × 106 cells per mouse) stably METTL3 knocked down (shMETTL3-1, shMETTL3-2) were injected into the flanks of mice."
item392 "For the subcutaneous implantation model, UM-UC-3 cells (2 × 106 cells per mouse) stably METTL3 knocked down (shMETTL3-1, shMETTL3-2) were injected into the flanks of mice."
item393 The stable transfection of SCC25 cells (1 × 107 cells in 0.1 mL) with lenti-sh-METTL3 or blank vectors was injected subcutaneously into BALB/c nude mice.
item394 The stable transfection of SCC25 cells (1 × 107 cells in 0.1 mL) with lenti-sh-METTL3 or blank vectors was injected subcutaneously into BALB/c nude mice.
item395 "All mice were housed at 21℃ ± 1℃ with a humidity of 55% ± 10% and a 12-hour light/dark cycle. The high-fat diets (HFDs), containing 60% kcal from fat, 20% kcal from carbohydrate, and 20% kcal from protein."
item396 "All mice were housed at 21℃ ± 1℃ with a humidity of 55% ± 10% and a 12-hour light/dark cycle. The high-fat diets (HFDs), containing 60% kcal from fat, 20% kcal from carbohydrate, and 20% kcal from protein."
item397 "All mice were housed at 21℃ ± 1℃ with a humidity of 55% ± 10% and a 12-hour light/dark cycle. The high-fat diets (HFDs), containing 60% kcal from fat, 20% kcal from carbohydrate, and 20% kcal from protein."
item398 "All mice were housed at 21℃ ± 1℃ with a humidity of 55% ± 10% and a 12-hour light/dark cycle. The high-fat diets (HFDs), containing 60% kcal from fat, 20% kcal from carbohydrate, and 20% kcal from protein."
item399 "Male BALB/c nude mice (5 weeks old) were subcutaneously inoculated in the right axillary fossa with 200 uL (1 × 106 cells) of SIRT1-overexpressing Hep3B cells, SIRT1- and FTO-overexpressing Hep3B cells, shRNA-SIRT1-transfected HepG2 cells, shRNA-SIRT1- and shRNA-FTO-transfected HepG2 cells, and control cells."
item400 .
item401 .
item402 1×106 ES-2-Scrambled or ES-2-shRHPN1-AS1 cells were injected subcutaneously on the right side of the mice.
item403 .
item404 .
item405 .
item406 Injected YY1BM intratumorally into ESCC tumors grafted in nude mice and analyzed the survival time.
item407 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
item408 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
item409 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
item410 .
item411 .
item412 .
item413 "For the unilateral ureteral obstruction (UUO) model, male C57BL/6J mice at 8 weeks of age (20-22 g body weight) were first anaesthetized with pentobarbital sodium (50 mg/kg) via intraperitoneal injection. Then, the left ureter was ligated using 3-0 silk and a left lateral incision."
item414 "Both sh-control and sh- ERRγ HepG2/ADR cells (5 × 106 per mouse, n=5 for each group) were diluted in 200uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice."
item415 "Both sh-control and sh- ERRγ HepG2/ADR cells (5 × 106 per mouse, n=5 for each group) were diluted in 200uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice."
item416 "Both sh-control and sh- ERRγ HepG2/ADR cells (5 × 106 per mouse, n=5 for each group) were diluted in 200uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice."
item417 .
item418 .
item419 .
item420 .
item421 .
item422 .
item423 .
item424 .
item425 .
item426 .
item427 "1 × 106 HCT-116-Luc-LINC00266-1-ORF-Flag, HCT-116-Luc- LINC00266-1-5'U, or HCT-116-Luc-LINC00266-1-MT cells were subcutaneously injected into the dorsal flanks of each BALB/c nude mouse (n = 6)."
item428 .
item429 .
item430 "All the mice were randomly divided into five groups of four mice each, including PC3-shNC, PC3-shMETTL3-1/2, LNCaP-vector and LNCaP-METTL3 group."
item431 .
item432 .
item433 .
item434 "A longitudinal incision was made on the skin and the muscles were separated to expose the femur. A transverse osteotomy was performed in the mid-diaphysis of the femur, and the bones were stabilized by inserting a 23-gauge intramedullary needle. Equal amounts (100 uL) of phosphate-buffered saline (PBS), plasmid METTL3 and agomiR-7212-5p (10 mg/kg body weight) were locally injected into the femoral fracture site."
item435 "Established cohorts of mice bearing tumour xenografts driven by PTEN-deficient BxPC-3 and PANC-1 cells with PIK3CB overexpression. When tumours grew to ~300 mm3, mice were grouped and administered with vehicle (DMSO) or KIN-193 via intraperitoneal injection (20 mg/kg) once daily."
item436 "Established cohorts of mice bearing tumour xenografts driven by PTEN-deficient BxPC-3 and PANC-1 cells with PIK3CB overexpression. When tumours grew to ~300 mm3, mice were grouped and administered with vehicle (DMSO) or KIN-193 via intraperitoneal injection (20 mg/kg) once daily."
item437 "Established cohorts of mice bearing tumour xenografts driven by PTEN-deficient BxPC-3 and PANC-1 cells with PIK3CB overexpression. When tumours grew to ~300 mm3, mice were grouped and administered with vehicle (DMSO) or KIN-193 via intraperitoneal injection (20 mg/kg) once daily."
item438 "Established cohorts of mice bearing tumour xenografts driven by PTEN-deficient BxPC-3 and PANC-1 cells with PIK3CB overexpression. When tumours grew to ~300 mm3, mice were grouped and administered with vehicle (DMSO) or KIN-193 via intraperitoneal injection (20 mg/kg) once daily."
item439 .
item440 .
item441 Female athymic BALB/c nude mice (4-week-old) were provided (SLAC Laboratory Animal Co. Ltd.). The animals were raised in a pathogen-free animal laboratory and randomly divided into the control or experimental group (six mice in each group).
item442 .
item443 .
item444 .
item445 .
item446 "For the drug-resistant subcutaneous tumor models, drug administration was adopted when the tumors reached about 50 mm3 in size, at which point mice were randomized for treatment with DMSO(intraperitoneally) or sorafenib (50 mg/kg/every 2 days, intraperitoneally). For the patient-derived tumor xenograft model, drug administration began 4 weeks after tumors reached about 100 mm3 in size with sorafenib (50 mg/kg/every 3 days, intraperitoneally) or siCtrl/siMETTL3 intratumor injection."
item447 .
item448 A total of 2 × 106 cells was mixed with 0.2 ml PBS (pH 7.4) and 30% (v/v) Matrigel matrix (BD Biosciences).
item449 A total of 2 × 106 cells was mixed with 0.2 ml PBS (pH 7.4) and 30% (v/v) Matrigel matrix (BD Biosciences).
item450 .
item451 "GRNA and Cas9 mRNA were mixed at concentration of 50 and 100 ng/ul, respectively, and injected to the cytoplasm of one-cell-stage embryos of C57BL/6 genetic background."
item452 "Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
item453 .
item454 .
item455 .
item456 .
item457 .
item458 .
item459 .
item460 About 5 × 106 cells transfected with pc-YTHDF1 in SHG-44 cell (si-YTHDF1 in U87 cell) were suspended in 0.1 mL of PBS and injected subcutaneously into nude mice.
item461 "The OE21 cells were stably transfected with sh-ALKBH5 (#1), sh-ALKBH5 (#2) or sh-scramble as negative control and injected (2 × 106 cells/mouse in 100 uL volume) subcutaneously into the back of male athymic BALB/c nude mice (6 weeks old, Japan SLC)."
item462 1×106/mouse cells were suspended in 50 uL of serum-free DMEM and subcutaneously injected into the flank of each mouse. Tumor diameters and volume (length × width2 × 0.5236) were calculated every other day using calipers.
item463 METTL3 stable knockdown or overexpression HCT116 cells were collected and resuspended at a density of 5 × 106 or 3 × 106 cells per 150 uL PBS.
item464 .
item465 .
item466 .
item467 .
item468 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group).
item469 "The nude mice were randomly grouped into 2 groups, of 5 mice each; 786-0 cells (7×106 in 100 L PBS) were stabilized with ALKBH5 knockdown lentiviral transfection vector (shALKBH5) or scramble vector (SCR) via subcutaneous injection into the left armpit of each mouse."
item470 .
item471 .
item472 "After a median incision on the neck, the left common carotid artery (CCA), internal carotid artery (ICA), and external carotid artery (ECA) were isolated. The left CCA and the ECA were ligated."
item473 "Female nude, athymic, BALB/c-nu/nu mice (6-7 weeks old; Harlan) were injected subcutaneously (s.c.) with serially diluted numbers (5,000, 10,000, or 20,000) of OVCAR5_GFP or OVCAR5_FTO cells mixed with Matrigel (Fisher Scientific). Mice were monitored bi-weekly and time to tumor initiation and tumor growth were recorded."
item474 A total of 5.0 × 106 normal (shNC) or METTL3-depleted (shMETTL3) CAL-27 cells were inoculated subcutaneously into the dorsal flank of the nude mice in 100 uL PBS.
item475 A total of 5.0 × 106 normal (shNC) or METTL3-depleted (shMETTL3) CAL-27 cells were inoculated subcutaneously into the dorsal flank of the nude mice in 100 uL PBS.
item476 .
item477 1 × 107 Bel-7404 cells in 200 uL PBS were injected into the right flank of nude mice.
item478 .
item479 .
item480 .
item481 "Previously prepared U87 cells (5 × 106 cells, 60 uL) stably infected with miRNA were injected subcutaneously in the right flank of the mice, whereas control U87 cells infected with empty vector were injected in the left flank."
item482 "Previously prepared U87 cells (5 × 106 cells, 60 uL) stably infected with miRNA were injected subcutaneously in the right flank of the mice, whereas control U87 cells infected with empty vector were injected in the left flank."
item483 .
item484 The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 uL of PBS was performed to establish intravenous (tail vein) leukemia.
item485 The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 uL of PBS was performed to establish intravenous (tail vein) leukemia.
item486 .
item487 .
item488 .
item489 .
item490 .
item491 .
item492 .
item493 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
item494 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
item495 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
item496 .
item497 .
item498 .
item499 .
item500 .
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item502 .
item503 .
item504 .
item505 .
item506 .
item507 .
item508 .
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item510 .
item511 "Male mice were subcutaneously injected with tumour cells near the limbs to establish xenografts (1 × 106/mouse, 0.2 mL for each injection site; METTL3-overexpressing TCam-2/CDDP cells were inoculated once at the initial time and IGF2BP1-inhibited TCam-2/CDDP cells were inoculated every 3 days)."
item512 "Male mice were subcutaneously injected with tumour cells near the limbs to establish xenografts (1 × 106/mouse, 0.2 mL for each injection site; METTL3-overexpressing TCam-2/CDDP cells were inoculated once at the initial time and IGF2BP1-inhibited TCam-2/CDDP cells were inoculated every 3 days)."
item513 .
item514 .
item515 .
item516 .
item517 "A method of randomisation was used to determine the experimental groups. In total, 78 female BALB/c nu/nu mice (4-6-week-old) were selected at random and were divided into different groups. A total of 5 × 106 ISK cells or 1 × 104 ECSCisk were suspended in 100 uL of PBS and then were injected into the mice. After 2 weeks, the presence of tumours was examined. ISK cells (5 × 106, 5 × 105, 5 × 104, 1 × 104, or 1 × 103) and ECSCisk (1 × 104, 1 × 103, 1 × 102, 10, or 1) were injected and analysed for their abilities to form xenograft tumours. After 4 weeks, subsequent experiments were performed."
item518 "To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times."
item519 .
item520 .
item521 "Rats were anesthetized and incised through the midline of the abdomen, and the left renal vertebral arch and arteries were blocked for 45 min, thereby resulting in left kidney ischemia. At the same time, the right kidney was removed, further aggravating the degree of left kidney injury."
item522 "Rats were anesthetized and incised through the midline of the abdomen, and the left renal vertebral arch and arteries were blocked for 45 min, thereby resulting in left kidney ischemia. At the same time, the right kidney was removed, further aggravating the degree of left kidney injury."
item523 "Rats were anesthetized and incised through the midline of the abdomen, and the left renal vertebral arch and arteries were blocked for 45 min, thereby resulting in left kidney ischemia. At the same time, the right kidney was removed, further aggravating the degree of left kidney injury."
item524 .
item525 .
item526 .
item527 .
item528 .
item529 "The specified number of viable SKOV3/DDP cells and SKOV3/DDP cells with TRIM29 knock down were resuspended in 100 uL PBS, injected subcutaneously under the left and right back of 4-week old nude mice respectively (n = 3 per group)."
item530 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
item531 The GC cell line MKN-45 stably infected with lentivirus expressing sh-HBXIP was prepared into 5 × 107 cells/mL cell suspension.
item532 .
item533 .
item534 .
item535 .
item536 "Under anesthesia with ether, the nude mice were disinfected and subcutaneously inoculated with cells transfected with oe-NC, oe-KDM5A + oe-NC and oe-KDM5A + oe-MOB3B at a density of 1 × 106 cells/mouse (200 uL) at the back of the right hind leg."
item537 .
item538 .
item539 Behavioral tests and staining were performed to evaluate the damage to the diabetic hippocampus in model rats.
item540 .
item541 Inducing hepatic fibrosis in C57/BL6 mice by intraperitoneal injection of CCl4. The reversal model of hepatic fibrosis was established by stopping drug after continuous injection of CCl4. Dynamic m6A methylation was evaluated using MeRIP-Seq in the progression and reversal of hepatic fibrosis at different stages.
item542 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item543 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item544 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item545 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item546 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item547 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item548 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item549 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item550 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item551 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item552 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item553 NSCLC gefitinib-resistant cells (5 × 106 cells in 100 uL PBS) were injected subcutaneously into the lateral surface of the left abdomen of 6-week-old female BALB/c nude mice (at least five mice per group to ensure accuracy).
item554 "RC cells SW480 at logarithmic growth phase were prepared into cell suspension with a concentration of about 1 × 107/100 L, which was then injected into the left axilla of nude mice with a 1 ml syringe to establish a subcutaneous mouse xenograft model. Once the tumor volume reached about 50 mm3, the nude mice were injected with miR-96 antagomir or NC antagomir (10 nmol once every 5 days for 5 weeks). After 5 weeks, the mice were euthanized, after which the subcutaneous transplanted tumor was removed, and weighed."
item555 "RC cells SW480 at logarithmic growth phase were prepared into cell suspension with a concentration of about 1 × 107/100 L, which was then injected into the left axilla of nude mice with a 1 ml syringe to establish a subcutaneous mouse xenograft model. Once the tumor volume reached about 50 mm3, the nude mice were injected with miR-96 antagomir or NC antagomir (10 nmol once every 5 days for 5 weeks). After 5 weeks, the mice were euthanized, after which the subcutaneous transplanted tumor was removed, and weighed."
item556 In vivo myocardial I/R injury was performed by 60 min of ligation of LAD followed by 5 h of reperfusion.
item557 In vivo myocardial I/R injury was performed by 60 min of ligation of LAD followed by 5 h of reperfusion.
item558 .
item559 "The first CDX generation was constructed in 4-6 week-old male BALB/c nude mice and treated with sorafenib (30 mg/kg daily, oral gavage). Twelve weeks later, the most resistant xenograft was disaggregated and implanted subcutaneously into 4-6 week-old BALB/c nude mice as the second SR-CDX. Four weeks after implantation, the second SR-CDX mice were treated with sorafenib (30 mg/kg daily, oral gavage) and locally injected with sh-circRNA-SORE lentivirus or its negative control (twice a week for 2 weeks)."
item560 Two hundred milliliters of A549 cells (1 × 106) were injected into the left flank of the back of each mouse.
item561 Two hundred milliliters of A549 cells (1 × 106) were injected into the left flank of the back of each mouse.
item562 Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
item563 "Thirty-two 6 week-old female nude mice divided into four groups (n = 8 per group) for injections of H1299 cells transfected with (a) pLKO.1 + pWPI, (b) shcircNOTCH1 + pWPI, (c) pLKO.1 + oeGPER and (d) shcircNOTCH1 + oeGPER."
item564 .
item565 Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rγ-null (NSG) mice.
item566 Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rγ-null (NSG) mice.
item567 .
item568 .
item569 .
item570 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item571 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item572 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item573 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item574 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item575 "This study investigated the N6-methyladenosine (m6A) methylome of kidneys from three mouse groups: C57 mice (controls), those with CI-AKI (injury group, IG), and those pretreated with berberine (treatment group, TG)."
item576 .
item577 .
item578 .
item579 .
item580 .
item581 .
item582 .
item583 .
item584 .
item585 "When xenografted tumor growth reached 500 mm3, the mice were subjected to intratumoral injection of Ad-con or Ad-HNF3γ every other day. For the PDX model, fresh patient HCC tissues were cut into fragments with a volume of 3 × 3 mm3 and then implanted subcutaneously into the flanks of nude mice. The mice were given sorafenib (30 mg/kg) or vehicle orally twice a week for 24 days."
item586 .
item587 .
item588 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
item589 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
item590 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
item591 .
item592 .
item593 .
item594 .
item595 .
item596 .
item597 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item598 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item599 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item600 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item601 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item602 Athymic BALB/c mice were injected with LCSC cells at the armpit area subcutaneously. The mice were then sacrificed and the tumors recovered.
item603 The in vivo experiment method for transplantation of tumors was subcutaneous injection of 1 × 107 ALKBH5-stable knockdown C918 cells into BALB/c nude mice.
item604 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
item605 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
item606 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
item607 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
item608 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
item609 "A549 cells were transfected with the pZW1-FCS-circNDUFB2 plasmid or pZW1-FCS-Vector plasmid, and selected with G418 (800 ug/ml) for 4 weeks, and then 2 × 106 A549 cells were subcutaneously injected into the right flank of each mouse."
item610 "A549 cells were transfected with the pZW1-FCS-circNDUFB2 plasmid or pZW1-FCS-Vector plasmid, and selected with G418 (800 ug/ml) for 4 weeks, and then 2 × 106 A549 cells were subcutaneously injected into the right flank of each mouse."
item611 "A549 cells were transfected with the pZW1-FCS-circNDUFB2 plasmid or pZW1-FCS-Vector plasmid, and selected with G418 (800 ug/ml) for 4 weeks, and then 2 × 106 A549 cells were subcutaneously injected into the right flank of each mouse."
item612 .
item613 Exosc10cKO_Emx1-Cre mouse embryos were subjected to the p53-inhibitor Pifithrin-Alpha (PFT-Alpha) by intraperitoneal injection of 2.2 mg/kg PFT-Alpha (Selleckchem) into the pregnant mother at between E9.5 and E12.5 or between E9.5 and E15.5. Embryonic brains were isolated at E13.5 or E18.5 and immunohistochemistry was performed.
item614 "The enriched mammosphere cells derived from engineered BT549 and Hs578T with silenced lncRNA KB-1980E6.3 (shKB/vector), BT549, and Hs578T with lncRNA KB-1980E6.3 knockdown combined with ectopic c-Myc (shKB/c-Myc), BT549, and Hs578T with silenced IGF2BP1 (shIGF2BP1/vector), BT549, and Hs578T with knocked down IGF2BP1 combined with ectopic c-Myc (shIGF2BP1/c-Myc), and BT549, and Hs578T/shNC/vector control cells were used in Xenograft experiments. Three doses (1 × 105, 1 × 104 and 1 × 103) of spheres derived from the engineered Hs578T and 1 × 105 of spheres derived from the engineered BT549 were subcutaneously inoculated into 4- to 6-week-old female nude mice (n = 5 per group). Mice were then treated with either bevacizumab (10 mg/kg every 3 days) to form a hypoxic tumor microenvironment or vehicle PBS to form a non-hypoxic condition."
item615 "The enriched mammosphere cells derived from engineered BT549 and Hs578T with silenced lncRNA KB-1980E6.3 (shKB/vector), BT549, and Hs578T with lncRNA KB-1980E6.3 knockdown combined with ectopic c-Myc (shKB/c-Myc), BT549, and Hs578T with silenced IGF2BP1 (shIGF2BP1/vector), BT549, and Hs578T with knocked down IGF2BP1 combined with ectopic c-Myc (shIGF2BP1/c-Myc), and BT549, and Hs578T/shNC/vector control cells were used in Xenograft experiments. Three doses (1 × 105, 1 × 104 and 1 × 103) of spheres derived from the engineered Hs578T and 1 × 105 of spheres derived from the engineered BT549 were subcutaneously inoculated into 4- to 6-week-old female nude mice (n = 5 per group). Mice were then treated with either bevacizumab (10 mg/kg every 3 days) to form a hypoxic tumor microenvironment or vehicle PBS to form a non-hypoxic condition."
item616 .
item617 "A total of 24 male mice were randomly allocated to LFD (low-fat diet), LFDR (low-fat diet + resveratrol), HFD (high-fat diet), and HFDR (high-fat diet + resveratrol) groups for 12 weeks (n = 6/group)."
item618 "A total of 24 male mice were randomly allocated to LFD (low-fat diet), LFDR (low-fat diet + resveratrol), HFD (high-fat diet), and HFDR (high-fat diet + resveratrol) groups for 12 weeks (n = 6/group)."
item619 "A total of 24 male mice were randomly allocated to LFD (low-fat diet), LFDR (low-fat diet + resveratrol), HFD (high-fat diet), and HFDR (high-fat diet + resveratrol) groups for 12 weeks (n = 6/group)."
item620 "A total of 24 male mice were randomly allocated to LFD (low-fat diet), LFDR (low-fat diet + resveratrol), HFD (high-fat diet), and HFDR (high-fat diet + resveratrol) groups for 12 weeks (n = 6/group)."
item621 "A total of 24 male mice were randomly allocated to LFD (low-fat diet), LFDR (low-fat diet + resveratrol), HFD (high-fat diet), and HFDR (high-fat diet + resveratrol) groups for 12 weeks (n = 6/group)."
item622 .
item623 .
item624 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
item625 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
item626 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
item627 MCF-7 cells transfected with sh-LINC00958 or empty vector were resuspended at 2 × 107 cells/mL.
item628 The mice were housed under standardized conditions and received normal diet. The mice were randomly divided into 5 groups (n = 6/each group): (1) Control group: normal mice served as control; (2) TAC-HF group: mice were subjected to transverse aortic constriction (TAC) to establish HF mouse model; (3) Sham group: sham-operated mice were subjected the same surgery without the placement of a ligature; (4) Doxorubicin-HF group: mice were intraperitoneally injected with doxorubicin to construct HF mouse model; and (5) PBS: mice were intraperitoneally injected with equal PBS as control.
item629 .
item630 .
item631 .
item632 .
item633 .
item634 Used 1 × 106 SNHG3 knocked down KY-SE150 cells and NC lentivirus to inject into the right flank of mice to generate xenografts.
item635 Used 1 × 106 SNHG3 knocked down KY-SE150 cells and NC lentivirus to inject into the right flank of mice to generate xenografts.
item636 HCC cells (1 × 106/100 uL PBS) were administered to 4-week-old female BALB/c nude mice by subcutaneous injection (n = 6).
item637 HCC cells (1 × 106/100 uL PBS) were administered to 4-week-old female BALB/c nude mice by subcutaneous injection (n = 6).
item638 "For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells."
item639 "For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells."
item640 "For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells."
item641 "For tumor growth studies, whether in vivo RBM15 knockdown/overexpression experiments or in vivo rescue experiments, each group included six mice. Each mouse was injected with 100 uL of lentivirus-transfected tumor cells."
item642 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item643 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item644 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item645 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item646 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item647 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
item648 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
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item661 "The rats were adaptively fed for 1 week and then subcutaneous injection of complete Freund's adjuvant into the left hindfoot toe to establish an adjuvant arthritis (AA) rat model. After induction of the immune response for 20 days, all rats were anesthetized by the intraperitoneal injection of 1% sodium pentobarbital (60 mg/kg) and sacrificed by exsanguination of the abdominal aorta."
item662 "The rats were adaptively fed for 1 week and then subcutaneous injection of complete Freund's adjuvant into the left hindfoot toe to establish an adjuvant arthritis (AA) rat model. After induction of the immune response for 20 days, all rats were anesthetized by the intraperitoneal injection of 1% sodium pentobarbital (60 mg/kg) and sacrificed by exsanguination of the abdominal aorta."
item663 "The rats were adaptively fed for 1 week and then subcutaneous injection of complete Freund's adjuvant into the left hindfoot toe to establish an adjuvant arthritis (AA) rat model. After induction of the immune response for 20 days, all rats were anesthetized by the intraperitoneal injection of 1% sodium pentobarbital (60 mg/kg) and sacrificed by exsanguination of the abdominal aorta."
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item668 The mice were randomly divided into groups before subcutaneous injection with cells (107) suspended in 200 uL of phosphate-buffered saline. Tumors were measured on day 7 and then once every 7 days until 28 days after injection.
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item691 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of six-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck millipore, 217 503), then the C2C12 cells, which stably overexpressed METTL3 or GFP control, were injected to the injury site of mice on the following day. The regenerated muscles were collected at day 7 post-injection, frozen in liquid nitrogen for RNA and protein extraction."
item692 "10 rats were divided into control and HPH group. In detail, 5 rats of the hypoxia groups were exposed to hypoxia (10%O2) chamber (AiPu XBS-02B, China) for 4 weeks. In addition, 5 rats of control group were kept under normoxic conditions (21% O2) for 4 weeks. Rats were housed in standard polypropylene cages under controlled photocycle (12 h light/12 h dark) under 22-25 ℃ temperature."
item693 "10 rats were divided into control and HPH group. In detail, 5 rats of the hypoxia groups were exposed to hypoxia (10%O2) chamber (AiPu XBS-02B, China) for 4 weeks. In addition, 5 rats of control group were kept under normoxic conditions (21% O2) for 4 weeks. Rats were housed in standard polypropylene cages under controlled photocycle (12 h light/12 h dark) under 22-25 ℃ temperature."
item694 .
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item696 .
item697 "In the control mice or ADR mice group, the parental or chemo-resistant K562 cells were infected with LV-shCtrl. In the ADR + shLINC00470 group, the chemo-resistant K562 cells were infected with LV- shLINC00470. These cells were injected, respectively, into these 5-week-old mice subcutaneously."
item698 "In the control mice or ADR mice group, the parental or chemo-resistant K562 cells were infected with LV-shCtrl. In the ADR + shLINC00470 group, the chemo-resistant K562 cells were infected with LV- shLINC00470. These cells were injected, respectively, into these 5-week-old mice subcutaneously."
item699 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
item700 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
item701 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
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item711 BGC-823 cells (5 × 106) with shRNAs targeting YTHDF1 (sh-YTHDF1) or shRNAs targeting control (sh-NC) were trypsinized and suspended in 0.1 mL PBS and injected subcutaneously into the BALB/c mice (n = 5 mice per group).
item712 .
item713 .
item714 Balb/c female nude mice at 4-6 weeks old were injected with 4 × 106 cells subcutaneously on the back.
item715 "HCC cells (5 × 106 cells/mouse) that had been transfected with oe-NC + sh-NC, oe-KDM5B + sh-NC, or oe-KDM5B + sh-ITGA6, or treated with NS, GSK-467 (a selective inhibitor of KDM5B) + oe-NC or GSK-467 + oe-ITGA6 were then subcutaneously implanted into the back of mice."
item716 .
item717 "As cells (5 million) in Matrigel or As-T (1 million) cells in PBS with or without gene manipulations were injected subcutaneously into the right flanks of female mice (6-8 weeks of age). For treatment with CS1 or CS2, As-T cells (1 million) in PBS were injected subcutaneously into the right flanks of 6-week-old female nude mice."
item718 "As cells (5 million) in Matrigel or As-T (1 million) cells in PBS with or without gene manipulations were injected subcutaneously into the right flanks of female mice (6-8 weeks of age). For treatment with CS1 or CS2, As-T cells (1 million) in PBS were injected subcutaneously into the right flanks of 6-week-old female nude mice."
item719 "As cells (5 million) in Matrigel or As-T (1 million) cells in PBS with or without gene manipulations were injected subcutaneously into the right flanks of female mice (6-8 weeks of age). For treatment with CS1 or CS2, As-T cells (1 million) in PBS were injected subcutaneously into the right flanks of 6-week-old female nude mice."
item720 "As cells (5 million) in Matrigel or As-T (1 million) cells in PBS with or without gene manipulations were injected subcutaneously into the right flanks of female mice (6-8 weeks of age). For treatment with CS1 or CS2, As-T cells (1 million) in PBS were injected subcutaneously into the right flanks of 6-week-old female nude mice."
item721 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
item722 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
item723 "Twelve female BALB/c nude mice (aged 4 weeks, 18-22g) were randomly divided into 2 groups. Stable circMETTL3-expression SUM1315 cells or control cells (1×106 cells in 0.1 mL PBS) was subcutaneously injected into mammary fat pads of the mice and the growth of tumors was followed up every week. Tumor volume was measured every week using a caliper, calculated as (length × width2)/2. After 4 weeks, mice were sacrificed and checked for final tumor weight."
item724 "Twelve female BALB/c nude mice (aged 4 weeks, 18-22g) were randomly divided into 2 groups. Stable circMETTL3-expression SUM1315 cells or control cells (1×106 cells in 0.1 mL PBS) was subcutaneously injected into mammary fat pads of the mice and the growth of tumors was followed up every week. Tumor volume was measured every week using a caliper, calculated as (length × width2)/2. After 4 weeks, mice were sacrificed and checked for final tumor weight."
item725 "Upon the development of leukemic disease (established using a white blood cell (WBC) count), 0.1 mg/kg or 0.5 mg/kg of SsD was intraperitoneally injected three times per week for three consecutive weeks."
item726 "The 40 nude mice of each large group were classified into 8 small groups (n = 5) and subcutaneously injected with transfected cell suspension in the back (5 × 106 cells/mL/mouse). The length and width of tumors were recorded every 4 days, and the tumor volume = (length × width2)/2. The tumor growth curve was thereby graphed. On the 28th day of injection, mice were euthanized by neck dislocation, and the xenografts were harvested, photographed, and weighed."
item727 "The 40 nude mice of each large group were classified into 8 small groups (n = 5) and subcutaneously injected with transfected cell suspension in the back (5 × 106 cells/mL/mouse). The length and width of tumors were recorded every 4 days, and the tumor volume = (length × width2)/2. The tumor growth curve was thereby graphed. On the 28th day of injection, mice were euthanized by neck dislocation, and the xenografts were harvested, photographed, and weighed."
item728 Hepatocyte-specific knockout USP48 was obtained by crossing Alb-Cre mice with USP48flox/flox mice.
item729 Hepatocyte-specific knockout USP48 was obtained by crossing Alb-Cre mice with USP48flox/flox mice.
item730 .
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item732 .
item733 "U87 cells (5 × 105) transfected with an empty vector, METTL3 shRNA, or METTL3 overexpression vector were inoculated into the right frontal node of nude mice."
item734 "The mice were maintained in cages under standard environmental conditions (temperature 25 ± 2 ℃, humidity 55 ± 5% and 12-h light/12-h dark cycle) and given free access to food and tap water. All mice were randomly divided into three groups with 6 in each group. To establish xenograft model, A549 cells (1 × 107) transfected with si-control (si-NC), si-ALKBH5 or si-ALKBH5 + RMRP were injected subcutaneously into a single side of the armpit of each mouse."
item735 HCT8/T xenografts derived from shNC or shIGF2BP3-1 HCT8/T cells were established through subcutaneous inoculation of cells (6×106) into nude mice.
item736 .
item737 .
item738 U251/TR cells (2 × 106 per mouse) with stable transfection of sh-circ_0072083 or sh-NC were subcutaneously injected into mice.
item739 .
item740 .
item741 .
item742 .
item743 "For the ROS-induced DNA damage analysis, the indicated cell lines were treated with or without 100 uM hydrogen peroxide (H2O2), or 80 uM Carbonyl cyanide m-chlorophenylhydrazone (CCCP) for 6 hours. For the in vivo ROS study, DMSO and 5 mg/kg CCCP was intraperitoneally injected in to three pairs of mice."
item744 "For the ROS-induced DNA damage analysis, the indicated cell lines were treated with or without 100 uM hydrogen peroxide (H2O2), or 80 uM Carbonyl cyanide m-chlorophenylhydrazone (CCCP) for 6 hours. For the in vivo ROS study, DMSO and 5 mg/kg CCCP was intraperitoneally injected in to three pairs of mice."
item745 .
item746 .
item747 "The mice were housed with filtered air, 12 h light/dark cycle, constant temperature (25℃), relative humidity (50±5%) and free access to food and water. In order to establish a human NSCLC xenograft model, 5×106 H1299, sh-METTL3-H1299 and METTL3 stably overexpressed H1299 cells (2×106 per mouse) were subcutaneously injected into mice. Tumor growth was observed daily. Tumor volume was calculated as follows: 0.5× (length × width2). At 24 days post-inoculation, the maximum diameter exhibited by a single subcutaneous tumor was 15 mm and mice were anesthetized by intraperitoneal administration of sodium pentobarbital (50 mg/kg), then sacrificed by cervical dislocation."
item748 .
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item751 "Adult male C57BL/6J mice (weighting 18-25 g) were obtained from Laboratory Animal Center, Zhengzhou University. Mice were subcutaneously injected with 1 × 107 CEM-C1 cells for tumorigenesis and randomly divided into four group: control group; control + Dex; sh-RNA + Dex; sh-USP1 + Dex. Mice treatment with Dex were intraperitoneally injected with 8 mg/kg Dex every day for 10 consecutive days after tumor growth and mice treatment with sh-RNA or sh-USP1 were injected intravenously with 2 mg/Kg sh-RNA or USP1 sh-RNA. The control group of mice were injected with the same volume of normal saline. After the treatment of each group, the mice were housed and fed in a room with an ambient temperature of 25℃, and the survival time, weight of the mice, and tumor weight were recorded. When rats were sacrificed, tissues were harvested for Western blot analysis."
item752 "Adult male C57BL/6J mice (weighting 18-25 g) were obtained from Laboratory Animal Center, Zhengzhou University. Mice were subcutaneously injected with 1 × 107 CEM-C1 cells for tumorigenesis and randomly divided into four group: control group; control + Dex; sh-RNA + Dex; sh-USP1 + Dex. Mice treatment with Dex were intraperitoneally injected with 8 mg/kg Dex every day for 10 consecutive days after tumor growth and mice treatment with sh-RNA or sh-USP1 were injected intravenously with 2 mg/Kg sh-RNA or USP1 sh-RNA. The control group of mice were injected with the same volume of normal saline. After the treatment of each group, the mice were housed and fed in a room with an ambient temperature of 25℃, and the survival time, weight of the mice, and tumor weight were recorded. When rats were sacrificed, tissues were harvested for Western blot analysis."
item753 "Mice were provided adlibitum with water and a standard laboratory chow diet. The animal room was held a standard temperature, humidity and illumination. After intraperitoneal injection of FTO overexpression vector and NR to mice for 7 days, inguinal white adipose tissue (iWAT) were collected from mice."
item754 "Mice were provided adlibitum with water and a standard laboratory chow diet. The animal room was held a standard temperature, humidity and illumination. After intraperitoneal injection of FTO overexpression vector and NR to mice for 7 days, inguinal white adipose tissue (iWAT) were collected from mice."
item755 To induce recombination at 8 weeks of age both CAG-CreERT;Ythdf2fl/fl and Ythdf2fl/fl littermates were injected with 75mg/kg body weight tamoxifen dissolved in corn oil daily for 5 days.
item756 Neonatal mouse cardiomyocytes (CMs) were blinded to isolate from 7-10 cases of postnatal 1-day old wildtype C57/B mice and cultured using Pierce Primary Cardi Page 10.omyocyte Isolation Kit (Thermo Fisher Scientific) as the manufacturer's instructions.
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item769 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 6/group). Calu1 cells (4 × 106) that had been stably transfected with sh-LCAT3 or scramble were implanted subcutaneously into the nude mice. Tumor growth was measured after one week, and tumor volumes were calculated with the following formula: Volume (cm3) = (length × width2)/2. After four weeks, the mice were euthanized, and the tumors were collected and weighed. For the in vivo tumor invasion assay, 1.2 × 106 scramble or shLCAT3 cells were injected intravenously into the tail vein of nude mice (n = 6/group). 1.5 mg luciferin (Gold Biotech, St Louis, MO, USA) was administered once a week for 4 weeks, to monitor metastases using an IVIS@ Lumina II system (Caliper Life Sciences, Hopkinton, MA, USA)."
item770 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 6/group). Calu1 cells (4 × 106) that had been stably transfected with sh-LCAT3 or scramble were implanted subcutaneously into the nude mice. Tumor growth was measured after one week, and tumor volumes were calculated with the following formula: Volume (cm3) = (length × width2)/2. After four weeks, the mice were euthanized, and the tumors were collected and weighed. For the in vivo tumor invasion assay, 1.2 × 106 scramble or shLCAT3 cells were injected intravenously into the tail vein of nude mice (n = 6/group). 1.5 mg luciferin (Gold Biotech, St Louis, MO, USA) was administered once a week for 4 weeks, to monitor metastases using an IVIS@ Lumina II system (Caliper Life Sciences, Hopkinton, MA, USA)."
item771 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 6/group). Calu1 cells (4 × 106) that had been stably transfected with sh-LCAT3 or scramble were implanted subcutaneously into the nude mice. Tumor growth was measured after one week, and tumor volumes were calculated with the following formula: Volume (cm3) = (length × width2)/2. After four weeks, the mice were euthanized, and the tumors were collected and weighed. For the in vivo tumor invasion assay, 1.2 × 106 scramble or shLCAT3 cells were injected intravenously into the tail vein of nude mice (n = 6/group). 1.5 mg luciferin (Gold Biotech, St Louis, MO, USA) was administered once a week for 4 weeks, to monitor metastases using an IVIS@ Lumina II system (Caliper Life Sciences, Hopkinton, MA, USA)."
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item779 "Subcutaneously injected with 5 × 106 Jurkat cells and fed under Specific Pathogen Free (SPF) conditions. One week later, tumor mass was injected once a week with Agomir NC, Agomir-211, Antagomir NC and Antagomir 211, respectively, for three weeks."
item780 .
item781 "IGF2BP2 activates the expression of ErbB2 by recognizing the m6A of YAP, thus affecting the cell cycle of CRC, inhibiting cell apoptosis, and promoting proliferation."
item782 "IGF2BP2 activates the expression of ErbB2 by recognizing the m6A of YAP, thus affecting the cell cycle of CRC, inhibiting cell apoptosis, and promoting proliferation."
item783 .
item784 .
item785 "Subcutaneously injected shMETTL3 or shNC-expressing U87-MG-TMZ cells into BALB/c NOD mice. After confirmation of GBM implantation, mice were treated with TMZ (66 mg/kg/d, 5 d per week, for 3 cycles)."
item786 "Subcutaneously injected shMETTL3 or shNC-expressing U87-MG-TMZ cells into BALB/c NOD mice. After confirmation of GBM implantation, mice were treated with TMZ (66 mg/kg/d, 5 d per week, for 3 cycles)."
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item817 "For the PDX model, fresh patient HCC tissues were cut into fragments with a volume of 3 × 3 mm3 and then implanted subcutaneously into the flanks of nude mice. The mice were given sorafenib (30 mg/kg) or vehicle orally twice a week for 24 days. This procedure was approved by the Ethics Committee of Jinling Hospital."
item818 .
item819 CircARHGAP12-stable knockdown cervical cancer cells (100 uL PBS containing 5 × 106 cells) were subcutaneously injected into the lateral flank of BALB/c nude mice.
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item830 "All mice were housed under a 12 h light/dark cycle with constant temperature about 25 ℃ and relative humidity approximating 55 %. The mice had free access to food and water for 10 days prior to the experiment. Forty mice were randomly selected and divided into four groups of 10 mice each. After 10 days, mice received an intraperitoneal injection of 22 mg / mL sodium pentobarbital (diluted in saline) followed by 167 uM LPS (60 uL) Saline solution was instilled into the oral cavity through the posterior pharyngeal wall. Pinch the nares quickly and hold for 30 s, model is successful when all fluid is absorbed into the nasal cavity, and slight tracheal rales appear. Lentiviral vectors containing pcDNA-SNHG4 (150 uM) or pcDNA-3.1 were intratracheally injected into mice. Twenty-one days after establishing the model, mice were intraperitoneally injected with 3% sodium pentobarbital and euthanized by overdose anesthesia at a dose of 90 mL/Kg, and organs and tissues were removed for follow-up studies."
item831 "All mice were housed under a 12 h light/dark cycle with constant temperature about 25 ℃ and relative humidity approximating 55 %. The mice had free access to food and water for 10 days prior to the experiment. Forty mice were randomly selected and divided into four groups of 10 mice each. After 10 days, mice received an intraperitoneal injection of 22 mg / mL sodium pentobarbital (diluted in saline) followed by 167 uM LPS (60 uL) Saline solution was instilled into the oral cavity through the posterior pharyngeal wall. Pinch the nares quickly and hold for 30 s, model is successful when all fluid is absorbed into the nasal cavity, and slight tracheal rales appear. Lentiviral vectors containing pcDNA-SNHG4 (150 uM) or pcDNA-3.1 were intratracheally injected into mice. Twenty-one days after establishing the model, mice were intraperitoneally injected with 3% sodium pentobarbital and euthanized by overdose anesthesia at a dose of 90 mL/Kg, and organs and tissues were removed for follow-up studies."
item832 .
item833 "Osmotic mini-pumps containing AngII (1 ug/kg/min, Enzo Bioche) were implanted in 7-week-old male mice. To interfere with the expression of KIAA1429, ALKBH5, or DDX6 in vivo, adeno-associated virus 9 (AAV9) vectors carrying a variety of overexpression plasmids or interfering RNA were randomly injected through the tail vein to C57BL/6N mice."
item834 "Osmotic mini-pumps containing AngII (1 ug/kg/min, Enzo Bioche) were implanted in 7-week-old male mice. To interfere with the expression of KIAA1429, ALKBH5, or DDX6 in vivo, adeno-associated virus 9 (AAV9) vectors carrying a variety of overexpression plasmids or interfering RNA were randomly injected through the tail vein to C57BL/6N mice."
item835 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
item836 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
item837 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
item838 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
item839 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
item840 "After adaptive feeding for 1 week, db/db mice were randomly divided into five groups (n = 6): db/db group, db/db + rAAV group, db/db + rAAV-METTL14 group, db/db + rAAV-klotho group, and db/db + rAAV-METTL14 + rAAV-klotho group. Except db/db group, the other four groups were injected with recombinant adeno-associated virus (rAAV) control, rAAV mediated delivery of METTL14 (rAAV-METTL14), or/and rAAV mediated delivery of klotho (rAAV-klotho) respectively via tail vein. Six db/m mice were chosen as the normal control."
item841 .
item842 "To establish a tumour model, C57BL/6 mice were intraperitoneal injected with 25 mg/kg diethylnitrosamine at 2 weeks of age."
item843 "To establish a tumour model, C57BL/6 mice were intraperitoneal injected with 25 mg/kg diethylnitrosamine at 2 weeks of age."
item844 "To establish a tumour model, C57BL/6 mice were intraperitoneal injected with 25 mg/kg diethylnitrosamine at 2 weeks of age."
item845 "Sixteen female goats in good body condition and suitable for pregnancy were selected. All selected goats underwent estrus synchronization treatment and were naturally mated. After 75 days of gestation, four male fetuses were removed from five pregnant goats during abortion operations, and their longissimus muscle samples were collected."
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item857 "The growth of CC cells in vivo was examined by subcutaneous injection of LoVo cells or Caco-2 cells into NSG mice, while the metastatic ability of cells in vivo by intracardiac injection into mice."
item858 "The number of cells inoculated in each mouse was 4 × 106, 1 × 106, 2 × 106 and 1 × 106, respectively."
item859 "The number of cells inoculated in each mouse was 4 × 106, 1 × 106, 2 × 106 and 1 × 106, respectively."
item860 "The number of cells inoculated in each mouse was 4 × 106, 1 × 106, 2 × 106 and 1 × 106, respectively."
item861 "All mice were housed in specific pathogen-free conditions in the animal facility with constant temperature and humidity under a 12-h light/12-h dark cycle, with free access to water and food. After a week of adaptation, they were then divided into two groups randomly and fed with a HFD (60 kcal% fat, D12492, Research Diets) or standard normal chow diet (CD) for 16 weeks, respectively."
item862 "All mice were housed in specific pathogen-free conditions in the animal facility with constant temperature and humidity under a 12-h light/12-h dark cycle, with free access to water and food. After a week of adaptation, they were then divided into two groups randomly and fed with a HFD (60 kcal% fat, D12492, Research Diets) or standard normal chow diet (CD) for 16 weeks, respectively."
item863 HCC-LM3 cells with silenced LNCAROD or the negative control were subcutaneously injected into the flank region of the mice at 2 × 106 cells.
item864 .
item865 "The 60 female Kunming mice were divided into two groups (n = 30): control group (injection of physiological saline through the caudal vein) and colistin group (injection of 15 mg/kg colistin, twice a day, with an eight-hour interval)."
item866 "The 60 female Kunming mice were divided into two groups (n = 30): control group (injection of physiological saline through the caudal vein) and colistin group (injection of 15 mg/kg colistin, twice a day, with an eight-hour interval)."
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item870 .
item871 "To establish mice model with ADR nephropathy, adult male C57BL/6J mice (8-12 weeks of age) were purchased from Animal Center of Fudan University and injected with 19.5 mg/kg ADR (D1515, Sigma-Aldrich, St-Louis, MO, USA) intravenously via tail vein."
item872 "Used to inject 10 uL of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection."
item873 A total 5 × 106 stably transfected SiHa cells were subcutaneously injected into the flank of nude mice.
item874 .
item875 "M3LKO (Mettl3fl/fl; Alb-Cre) mice were generated by crossing Mettl3fl/fl mice (provided by Dr. Jacob Hanna) with albumin-Cre mice (Jackson Laboratories, Bar Harbor, ME), and genotypes were confirmed by tail-DNA PCR using primers as previously described."
item876 "M3LKO (Mettl3fl/fl; Alb-Cre) mice were generated by crossing Mettl3fl/fl mice (provided by Dr. Jacob Hanna) with albumin-Cre mice (Jackson Laboratories, Bar Harbor, ME), and genotypes were confirmed by tail-DNA PCR using primers as previously described."
item877 .
item878 .
item879 .
item880 "VA-Lip-Mettl4-shRNA, VA-Lip-Fto-Plasmid and VA-Lip-Ythdf1-shRNA (0.75 mg/kg) were injected intravenously 3 times a week."
item881 .
item882 "LUAD cells stably HCG11 and/or LATS1 overexpressed or silenced were subcutaneously injected into the flank of the BALB/c nude mice (male, 4 weeks old)."
item883 "LUAD cells stably HCG11 and/or LATS1 overexpressed or silenced were subcutaneously injected into the flank of the BALB/c nude mice (male, 4 weeks old)."
item884 .
item885 "Four-week-old male BALB/c nude mice (purchased from Lingchang company) were randomly divided into three groups, each group has five mice. Each of the mice was injected subcutaneously on the right lateral back with 1 × 106 of each lentivirus infected SW480 cells in which hnRNPA2B1 was knocked out or negative control cells. Mice were killed at day 29, and tumors were then isolated, photographed."
item886 "Four-week-old male BALB/c nude mice (purchased from Lingchang company) were randomly divided into three groups, each group has five mice. Each of the mice was injected subcutaneously on the right lateral back with 1 × 106 of each lentivirus infected SW480 cells in which hnRNPA2B1 was knocked out or negative control cells. Mice were killed at day 29, and tumors were then isolated, photographed."
item887 "A498 cells (1 × 106 cells) were resuspended in 100 uL of PBS and subcutaneously injected into the axillary fossa of nude mice (BALB/c-nude, 4 weeks old)."
item888 .
item889 "The WT-si-NC, WT-si-Ythdc1 and WT-si-Sqstm1 groups were intracutaneously injected with corresponding siRNAs (si-NC, si-Ythdc1, or si-Sqstm1, 2.5 nmol) on the circle."
item890 "The WT-si-NC, WT-si-Ythdc1 and WT-si-Sqstm1 groups were intracutaneously injected with corresponding siRNAs (si-NC, si-Ythdc1, or si-Sqstm1, 2.5 nmol) on the circle."
item891 .
item892 "A total of 2×106 tumor cells were suspended in 200 uL of PBS and injected the right flank of nude mice. The tumor sizes were measured weekly as soon as the tumors were measurable, and the tumor volumes were calculated using the following formula: volume (mm3) = width2 (mm2) × length (mm)/2. After 4 weeks, the mice were sacrificed, and the tumors were harvested."
item893 Indicated cells (1 × 107) were subcutaneously injected into 4-week-old male nude mice. Tumor volume was measured every 5 days.
item894 Indicated cells (1 × 107) were subcutaneously injected into 4-week-old male nude mice. Tumor volume was measured every 5 days.
item895 .
item896 The knockout first allele was converted into a conditional allele by crossing Ythdf2 knockout first mice with Flp deleter mice. The resulting floxed Ythdf2 mice were crossed with Vasa-GFPCre knock-in mice to allow specific knockout of Ythdf2 in germ cells.
item897 Male C57BL/6 mice (4-6 weeks old) were used in all tumor allografting experiments and transplanted with GL261-luc cells (1×105) into the frontal lobes of brains.
item898 .
item899 .
item900 .
item901 "All mice described above were maintained on the C57BL/6 J background. Mice lacking Mettl3 in oocytes (referred to as Mettl3Gdf9 cKO) were generated by crossing Mettl3flox/flox mice with Gdf9-Cre mice. The Mettl3flox/flox female mice were used as the control group (referred to as WT). For the fertility test, six pairs of 6 weeks Mettl3flox/flox and Mettl3Gdf9 cKO female mice were randomly selected and continually mated to Mettl3flox/flox male mice which have been confirmed fertility for 5 months. The number of pups and litter size from each female was recorded."
item902 "All mice described above were maintained on the C57BL/6 J background. Mice lacking Mettl3 in oocytes (referred to as Mettl3Gdf9 cKO) were generated by crossing Mettl3flox/flox mice with Gdf9-Cre mice. The Mettl3flox/flox female mice were used as the control group (referred to as WT). For the fertility test, six pairs of 6 weeks Mettl3flox/flox and Mettl3Gdf9 cKO female mice were randomly selected and continually mated to Mettl3flox/flox male mice which have been confirmed fertility for 5 months. The number of pups and litter size from each female was recorded."
item903 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
item904 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
item905 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
item906 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item907 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item908 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item909 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item910 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item911 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
item912 "Mice were anaesthetized with isoflurane supplied in a mouse anaesthesia apparatus, followed with joint surgery on the right joint by sectioning the medial meniscotibial ligament."
item913 "Mice were anaesthetized with isoflurane supplied in a mouse anaesthesia apparatus, followed with joint surgery on the right joint by sectioning the medial meniscotibial ligament."
item914 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item915 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item916 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item917 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item918 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item919 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item920 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item921 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item922 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item923 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item924 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item925 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item926 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item927 "After being anesthetized with urethane (i.p.), SD rats were endotracheally intubated and ventilated using an animal ventilator under the conditions: respiratory rate of 70 breaths/min, tidal volume of 20 ml/kg, and inspiratory/expiratory ratio of 1:1."
item928 .
item929 "Approximately 1 × 107 stably transfected T24 cells were subcutaneously injected into BALB/c nude mice. The length (L) and width (W) of the tumours were measured weekly using callipers, while their volume was calculated using the equation: V = (L × W2)/2. After 4 weeks of injections, the mice were euthanised, and the tumour tissues were removed and weighed."
item930 "Approximately 1 × 107 stably transfected T24 cells were subcutaneously injected into BALB/c nude mice. The length (L) and width (W) of the tumours were measured weekly using callipers, while their volume was calculated using the equation: V = (L × W2)/2. After 4 weeks of injections, the mice were euthanised, and the tumour tissues were removed and weighed."
item931 .
item932 .
item933 .
item934 .
item935 .
item936 .
item937 .
item938 .
item939 .
item940 .
item941 .
item942 .
item943 .
item944 .
item945 .
item946 .
item947 .
item948 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item949 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item950 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item951 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item952 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item953 "The 8-10 weeks old mice were fed either a high fat diet or HF-CDAA , ad lib for 6-12 weeks. Chow diet was used as control for HFD.The mouse liver was perfused with PBS through portal vein, and liver tissue was cut into small pieces by a scissor. The single cell was made using syringe plunger to mull the tissue, and passed through a 40 uM cell strainer."
item954 5 × 105 selected cells were injected via the tail vein into 4- to 5-week-old NCG mice.
item955 .
item956 .
item957 .
item958 .
item959 Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
item960 Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
item961 .
item962 .
item963 .
item964 .
item965 .
item966 .
item967 .
item968 .
item969 "1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group."
item970 "1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group."
item971 .
item972 .
item973 .
item974 .
item975 .
item976 The U87MG cells (1 × 107 cells in 0.1 ml PBS) were injected subcutaneously into BALB/c nude mice. Tumor width and length were recorded every 5 days.
item977 "The mice were maintained on a 12-h light/dark cycle (lights on from 8:00 a.m. to 8:00 p.m.). On day 7.5 of pregnancy (E7.5), ethionine (Sigma-Aldrich, USA) was intraperitoneally injected only once at a dose of 500 mg/kg to establish the NTDs embryo model. And SAM (MedChemExpress, USA) was intraperitoneally injected only once at a dose of 30 mg/kg. The same dose was intraperitoneally injected to the pregnant mice for control group."
item978 "The mice were maintained on a 12-h light/dark cycle (lights on from 8:00 a.m. to 8:00 p.m.). On day 7.5 of pregnancy (E7.5), ethionine (Sigma-Aldrich, USA) was intraperitoneally injected only once at a dose of 500 mg/kg to establish the NTDs embryo model. And SAM (MedChemExpress, USA) was intraperitoneally injected only once at a dose of 30 mg/kg. The same dose was intraperitoneally injected to the pregnant mice for control group."
item979 .
item980 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
item981 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
item982 "Specific pathogen-free (SPF) female NOD/SCID mice (5-6 weeks old) were randomly distributed into two groups: the OECtrl group and the OEMETTL14 groups. Phosphate buffer (200 uL) containing approximately 5 × 107 HSC3 or CAL33 cells was subcutaneously injected into the inner thigh of each mouse. The mice were euthanized two weeks after injection, and the tumour xenografts were harvested, photographed, weighed, and fixed."
item983 "For the subcutaneous implantation model, 5×106 control and CASC11 overexpressing Huh7 cells were suspended in the 100 uL serum-free DMEM medium and then injected subcutaneously. For the metastatic model, 1×105 control and CASC11 overexpressing Huh7 cells were suspended in the 50 uL serum-free DMEM medium, followed by being injected into the tail vein of nude mice."
item984 "For the subcutaneous implantation model, 5×106 control and CASC11 overexpressing Huh7 cells were suspended in the 100 uL serum-free DMEM medium and then injected subcutaneously. For the metastatic model, 1×105 control and CASC11 overexpressing Huh7 cells were suspended in the 50 uL serum-free DMEM medium, followed by being injected into the tail vein of nude mice."
item985 .
item986 "Clone C4 was expanded and electroporated with Cre-expressing plasmid pOG231, followed by culture in media containing 2 uM ganciclovir. Ninety-two clones were screened for removal of the HyTK cassette and presence of loxP sites flanking Ythdc2 exons 13-16, resulting in nine positive clones (Ythdc2fl/+)."
item987 Male Sprague-Dawley rats (375-400 g) liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4) and olive oil (a ratio of 2:3) twice per week.
item988 Mice were inoculated subcutaneously with 5× 106 corresponding cells per flank in 150 uL of 50% Matrigel Matrix (Corning). Tumor diameters were measured semi-weekly with a caliper and tumor volumes calculated using the formula: (width2 × length)/2 = V (mm3).
item989 "NOD.CB17-Prkdcscid/J none castrated SCID male mice 4 weeks old were purchased from Jackson Laboratories. After a one week period of acclimation, the mice were injected with 6×106 TetOn-Flag-ELF3 LNCaP suspended in 200 uL of a 50% mixture containing RPMI 1640 medium and Matrigel matrix basement membrane (BD Corporation, Bedford, MA) subcutaneously into the right flank region. The mice were either fed with doxycycline 200 mg/kg containing diet (BioServ, NJ) to induce the ELF3 expression or with a control regular diet. The sizes of the resultant tumors were measured weekly."
item990 "In the present study, we assessed Smad3 +/- mice for a comparatively long period to examine the effects of Smad3 expression and phosphorylation and to evaluate the involvement of Smad3 in DN. Heterozygous Smad3- knockout mice were kindly provided by Dr. Yasue, University of Tokushima. We attempted to generate Smad3-null mice using pairs of Smad3 mice, but progeny was rarely obtained, and pups were fragile and could not survive for a long period (5 weeks at the most). Therefore, BKS/Cg-m Lepr db (db/db) x Smad3 +/- mice were developed using pairs of Lepr db +/- x Smad3 +/-. Smad3 +/-;db/+ mice were generated by crossing Smad3 +/- and db/+ mice. Moreover, Smad3 +/-;db/db were generated by crossing Smad3 +/-;db/+ and db/+ mice as previously described. Blood glucose concentrations were measured from the tail vein (glucose assay kit; Abbott). The diabetic phenotype was confirmed 4 weeks after birth by blood glucose >300 mg/dl."
item991 .
item992 .
item993 "The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution."
item994 "The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution."
item995 "The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution."
item996 "The male BALB/c nude mice were randomized divide into two groups, each group including six 4 weeks old nude mice. Investigators were blinded to the treatment groups during data collection and subsequent data analysis. In the subcutaneous xenograft model, 5 × 105 cells were subcutaneously injected in the right flanks of nude mice. In the orthotopic intracranial mouse model, each mouse was intracranially injected with 1 × 105 luciferase transfected U87MG cells in 10 uL PBS solution."
item997 .
item998 .
item999 .
item1000 "Severe combined immunodeficiency (SCID) female mice aged 6-8 weeks were transplanted subcutaneously with 2 × 107 P4-pBIG2i-hIGFBP3 or P4-pBIG2i transfectant cells. Tumor growth was measured using Vernier calipers and the volume was calculated using the formula: length × width × width × 0.52, which approximates the volume of an elliptical solid mass. Doxycycline at 2 mg/mL in drinking water with 2% sucrose was used to feed the animals when the tumor size was over 0.5 cm in diameter. The xenograft tumors were removed from five animals from each group on days 4, 7, 11, 14, and 20 after doxycycline treatment."
item1001 "Implanting 5000 human derived GSCs into the right cerebral cortex of NSG mice at a depth of 3.5 mm under a University of California, San Diego Institutional Animal Care and Use Committee (IACUC) approved protocol. Brains were harvested and fixed in 4% formaldehyde, cryopreserved in 30% sucrose, and then cryosectioned. Hematoxylin and eosin (H&E) staining was performed on sections for histological analysis. In parallel survival experiments, mice were observed until the development of neurological signs. For in vivo drug treatment studies, intracranial xenografts were generated by implanting 5000 patient-derived GSCs (387 and 4121) into the right cerebral cortex of NSG mice as described above. Mice recovered for 7 days were randomly assigned into drug vs. treatment group by a blinded investigator. Mice were then treated daily with either vehicle (25 mM Tartaric acid) or 50 mg/kg linsitinib by oral gavage."
item1002 "Paired littermates of F2 (Igfbp3+/+:KrasG12D/+ and Igfbp3-/-:KrasG12D/+) were sacrificed ranging from ages 4 to 7 months. After preliminary analysis of F2 mice, we sacrificed 5-month-old Igfbp3+/+:KrasG12D/+ and Igfbp3-/-KrasG12D/+ mice that had been backcrossed to S129 background for representative analysis. The lung tissue was immediately removed after the mice were sacrificed and visible pleural nodules were counted directly."
item1003 .
item1004 "3 to 5-week old female BALB/c athymic (NU/NU) nude mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into indicated groups (five mice per group). To assess the in vivo effects of cycloleucine, the xenografted tumors had reached approximately 5 mm in diameter from mice and then these xenografted mice were feed with Vehicle or cycloleucine (25 mg/kg twice weekly) and tumor volume were measured every 3 day."
item1005 .
item1006 .
item1007 .
item1008 "BALB/c nude mice (5 weeks old) were purchased from the Beijing HFK Bioscience Co. Ltd. 2×106 cells (50 uL) were mixed with 50 uL Matrigel (BD Biosciences, San Jose, CA, USA) were injected subcutaneously in the rear flank fat pad of the nude mice (N = 6, per group)."
item1009 .
item1010 .
item1011 "HepG2 wild-type, KO1# 1 and KO1# 2 cells (about 5 × 106) were used to establish subcutaneous (SC) xenograft tumor models. Tumor size was monitored weekly. Mice were sacrificed after 4 weeks, and tumor weight and two dimensions of tumors were measured."
item1012 "2 × 105 dissociated cells in 2 uL PBS were injected into the following site (anteroposterior [AP] +0.6 mm, mediolateral [ML] +1.6 mm, and dorsoventricular [DV] 2.6 mm) with a rate of 1 uL/min."
item1013 "2 × 105 dissociated cells in 2 uL PBS were injected into the following site (anteroposterior [AP] +0.6 mm, mediolateral [ML] +1.6 mm, and dorsoventricular [DV] 2.6 mm) with a rate of 1 uL/min."
item1014 "For high-fat-diet treatment, 3-week-old male mice were randomly divided into three groups: two experimental groups freely fed with HFD of 60% fat, 20% carbohydrate, and 20% protein (Cat#: D12492, Research Diets) or KD of 89.5% fat, 0.1% carbohydrate, and 10.4% protein (Cat#: D12369B, Research Diets), and the control group fed with SD of 10% fat, 70% carbohydrate, and 20% protein (Cat#: D12450B, Research Diets), respectively. The mice were allowed free access to water and food.For fasting treatment, 3-month-old male mice were randomly divided into two groups: the fasting group with only free access to water for 48 h and the control group allowed free access to water and SD."
item1015 .
item1016 .
item1017 .
item1018 .
item1019 "The NSGS mice were bred and subjected to the xeno-transplantation model. For the AML mouse model, 0.2 × 106 MONOMAC6 cells were directly transplanted into NSGS mice via tail vein. After 10 days, FB23-2 (2 mg/kg/day) and DMSO vehicle were intraperitoneally injected into the mice for a continuous 10 days. The mice were euthanized by CO2 inhalation if they exhibited classical AML symptoms including hunched posture, paralysis, and reduced body weight. Meanwhile, the PB, spleen, and liver samples were collected for further analysis."
item1020 "The NSGS mice were bred and subjected to the xeno-transplantation model. For the AML mouse model, 0.2 × 106 MONOMAC6 cells were directly transplanted into NSGS mice via tail vein. After 10 days, FB23-2 (2 mg/kg/day) and DMSO vehicle were intraperitoneally injected into the mice for a continuous 10 days. The mice were euthanized by CO2 inhalation if they exhibited classical AML symptoms including hunched posture, paralysis, and reduced body weight. Meanwhile, the PB, spleen, and liver samples were collected for further analysis."
item1021 .
item1022 "The mice were fed a normal chow diet (Harlan Teklad) and maintained in a standard 12-hour light/12-hour dark cycle. At the age of 10 weeks, both male and female mice were intraperitoneally injected with 250 L (20 mg/mL) tamoxifen every other day for three times to delete Mettl14 in Beta-cells. Intraperitoneal glucose tolerance tests were performed on mice at the age of 15 weeks after a 5-hour fast (2 g/kg dextrose). Insulin levels were measured at 0, 15, and 30 minutes after glucose challenge by using the Ultra Sensitive Mouse Insulin ELISA Kit .Insulin tolerance tests were performed after a 5-hour fast by administering human recombinant insulin (0.75 U/kg)."
item1023 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
item1024 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
item1025 "Exposed Sprague-Dawley rats to 0, 250, and 500 mg DEHP per kg body weight per day at the prepuberty stage from postnatal day 22 (PND 22) to PND 35 by oral gavage."
item1026 "Exposed Sprague-Dawley rats to 0, 250, and 500 mg DEHP per kg body weight per day at the prepuberty stage from postnatal day 22 (PND 22) to PND 35 by oral gavage."
item1027 .
item1028 "Thirty-two C57BL/6J male mice were randomly assigned to a CON (basal diet), RES (basal diet + 500 mg/kg resveratrol), AFB1 (basal diet + 600 ug/kg aflatoxin B1), and ARE (basal diet + 500 mg/kg resveratrol and 600 ug/kg aflatoxin B1) group for 4 weeks of feeding (n = 8/group). Briefly, redox status, apoptosis, and m6A modification in the liver were assessed. Compared to the CON group, the AFB1 group showed increased activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), prevalent vacuolization and cell edema, abnormal redox status, imbalance apoptosis, and especially, the higher expression of cleaved-caspase-3 protein."
item1029 "Thirty-two C57BL/6J male mice were randomly assigned to a CON (basal diet), RES (basal diet + 500 mg/kg resveratrol), AFB1 (basal diet + 600 ug/kg aflatoxin B1), and ARE (basal diet + 500 mg/kg resveratrol and 600 ug/kg aflatoxin B1) group for 4 weeks of feeding (n = 8/group). Briefly, redox status, apoptosis, and m6A modification in the liver were assessed. Compared to the CON group, the AFB1 group showed increased activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT), prevalent vacuolization and cell edema, abnormal redox status, imbalance apoptosis, and especially, the higher expression of cleaved-caspase-3 protein."
item1030 .
item1031 "Zebrafish (Danio rerio) AB strain-derived Tg(lfabp:Dendra2-NTR)cq1 was used as WT, and rbm15cq96 mutant was generated by ENU treatment."
item1032 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1033 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1034 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1035 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1036 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1037 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1038 "For each experiment, 6- to 8-week-old mice were used and randomly allocated to each group. For xenograft mouse, 0.1 × 106 MA9.3ITD cells were transplanted into NRGS recipient mice intravenously. Drug treatment was started from 10 days after transplantation. CS2 was administered through intraperitoneal (i.p.) injection at 5mg/kg/day, every other day. CS1 dissolved in saturated Beta-cyclodextrin (C0926, Sigma-Aldrich) solution was delivered by intravenous injection (i.v.). Successful engraftment was observed following 4 weeks post inoculation displaying a level of about 5% human CD33+ cells in peripheral. To generate PDX mouse models, 1 × 106 AML patient derived BMMNCs were transplanted into NRGS recipient mice intravenously, and drug treatment was started from 7 days later. CS2, FB23-2, and free CS1 were administered through i.p. injection at 5 mg/kg/day, while Micelle (900661, Sigma-Aldrich) packaged CS1 was delivered by i.v. injection at 5mg/kg/day. Both CS1 and CS2 were injected every other day for a total of ten times."
item1039 .
item1040 .
item1041 .
item1042 "For in vivo drug treatment studies, intracranial xenografts were generated by implanting 5000 patient-derived GSCs (387 and 4121) into the right cerebral cortex of NSG mice as described above."
item1043 "For in vivo drug treatment studies, intracranial xenografts were generated by implanting 5000 patient-derived GSCs (387 and 4121) into the right cerebral cortex of NSG mice as described above."
item1044 "To establish a subcutaneous tumour model in nude mice, 2 × 107 Y79 cells (METTL3 knockdown group: shNC, shRNA1 and shRNA2; METTL3 up-regulated group: NC and METLL3) were resuspended in 1 mL of pre-cooled PBS, and 200 uL of the cell suspension was injected subcutaneously into the left side of the armpit to investigate tumour growth (4 × 106 per mouse)."
item1045 "To establish a subcutaneous tumour model in nude mice, 2 × 107 Y79 cells (METTL3 knockdown group: shNC, shRNA1 and shRNA2; METTL3 up-regulated group: NC and METLL3) were resuspended in 1 mL of pre-cooled PBS, and 200 uL of the cell suspension was injected subcutaneously into the left side of the armpit to investigate tumour growth (4 × 106 per mouse)."
item1046 "To establish a subcutaneous tumour model in nude mice, 2 × 107 Y79 cells (METTL3 knockdown group: shNC, shRNA1 and shRNA2; METTL3 up-regulated group: NC and METLL3) were resuspended in 1 mL of pre-cooled PBS, and 200 uL of the cell suspension was injected subcutaneously into the left side of the armpit to investigate tumour growth (4 × 106 per mouse)."
item1047 "To establish a subcutaneous tumour model in nude mice, 2 × 107 Y79 cells (METTL3 knockdown group: shNC, shRNA1 and shRNA2; METTL3 up-regulated group: NC and METLL3) were resuspended in 1 mL of pre-cooled PBS, and 200 uL of the cell suspension was injected subcutaneously into the left side of the armpit to investigate tumour growth (4 × 106 per mouse)."
item1048 "To establish a subcutaneous tumour model in nude mice, 2 × 107 Y79 cells (METTL3 knockdown group: shNC, shRNA1 and shRNA2; METTL3 up-regulated group: NC and METLL3) were resuspended in 1 mL of pre-cooled PBS, and 200 uL of the cell suspension was injected subcutaneously into the left side of the armpit to investigate tumour growth (4 × 106 per mouse)."
item1049 "Cell suspension of SW48 or SW48/TP53 (5-7 passages, 1 × 106 cells in 20 uL/mouse) was subcutaneously injected into the left flank of the mice."
item1050 .
item1051 .
item1052 .
item1053 .
item1054 .
item1055 .
item1056 .
item1057 .
item1058 .
item1059 .
item1060 .
item1061 .
item1062 "Left anterior descending coronary artery (LAD) was ligated for 20 minutes, followed by 48 h reperfusion. Controls underwent same procedures except LAD ligation. WTAP shRNA vector or its negative control (shNC) was injected into the left ventricular anterior wall 24 h before I/R. A pressure volume catheter was used for cardiac function assay."
item1063 "6- to 10-week-old female Rosa26Cas9/+ mice were treated daily for two weeks with either vehicle or 50 mg kg-1 STM2457 (STORM). Four weeks after treatment, bone marrow cells from these mice were freshly dissected (as mentioned above) and blocked with anti-mouse CD16/32 (BD Pharmigen, cat. no. 553142) and 10% mouse serum (Sigma)."
item1064 .
item1065 .
item1066 .
item1067 6- to 12-week-old mice were used for experiments.
item1068 .
item1069 .
item1070 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1071 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1072 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1073 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1074 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1075 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1076 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1077 "For MIA + SAH control, S-adenosylhomocysteine (SAH), Mettl3 inhibitor (10 mg/kg) (MCE, NJ, USA) was injected intraperitoneally before MIA injection and maintained twice a week until mice were sacrificed."
item1078 "5 × 105 HCT116 CSCs were subcutaneously injected into the mice similarly as nude mice. Seven days later, 5, 10, or 20 mg/kg Berberine was intraperitoneally injected. The same volume of PBS was injected and considered as negative control."
item1079 "Wild-type C57 (female, 12-16 weeks old), ALKBH5 /- mice (female and male, 12-16 weeks old), and SPF-grade SD rats (female, 180-230 g) were used to establish the AMI model.Sodium pentobarbital diluted to 10 mg/mL was used to anesthetize the mice or rat at the dose of 50 mg/kg through an intraperitoneal injection. By using a small animal ventilator with endotracheal intubation, thoracotomy was performed at the left fourth intercostal region. The heart was exposed, and the left anterior descending coronary artery (LCA) was occluded through a 6-0 silk suture that was placed 2-3 mm distal to the origin of the LCA with a slipknot. The apical region turned white, and ST segment elevation and T wave inversion of ECG showed that the AMI model was successfully established. Forty-five minutes after ischemia, the slipknot was released, and the ischemic region was reperfused. PBS (0.2 ml), HSSS (23.5 mg/kg, 0.2 ml), IOX1 (10 mg/kg, 0.2 ml), and HSSS-I (33.5 mg/kg, containing 10 mg/kg IOX1, 0.2 ml) were administered through caudal vein injection for 14 days at the frequency of one time per day."
item1080 "Wild-type C57 (female, 12-16 weeks old), ALKBH5 /- mice (female and male, 12-16 weeks old), and SPF-grade SD rats (female, 180-230 g) were used to establish the AMI model.Sodium pentobarbital diluted to 10 mg/mL was used to anesthetize the mice or rat at the dose of 50 mg/kg through an intraperitoneal injection. By using a small animal ventilator with endotracheal intubation, thoracotomy was performed at the left fourth intercostal region. The heart was exposed, and the left anterior descending coronary artery (LCA) was occluded through a 6-0 silk suture that was placed 2-3 mm distal to the origin of the LCA with a slipknot. The apical region turned white, and ST segment elevation and T wave inversion of ECG showed that the AMI model was successfully established. Forty-five minutes after ischemia, the slipknot was released, and the ischemic region was reperfused. PBS (0.2 ml), HSSS (23.5 mg/kg, 0.2 ml), IOX1 (10 mg/kg, 0.2 ml), and HSSS-I (33.5 mg/kg, containing 10 mg/kg IOX1, 0.2 ml) were administered through caudal vein injection for 14 days at the frequency of one time per day."
item1081 "The immunoprecipitated RNA samples from sham and 12h reperfusion groups (n = 5 each) were labeled with Cy5 fluorescent dye using Super RNA Labeling Kit (Arraystar) and purified using RNeasy Mini Kit. Cy5 labeled cRNAs were fragmented and hybridized to a mouse m6A epitranscriptomic microarray (8×60K, Arraystar) that contained 44,122 mRNA and 12,496 lncRNA degenerate probes. The hybridized arrays were scanned using an Agilent Scanner G2505C. For each of the 2 groups, 5 microarrays were used."
item1082 SMC-specific TWIST1-deficient mice were generated by crossing TWIST1flox/flox mice with animals expressing Sm22-Cre (smooth muscle protein 22-Cre). Deletion of TWIST1 in SMCs was confirmed by histology and Western blot.
item1083 .
item1084 "The thoracic cavity of rats was exposed, and the left anterior descending coronary artery was ligated with a 6-0 silk thread. Successfully surgical MI could be observed, with myocardium color fading and pulse weakening. After 30 min of ischemia, the blood flow was restored by releasing the slipknot, and then 120-min perfusion was performed. Afterward, the thoracic cavity of rats was sutured. The rats were assigned into 4 groups, with 12 rats in each group. Lentivirus packaged short hairpin (sh)-negative control (NC) and sh-METTL3 (GenePharma, Shanghai, China) were injected into the rats via tail vein 24 h before operation. The titer of lentivirus was 1 × 109 TU/mL, and the injection rate was 0.2 ul/min for 10 min. Blood samples were collected 24 h after reperfusion."
item1085 "The thoracic cavity of rats was exposed, and the left anterior descending coronary artery was ligated with a 6-0 silk thread. Successfully surgical MI could be observed, with myocardium color fading and pulse weakening. After 30 min of ischemia, the blood flow was restored by releasing the slipknot, and then 120-min perfusion was performed. Afterward, the thoracic cavity of rats was sutured. The rats were assigned into 4 groups, with 12 rats in each group. Lentivirus packaged short hairpin (sh)-negative control (NC) and sh-METTL3 (GenePharma, Shanghai, China) were injected into the rats via tail vein 24 h before operation. The titer of lentivirus was 1 × 109 TU/mL, and the injection rate was 0.2 ul/min for 10 min. Blood samples were collected 24 h after reperfusion."
item1086 .
item1087 About 5 × 106 MKN45 cells stably transfected with IGF2BP2 shRNA or sh-NC vectors were subcutaneously injected into flank of nude mice.
item1088 .
item1089 .
item1090 .
item1091 "Each mouse was anaesthetized with inhaled isoflurane, and the left proximal ureter was exposed. Then, the ureter was ligated with 6-0 silk thread and severed. In the sham operation group, the left ureters of mice were exposed, but not ligated or severed. The 3rd, 7th and 14th days after surgery were the time points for killing. At each time point, a total of 10 mice in the UUO group were executed, and a total of 10 mice in the sham group were also executed to serve as controls."
item1092 "NOD/SCID mice (6-week-old) were injected (subcutaneously in both flanks) with 5.0 x 106 PANC-1 GemR cells (infected with scr or METTL14 shRNA) per mouse suspended in 50 ul PBS and mixed with equal volume of growth factor reduced matrigel. One week after injection, we started measuring tumor size at the indicated times. Tumor size was calculated by 0.5 × (long diameter) × (short diameter)2. The mice were treated with vehicle or 100 mg/kg gemcitabine intraperitoneally twice a week."
item1093 "The clone, with one wild-type Mettl3 allele and one L1L2_Bact_P cassette inserted allele, was injected into C57BL/6 blastocysts. Mettl3-targeted mouse line was established from a germline-transmitting chimera. The chimeric mouse was crossed to C57BL/6 Flp mice to excise the neomycin resistance system."
item1094 "The normal group consisted of C57BL/6J mice on normal diet and the HFD group consisted of ApoE C57BL/6J mice on HFD (containing 10% lard oil, 4% milk powder, 2% cholesterol, and 0.5% sodium cholate). Four weeks after HFD feeding, the mice were injected with 200 ul lentivirus containing 1 × 10-/--/-8 TU (lentivirus carrying sh-METTL3 or sh-NC; designed and constructed by GENCHEM (Shanghai, China)) via tail vein. Six weeks after transfection, all mice were euthanized by a tail vein injection of 200 mg/kg pentobarbital sodium."
item1095 .
item1096 "METTL3 knockout or control CT26 and MC38 cells were injected subcutaneously into the dorsal flank of each 4- to 6-week-old male immunocompetent BALB/c and C57BL/6 mice, respectively. Anti-Gr-1 (BE0075; Bio-X-Cell, Lebanon, NH) or immunoglobulin (Ig)G isotype control (BE0090, Bio-XCell) was given every other day via intraperitoneal injection (150 ug/mouse). SB-265610 (Tocris, Bristol, UK) or phosphate-buffered saline was administrated through intraperitoneal injections at a dosage of 2 mg/kg per day. Tumor sizes were measured every other day. To establish an orthotopic mouse model of CRC, 5- to 6-week-old male C57BL/6 mice were treated with 1.7% dextran sodium sulfate in drinking water for 5 days, and then allowed to recover for 3 days. After 24 hours of fasting, METTL3 knockout or control MC38 cells suspended in 50 ul of 1 mg/mL Matrigel-phosphate-buffered saline (Corning, Corning, NY) were instilled into the colon lumen of anesthetized mice, coated sparingly with Vaseline."
item1097 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
item1098 YTHDF3-/- mice were generated using the CRISPR-Cas9 system.
item1099 "For the subcutaneous implantation model, 1 × 106 Cal27 cells stably transduced with lentivirus were injected into the left or right flanks of BALB/c nude mice (aged 4-6 weeks). Following stable transfection, 2 × 105 SCC7 cells were subcutaneously inoculated into C3H mice (aged 6-8 weeks)."
item1100 .
item1101 "Based on our study design, the enrolled animals were blindingly grouped and received the following treatments: (1)Sham-NC vectors, (2)Sham-sh-METTL3, (3)Sham-sh-METTL3 + miR-150, (4)Sham-sh-METTL3 + Lv-YTHDF2, (5)Sham-sh-METTL3 + sh-BDNF, (6)Sham-anti-miR150, (7)Sham-anti-miR150+sh-BDNF, (8)SNI-Lv-METTL3, (9)SNI-Lv-METTL3 + anti-miR-150, (10)SNI-Lv-METTL3 + sh-YTHDF2, (11)SNI-Lv-METTL3 + Lv-BDNF, (12)SNI-miR150, (13)SNI-miR150+Lv-BDNF. The pain behaviors were detected in the respective time points and the expressions of METTL3 and miR-150 were determined at day 14 after the rats were sacrificed."
item1102 "After general anesthesia with an intraperitoneal injection of ketamine (80 mg/kg) and xylazine (4 mg/kg), topical application of 0.5% proparacaine ophthalmic solution was conducted. Three sutures (10-0 nylon) were placed intrastromally between the limbus and corneal center at the 4, 8, and 12 o'clock positions. Topical norfloxacin was applied after surgery."
item1103 .
item1104 "The clone, with one wild-type Mettl3 allele and one L1L2_Bact_P cassette inserted allele, was injected into C57BL/6 blastocysts. Mettl3-targeted mouse line was established from a germline-transmitting chimera. The chimeric mouse was crossed to C57BL/6 Flp mice to excise the neomycin resistance system."
item1105 .
item1106 .
item1107 .
item1108 .
item1109 .
item1110 .
item1111 .
item1112 .
item1113 .
item1114 .
item1115 "Mettl3flox/flox mice were crossed with the transgenic Cdh5-CreERT2 mice to generate the Mettl3-ecKO mice. Cdh5-Cre Mettl3flox/flox mice received an intragastric injection of 50 ul tamoxifen (1 mg/mL) at P1-P3 and P5 for Cre activation and Mettl3 knockout. After Mettl3 knockout, the mouse pups and their nursing mothers were exposed to 75% oxygen (hyperoxia) from P7 to P12 in an incubator chamber. Then, the pups were returned to normal oxygen conditions (normoxia)."
item1116 .
item1117 .
item1118 "Mice bearing the Alkbh5-floxed allele (Alkbh5 fl/fl , Cyagen) were crossed with transgenic mice expressing Cre recombinase under the control of the Myl1 promoter (Myl1-Cre; Stock No: 024713, The Jackson Laboratory) to generate muscle-specific Alkbh5 knockout mice (Myl1-Cre;Alkbh5 fl/fl ). Littermate Alkbh5fl/fl mice were used as controls. Genotyping by tail DNA and PCR were performed at 4 weeks of age."
item1119 Twenty 5-week-old male nude mice were randomly divided into two groups and injected with either 2B-NNK or 2B-C cells. Tumor growth was measured every 3 days.
item1120 "Mice were randomized into several groups. For the subcutaneous implantation model, 1 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks). For lung colonization assays, 1 × 106 cells were injected into the tail vein of female NOD/SCID mice (6-7 weeks), and 6 weeks later the lung was removed and fixed with 10% formalin."
item1121 .
item1122 "HCC cells stably transfected with empty vector or circCPSF6 were subject to construct animal models, followed by the regular treatment of verteporfin, an inhibitor of YAP signaling."
item1123 "For the in vivo cell proliferation assay, A549 and SPCA1 cells were stably transfected with sh-Ctrl and sh-KIAA1429 using lentivirus (GeneChem, Shanghai, China). The cells were subcutaneously injected into either side of the posterior flanks of the mouse. The tumor volume was measured every few days (length×width2×0.5)."
item1124 .
item1125 "5 × 106 Huh7 and HCC-LM3 cells resuspended in 100ul PBS were subcutaneously injected to the left flank of the mice (randomly selected, five mice per group for Huh7 cells in the first time and ten mice per group for HCC-LM3 cells in the second time. No blinding was performed)."
item1126 "Cas9 and sgRNA were microinjected into the fertilized eggs of C57BL/6J mice, which were then transplanted to obtain positive F0 mice. The statuses of F0 mice were confirmed by PCR and sequencing. Next, positive F0 mice were mated with C57BL/6J mice to yield stable F1 generation mice. F1 and F2 transgenic mice were used in this study."
item1127 "The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment."
item1128 "The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment."
item1129 "For the subcutaneous implantation ICC mouse model, 6-week-old male NCG mice (Jiangsu, China) were randomly enrolled into shNC group and shYTHDF1 group (n = 9); 1 × 106 HuCCT1 cells in 0.1-mL PBS transfected with shNC or shYTHDF1 were subcutaneously inoculated in the right flanks of the mice. For AKT/YapS127A-induced orthotopic ICC mouse model, 16 mice were divided into two groups randomly. For the control group, 20-ug AKT, 30-ug Yap, and 2-ug pCMV/SB plasmids plus 20-ug vector plasmids as control were diluted in 2-mL saline and then were injected into the lateral tail vein within 7 s. For the YTHDF1-overexpressed group, mice were injected with additional 20-ug YTHDF1 plasmids under the same conditions. Mice were sacrificed at 4 weeks after injection, and liver tissues were harvested for analysis."
item1130 .
item1131 .
item1132 .
item1133 .
item1134 FTO over-expression db/db mice were generated through injecting the tail vein with Fto-overexpression lentivirus at 12 weeks.
item1135 .
item1136 The PC cell line PANC1 was subcutaneously injected into the dorsal flank of the mice at the concentration of 1 × 106 cells per mouse.
item1137 .
item1138 The PC cell line PANC1 was subcutaneously injected into the dorsal flank of the mice at the concentration of 1 × 106 cells per mouse.
item1139 .
item1140 "First, subcutaneous transplanted model was used to evaluate the growth of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Second, subcutaneous transplanted model was used to evaluate the metastasis potential of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Third, the in vivo lung metastasis model was established by injecting with BT-549, BT-549LMF3, FTO stable BT-549LMF3, sh-METTL3 BT-549LMF3, and sh-KRT7 BT-549LMF3 stable cells (1 × 106 per mouse, n = 5 for each group)."
item1141 "First, subcutaneous transplanted model was used to evaluate the growth of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Second, subcutaneous transplanted model was used to evaluate the metastasis potential of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Third, the in vivo lung metastasis model was established by injecting with BT-549, BT-549LMF3, FTO stable BT-549LMF3, sh-METTL3 BT-549LMF3, and sh-KRT7 BT-549LMF3 stable cells (1 × 106 per mouse, n = 5 for each group)."
item1142 "Xenograft tumor formation was observed in 5 of 5 and 3 of 5 animals when 1 × 105 and 1 × 104 sorted LGR5+ cells, were subcutaneously injected into nude mice, respectively."
item1143 .
item1144 "Mice were injected subcutaneously with LoVo (1 × 106) cells, which were stably transfected with the control shRNA or YTHDF1 shRNA."
item1145 .
item1146 .
item1147 "For tumour xenograft models, 1 × 107 HuCC-T1 cells in knockdown group or control group were implanted into the right flank of 5-week-old female nude mice. The volumes of tumour were recorded every 4 days by calliper. The volumes were calculated as length × width2/2. For patient-derived xenograft (PDX) model (PDX0075), ICC tissues from a patient, who relapsed in 6 months after R0 resection and subsequent chemotherapy with cisplatin and gemcitabine, were diced into 3 mm3 pieces and transplanted subcutaneously into the right flank of 5-week-old female B-NDG mice."
item1148 .
item1149 "For tumor growth assay, 4 × 106 logarithmically growing GIST cells were transfected with T1S-vector, T1S-METTL3, 882S-vector, or 882S-METTL3 constructs, and subcutaneously injected in 100 ul of PBS into the flank of female nude mice(4-week-old). Mice were then randomly divided into 8 groups (n = 5 in each group): (1) injected with T1S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water); (2) injected with T1S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (3) injected with T1S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water); (4) injected with T1S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (5) injected with 882S-vector-harboring cells and treated with imatinib (600 mg/l in drinking water); (6) injected with 882S-vector-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water); (7) injected with 882S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water); and (8) injected with 882S-METTL3-harboring cells, and treated with imatinib (600 mg/l in drinking water) and MRP1 inhibitor (100 mg/l in drinking water)."
item1150 "For subcutaneous transplanted model, sh-control and sh-METTL3 HCT-116/5-FU cells (5 × 106 per mouse) were diluted in 100ul PBS + 100 ul Matrigel (BD Biosciences, San Jose, CA, USA) and injected subcutaneously in the rear flank fat pad of the nude mice."
item1151 "A total of 5 × 106 cells in 200 ul PBS were injected subcutaneously into the flanks of nude mice. After injection, cisplatin treatment was initiated on day 5. Mice were injected with 5 mg/kg cisplatin or PBS solution in the abdominal cavity once a week for 3 weeks."
item1152 .
item1153 Using the diabetic rat model established by HGHF feeding with a subsequent intraperitoneal injection of a single low dose of streptozocin.
item1154 .
item1155 .
item1156 .
item1157 .
item1158 Mettl3flox/flox and Mettl3-HKO mice were fasted overnight and then injected intraperitoneally with 20 uM BODIPY FL C16 in 200 ul saline for 20 min.
item1159 .
item1160 Mettl3flox/flox and Mettl3-HKO mice were fasted overnight and then injected intraperitoneally with 20 uM BODIPY FL C16 in 200 ul saline for 20 min.
item1161 .
item1162 "For the tumor xenograft, mice were randomly divided into three groups with four mice for each group. Then, 1 × 106 cells after indicated treatment were harvested and resuspended in 50 ul of PBS. Then the cells were subcutaneously injected into the right front flank of each mouse."
item1163 .
item1164 "For CDX model, nude mice (female, 4-6-week-old) were subcutaneously injected with 5 × 106 HCT116 cells on the both flank. For PDX model, the patient tumors were divided into small pieces and then inoculated on both flank of nude mice. For knockdown FTO mice model, FTO mice model, two weeks after inoculation, the shFTO#3 lenti-virus injected into the tumor for three consecutive days. For combined medication mice model, intraperitoneal injection of Rhein and Olaparib was started one week after inoculation."
item1165 "For CDX model, nude mice (female, 4-6-week-old) were subcutaneously injected with 5 × 106 HCT116 cells on the both flank. For PDX model, the patient tumors were divided into small pieces and then inoculated on both flank of nude mice. For knockdown FTO mice model, FTO mice model, two weeks after inoculation, the shFTO#3 lenti-virus injected into the tumor for three consecutive days. For combined medication mice model, intraperitoneal injection of Rhein and Olaparib was started one week after inoculation."
item1166 .
item1167 5 × 106 SW1990 cells expressing NUCB1 (oeNUCB1) or control vector (oeNC) were injected subcutaneously.
item1168 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1169 .
item1170 Male nu/nu mice between 4 and 6 weeks of age received subcutaneous injections of equivalent AGS cells expressing either control or LV-HOXB13 within 30 min of harvesting on the right and left flanks.
item1171 "Twenty-four specific pathogen free female BALB/c nude mice (age: 6 weeks, weight: 15 ~ 18 g) were purchased from Slac Laboratory Animal Co., Ltd., and subcutaneously injected with SW620 cells stably transfected with oe-NC, oe-GSK3-Beta + oe-NC, or oe-GSK3-Beta + oe-c-Myc to establish a subcutaneous xenograft tumour model in nude mice."
item1172 "Twenty-four specific pathogen free female BALB/c nude mice (age: 6 weeks, weight: 15 ~ 18 g) were purchased from Slac Laboratory Animal Co., Ltd., and subcutaneously injected with SW620 cells stably transfected with oe-NC, oe-GSK3-Beta + oe-NC, or oe-GSK3-Beta + oe-c-Myc to establish a subcutaneous xenograft tumour model in nude mice."
item1173 .
item1174 .
item1175 Approximately 5 × 106 253J and 5637 cells infected with indicated vectors were injected subcutaneously into the flank of the mice.
item1176 "Homologous recombination was used, cas9 mRNA, gRNA, and donor vector were microinjected into the fertilized eggs of C57BL/6J mice to obtain F0 generation mice."
item1177 .
item1178 Each mouse was injected subcutaneously with 5 × 106 units of tumor cells to construct the tumor model.
item1179 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1180 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old)."
item1181 .
item1182 .
item1183 "Circ_0008542, where rats were injected with exosomes containing circ_0008542 into the tail vein for 8 weeks."
item1184 "Circ_0008542, where rats were injected with exosomes containing circ_0008542 into the tail vein for 8 weeks."
item1185 .
item1186 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
item1187 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
item1188 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
item1189 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
item1190 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1191 .
item1192 .
item1193 "The mice were subcutaneously inoculated with 786-O cells stably transfected with lentiviruses carrying sh-NC/sh-circMET, respectively (5 × 106, 200 uL)."
item1194 .
item1195 .
item1196 "For tumor xenograft studies, 143B cells stably expressing scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected subcutaneously into the flanks of 4-week-old athymic nude mice."
item1197 "For tumor xenograft studies, 143B cells stably expressing scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected subcutaneously into the flanks of 4-week-old athymic nude mice."
item1198 "Twenty mice were randomly divided into three groups: normal mice group (N), diabetic mice group (DM), and diabetic mice administrated with TSA group (DM + TSA)."
item1199 .
item1200 "For the studies of investigating mice survival, mice were intracranially injected with 10,000 GSC11, 10,000 GSC7-2, or 500,000 LN229 cells."
item1201 5 × 106 infected T98G cells (LV-NC or LV-YTHDF2) were injected into the flanks of mice through subcutaneous.
item1202 "For the studies of investigating mice survival, mice were intracranially injected with 10,000 GSC11, 10,000 GSC7-2, or 500,000 LN229 cells."
item1203 "Fresh PDX tumor samples collected from two established PDX models (PDX #07 with high TRIB2 expression and PDX #12 with low TRIB2, passages three to four) were minced and subcutaneously implanted into the flanks of 3- to 4-week-old female BALB/c nude mice (Jiesijie Laboratory Animals)."
item1204 "Fresh PDX tumor samples collected from two established PDX models (PDX #07 with high TRIB2 expression and PDX #12 with low TRIB2, passages three to four) were minced and subcutaneously implanted into the flanks of 3- to 4-week-old female BALB/c nude mice (Jiesijie Laboratory Animals)."
item1205 .
item1206 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
item1207 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
item1208 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
item1209 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
item1210 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
item1211 .
item1212 5 × 106 infected T98G cells (LV-NC or LV-YTHDF2) were injected into the flanks of mice through subcutaneous.
item1213 .
item1214 .
item1215 A total of 5 × 106 786O cells were subcutaneously injected into the left flanks of the mice.
item1216 Mice with a Tmem30a deletion specifically in pancreatic beta cells were generated as previously described. Mice developed with NAFLD were named for Tmem30a-associated NAFLD (TAN) mice. The littermate mice with genotypes of Tmem30aloxP/loxP were used as controls.
item1217 Mice with a Tmem30a deletion specifically in pancreatic beta cells were generated as previously described. Mice developed with NAFLD were named for Tmem30a-associated NAFLD (TAN) mice. The littermate mice with genotypes of Tmem30aloxP/loxP were used as controls.
item1218 Mice with a Tmem30a deletion specifically in pancreatic beta cells were generated as previously described. Mice developed with NAFLD were named for Tmem30a-associated NAFLD (TAN) mice. The littermate mice with genotypes of Tmem30aloxP/loxP were used as controls.
item1219 Mice with a Tmem30a deletion specifically in pancreatic beta cells were generated as previously described. Mice developed with NAFLD were named for Tmem30a-associated NAFLD (TAN) mice. The littermate mice with genotypes of Tmem30aloxP/loxP were used as controls.
item1220 "5 × 105 cells were injected subcutaneously into the right axilla of mice. Tumor volume was measured by a caliper weekly and calculated as length × width2 × 0.52. For the liver orthotopic-implanted models, each liver of mice was injected with 1 × 106 cells."
item1221 1 × 105 HNE2 cells (with or without METTL3 knockdown) were labeled with luciferase gene and injected into the tail vein of the nude mice.
item1222 1 × 105 HNE2 cells (with or without METTL3 knockdown) were labeled with luciferase gene and injected into the tail vein of the nude mice.
item1223 "For the xenograft tumor model, approximately 1 × 106 ccRCC cells suspended in 100 uL PBS were subcutaneously inoculated in the right flank of 5-week-old BALB/c nude mice. For the ccRCC orthotopic implantation model, approximately 1 × 106 ccRCC cells suspended in 30 uL Matrigel were injected under the renal capsule of 5-week-old BALB/c nude mice. After 6 weeks, the anesthetized mice were intraperitoneally injected with D-luciferin (Yeason) and imaged using an in vivo imaging system to detect tumor growth and metastasis. For the lung metastasis model, approximately 5 × 105 ccRCC cells suspended in PBS were injected into the tail vein of 5-week-old mice. After 6-8 weeks, mice were anesthetized and lung metastasis was imaged as above."
item1224 1 × 105 HNE2 cells (with or without METTL3 knockdown) were labeled with luciferase gene and injected into the tail vein of the nude mice.
item1225 1 × 105 HNE2 cells (with or without METTL3 knockdown) were labeled with luciferase gene and injected into the tail vein of the nude mice.
item1226 "A total of 1 × 106 luciferase-labeled BC cells transfected with shWTAP or shNC were injected subcutaneously with or without C5aR1 neutrophils (tumor cells:neutrophils, 10:1)."
item1227 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1228 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1229 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1230 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1231 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1232 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1233 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1234 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1235 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1236 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1237 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1238 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1239 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
item1240 "Equal amount of HCT116 cells (2 × 106) stably expression of relevant plasmids was injected into the right flank of mice, tumor bulks was monitored once a week after injection and volumes were counted as 0.5 × a2 × b (a and b respectively indicated short and long diameter of tumor)."
item1241 "Equal amount of HCT116 cells (2 × 106) stably expression of relevant plasmids was injected into the right flank of mice, tumor bulks was monitored once a week after injection and volumes were counted as 0.5 × a2 × b (a and b respectively indicated short and long diameter of tumor)."
item1242 A total of 106 LLC cells transfected with the following constructs were injected subcutaneously into C57BL/6 mice.
item1243 A total of 106 LLC cells transfected with the following constructs were injected subcutaneously into C57BL/6 mice.
item1244 .
item1245 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1246 .
item1247 "To establish the adjuvant-induced arthritis (AIA) model, the rats were given complete Freund's adjuvant (CFA; Chondrex, Inc.) on the left paw of 0.1 ml per 100 g of body weight. Additionally, the rats were injected with normal saline to create the negative control (NC) group."
item1248 "To establish the adjuvant-induced arthritis (AIA) model, the rats were given complete Freund's adjuvant (CFA; Chondrex, Inc.) on the left paw of 0.1 ml per 100 g of body weight. Additionally, the rats were injected with normal saline to create the negative control (NC) group."
item1249 "To establish the adjuvant-induced arthritis (AIA) model, the rats were given complete Freund's adjuvant (CFA; Chondrex, Inc.) on the left paw of 0.1 ml per 100 g of body weight. Additionally, the rats were injected with normal saline to create the negative control (NC) group."
item1250 .
item1251 .
item1252 .
item1253 .
item1254 .
item1255 2 × 106 stably transfected HeLa cells were subcutaneously inoculated into the left flank of mice.
item1256 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1257 2 × 106 stably transfected HeLa cells were subcutaneously inoculated into the left flank of mice.
item1258 The cells (1 × 106) were re-suspended in normal saline and mixed with 25% Matrigel matrix (50 uL) at a 1:1 ratio and subcutaneously injected into the right groin of the mice.
item1259 Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
item1260 Stably transfected shMETTL14 and shNC DU145 cells (5×106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
item1261 The vitreous cavity of the right eye of each rat was injected either with ARPE-19 cells (1 × 105 per uL in PBS [pH 7.4]) overexpressing METTL3 or control vector cells or with an equal volume of PBS (pH 7.4).
item1262 .
item1263 .
item1264 .
item1265 T24 cells were subcutaneously injected into the mice (1 x 106 cells / injecting site).
item1266 "2 × 106 U87MG cells already expressing shscr or shADAR1 were subcutaneously injected in the flank of 6-week-old nude mice (nu/nu, Charles River, Wilmington, MA, USA)."
item1267 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1268 "Male mice (8-10 weeks old, SLAC Laboratory Animal Co., Ltd., Shanghai, China) were administrated with STZ through intraperitoneal injection (I.P) for continuous 5 days (d)."
item1269 .
item1270 .
item1271 .
item1272 .
item1273 "To generate a highly metastatic orthotopic xenograft model, 1 × 105 luciferase-expressing Renca cells (Luc-Renca) in 25 ul of 2:1 (v/v) PBS:Matrigel were injected into the right sub-renal capsule of the kidney of BALB/c mice (4 mice/group)."
item1274 FTO-overexpressing and control cells (2 × 106 suspended in 100 ul PBS) were subcutaneously injected into each mouse.
item1275 FTO-overexpressing and control cells (2 × 106 suspended in 100 ul PBS) were subcutaneously injected into each mouse.
item1276 Female BALB/c nude mice aged 5 weeks (Beijing Vital River Laboratory Animal Technology) were randomly grouped and injected subcutaneously with 0.1 mL of cell suspension containing 2 × 106 PDAC cells in the back flank.
item1277 The mice were randomly divided into two groups which were inoculated with stable YTHDF2-expressing LUSC cells and the vector LUSC cells. A total of 5 × 106 of the cells were suspended in 0.1 ml of PBS and then injected subcutaneously into the flanks of mice.
item1278 5 × 106 SW1990 cells expressing NUCB1 (oeNUCB1) or control vector (oeNC) were injected subcutaneously.
item1279 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1280 "All the mice were randomly divided into three groups: the normal saline group (NS), the lipopolysaccharide + negative control group (LPS + NC), and the LPS + miR-29a-3p agomir group (LPS + Agomir)."
item1281 "The SKG mice were randomly divided into three groups: a PBS group, an Av-NC group, and an Av-ELMO1 group. The SKG mice in the Av-ELMO1 group were treated with 5 × 1010 Av-ELMO1 via intravenous tail vein injection at the time of disease induction, and the SKG mice in the Av-NC group or PBS group were separately treated with equal amounts of control adenoviruses or PBS."
item1282 .
item1283 .
item1284 "For the formation of xenograft tumors, 5 × 106 AGS cells mixed in Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) were subcutaneously injected into BALB/c nude mice (5-week-old male)."
item1285 .
item1286 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
item1287 "For the in vivo metastasis assays, luciferase labeled OS-RC-2 cells stably expressing OE-P2RX6 or pWPI-vector were injected into the tail vein of 5 weeks old BALB/c nude mice (Sipper-BK laboratory animal Company, Shanghai, China)."
item1288 "To generate the Mettl3+/- mice, 2 specific single-guide RNAs (sgRNAs) targeting exon 2 of Mettl3 were used, which resulted in a frameshift mutation and generated a premature stop codon."
item1289 "MG63 cells transduced with lentivirus expressing shTRIM7 or shNC, and SAOS2 cells transduced with lentivirus expressing TRIM7, BRMS1, TRIM7 plus BRMS1 or control vector, were injected via the tail vein into the nude mice (1 × 106 cells/mouse) (n = 11 per group)."
item1290 "100,000 pLKO and PARP1-sh1 (PT1 and PT2) cells were mixed with matrix gel and inoculate into BALB/C nude mice, respectively. After 25 days, 6 organoid transplanted tumor mice were treated with oxaliplatin (Sellekchem, s1224) twice a week for 4 weeks at a dose of 5 mg/kg."
item1291 "MG63 cells transduced with lentivirus expressing shTRIM7 or shNC, and SAOS2 cells transduced with lentivirus expressing TRIM7, BRMS1, TRIM7 plus BRMS1 or control vector, were injected via the tail vein into the nude mice (1 × 106 cells/mouse) (n = 11 per group)."
item1292 "MG63 cells transduced with lentivirus expressing shTRIM7 or shNC, and SAOS2 cells transduced with lentivirus expressing TRIM7, BRMS1, TRIM7 plus BRMS1 or control vector, were injected via the tail vein into the nude mice (1 × 106 cells/mouse) (n = 11 per group)."
item1293 "For subcutaneous xenograft models, 0.1 mL of cell suspension containing 106 cells were injected subcutaneously into the right flank of mice (n = 6 for each group)."
item1294 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
item1295 "Twenty-four female BALB/c athymic nude mice, which were 4-6 weeks old and weighed 20.0-25.0 g, were obtained from the Animal Research Center of PUMCH. WTAP-OE, WTAP-NC, shWTAP and shNC-lentivirus infected MIA PaCa-2 cells (5 × 106) were suspended in 50 uL PBS and then injected subcapsularly into the pancreatic tissue by 1-mL syringes."
item1296 "As to the subcutaneous transplanted model, WT Vec, Mettl3 Mut/-+vec, WTPDK4, Mettl3 Mut/-+vecPDK4 cells (2 × 10 6 per mouse, n = 10 for each group) were diluted in 200 uL normal medium + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
item1297 1 × 107 SK-HEP-1-Luc-shControl or SK-HEP-1-Luc-shMETTL3 stable cells were suspended in 300 uL of PBS and injected orthotopically into the left liver lobe of nude mice.
item1298 "The caudal vein injection mouse model, intrasplenic injection mouse model, and orthotopic xenograft IRFA HCC mouse models, including patient-derived xenograft (PDX), and cell-line-derived xenograft implantation models, were established as reported."
item1299 5 × 106 cells were suspended in 100 uL PBS and then were inoculated subcutaneously.
item1300 .
item1301 "100,000 pLKO and PARP1-sh1 (PT1 and PT2) cells were mixed with matrix gel and inoculate into BALB/C nude mice, respectively. After 25 days, 6 organoid transplanted tumor mice were treated with oxaliplatin (Sellekchem, s1224) twice a week for 4 weeks at a dose of 5 mg/kg."
item1302 .
item1303 .
item1304 .
item1305 .
item1306 .
item1307 .
item1308 .
item1309 .
item1310 .
item1311 .
item1312 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1313 "Modelled two groups of female 6-week-old BALB/C mice: severe asthma group and blank control group (n = 3 per group). They had the same feeding conditions and growth environment. Immunization solution: Dissolve 20 mg ovalbumin (OVA) in 1 ml normal saline (NS), after OVA is completely dissolved, dilute 0.4-10 ml and mix well, then it was mixed with the same volume of liquid aluminium adjuvant and placed on a shaking table at 4℃ for 30 min. Challenge solution: Add 0.5 g OVA into 10 ml NS, fully dissolve it, and shake it on a shaking table at 4℃ for 30 min. Immunization: Mice were injected intraperitoneally on days 0 and 12, each with 0.2 ml; the control group was treated with equal volume of normal saline. Challenge: On days 18-23, the mice were atomized by ultrasound in a closed container at a dose of 10 ml once a day for 20 min. Lung tissue was taken 24 h after the last atomization and immediately stored in liquid nitrogen."
item1314 "Modelled two groups of female 6-week-old BALB/C mice: severe asthma group and blank control group (n = 3 per group). They had the same feeding conditions and growth environment. Immunization solution: Dissolve 20 mg ovalbumin (OVA) in 1 ml normal saline (NS), after OVA is completely dissolved, dilute 0.4-10 ml and mix well, then it was mixed with the same volume of liquid aluminium adjuvant and placed on a shaking table at 4℃ for 30 min. Challenge solution: Add 0.5 g OVA into 10 ml NS, fully dissolve it, and shake it on a shaking table at 4℃ for 30 min. Immunization: Mice were injected intraperitoneally on days 0 and 12, each with 0.2 ml; the control group was treated with equal volume of normal saline. Challenge: On days 18-23, the mice were atomized by ultrasound in a closed container at a dose of 10 ml once a day for 20 min. Lung tissue was taken 24 h after the last atomization and immediately stored in liquid nitrogen."
item1315 .
item1316 .
item1317 .
item1318 .
item1319 .
item1320 .
item1321 "Animals were bred and housed in the pathogen-free facility of the Laboratory Animal Center of Shanghai General Hospital (Shanghai, China). All lungs were collected 4 weeks after BLM treatment for histology and further study. Lung microsections (5 uM) were applied to Masson's trichrome and Sirius red staining to visualize fibrotic lesions."
item1322 "Animals were bred and housed in the pathogen-free facility of the Laboratory Animal Center of Shanghai General Hospital (Shanghai, China). All lungs were collected 4 weeks after BLM treatment for histology and further study. Lung microsections (5 uM) were applied to Masson's trichrome and Sirius red staining to visualize fibrotic lesions."
item1323 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1324 "At 8 weeks of age, METTL14 cKO and WT mice were challenged with LPS (Sigma-Aldrich, St. Louis, MO; L2880, single intraperitoneal injection at 5 mg/kg, n = 3) or CCl4 (10%, Macklin, Shanghai, China; C805332, intraperitoneal injection at 5 mL/kg diluted with corn oil, twice per week for 4 weeks, n = 3). The corresponding control groups were treated with single intraperitoneal injection of saline (n = 3) or intraperitoneal injection of corn oil twice per week for 4 weeks (n = 3), respectively. Two hours after LPS injection and 4 weeks after CCl4 treatment, METTL14 cKO and WT mice were etherized and the primary KCs were isolated from liver according to a previously published method."
item1325 "At 8 weeks of age, METTL14 cKO and WT mice were challenged with LPS (Sigma-Aldrich, St. Louis, MO; L2880, single intraperitoneal injection at 5 mg/kg, n = 3) or CCl4 (10%, Macklin, Shanghai, China; C805332, intraperitoneal injection at 5 mL/kg diluted with corn oil, twice per week for 4 weeks, n = 3). The corresponding control groups were treated with single intraperitoneal injection of saline (n = 3) or intraperitoneal injection of corn oil twice per week for 4 weeks (n = 3), respectively. Two hours after LPS injection and 4 weeks after CCl4 treatment, METTL14 cKO and WT mice were etherized and the primary KCs were isolated from liver according to a previously published method."
item1326 "Liver-specific Bmal1f/f-AlbCre-knockout mice were purchased from Jackson Laboratory. C57BI/6J or Bmal1f/f-AlbCre-knockout male mice were maintained under a 12 hr light/12 hr dark (LD) cycle (ZT0 = 6 AM) and fed ad libitum with normal rodent chow (2018 Global 18% Protein diet, Envigo) and water. At 10-14 weeks of age, 10 male mice per group were sacrificed via CO2 asphyxiation at Zeitgeber Time (ZT) 0,2,6,10,12,14,18,22. In order to induce high levels of ROS in the liver, WT male mice were fasted 12 h and followed by intraperitoneal injection with 300 mg/kg APAP dissolved in PBS and re-fed."
item1327 "Liver-specific Bmal1f/f-AlbCre-knockout mice were purchased from Jackson Laboratory. C57BI/6J or Bmal1f/f-AlbCre-knockout male mice were maintained under a 12 hr light/12 hr dark (LD) cycle (ZT0 = 6 AM) and fed ad libitum with normal rodent chow (2018 Global 18% Protein diet, Envigo) and water. At 10-14 weeks of age, 10 male mice per group were sacrificed via CO2 asphyxiation at Zeitgeber Time (ZT) 0,2,6,10,12,14,18,22. In order to induce high levels of ROS in the liver, WT male mice were fasted 12 h and followed by intraperitoneal injection with 300 mg/kg APAP dissolved in PBS and re-fed."
item1328 "Liver-specific Bmal1f/f-AlbCre-knockout mice were purchased from Jackson Laboratory. C57BI/6J or Bmal1f/f-AlbCre-knockout male mice were maintained under a 12 hr light/12 hr dark (LD) cycle (ZT0 = 6 AM) and fed ad libitum with normal rodent chow (2018 Global 18% Protein diet, Envigo) and water. At 10-14 weeks of age, 10 male mice per group were sacrificed via CO2 asphyxiation at Zeitgeber Time (ZT) 0,2,6,10,12,14,18,22. In order to induce high levels of ROS in the liver, WT male mice were fasted 12 h and followed by intraperitoneal injection with 300 mg/kg APAP dissolved in PBS and re-fed."
item1329 "Liver-specific Bmal1f/f-AlbCre-knockout mice were purchased from Jackson Laboratory. C57BI/6J or Bmal1f/f-AlbCre-knockout male mice were maintained under a 12 hr light/12 hr dark (LD) cycle (ZT0 = 6 AM) and fed ad libitum with normal rodent chow (2018 Global 18% Protein diet, Envigo) and water. At 10-14 weeks of age, 10 male mice per group were sacrificed via CO2 asphyxiation at Zeitgeber Time (ZT) 0,2,6,10,12,14,18,22. In order to induce high levels of ROS in the liver, WT male mice were fasted 12 h and followed by intraperitoneal injection with 300 mg/kg APAP dissolved in PBS and re-fed."
item1330 .
item1331 .
item1332 .
item1333 .
item1334 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
item1335 .
item1336 .
item1337 .
item1338 .
item1339 .
item1340 .
item1341 .
item1342 .
item1343 .
item1344 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
item1345 "Leptin receptor-deficient (db/db) mice and control mice (db/ +) were purchased from Shanghai Model Organisms Center, which genetic background is C57BL/6J. All mice were used for experiments at 8-12 weeks old and were housed in constant 24 degrees cages with a 12 h alternating light/dark cycle and free access to water and food. To construct a diabetic heart disease model (DCM), mice were continuously fed to 24 weeks of age, then euthanized, hearts collected in 1.5 ml RNase-free centrifuge tubes, immediately immersed in liquid nitrogen to prevent RNA degradation, and finally stored at 80℃. Five pairs of db/db and db/ + heart samples were selected for RNA sequencing, and the remaining samples were saved for validation."
item1346 .
item1347 .
item1348 .
item1349 .
item1350 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1351 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1352 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1353 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1354 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1355 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
item1356 "For mouse muscle injury and regeneration experiment, tibialis anterior (TA) muscles of 6-week-old male mice were injected with 25 uL of 10 uM cardiotoxin (CTX, Merck Millipore, 217503), 0.9% normal saline (Saline) were used as control. The regenerated muscles were collected at day 1, 3, 5, and 10 post-injection. TA muscles were isolated for Hematoxylin and eosin staining or frozen in liquid nitrogen for RNA and protein extraction."
item1357 .
item1358 .
item1359 .
item1360 .
item1361 .
item1362 .
item1363 .
item1364 "Nude mice (4-6 week-old) were administered sterile water and feed in a specific pathogen-free barrier. Using a 1-mL syringe, 1 × 107 HEPG2 cells were subcutaneously inoculated into the right axilla of nude mice to build the HCC xenograft model. When the tumor volume reached 50 mm3, the nude mice were randomly divided into 1 control (n = 4) and 3 treatment groups (n = 4 each). RG7112, apatinib, and RG7112 + apatinib were administered to the treatment groups and an equal volume of dimethyl sulfoxide to the control group by daily gavage for 14 d. The tumor length (L) and width (W) were measured on alternate days using vernier calipers. The following formula was used to calculate the tumor volume: volume (mm3) = 0.5 × L × W × W. At the end of the experiment, the nude mice were killed by CO2 overdose anesthesia. The tumors were dissected and weighed using a precision balance, and the tumor tissue was stored in liquid nitrogen for further analysis."
item1365 "Nude mice (4-6 week-old) were administered sterile water and feed in a specific pathogen-free barrier. Using a 1-mL syringe, 1 × 107 HEPG2 cells were subcutaneously inoculated into the right axilla of nude mice to build the HCC xenograft model. When the tumor volume reached 50 mm3, the nude mice were randomly divided into 1 control (n = 4) and 3 treatment groups (n = 4 each). RG7112, apatinib, and RG7112 + apatinib were administered to the treatment groups and an equal volume of dimethyl sulfoxide to the control group by daily gavage for 14 d. The tumor length (L) and width (W) were measured on alternate days using vernier calipers. The following formula was used to calculate the tumor volume: volume (mm3) = 0.5 × L × W × W. At the end of the experiment, the nude mice were killed by CO2 overdose anesthesia. The tumors were dissected and weighed using a precision balance, and the tumor tissue was stored in liquid nitrogen for further analysis."
item1366 "Gemcitabine-resistant Panc1 cells (Panc1-GR) were prepared as stable luciferase clones after transduction with CTRL or shSRSF3 or sh-ANRIL-L vector or SRSF3 or ANRIL-L. For the PDX models, pieces of fresh human pancreatic cancer tissues were transplanted subcutaneously into the axilla of 4-6 week-old NOD/SCID mice."
item1367 "To test for malignant transformation, 1×107 cells were inoculated subcutaneously in the dorsal thoracic midline of ten NOD/SCID mice (Weitong Lihua Experimental Animal Technology Co. Ltd). Tumor formation and growth were assessed every 3 days."
item1368 "To test for malignant transformation, 1×107 cells were inoculated subcutaneously in the dorsal thoracic midline of ten NOD/SCID mice (Weitong Lihua Experimental Animal Technology Co. Ltd). Tumor formation and growth were assessed every 3 days."
item1369 "The male Sprague-Dawley rats (8 weeks old, 200-220 g) were purchased from the Laboratory Animal Center of Sun Yat-sen University. Streptozotocin (Sigma, USA) was given by intraperitoneal injection at a dose of 60 mg/Kg to induce diabetics rats, while the control rats were given by empty citrate buffer. One week after induction, those rats with blood glucose levels > 16.7 mmol/L for three times were considered as successful inducted diabetes. All the rats did not receive insulin during the experiments.In the intraocular injection experiments, rats confirmed as the DM model (blood glucose levels > 16.7 mmol/L for three times) were anesthetized with an intraperitoneal injection of sodium pentobarbital (50 mg/Kg). A total of 10 ul DMEM with 1*109 TU lentiviruses (A20-overexpression, OE-A20 group) or the same volume of DMEM with control lentiviruses (OE-NC group) was injected into the vitreous cavity using a 33-gauge needle. This treatment was performed one time per month, and the rats were sacrificed for further experiments at the 3 months."
item1370 .
item1371 .
item1372 .
item1373 .
item1374 .
item1375 .
item1376 .
item1377 "Established a xenograft model in BALB/c nude mice by inoculating HCC827-GR cells transfected with the constructs for circASK1 silencing, ASK1-272a.a overexpression and ASK1-272a.a overexpression/circASK1 knockdown."
item1378 .
item1379 .
item1380 .
item1381 .
item1382 .
item1383 .
item1384 "Sixteen BALB/c nude mice (male, 6-week-old) were raised under pathogen-free conditions and randomly divided into two groups. A total of 2 × 106 A375 NTC or sh-METTL3#2 cells were subcutaneously inoculated into the right hind flank. Body weight and tumor size were measured every other day. The tumors were harvested at the end of the observation period, and tumor weight and gross images were recorded (11 days after inoculation). The tumors were embedded in RNA later and 10% formalin for further detection."
item1385 "A total of 18 mice were randomly divided into 2 groups, a sham group (n=9), and a MIRI group (n=9). Operators were blinded to the allocation. The MIRI mice were established by ligation of the left anterior descending coronary artery. After peritoneal anesthesia with 1% pentobarbital sodium, surgical incisions were made between the third and fourth sternal intercostals to expose the heart. The left anterior descending coronary artery was identified under the microscope, and the left atrial appendage was ligated at the 1 cm lower margin with a suture needle to induce ischemia for 30 minutes. Then, the slip knot was unwound for 24 h of reperfusion. The sham group was not ligated. No unexpected adverse events were observed and animals were not excluded. Myocardium and peripheral blood were collected after the mice were sacrificed at 24 h reperfusion. Each group was analyzed in at least 3 independent experiments, which made the group size of each experiment 3."
item1386 .
item1387 .
item1388 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1389 "Established a xenograft model in BALB/c nude mice by inoculating HCC827-GR cells transfected with the constructs for circASK1 silencing, ASK1-272a.a overexpression and ASK1-272a.a overexpression/circASK1 knockdown."
item1390 .
item1391 .
item1392 .
item1393 .
item1394 "Cells at 1 × 106 were subcutaneously injected into the mice similarly to nude mice. Twenty-eight days later, grafted tumors were collected and morphologically analyzed."
item1395 "Cells at 1 × 106 were subcutaneously injected into the mice similarly to nude mice. Twenty-eight days later, grafted tumors were collected and morphologically analyzed."
item1396 .
item1397 "KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice."
item1398 "KP and Mettl3-/- mice were bred to generate KPM-/- mice. Afterwards, the KP and KPM-/- mice were intranasally infected under anesthesia with adeno-associated virus type 5 (AAV5) expressing Cre to initiate lung tumorigenesis along with ALKBH5-expressing AAV5 or Empty AAV5 to generate KPE, KPA, KPEM-/- and KPAM-/- spontaneous LUAD mouse models. For generation of LLC-based intra-pulmonary tumor mouse models, 1 × 107 LLC cells were injected into C57BL/6 mice via the tail vein.For cell-derived xenograft (CDX) mouse models, 1.0 × 107 H1299 or 1.5 × 107 H1975 cells were subcutaneously injected into 4-6-week-old athymic nude mice. The tumors were monitored at indicated time points and isolated for further analysis after sacrifice."
item1399 .
item1400 .
item1401 .
item1402 .
item1403 .
item1404 "A total of 5 × 106 transfected MKN-45 cells, stably transfected with sh-IGF2BP1 vector or empty vector were subcutaneously injected into the flank of the mice. Tumor growth was measured every three days, and calculated using the following equation = a × b2/2 (a for longitudinal diameter; and b for latitudinal diameter). Three weeks after injection, mice were sacrificed."
item1405 .
item1406 .
item1407 .
item1408 "The mice were randomly divided into control, Ad-sh-NC, and Ad-sh-METTL14 groups (10 mice per group). The mice in the control group were fed a normal diet, while the Ad-sh-NC and Ad-sh-METTL14 groups were fed a high-fat diet (20% fat and 0.25% cholesterol). Furthermore, 300 uL of constructed sh-NC or sh-METTL14 adenovirus was injected every 3 weeks into the caudal veins of mice from the Ad-sh-NC or Ad-sh-METTL14 groups, respectively. The constructed vectors were obtained from HanBio Technology Co., Ltd. (Shanghai, China). All mice were sacrificed after 24 weeks and the aortas were separated for further experiments."
item1409 "The mice were randomly divided into control, Ad-sh-NC, and Ad-sh-METTL14 groups (10 mice per group). The mice in the control group were fed a normal diet, while the Ad-sh-NC and Ad-sh-METTL14 groups were fed a high-fat diet (20% fat and 0.25% cholesterol). Furthermore, 300 uL of constructed sh-NC or sh-METTL14 adenovirus was injected every 3 weeks into the caudal veins of mice from the Ad-sh-NC or Ad-sh-METTL14 groups, respectively. The constructed vectors were obtained from HanBio Technology Co., Ltd. (Shanghai, China). All mice were sacrificed after 24 weeks and the aortas were separated for further experiments."
item1410 "Female ICR mice with 7 or 8 weeks of age were obtained. To generate the mouse model, according to our previous methods, CTX (Sigma, USA) was used at different doses (40, 80, and 120 mg/kg), CTX with 120 mg/kg was used at different time point, respectively (0, 1, 2, 4, and 8 weeks). The animals were divided into four groups: control (saline injection) and 40, 80, 120 mg/kg CTX treatment groups (n = 10 per group)."
item1411 .
item1412 "2 × 106 cells resuspended in 50 uL of Matrigel (Corning) were subcutaneously injected into 4-6 weeks old male nude mice. When tumor volumes reached 150-200 mm3, animals were divided into control group and radiotherapy group. In the radiotherapy group, tumors were treated with a single irradiation (4 Gy) when tumor volumes reached approximately 150-200 mm3. The tumor stopped growing in the next few days and then restarted growth."
item1413 "2 × 106 cells resuspended in 50 uL of Matrigel (Corning) were subcutaneously injected into 4-6 weeks old male nude mice. When tumor volumes reached 150-200 mm3, animals were divided into control group and radiotherapy group. In the radiotherapy group, tumors were treated with a single irradiation (4 Gy) when tumor volumes reached approximately 150-200 mm3. The tumor stopped growing in the next few days and then restarted growth."
item1414 "2 × 106 cells resuspended in 50 uL of Matrigel (Corning) were subcutaneously injected into 4-6 weeks old male nude mice. When tumor volumes reached 150-200 mm3, animals were divided into control group and radiotherapy group. In the radiotherapy group, tumors were treated with a single irradiation (4 Gy) when tumor volumes reached approximately 150-200 mm3. The tumor stopped growing in the next few days and then restarted growth."
item1415 "In vivo animal assay, FOXD2-AS1 overexpression promoted the tumor growth in mice subcutaneous injection."
item1416 "In vivo animal assay, FOXD2-AS1 overexpression promoted the tumor growth in mice subcutaneous injection."
item1417 "All mice had free access to food and water throughout the study. To test the oncogenic potential of FTO gene, K562 parental cells were transfected with FTO expression or control vectors for 6 h, and 5.0 × 106 transfected cells were subcutaneously injected into the bilateral flanks of the nude mice. For therapeutic experiments, K562 nilotinibR cells (1.5 × 106) were injected subcutaneously into the bilateral flanks of the nude mice. To retain the resistance phenotypes, these mice received 1 mg/kg nilotinib twice a week for 4 weeks till the tumor approached ~10 mm3. Then the tumor-bearing mice were randomly divided into groups of 3 animals and received intraperitoneal injection of either 5 mg/kg nilotinib, 10 mg/kg rhein alone or their combination in polyethylene glycol 400 (PEG400, Sigma #1546445) and saline (ratio 15:38:47), three injections each week for 5 times in total."
item1418 "Totally 5 × 106 ESCC cells after treatment were administered to randomized animals by the subcutaneous route (right flank) in 200 uL DMEM. Tumor measurement was performed every other day. At study end (at least 3 weeks), the animals underwent euthanasia, and the tumors were extracted for histology."
item1419 .
item1420 "For the xenograft tumor model, approximately 1 × 106 ccRCC cells suspended in 100 uL PBS were subcutaneously inoculated in the right flank of 5-week-old BALB/c nude mice. For the ccRCC orthotopic implantation model, approximately 1 × 106 ccRCC cells suspended in 30 uL Matrigel were injected under the renal capsule of 5-week-old BALB/c nude mice. After 6 weeks, the anesthetized mice were intraperitoneally injected with D-luciferin (Yeason) and imaged using an in vivo imaging system to detect tumor growth and metastasis. For the lung metastasis model, approximately 5 × 105 ccRCC cells suspended in PBS were injected into the tail vein of 5-week-old mice. After 6-8 weeks, mice were anesthetized and lung metastasis was imaged as above."
item1421 "For the xenograft tumor model, approximately 1 × 106 ccRCC cells suspended in 100 uL PBS were subcutaneously inoculated in the right flank of 5-week-old BALB/c nude mice. For the ccRCC orthotopic implantation model, approximately 1 × 106 ccRCC cells suspended in 30 uL Matrigel were injected under the renal capsule of 5-week-old BALB/c nude mice. After 6 weeks, the anesthetized mice were intraperitoneally injected with D-luciferin (Yeason) and imaged using an in vivo imaging system to detect tumor growth and metastasis. For the lung metastasis model, approximately 5 × 105 ccRCC cells suspended in PBS were injected into the tail vein of 5-week-old mice. After 6-8 weeks, mice were anesthetized and lung metastasis was imaged as above."
item1422 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1423 .
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item1429 .
item1430 "BALB/c nude mice (5 weeks old) were purchased from the Beijing HFK Bioscience Co. Ltd. 2×106 cells (50 uL) were mixed with 50 uL Matrigel (BD Biosciences, San Jose, CA, USA) were injected subcutaneously in the rear flank fat pad of the nude mice (N = 6, per group)."
item1431 "BALB/c nude mice (5 weeks old) were purchased from the Beijing HFK Bioscience Co. Ltd. 2×106 cells (50 uL) were mixed with 50 uL Matrigel (BD Biosciences, San Jose, CA, USA) were injected subcutaneously in the rear flank fat pad of the nude mice (N = 6, per group)."
item1432 "For the subcutaneous xenograft model, PC9 cells stably transfected with METTL3 knockdown (shMETTL3) or negative control (shNC) shRNA (5 × 106 cells per mouse, n = 6) were suspended in 200 uL PBS with 50% Matrigel matrix (Corning, USA, 354234) and then injected into one side of the axilla of nude mice."
item1433 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1434 "Conditional knockout of Fto in bone in mice was generated as previously described. Throughout the study, mice were maintained on a 12 h: 12 h light:dark cycle in a specific pathogen-free facility."
item1435 .
item1436 .
item1437 .
item1438 "A total of 3×106 RPMI8226/MM1R-Luc cells were intravenously injected into NCG mice to establish a disseminated human MM xenograft model. The in vivo antitumor effect of the FTO inhibitor MA2 combined with or without the first-line chemotherapeutic agent BTZ was evaluated as follows: 3 days post xenotransplantation, MA2 (20 mg/kg), or vehicle control was injected intraperitoneally (i.p.) daily for 10 days, and BTZ was injected intraperitoneally on days 1, 4, 8, and 11. Mouse serum was collected at specified time points during the treatment, and the tumor burden was monitored by detecting myeloma cell-secreted Lambda light chains via a Human Lambda ELISA Kit (Bethyl Laboratories, No. E88-116). Tumor development was monitored weekly after treatment with an in vivo imaging system (IVIS, SI Imaging, Lago, and LagoX). Luciferin (150 mg/kg, YEASEN, Shanghai, China) was injected intraperitoneally into the mice."
item1439 .
item1440 .
item1441 .
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item1444 .
item1445 "KOV-3 cells (1 ×106) stable transfected with METTL14 or control lentivirus, were injected subcutaneously into the right flank of BALB/c nude mice."
item1446 "For mouse xenograft tumor model, 1×106 Saos2 or HOS cells were mixed in 200 ul PBS and injected subcutaneously into 6- to 8-week-old C57BL/6 mice.umor size of mice was measured by vernier calipers every week after injection. The mice were euthanized after 28 days by intraperitoneal injection of 1% pentobarbital sodium (120 mg/kg), and the tumor tissues were removed from the mice."
item1447 Five-week-old male nude BALB/C mice were applied for this animal studies and fed with certified standard diet and tap water ad libitum in a light/dark cycle of 12 h on/12 h off.The assay was performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Stable transfection of METTL3 knockdown (sh-METTL3) or negative control (sh-blank) in SiHa cells (5 × 106 cells per 0.1 mL) were injected into the flank of mice.
item1448 Five-week-old male nude BALB/C mice were applied for this animal studies and fed with certified standard diet and tap water ad libitum in a light/dark cycle of 12 h on/12 h off.The assay was performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Stable transfection of METTL3 knockdown (sh-METTL3) or negative control (sh-blank) in SiHa cells (5 × 106 cells per 0.1 mL) were injected into the flank of mice.
item1449 .
item1450 .
item1451 .
item1452 .
item1453 "Mice were divided into two groups (n = 4/group) randomly. 3×106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
item1454 "Mice were divided into two groups (n = 4/group) randomly. 3×106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
item1455 Established subcutaneous xenografts in athymic nude mice with MIR100HGKOE4 CC-CR cells transduced with a luciferase-expressing lentiviral vector and then treated the mice with cetuximab.
item1456 .
item1457 .
item1458 .
item1459 "PC9-GR cells stably infected with KIAA1429-targeting shRNA and control were suspended in 100 uL of PBS with Matrigel matrix (BD Biosciences). Then, cells were injected into one of the flanks of BALB/c nude mice."
item1460 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
item1461 .
item1462 .
item1463 "Six-week-old male BALB/c nude mice were maintained under specific pathogen-free conditions,Mice were randomly assigned to 2 groups (N = 6). In each group, lentiviral-transduced SK-Cha-1 cells (2.5 × 106) were subcutaneously injected into the dorsal right flanks of the mice, and the mice were monitored every 3 days for tumor growth."
item1464 .
item1465 .
item1466 Established subcutaneous xenografts in athymic nude mice with MIR100HGKOE4 CC-CR cells transduced with a luciferase-expressing lentiviral vector and then treated the mice with cetuximab.
item1467 .
item1468 .
item1469 .
item1470 "Implanting 5000 human derived GSCs into the right cerebral cortex of NSG mice at a depth of 3.5 mm under a University of California, San Diego Institutional Animal Care and Use Committee (IACUC) approved protocol. Brains were harvested and fixed in 4% formaldehyde, cryopreserved in 30% sucrose, and then cryosectioned. Hematoxylin and eosin (H&E) staining was performed on sections for histological analysis. In parallel survival experiments, mice were observed until the development of neurological signs. For in vivo drug treatment studies, intracranial xenografts were generated by implanting 5000 patient-derived GSCs (387 and 4121) into the right cerebral cortex of NSG mice as described above. Mice recovered for 7 days were randomly assigned into drug vs. treatment group by a blinded investigator. Mice were then treated daily with either vehicle (25 mM Tartaric acid) or 50 mg/kg linsitinib by oral gavage."
item1471 "For induction of ESCC, 4-week-old mice were treated with drinking water containing 50 ug/mL 4NQO (Sigma-Aldrich, USA) for 16 weeks and then given normal drinking water for another 4-5 weeks. Cre was activated by the intraperitoneal injection of tamoxifen (Sigma-Aldrich, USA) at a dose of 9 mg per 40 g body weight every other day for a total of three injections. For tumor measurement, mice were sacrificed, and the esophagus was dissected immediately. The surface areas of tumors were measured as described previously."
item1472 .
item1473 .
item1474 "For subcutaneous xenograft experiments in B-NDG mice, approximately 1 × 106 MDA-MB-231 and there was subcutaneous injection of the cells that resuspended in 100 uL PBS into the left flank of the mice and were divided into 11 groups randomly (each containing 5 mice). After the treatment Atezolizumab (Selleck, Shanghai, China) or corresponding iso control antibody (Selleck, Shanghai, China) was injected intratumorally on day 3, 6, 9, 12, 15 post-MDA-MB-231 inoculations, and 5 × 106 cytokine-induced killer (CIK) cells were injected in the tail vein on day 7, 14, 21."
item1475 "For subcutaneous xenograft experiments in B-NDG mice, approximately 1 × 106 MDA-MB-231 and there was subcutaneous injection of the cells that resuspended in 100 uL PBS into the left flank of the mice and were divided into 11 groups randomly (each containing 5 mice). After the treatment Atezolizumab (Selleck, Shanghai, China) or corresponding iso control antibody (Selleck, Shanghai, China) was injected intratumorally on day 3, 6, 9, 12, 15 post-MDA-MB-231 inoculations, and 5 × 106 cytokine-induced killer (CIK) cells were injected in the tail vein on day 7, 14, 21."
item1476 .
item1477 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1478 .
item1479 .
item1480 .
item1481 .
item1482 .
item1483 .
item1484 .
item1485 .
item1486 .
item1487 .
item1488 "1×106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
item1489 .
item1490 .
item1491 .
item1492 .
item1493 .
item1494 "Liver fibrosis mice model was induced by intraperitoneal CCl4 injection for 12 weeks. The dose regimen for CCl4 in mice is 1 ml kg-1, diluted to 50% with olive oil twice per week for 12 weeks. Until 11 weeks, mice with liver-specific disruption of ALKBH5 were given hydrodynamic tail-vein injections of LV5-ALKBH5."
item1495 "Mettl3 floxed mice were purchased from GemPharmatech Co. Ltd (Nanjing, China). Pdgfr-Beta-Cre mice were purchased from Beijing Biocytogen Co. Ltd (Beijing, China) generated on C57BL/6J background. Mettl3 flox/flox mice were crossed with Pdgfr-Beta-Cre mice to generate pericyte-specific Mettl3 knockout mice. All mice were bred under the specific-pathogen free condition with free access to diet and water or their nursing mothers with alternating 12/12 light-dark cycle (lights on at 08:00 and off at 20:00)."
item1496 "Mettl3 floxed mice were purchased from GemPharmatech Co. Ltd (Nanjing, China). Pdgfr-Beta-Cre mice were purchased from Beijing Biocytogen Co. Ltd (Beijing, China) generated on C57BL/6J background. Mettl3 flox/flox mice were crossed with Pdgfr-Beta-Cre mice to generate pericyte-specific Mettl3 knockout mice. All mice were bred under the specific-pathogen free condition with free access to diet and water or their nursing mothers with alternating 12/12 light-dark cycle (lights on at 08:00 and off at 20:00)."
item1497 "Mettl3 floxed mice were purchased from GemPharmatech Co. Ltd (Nanjing, China). Pdgfr-Beta-Cre mice were purchased from Beijing Biocytogen Co. Ltd (Beijing, China) generated on C57BL/6J background. Mettl3 flox/flox mice were crossed with Pdgfr-Beta-Cre mice to generate pericyte-specific Mettl3 knockout mice. All mice were bred under the specific-pathogen free condition with free access to diet and water or their nursing mothers with alternating 12/12 light-dark cycle (lights on at 08:00 and off at 20:00)."
item1498 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
item1499 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1500 .
item1501 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
item1502 "METTL14+/- mice are generated by mating wild-type mice (C57/BL6 background) with METTL14+/- mice. METTL14+/-/APOE-/- healthy offspring mice are produced by heterozygous METTL14+/- mice and heterozygous APOE-/- mice by Mendelian ratios. APOE-/- mice and C57/BL6 mice were purchased from Model Animal Research Center of Nanjing (Nanjing, Jiangsu, China). All mice were housed in the Laboratory Animals Center of the Henan Provincial People's Hospital, with controlled temperature and humidity and a 12:12-hour dark-light cycle, and were provided water and mouse chow ad libitum."
item1503 "Mettl14-/+ mice are generated by mating wild-type mice (C57/BL6 background) with Mettl14-/+ mice. Mettl14-/+/APOE-/- healthy offspring mice are produced by heterozygous Mettl14-/+ mice and heterozygous APOE-/- mice by Mendelian ratios. APOE-/- mice and C57/BL6 mice were purchased from Model Animal Research Center of Nanjing (Nanjing, Jiangsu, China). All mice were housed in the Laboratory Animals Center of the Henan Provincial People's Hospital, with controlled temperature and humidity and a 12:12-hour dark-light cycle, and were provided water and mouse chow ad libitum."
item1504 .
item1505 .
item1506 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2×length ×0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
item1507 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2×length ×0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
item1508 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2×length ×0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
item1509 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2×length ×0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
item1510 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2×length ×0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
item1511 .
item1512 .
item1513 .
item1514 .
item1515 .
item1516 .
item1517 .
item1518 "The rats were randomly divided into a fresh air group and a COPD group and each group contained 10 rats. The COPD group was placed into a breathable cage and exposed to PM2.5 (1.46 mg/m3) from the motor vehicle exhaust for two 2-h exposure periods (from 9:00 a.m. to 11:00 a.m. and from 15:00 p.m. to 17:00 p.m.), 5 days per week, for 7 months. After each period of exposure, the animals were exposed to fresh air for 30 min to take a rest. Gasoline-powered motorcycle used as of source of the Particulate matter (PM) pollution was located next door to the rat exposure room, and the PM was directed towards the exposure room through a metal tube. Fresh air was sent to through an air pump. After exposed for 7 months, rats were performed lung function test using a Forced Pulmonary Maneuver System (Buxco Research Systems, Wilmington, NC, USA)."
item1519 "Nude mice (4 weeks, male) were used for tumor model.For the subcutaneous tumor model, 1 × 106 shNC, shWTAP or shHMBOX1 or shWTAP/shHMBOX1 U2OS cells seeded into mice via subcutaneous injection. Tumor volume and tumor weight were measured to analyze tumor growth as previous described. For orthotopic xenograft tumor model, shNC, shWTAP, shHMBOX1, or shWTAP/shHMBOX1 U2OS cells were labeled with a luminescent dye and GFP, and injected into the cavity of the tibia of mice. Thirty days after injection, tumor growth was detected. For the metastasis model, the cells were injected into the tail vein, and the lung metastasis were detected 30 days after injection using in vivo bioluminescence imaging system."
item1520 "BALB/c male nude mice were 4 weeks old. GBM cells with stable overexpression or knockdown of FTO and ovNC or shNC were transduced with lentivirus expressing luciferase. The cells were intracranially injected at a density of 5 × 105/10 uL into every mouse to form an orthotopic xenograft model. Coordinates of injection were 1 mm anterior and 2.5 mm right to the bregma, at a depth of 3.5 mm (the right frontal lobes of the mouse). Every 6 days, bioluminescence imaging (IVIS Lumina Series III; PerkinElmer, Waltham, MA) was used to image the mouse. At 8 days, we randomly chose 5 mice from each group to euthanize them, and their brain tissues were fixed with paraformaldehyde for further study. Another 5 mice were used for survival time analysis. For DB2313 (563801; MedKoo) anti-tumor research, male nude mice were subcutaneously injected with 5 × 106 U87MG cells suspended in 0.1 mL PBS. After 7 days, mice were intraperitoneally injected with DB2313 at density of 10 mg/kg/day dissolved in PBS solvent containing 10% DMSO for 7 days. The other group treated with vehicle only was set as the control group."
item1521 "BALB/c male nude mice were 4 weeks old. GBM cells with stable overexpression or knockdown of FTO and ovNC or shNC were transduced with lentivirus expressing luciferase. The cells were intracranially injected at a density of 5 × 105/10 uL into every mouse to form an orthotopic xenograft model. Coordinates of injection were 1 mm anterior and 2.5 mm right to the bregma, at a depth of 3.5 mm (the right frontal lobes of the mouse). Every 6 days, bioluminescence imaging (IVIS Lumina Series III; PerkinElmer, Waltham, MA) was used to image the mouse. At 8 days, we randomly chose 5 mice from each group to euthanize them, and their brain tissues were fixed with paraformaldehyde for further study. Another 5 mice were used for survival time analysis. For DB2313 (563801; MedKoo) anti-tumor research, male nude mice were subcutaneously injected with 5 × 106 U87MG cells suspended in 0.1 mL PBS. After 7 days, mice were intraperitoneally injected with DB2313 at density of 10 mg/kg/day dissolved in PBS solvent containing 10% DMSO for 7 days. The other group treated with vehicle only was set as the control group."
item1522 .
item1523 .
item1524 "Mettl3fl/wt mice were generated as previously described. Mettl3fl/wt mice were crossed with K14CreER mice to obtain K14creER/Mettl3fl/fl (cKO) mice. Mettl3 cKO and control mice were injected with tamoxifen and then were subjected to corneal alkali burn treatment. The right eye was the experimental eye, and the left eye was the control eye. The mice were sacrificed at 24 hours, 7 days, 14 days, 35 days, and 56 days after injury. Six mice were taken from each period. Both eyes were removed, frozen in OCT (n = 4), fixed in 4% paraformaldehyde, and embedded in conventional paraffin (n = 2)."
item1525 "Mettl3fl/wt mice were generated as previously described. Mettl3fl/wt mice were crossed with K14CreER mice to obtain K14creER/Mettl3fl/fl (cKO) mice. Mettl3 cKO and control mice were injected with tamoxifen and then were subjected to corneal alkali burn treatment. The right eye was the experimental eye, and the left eye was the control eye. The mice were sacrificed at 24 hours, 7 days, 14 days, 35 days, and 56 days after injury. Six mice were taken from each period. Both eyes were removed, frozen in OCT (n = 4), fixed in 4% paraformaldehyde, and embedded in conventional paraffin (n = 2)."
item1526 "BALB/C nude mice (5 weeks old, weighing 18-22 g) were fed in specific pathogen-free facilities and subcutaneously inoculated with U266 cells (1 × 106). The mice were randomly divided into 3 groups with 6 mice per group, when the tumor was measurable. Then, miR-27a-3p mimic or sh-METTL3 was injected intratumorally at an interval of 4 days a total of 4 times. Tumor volume was measured using a digital caliper every week and calculated using the formula V = 1/2 (width2 × length)."
item1527 "BALB/C nude mice (5 weeks old, weighing 18-22 g) were fed in specific pathogen-free facilities and subcutaneously inoculated with U266 cells (1 × 106). The mice were randomly divided into 3 groups with 6 mice per group, when the tumor was measurable. Then, miR-27a-3p mimic or sh-METTL3 was injected intratumorally at an interval of 4 days a total of 4 times. Tumor volume was measured using a digital caliper every week and calculated using the formula V = 1/2 (width2 × length)."
item1528 .
item1529 .
item1530 .
item1531 .
item1532 .
item1533 .
item1534 .
item1535 .
item1536 .
item1537 .
item1538 .
item1539 "Mettl14 heterozygous mice (Mettl14-/+) were established from C57/BL6 mice by Cyagen Biosciences, Inc. (Suzhou, Jiangsu, China), using CRISPR/Cas9-based targeting and homology-directed repair. C57/BL6 and APOE-/- mice were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Mettl14-/+APOE-/- mice were generated by breeding Mettl14-/+ mice with APOE-/- mice. Eight- to 10-week-old male APOE-/- (WT) mice and Mettl14-/+APOE-/- (KO) mice were fed a high-cholesterol diet (D12108C, Opensource diets) for 12 weeks. Then, the mice were euthanized for further analysis."
item1540 "Mettl14 heterozygous mice (Mettl14-/+) were established from C57/BL6 mice by Cyagen Biosciences, Inc. (Suzhou, Jiangsu, China), using CRISPR/Cas9-based targeting and homology-directed repair. C57/BL6 and APOE-/- mice were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Mettl14-/+APOE-/- mice were generated by breeding Mettl14-/+ mice with APOE-/- mice. Eight- to 10-week-old male APOE-/- (WT) mice and Mettl14-/+APOE-/- (KO) mice were fed a high-cholesterol diet (D12108C, Opensource diets) for 12 weeks. Then, the mice were euthanized for further analysis."
item1541 .
item1542 "Male C57BL/6J mice (6 weeks old) were given drinking water containing 0.05% (w/v) BBN (TCI, catalog no. B0938) for 20 weeks. After the BBN administration, mice were given normal drinking water and injected with 10% DMSO (as a control) or 20 mg/kg SP600125 (Selleck, catalog no. 129-56-6) i.p. every 3 days. After seven injections, mice were euthanized for tissue retrieval. For the bladder cancer cell-derived xenograft mouse model, male C57BL/6J mice (6 weeks old) were injected subcutaneously with 1 × 106 MB49 cells. One week after bladder cancer cell injection, 10% DMSO or 20 mg/kg SP600125 were injected i.p. every 3 days."
item1543 "Approximately 5×106 normal H1299 cells or stable YTHDC2-overexpressing H1299 cells were implanted subcutaneously into the right flank of the animals (n=8 mice per group). Animals were euthanized by cervical dislocation ~30 days after implantation, and tumors were collected and photographed."
item1544 .
item1545 .
item1546 "2 × 107 PARP inhibitor resistant PEO1 cells were suspended in 200 uL PBS : Matrigel (1:1) unilaterally injected subcutaneously into the right dorsal flank of 6-8 week-old female immunocompromised non-obese diabetic/severe combined immunodeficiency (NOD/SCID) gamma (NSG) mice. When the average tumor size reached ~100 mm3, the mice were then randomized into four groups and treated with vehicle control, Olaparib (50 mg/kg), XAV939 (5 mg/kg) or a combination daily for 18 days."
item1547 "2 × 107 PARP inhibitor resistant PEO1 cells were suspended in 200 uL PBS : Matrigel (1:1) unilaterally injected subcutaneously into the right dorsal flank of 6-8 week-old female immunocompromised non-obese diabetic/severe combined immunodeficiency (NOD/SCID) gamma (NSG) mice. When the average tumor size reached ~100 mm3, the mice were then randomized into four groups and treated with vehicle control, Olaparib (50 mg/kg), XAV939 (5 mg/kg) or a combination daily for 18 days."
item1548 "An incision was made in the skin along the medial dorsal line to the aponeurotic and muscular planes, and the posterior vertebral arches were exposed from T8 to T12. Under the dissection stereomicroscope, 3-mm-long laminectomy was performed on the caudal end of T10 vertebra and the rostral end of T11 vertebra. The Infinite Horizons impactor (Infinite Horizons, L.L.C., Lexington, KY, USA) was adopted to produce the contusion SCI using a force of 60 kdyn/cm2. The SCI model rats were established and randomly assigned to SCI model group, ant-NC (negative control, SCI rats treated with lentiviral (lv)-shRNA NC of Mettl14) group and ant-Mettl14 group (SCI rats treated with lv-shRNA of Mettl14). Rats were subjected to laminectomy and then treated with lv-shRNA Mettl14/lv-shRNA-NC (50 ul/day, 100 nmoL/mL; RiboBio, Guangzhou, China) via an intrathecal injection through lumbar puncture for 3 days (0, 1, and 2 days) after 15 min of SCI modelling. In addition, the unmodeled rats were set as sham group."
item1549 "An incision was made in the skin along the medial dorsal line to the aponeurotic and muscular planes, and the posterior vertebral arches were exposed from T8 to T12. Under the dissection stereomicroscope, 3-mm-long laminectomy was performed on the caudal end of T10 vertebra and the rostral end of T11 vertebra. The Infinite Horizons impactor (Infinite Horizons, L.L.C., Lexington, KY, USA) was adopted to produce the contusion SCI using a force of 60 kdyn/cm2. The SCI model rats were established and randomly assigned to SCI model group, ant-NC (negative control, SCI rats treated with lentiviral (lv)-shRNA NC of Mettl14) group and ant-Mettl14 group (SCI rats treated with lv-shRNA of Mettl14). Rats were subjected to laminectomy and then treated with lv-shRNA Mettl14/lv-shRNA-NC (50 ul/day, 100 nmoL/mL; RiboBio, Guangzhou, China) via an intrathecal injection through lumbar puncture for 3 days (0, 1, and 2 days) after 15 min of SCI modelling. In addition, the unmodeled rats were set as sham group."
item1550 .
item1551 "For induction of BCa, 6-8-week-old mice were treated with drinking water containing 500 ug/ml BBN for 16 weeks and then given normal water for another 10 weeks. Tamoxifen was intraperitonelly injected to the mice with 0.08 mg/g of body weight each day for 3 days in order to inductively knock out the target gene."
item1552 "For induction of BCa, 6-8-week-old mice were treated with drinking water containing 500 ug/ml BBN for 16 weeks and then given normal water for another 10 weeks. Tamoxifen was intraperitonelly injected to the mice with 0.08 mg/g of body weight each day for 3 days in order to inductively knock out the target gene."
item1553 "They were subcutaneously and caudal vein injected with YTHDC2 knockout AGS cells, respectively. After 7 weeks, the mice were sacrificed and tumor size and lung metastasis nodules were recorded."
item1554 "A549 cells were transfected with lentivirus-packaged sh-HNRNPA2B1 (lv-sh-HNRNPA2B1) or control (lv-shCtrl). Then, each mouse was injected subcutaneously with A549 cells of indicated transfection group to generate xenografts. The tumor volume ((width2 × length)/2) was evaluated 4 days a time until 28 days."
item1555 .
item1556 "For the subcutaneous transplantation model, 100 uL of 1 × 106 cells were injected subcutaneously into the right armpit of BALB/c nude mice. Animal weight and tumor diameter were measured once a week from the time of implantation.For the pancreatic cancer orthotopic implantation model, 200 uL of Panc02-lucifer cells (2 × 107) were injected into the pancreas in mice anesthetized and laparotomized. After 4 weeks, the mice were anesthetized and injected with 150 mg/kg d-luciferin, via the tail vein.For the liver metastasis model, BALB/c nude mice received 2 × 106 cells (in 100 uL DMEM), directly injected into the spleen. Their body weight was measured once a week from the time of implantation."
item1557 .
item1558 "After 7 days of acclimatization, STZ, dissolved in 0.1 M citrate buffer, was intraperitoneally administered daily to mice at a dose of 50 mg/kg after 12 h of food deprivation each day for 5 consecutive days. The type 1 mice diabetic mice were randomly separated into four groups (n = 5-8): AAV9-scramble-control group; AAV9-scramble-STZ; AAV9-shRNA-control; and AAV9-shRNA-STZ group (Hanbio Biotechnology, China). The 50 UL titer of 1 × 1012 virus was injected into the renal pelvis using an insulin needle and maintained there for 2 to 3 s. The type 2 diabetic mice were randomly separated into four groups (n = 5-8): AAV9-scramble-db/m group; AAV9-scramble-db/db group; AAV9-shRNA-db/m group; and AAV9-shRNA-db/db group (Hanbio Biotechnology, China). The 100 UL titer of 1 × 1012 virus was injected into the tail vein using an insulin needle and maintained there for 2 to 3 s. Blood glucose was monitored weekly in mice. At the end of 12 weeks, the 24-h urine samples were collected from the mice kept in metabolic cages."
item1559 A total of 40 BALB/c nude mice were chosen and assigned to two groups: shCtrl group (injected with HepG2 cells) and shIGF2BP2 group (injected with HepG2 cells with IGF2BP2 knockdown). 200 ul of the above cell suspension containing 2 × 105 cells was injected into the left or right back of each mice.
item1560 "Each group included 3 mice. 1.0 × 106 stably transfected ACHN cells in 150 uL were injected into a tail vein of each mouse, 45 days after which lungs were excised from the sacrificed mice and stained by Hematoxylin and Eosin (HE) Staining."
item1561 "About 1 × 107 stable METTL3 overexpression and negative control SUM-1315 cells were injected subcutaneously into the axilla of the female BALB/C nude mice (4-6 weeks old, 18-20 g, 10 mice/group). One week after injection, the two groups, METTL3 OE and NC, were then randomly allocated into the control group and experimental group (5 mice/group), which were treated with PBS or metformin (250 mg/kg/dose, respectively). PBS or metformin was administered every 2 days via intraperitoneal injection."
item1562 "Male C57BL/6J mice were anesthetized with an intraperitoneal injection of pentobarbital (40 mg/kg). The abdominal aorta between the renal arteries and the bifurcation of the iliac arteries was disassociated from the surrounding structures. Video microscopy was used to assay the diameter of the aorta in triplicate. After the measurements were taken, a small piece of gauze dipped in 0.5 mol/L CaCl2 was spread perivascularly onto the aortic passage for 15 min. Control mice received substitute treatment with NaCl (0.9%)-soaked gauze for 15 min. Then, the aorta was rinsed with 0.9% sterile saline, and the incision was sutured. After 3 or 6 weeks, all the animals were sacrificed, and the aortas were harvested for further analysis."
item1563 "The mice were divided into two groups with three mice in each group and their flank was subcutaneously injected with 1 × 107 OS cells. 28 days following subcutaneous injection, the mice were sacrificed through carbon dioxide euthanasia (30%/min) to obtain tumor weight and volume measurements."
item1564 "Eighteen BALB/C female nude mice aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml. The prepared cell suspension was injected into the left armpit of the mice, and the subsequent tumor growth was recorded."
item1565 "Eighteen BALB/C female nude mice aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml. The prepared cell suspension was injected into the left armpit of the mice, and the subsequent tumor growth was recorded."
item1566 .
item1567 "Eighteen BALB/C female nude mice aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml. The prepared cell suspension was injected into the left armpit of the mice, and the subsequent tumor growth was recorded."
item1568 "5.0 × 106 SW480 cells (infected with scr or KIAA1429 shRNA) that suspended in 50 ul PBS and mixed with an equal volume of matrigel were subcutaneously injected in a 6-weeks-old male NOD/SCID (The Jackson Laboratory, Stock No: 001303) mice flank. We started measuring tumor size at the indicated times one week after injection."
item1569 "Mettl5 +/- mice with C57BL/6N background were generated by using CRISPR-Cas9 systems. The exon 2, exon 3 and exon 4 of Mettl5 were deleted in the knockout Mettl5 allele. Mice were genotyped for the targeted allele by PCR using tail DNA."
item1570 "Mice were anesthetized with 0.3% sodium pentobarbital (75 mg×kg-1) intraperitoneally, and the aortic arch was tied with a 6-0 nylon suture between the brachiocephalic and left common artery with a homemade L-shaped 26G cushion needle. After ligation, the needle was quickly removed, and the skin was closed. The sham operation was identical, except that the thread was not ligated. Moreover, mice were injected with rAAV9 (4 × 1011 vector genomes (vg)/mouse) carrying an empty vector, YTHDF2 or YTH-del via the tail vein."
item1571 .
item1572 HCC-LM3 cells transfected with sh-NC and sh-RBM15B-3 were injected into the axilla or tail vein of mice.
item1573 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
item1574 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
item1575 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
item1576 .
item1577 .
item1578 .
item1579 .
item1580 .
item1581 "Once the tumor volume increased to about 1 cm3, six groups of MCF7 bearing mice (n = 10 in each group) were injected with PBS (0.1 ml, caudal vein) and adriamycin (0.1 ml, 10 mg/kg), respectively. When the tumor reached 1.5 cm in any direction (defined as event-free survival analysis), 10 mice in each group were selected to measure the tumor size and weight on the 12th day after adriamycin injection."
item1582 "Once the tumor volume increased to about 1 cm3, six groups of MCF7 bearing mice (n = 10 in each group) were injected with PBS (0.1 ml, caudal vein) and adriamycin (0.1 ml, 10 mg/kg), respectively. When the tumor reached 1.5 cm in any direction (defined as event-free survival analysis), 10 mice in each group were selected to measure the tumor size and weight on the 12th day after adriamycin injection."
item1583 "Once the tumor volume increased to about 1 cm3, six groups of MCF7 bearing mice (n = 10 in each group) were injected with PBS (0.1 ml, caudal vein) and adriamycin (0.1 ml, 10 mg/kg), respectively. When the tumor reached 1.5 cm in any direction (defined as event-free survival analysis), 10 mice in each group were selected to measure the tumor size and weight on the 12th day after adriamycin injection."
item1584 "Six-week-old, female SCID-Berge mice were purchased from Vitalriver. EC cells with IGF2BP1 overexpression or silencing or the appropriate controls (1×106) were injected into lower abdominal cavity of each mouse (n = 5 mice/group)."
item1585 .
item1586 "The adeno-associated viruses (AAV) that could silence METTL3 (sh-METTL3) and the negative control adeno-associated viruses (sh-NC) were obtained from WZ Biosciences Inc. (Jinan, China). APOE-/- mice were randomly divided into AS + sh-NC and AS + sh-METTL3 groups. Each group contains five mice. Mice were fed with the standard diet for 1 week to acclimatize. After 1 week of acclimation, mice were challenged with a high-fat and high-cholesterol feed H10540 (Beijing HFK BIOSCIENCE Co., Ltd., Beijing, China). The formula of the H10540 feed was shown in Supplementary File S1. After 8 weeks of HFD feeding, sh-NC or sh-METTL3 adeno-associated virus serotype 9 (AAV9, 1012 viral genome copies per mouse) were respectively delivered into mice in AS + sh-NC or AS + sh-METTL3 group through tail vein injection. At 14 weeks after HDF feeding, mice fasted overnight."
item1587 "The adeno-associated viruses (AAV) that could silence METTL3 (sh-METTL3) and the negative control adeno-associated viruses (sh-NC) were obtained from WZ Biosciences Inc. (Jinan, China). APOE-/- mice were randomly divided into AS + sh-NC and AS + sh-METTL3 groups. Each group contains five mice. Mice were fed with the standard diet for 1 week to acclimatize. After 1 week of acclimation, mice were challenged with a high-fat and high-cholesterol feed H10540 (Beijing HFK BIOSCIENCE Co., Ltd., Beijing, China). The formula of the H10540 feed was shown in Supplementary File S1. After 8 weeks of HFD feeding, sh-NC or sh-METTL3 adeno-associated virus serotype 9 (AAV9, 1012 viral genome copies per mouse) were respectively delivered into mice in AS + sh-NC or AS + sh-METTL3 group through tail vein injection. At 14 weeks after HDF feeding, mice fasted overnight."
item1588 .
item1589 "The adeno-associated viruses (AAV) that could silence METTL3 (sh-METTL3) and the negative control adeno-associated viruses (sh-NC) were obtained from WZ Biosciences Inc. (Jinan, China). APOE-/- mice were randomly divided into AS + sh-NC and AS + sh-METTL3 groups. Each group contains five mice. Mice were fed with the standard diet for 1 week to acclimatize. After 1 week of acclimation, mice were challenged with a high-fat and high-cholesterol feed H10540 (Beijing HFK BIOSCIENCE Co., Ltd., Beijing, China). The formula of the H10540 feed was shown in Supplementary File S1. After 8 weeks of HFD feeding, sh-NC or sh-METTL3 adeno-associated virus serotype 9 (AAV9, 1012 viral genome copies per mouse) were respectively delivered into mice in AS + sh-NC or AS + sh-METTL3 group through tail vein injection. At 14 weeks after HDF feeding, mice fasted overnight."
item1590 "The adeno-associated viruses (AAV) that could silence METTL3 (sh-METTL3) and the negative control adeno-associated viruses (sh-NC) were obtained from WZ Biosciences Inc. (Jinan, China). APOE-/- mice were randomly divided into AS + sh-NC and AS + sh-METTL3 groups. Each group contains five mice. Mice were fed with the standard diet for 1 week to acclimatize. After 1 week of acclimation, mice were challenged with a high-fat and high-cholesterol feed H10540 (Beijing HFK BIOSCIENCE Co., Ltd., Beijing, China). The formula of the H10540 feed was shown in Supplementary File S1. After 8 weeks of HFD feeding, sh-NC or sh-METTL3 adeno-associated virus serotype 9 (AAV9, 1012 viral genome copies per mouse) were respectively delivered into mice in AS + sh-NC or AS + sh-METTL3 group through tail vein injection. At 14 weeks after HDF feeding, mice fasted overnight."
item1591 "1 × 106 cells in 100 uL PBS (shMETTL3-1 or shNC) were respectively injected into each mouse through the tail vein. Pulmonary metastases were monitored after fourteen days using the imaging system (IVIS) Spectrum (PerkinElmer, USA)."
item1592 "After anesthesia (50 mg/kg pentobarbital sodium, intraperitoneal injection), the left thorax was cut to expose the heart, and the left anterior descending (LAD) coronary artery was ligated by 7/0 sterile suture. Myocardial ischemia was induced by 30 min of LAD coronary artery ligation and following 2 h of reperfusion. Sham group mice underwent the same surgical procedure without LAD coronary artery ligation."
item1593 "To construct the subcutaneous tumorigenesis model, the cells were suspended in 100 uL of PBS and Matrigel matrix (BD Biosciences, USA) (1:1) and injected into the right flanks of 6-week-old female BALB/c nude mice.To construct the lymph node metastasis model, we injected 1 × 105/50 uL stably infected SCC9 cells into the left hind footpads of BALB/c mice."
item1594 "To construct the subcutaneous tumorigenesis model, the cells were suspended in 100 uL of PBS and Matrigel matrix (BD Biosciences, USA) (1:1) and injected into the right flanks of 6-week-old female BALB/c nude mice.To construct the lymph node metastasis model, we injected 1 × 105/50 uL stably infected SCC9 cells into the left hind footpads of BALB/c mice."
item1595 "Ten four-week-old BALB/c nude mice were injected with LINC00958-overexpressing or vector-transfected cells. Briefly, 5 × 106 cells were subcutaneously injected in the flank of mice. Four weeks after injection, the mice were sacrificed and examined by weighting."
item1596 "The right flanks of mice were injected subcutaneously with 2 × 106 MiaPaCa-2 cells stably expressing shFTO and a scrambled shRNA in 100 uL PBS. Tumors were measured using an external caliper once per week, and tumor volume was calculated with the formula: (length × width2)/2."
item1597 "The right flanks of mice were injected subcutaneously with 2 × 106 MiaPaCa-2 cells stably expressing shFTO and a scrambled shRNA in 100 uL PBS. Tumors were measured using an external caliper once per week, and tumor volume was calculated with the formula: (length × width2)/2."
item1598 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5×106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
item1599 .
item1600 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5×106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
item1601 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
item1602 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
item1603 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
item1604 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
item1605 "After the animal was anesthetized with isoflurane, a midline incision in the lower lumbar back region was made and the lumbar articular process was exposed and then removed. The exposed DRG was injected with viral solution (1-1.5 ul in rats and 0.5-1 ul in mice) through a glass micropipette connected to a Hamilton syringe. The pipette was retained for 10 min after injection. Animals showing signs of paresis or other abnormalities were excluded. The injected DRGs were stained with hematoxylin/eosin to examine the integrity of their structure and whether they contained visible leukocytes."
item1606 .
item1607 "HCT-116 cells (3 × 105 cells in 200 uL of saline) were subcutaneously injected into the nude mice to establish xenograft tumors. After 10 days, 10 mg/kg OX or saline was intraperitoneally injected (n = 5 for each group). Si-METTL3 or si-TRAF5 (10 nmol/20 g body weight) was injected twice intratumorally before the start of OX treatment. The mice were examined every 2 days and sacrificed 4 weeks after the OX treatment."
item1608 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1609 "OP rats were fixed prone, the skin was prepared and the skull was disinfected, resulting in a full-thickness defect of 8mm. BCP incubated with OP-BMSCs was implanted in the skull defect area. After 8 weeks, skull specimens were taken after the rats were euthanized."
item1610 "For the mouse model of miR-320a-3p overexpression, a total of 24 male C57BL/6 mice were divided randomly into four groups (n = 6 in each group): saline, silica, silica plus AAV9-miR-NC, and silica plus AAV9-miR-320a-3p. The mice in the silica plus AAV9-miR-NC/AAV9-miR-320a-3p groups were anesthetized using the same method, then administered intratracheally 50 uL AAV9-miR-NC/AAV9-miR-320a-3p per mouse at a titer of 8 × 1012 v. g./ml. Three weeks later, these mice were treated in the same way using anesthesia, saline, and silica as mentioned above. Subsequently, after 4 weeks, the mice were sacrificed, and the lungs were isolated and frozen at -80 ℃ for further study."
item1611 "For the mouse model of miR-320a-3p overexpression, a total of 24 male C57BL/6 mice were divided randomly into four groups (n = 6 in each group): saline, silica, silica plus AAV9-miR-NC, and silica plus AAV9-miR-320a-3p. The mice in the silica plus AAV9-miR-NC/AAV9-miR-320a-3p groups were anesthetized using the same method, then administered intratracheally 50 uL AAV9-miR-NC/AAV9-miR-320a-3p per mouse at a titer of 8 × 1012 v. g./ml. Three weeks later, these mice were treated in the same way using anesthesia, saline, and silica as mentioned above. Subsequently, after 4 weeks, the mice were sacrificed, and the lungs were isolated and frozen at -80 ℃ for further study."
item1612 .
item1613 "ICC tumor cells (LIPF178c-shCtrl/shALKBH5) of 5 × 106 were injected into the right flank of NCG mice. Tumor volume was calculated by the formula: volume = ab2/2 (a, the longer axis; b, the shorter axis). T-cell killing assay in vitro was conducted as previously reported (20). PBMCs from healthy donors were activated and expanded as described above. The day before tumor cell injection, PBMC (i.v. 1 × 107 cells) was adoptively transferred to NCG mice via the tail vein. At the end, the PBMC was isolated and subjected to flow cytometry for detecting T-cell percentage."
item1614 .
item1615 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
item1616 "Before the tests, animals were fasted for 8 h. l glucose tolerance test (GTT) was conducted during week 11 on the diet. The mice were challenged with 2 g/kg body weight d-glucose (Sigma-Aldrich, USA). Insulin tolerance test (ITT) was conducted during week 12 on the diet. For insulin tolerance test, mice were injected intraperitoneally with 0.75 U/kg body weight insulin (Sigma-Aldrich, USA). For both tests, blood samples were taken from the tail vein and glucose levels were measured at indicated time points after administration using an AlphaTRAK glucometer."
item1617 "Before the tests, animals were fasted for 8 h. l glucose tolerance test (GTT) was conducted during week 11 on the diet. The mice were challenged with 2 g/kg body weight d-glucose (Sigma-Aldrich, USA). Insulin tolerance test (ITT) was conducted during week 12 on the diet. For insulin tolerance test, mice were injected intraperitoneally with 0.75 U/kg body weight insulin (Sigma-Aldrich, USA). For both tests, blood samples were taken from the tail vein and glucose levels were measured at indicated time points after administration using an AlphaTRAK glucometer."
item1618 "H1299 cells were transfected with sh-ABHD11-AS1 and harvested from six-well plates, then resuspended at a density 1 × 107 cells/ml. Mice were subsequently injected with 100 uL suspension at the right flank. After injection, tumor weight and length were examined every 3 days."
item1619 "Mice were randomized into three groups (n = 7/group), 1 × 107 PC9 cells absorbed exosomes were subcutaneously injected into the Bilateral groin of mice. Treatment began 1 week following injection, the mice were intraperitoneally injected with gefitinib (30 mg/kg/day)."
item1620 .
item1621 "Mice were randomized into three groups (n = 7/group), 1 × 107 PC9 cells absorbed exosomes were subcutaneously injected into the Bilateral groin of mice. Treatment began 1 week following injection, the mice were intraperitoneally injected with gefitinib (30 mg/kg/day)."
item1622 .
item1623 "To construct the metastasis model, 5 × 106 FaDu cells were transfected with sh-IGF2BP2-luc and sh-NC-luc, suspended in 60 uL PBS, and then injected into the footpads of the mice. Six weeks after injection, mice were subjected to bioluminescence imaging to evaluate lymphatic metastasis. For bioluminescence imaging, mice were anesthetized by inhaling 2% isoflurane for approximately 5 min, injected intraperitoneally with D-Luciferin potassium salt (200 uL, 150 ug/ml, ST196, Beyotime, Shanghai, China), and imaged with a bioluminescence system (NightOwl II LB983, Berthold Technologies, Germany)."
item1624 "Approximately 5 × 106 control and ILF3-AS1 silencing Huh7 cells were subcutaneously implanted into the right flank of nude mice.Xenograft size was measured every 7 days and calculated using the equation V(mm3)=(length×width2)/2. 35 days later, the mice were sacrificed, and the tumor tissues were isolated and weighed."
item1625 "Approximately 5 × 106 control and ILF3-AS1 silencing Huh7 cells were subcutaneously implanted into the right flank of nude mice.Xenograft size was measured every 7 days and calculated using the equation V(mm3)=(length×width2)/2. 35 days later, the mice were sacrificed, and the tumor tissues were isolated and weighed."
item1626 .
item1627 "For the tumor growth analysis, AGS cells were subcutaneously injected into nude mice, and then the tumor volumes were monitored every 5 days. Tumor volumes were estimated based on the length and width and calculated using the following formula: tumor volume = (length × width2)/2. About 1 month later, the nude mice were sacrificed, and then tumors were excised, pictured, and weighed. For the tumor metastasis analysis, AGS cells were injected into nude mice by Tail Vein. About 1 month later, the nude mice were sacrificed, and then lung with metastasis lesions were excised, pictured, and counted."
item1628 .
item1629 "For the xenograft model, METTL3 stable overexpressed SW620 cells (1 × 107) or control cells were subcutaneously injected into the right axilla of the female anesthetized BALB/C nude mice (4-6 weeks old, 18-20 g, four mice per group), respectively. The body weight and tumor volumes (length × width2 × 0.5) were measured twice a week. After 21 days, all mice were sacrificed and tumors were surgically removed for hematoxylin-eosin (H&E) staining.For the metastasis model, MTTL3 stable overexpressed SW620 cells (1 × 106) or control cells were injected into the exposed spleen of the anesthetized BALB/C nude mice, respectively. After 21 days, liver metastases were carefully detected using a fluorescent stereoscope and embedded for H&E staining."
item1630 "For the xenograft model, METTL3 stable overexpressed SW620 cells (1 × 107) or control cells were subcutaneously injected into the right axilla of the female anesthetized BALB/C nude mice (4-6 weeks old, 18-20 g, four mice per group), respectively. The body weight and tumor volumes (length × width2 × 0.5) were measured twice a week. After 21 days, all mice were sacrificed and tumors were surgically removed for hematoxylin-eosin (H&E) staining.For the metastasis model, MTTL3 stable overexpressed SW620 cells (1 × 106) or control cells were injected into the exposed spleen of the anesthetized BALB/C nude mice, respectively. After 21 days, liver metastases were carefully detected using a fluorescent stereoscope and embedded for H&E staining."
item1631 .
item1632 .
item1633 .
item1634 .
item1635 "HL-60 cells (1 × 107) suspended in 0.1 ml PBS containing 50% Matrigel were subcutaneously injected into the flanks of the mice. When tumor sizes reached 200 mm3, the mice were randomly distributed into four groups with the indicated dosages of saline, cytarabine and BP alone or in combination. For BP injections, the solution was delivered intraperitoneally at 106 ug/kg body weight for the first 8 consecutive days. For cytarabine injections, the solution was delivered intraperitoneally at 100 mg/kg body weight three times (once every three days). The combination group was administered intraperitoneally three times (once every three days) with the same dosages as described above. The control group was treated with an equivalent amount of saline."
item1636 "HL-60 cells (1 × 107) suspended in 0.1 ml PBS containing 50% Matrigel were subcutaneously injected into the flanks of the mice. When tumor sizes reached 200 mm3, the mice were randomly distributed into four groups with the indicated dosages of saline, cytarabine and BP alone or in combination. For BP injections, the solution was delivered intraperitoneally at 106 ug/kg body weight for the first 8 consecutive days. For cytarabine injections, the solution was delivered intraperitoneally at 100 mg/kg body weight three times (once every three days). The combination group was administered intraperitoneally three times (once every three days) with the same dosages as described above. The control group was treated with an equivalent amount of saline."
item1637 Mettl14f/f mice were generated as previously described with CRISPR-Cas9 technology by insertion of two loxp sites into Mettl14 genome loci. Mettl14f/f mice without Villin-Cre were used as WT controls (Mettl14 WT) for Mettl14 KO mice. Mettl14f/f mice were crossed with Lgr5-eGFP-IRES-creERT2 (Lgr5-Cre) mice to generate Mettl14 depletion in Lgr5+ stem cells.
item1638 "For the lung metastasis model, stably transfected HepG2 cells (1 × 106/0.1 mL DMEM) were injected into each nude mouse through the tail vein. Five weeks later, mice were euthanized, and the lung tissues were collected."
item1639 "For the lung metastasis model, stably transfected HepG2 cells (1 × 106/0.1 mL DMEM) were injected into each nude mouse through the tail vein. Five weeks later, mice were euthanized, and the lung tissues were collected."
item1640 "For the lung metastasis model, stably transfected HepG2 cells (1 × 106/0.1 mL DMEM) were injected into each nude mouse through the tail vein. Five weeks later, mice were euthanized, and the lung tissues were collected."
item1641 "Six-week-old nude mice were randomly divided into two groups (three mice per group) and cultured with continuous access to sterile food and water in pathogen-free sterile conditions. To establish the OSCC xenograft model, we subcutaneously injected 5 × 106 SCC-9 cells stably transfected with METTL3 shRNA or sh-NC vectors into nude mice."
item1642 .
item1643 "Six-week-old nude mice were randomly divided into two groups (three mice per group) and cultured with continuous access to sterile food and water in pathogen-free sterile conditions. To establish the OSCC xenograft model, we subcutaneously injected 5 × 106 SCC-9 cells stably transfected with METTL3 shRNA or sh-NC vectors into nude mice."
item1644 "In vivo, the group of HCT116 cells labeled with luciferase were surgically injected into the spleen of nude mice. After 2 months, the nude mice were subjected to D-luciferin injection and the luciferase signals were monitored and quantified."
item1645 .
item1646 .
item1647 "Mice were anesthetized with Nembutal. The lower back was dissected until the transverse lumbar process was exposed. After the process was removed, the underneath L4 spinal nerve was ligated with a silk 6-0 thread. A slight distal location was chosen for transection around the ligation site. Subsequent layers of muscle and skin were closed. The sham groups undertook identical procedures, but without the transection or ligature of the corresponding nerve. The intraspinal injection was performed as described previously. In short, after anesthetized with Nembutal, mice underwent hemilaminectomy at the L1-L2 vertebral segments. The intraspinal injection was carried out ipsilaterally on the left side. By using a glass micropipette, each animal received two injections (5 × 105 TU per injection, 0.8 mm from the midline, 0.5 mm apart in rostrocaudal axis, 0.5 mm deep) of lentivirus following the L3-L4 dorsal root entry zone after exposure of spinal cord. The tip of glass micropipette should reach a depth of lamina II-IV of the spinal cord. Finally, the dorsal muscle and skin were sutured layer by layer."
item1648 "U2OS cells with stable METTL3 expression were screened using puromycin for subsequent experiments. U2OS cells (1 × 106) were injected subcutaneously to the right side of each mouse (the mice were numbered according to their weight, and the experimenters randomly divided the nude mice into 12 groups by the random number method, with 12 mice in each group). The status of mice was detected every 2 days. Tumor growth and volume were measured once a week.Tumor volume was measured: Volume = Width2 × Length/2. On the 35th day after the operation, nude mice were euthanized by intraperitoneal injection of ≥100 mg/kg pentobarbital sodium. Tumor resection was performed and tumor weight was recorded. Weight loss >10% of the body weight or the maximum diameter of the tumor >1.5 cm was the humane end point."
item1649 .
item1650 .
item1651 .
item1652 .
item1653 .
item1654 "For the tumorigenicity studies, 3 × 106 HCC827 cells stably expressing sh-LINC01833 or sh-NC were injected subcutaneously into the ventral side of male BALB/c nude mice in the according groups with five mice in each group. Five mice were sampled each time. Tumor size and weight were examined at 28 days after injection."
item1655 "The model mice were intraperitoneally injected with streptozotocin (STZ; Sigma-Aldrich Corp., USA). The dose of STZ was 50 mg/kg for 5 days. 7 days after the last injection, blood glucose concentrations were recorded. Mouse models of diabetes were considered established when fasting blood glucose concentrations reached >11.1 mmol/L, and body weight was measured."
item1656 .
item1657 "Male nu/nu mice between 4 and 6 weeks of age received subcutaneous injections of equivalent Hep3B cells expressing either LV-shMETTL3 or LV-USP7 within 30 min of harvesting on the right and left flanks. The tumor was weighed after approximately 4 weeks, and the volume was measured every 5 days."
item1658 "For the proliferation assays, C4-2B cells of KIF3C knockdown, negative control (1×106/200uL) were subcutaneously injected into BALB/c nude mice. The tumors were dissected and weighed (4-6 weeks old, male)."
item1659 .
item1660 "Xenograft mouse model was used to verify the tumorigenic effect of METTL16 in vivo. BALB/c nude mice (4 weeks old) were injected with METTL16 gene knock-down stable MGC803 GC cells (3 × 106 cells/mice, subcutaneous injection) or shNC control cells (3 × 106, subcutaneous injection), and the dose was 100 uL, with PBS as solvent. The tumour size was measured every 3-5 days. At the end of feeding (6 weeks after subcutaneous injection), the mice were killed and the tumours were extracted for histological analysis."
item1661 .
item1662 "C57BL/6 mouse hearts were subjected to ischemia/reperfusion (I/R) in vivo as described previously (Bock-Marquette et al., 2004; Song et al., 2015; Brocard et al., 2017). I/R injury in mice was induced by 45-min ischemia, followed by 7-day and 4-week reperfusion in a loss-of-function study (Figure 1) and gain-of-function study (Figure 2), respectively. In brief, mice were anesthetized with 2% avertin (0.1 ml/10g body weight; Sigma-Aldrich Corporation, United States) through intraperitoneal injection. To generate I/R injury, the left anterior descending coronary artery (LAD) was ligated with 7-0 nylon for 45 min and then was removed. For the sham group, a suture was passed under the LAD but without ligation. According to the experimental requirements, at different time points of cardiac I/R, the mice were anesthetized for assessing heart function by echocardiographic measurement. All the mice survived during the process of I/R injury after the operation."
item1663 Suspension of H1299 cells (5.0 × 105) was subcutaneously injected into the right flanks of the mice.
item1664 "C57BL/6 mouse hearts were subjected to ischemia/reperfusion (I/R) in vivo as described previously (Bock-Marquette et al., 2004; Song et al., 2015; Brocard et al., 2017). I/R injury in mice was induced by 45-min ischemia, followed by 7-day and 4-week reperfusion in a loss-of-function study (Figure 1) and gain-of-function study (Figure 2), respectively. In brief, mice were anesthetized with 2% avertin (0.1 ml/10g body weight; Sigma-Aldrich Corporation, United States) through intraperitoneal injection. To generate I/R injury, the left anterior descending coronary artery (LAD) was ligated with 7-0 nylon for 45 min and then was removed. For the sham group, a suture was passed under the LAD but without ligation. According to the experimental requirements, at different time points of cardiac I/R, the mice were anesthetized for assessing heart function by echocardiographic measurement. All the mice survived during the process of I/R injury after the operation."
item1665 "All the mice (n = 12) were equally and randomly divided into the HCT-scr and HCT-shMETTL3 group. 3 × 106 HCT-scr or HCT-shIGF2BP3 cells suspended in 100 uL PBS were injected subcutaneously from the axilla of each nude mice. After 1 weeks, the long (L) and short (S) diameter of the tumors were measured with vernier caliper every 3 days (tumor volume = L*S2/2). The growth curve of subcutaneous tumors was drawn on the basis of the measured tumor volume. All mice were killed after 17 days since injection of colon cancer cells and subcutaneous tumors were removed completely."
item1666 "For the subcutaneous transplantation model, sh-control, sh-FTO, NC-OE and ERBB2-OE KYSE150 stable cells (6 × 106 per mouse, n = 3 for each group) were diluted to 100 uL PBS + 100 uL Matrigel (BD) and subcutaneously injected to two points in the middle and upper groin of immunodeficient mice to study tumor growth. When the tumor volume reached ~ 1000 mm3 in each group, the nude mice were killed, and the tumors were excised and weighed for histology and further study. The tumor volume was calculated by the formula V = 1/2 × large diameter × (small diameter)2. For a model of lung metastasis in vivo, nude mice were injected with 100 uL WT (wide type), sh-FTO, ERBB2-OE and sh-FTO+ERBB2-OE KYSE150 stable cells (1 × 106 cells per mouse, n = 3 for each group) through tail vein, respectively. Six weeks after injection, the mice were killed and analyzed for metastatic lung tumors."
item1667 .
item1668 .
item1669 .
item1670 "Female C57BL/6J mice (14 weeks old) were treated with bilateral ovariectomy under general anesthesia. Eight weeks after surgery, tibial plateau was harvested and the structure were evaluated with a SCANCO Medical uCT 40 scanner."
item1671 .
item1672 .
item1673 .
item1674 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
item1675 "For the purpose of enhancing the overall randomization of the experiment, a random comparison table had been employed. Accordingly, 5-wk-old male nude athymic BALB/c nu/nu mice (Slack, Shanghai, China) were randomly divided into two parts including a control group (NC) and the experimental group METTL14-OE. For developing subcutaneous xeno transplantation model, 5 × 106 HGC-27 cells stably transfected with NC or METTL14 overexpression were subcutaneously incorporated for 5-week-old BALB/c nude mice. The mice experienced euthanasia after 27 days of inoculation and obtained xenografts's mass was obtained. Tumor volume over three days was obtained. To create mouse STAD liver metastasis orthotopic tumor model, 1 × 106 HGC-27 cells under stable transfection with NC or METTL14 overexpression were added to subserosal gastric wall of BALB/c nude mice."
item1676 "BALB/c-nu mice were grouped as follows to detect tumor growth: HeLa-sh-NC [mice inoculated with scramble shRNA-infected control HeLa cells (n = 5)] and HeLa-sh-CENPK [mice inoculated with CENPK-targeted shRNA-infected HeLa cells (n = 5)]. The mice were subcutaneously inoculated with 5 × 106 cells/100 uL in the flank. The longest and the shortest diameters of the growing tumors were measured every 3 d with a caliper, and the tumor volume (V) was counted by the following equation: V = (the longest diameter × the shortest diameter2)/2. The mice were grouped as follows to evaluate the tumor-initiating frequency and were inoculated with a series of 5 × 105, 2 × 105, and 5 × 104 cells subcutaneously: HeLa-sh-NC (n = 6); and HeLa-sh-CENPK (n = 6). The mice bearing subcutaneous xenograft tumors were grouped as follows to evaluate tumor chemoresistance: HeLa-sh-NC (n = 10); HeLa-sh-CENPK (n = 10); HeLa-sh-NC + cisplatin [mice inoculated with scramble shRNA-infected control HeLa cells and 3 mg/kg of cisplatin once a week intraperitoneally (ip) for 6 weeks (n = 10)]."
item1677 "Stable H1299 cell lines diluted into 100uL were injected subcutaneously into 5-week-old nude mice (n = 5) at the final concentration of 3 × 106 cells. Mice were sacrificed 4 weeks later, and tumors were weighed and photographed."
item1678 "For xenograft models, 5 × 106 BCPAP or KTC-1 cells from each group were injected subcutaneously into the flanks of female BALB/c nude mice (4-6 weeks old, Shanghai SLAC Laboratory Animal, China, n = 5 per group) in a volume of 150 uL PBS. Tumor growth was measured with a digital caliper every 4 days and calculated using the following formula: (length × width2)/2. To study the effect of IL-8 on tumor growth in vivo, scramble or shMETTL3 BCPAP cells were implanted hypodermically into BALB/c nude mice (2 × 106 cells in 150 uL PBS, n = 10 per group). When palpable tumors formed on day 14, mice were treated with DMSO or the IL-8 inhibitor SB225002 (10 mg/kg) by intraperitoneal injection 3 times per week for 3 weeks. Six weeks post-injection, the mice were sacrificed, and the tumors were collected to analyze the frequency of TANs by flow cytometry. For the lung metastasis model, BCPAP and KTC-1 cells (2 × 106 cells in 100 uL PBS) with the corresponding vectors were injected into the tail veins of BALB/c nude mice. Eight weeks after injection, the mice were euthanized, and metastatic lung nodules were analyzed (n = 5 for each group)."
item1679 "For xenograft models, 5 × 106 BCPAP or KTC-1 cells from each group were injected subcutaneously into the flanks of female BALB/c nude mice (4-6 weeks old, Shanghai SLAC Laboratory Animal, China, n = 5 per group) in a volume of 150 uL PBS. Tumor growth was measured with a digital caliper every 4 days and calculated using the following formula: (length × width2)/2. To study the effect of IL-8 on tumor growth in vivo, scramble or shMETTL3 BCPAP cells were implanted hypodermically into BALB/c nude mice (2 × 106 cells in 150 uL PBS, n = 10 per group). When palpable tumors formed on day 14, mice were treated with DMSO or the IL-8 inhibitor SB225002 (10 mg/kg) by intraperitoneal injection 3 times per week for 3 weeks. Six weeks post-injection, the mice were sacrificed, and the tumors were collected to analyze the frequency of TANs by flow cytometry. For the lung metastasis model, BCPAP and KTC-1 cells (2 × 106 cells in 100 uL PBS) with the corresponding vectors were injected into the tail veins of BALB/c nude mice. Eight weeks after injection, the mice were euthanized, and metastatic lung nodules were analyzed (n = 5 for each group)."
item1680 "For xenograft models, 5 × 106 BCPAP or KTC-1 cells from each group were injected subcutaneously into the flanks of female BALB/c nude mice (4-6 weeks old, Shanghai SLAC Laboratory Animal, China, n = 5 per group) in a volume of 150 uL PBS. Tumor growth was measured with a digital caliper every 4 days and calculated using the following formula: (length × width2)/2. To study the effect of IL-8 on tumor growth in vivo, scramble or shMETTL3 BCPAP cells were implanted hypodermically into BALB/c nude mice (2 × 106 cells in 150 uL PBS, n = 10 per group). When palpable tumors formed on day 14, mice were treated with DMSO or the IL-8 inhibitor SB225002 (10 mg/kg) by intraperitoneal injection 3 times per week for 3 weeks. Six weeks post-injection, the mice were sacrificed, and the tumors were collected to analyze the frequency of TANs by flow cytometry. For the lung metastasis model, BCPAP and KTC-1 cells (2 × 106 cells in 100 uL PBS) with the corresponding vectors were injected into the tail veins of BALB/c nude mice. Eight weeks after injection, the mice were euthanized, and metastatic lung nodules were analyzed (n = 5 for each group)."
item1681 "For xenograft models, 5 × 106 BCPAP or KTC-1 cells from each group were injected subcutaneously into the flanks of female BALB/c nude mice (4-6 weeks old, Shanghai SLAC Laboratory Animal, China, n = 5 per group) in a volume of 150 uL PBS. Tumor growth was measured with a digital caliper every 4 days and calculated using the following formula: (length × width2)/2. To study the effect of IL-8 on tumor growth in vivo, scramble or shMETTL3 BCPAP cells were implanted hypodermically into BALB/c nude mice (2 × 106 cells in 150 uL PBS, n = 10 per group). When palpable tumors formed on day 14, mice were treated with DMSO or the IL-8 inhibitor SB225002 (10 mg/kg) by intraperitoneal injection 3 times per week for 3 weeks. Six weeks post-injection, the mice were sacrificed, and the tumors were collected to analyze the frequency of TANs by flow cytometry. For the lung metastasis model, BCPAP and KTC-1 cells (2 × 106 cells in 100 uL PBS) with the corresponding vectors were injected into the tail veins of BALB/c nude mice. Eight weeks after injection, the mice were euthanized, and metastatic lung nodules were analyzed (n = 5 for each group)."
item1682 .
item1683 .
item1684 "PCa cells carrying the transfected plasmid were subcutaneously injected into immunodeficient mice at a rate of 1 × 106 cells per mouse according to a previous study. Tumor formation in the two groups of mice was observed and recorded by a designated personnel every day, and the volume of the tumors was measured. The nude mice were sacrificed 21 days after tumor formation, and the tumors were removed to measure their volume and weight."
item1685 .
item1686 "For the mouse lung metastasis model, SUM-1315 cells (2 × 106/0.2 mL) expressing NC, shKIAA1429, SNAIL, or shKIAA1429+SNAIL were injected into the nude mice through the tail vein."
item1687 "For the mouse lung metastasis model, SUM-1315 cells (2 × 106/0.2 mL) expressing NC, shKIAA1429, SNAIL, or shKIAA1429+SNAIL were injected into the nude mice through the tail vein."
item1688 "The GC cell line MKN-45 stably infected with lentivirus expressing sh-HBXIP was prepared into 5 × 107 cells/mL cell suspension. The cell suspension was injected into the left axilla of nude mice using a 1 mL syringe as the sh-HBXIP group (n = 6). The GC cell line MKN-45 infected with the lentivirus expressing sh-NC was dispersed into the cell suspension, which was injected into nude mice as the sh-NC group (n = 6). Tumor growth was observed and data were recorded after inoculation. On the 26th day, all nude mice were euthanized by cervical dislocation and the tumors were resected and weighed."
item1689 .
item1690 "Female SCID-Beige mice (6 weeks old) were purchased from Vitalriver. AN3CA cells with FTO overexpression or silencing or the appropriate controls (1 × 106) were injected into the lower abdominal cavity of mice (n = 5 mice/group). After 4 weeks, the mice were sacrificed, and tumours were harvested, weighed and photographed."
item1691 "The diabetic model was constructed by a single intraperitoneal injection of streptozotocin (65 mg/kg), which imitates a model of type 1 diabetes. The fasting blood glucose was measured one week after injection. Only rats with glucose levels higher than 16.7 mmol/L were defined as diabetic. Cardiac function was investigated seven days following the last treatment, and the heart tissues were then isolated for expression analyses. The lentivirus vector used for silencing or overexpressing specific genes were dissolved in 50uL saline at the concentration of 1 × 109 TU with one dose after the animal model was established. NLRP3 inhibitor MCC950 (10 mg/kg) was intraperitoneally injected 30 min before streptozotocin treatment."
item1692 "About 5× 106 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4 week-old, 18-20 g). When the average tumor size reached approximately 100mm3 (after 1 week), mice were then randomized into two groups and treated with cisplatin (5 mg/kg) or normal saline (NS) weekly."
item1693 "4-week-old female BALB/c nude mice were used for HuCC-T1 tumor xenograft models and 4-week-old female B-NDG mice (Biocytogen, Beijing, China) were used for HCCC-9810 tumor xenograft models. 1 × 107 HuCC-T1 or HCCC-9810 cells were resuspended in 100 ul PBS with Matrigel (1:1), and injected into the right flank of mice (n = 6/group)."
item1694 "Lentiviruses containing sh-METTL-14 and its negative control (RiboBio Co., Ltd., Guangzhou, China) were transduced into CAL27 cells and stably transduced cells were screened using puromycin. CAL27 cells (3 × 106 cells/mouse) were subcutaneously inoculated into the posterior flank of each mouse (N = 12/group)."
item1695 "BCPAP cells (5×106) were introduced into the mice by means of subcutaneous injection through the flank area. STEAP2-saRNA or NC-saRNA (n = 6 for each group) was given by intratumoral multipoint injection at an interval of 3 days (5 injections in total) using an in vivo transfection reagent (Entranster -in vivo, Engreen, China) as per the vendor-provided protocol. Tumor volume (V) was monitored and calculated as follows: V = (L×W2)/2. For the in vivo tumor metastasis assay, BCPAP cells (5×106 cells) were administrated into mice through the tail vein. STEAP2-saRNA or NC-saRNA (n = 6 for each group) was given via tail vein injection at an interval of 3 days (8 injections in total)."
item1696 .
item1697 .
item1698 .
item1699 .
item1700 .
item1701 "Male BALB/c-nu/nu mice, 9-10 weeks of age, were acclimatized for a week prior to the experiments. Nude mice (6 each group) were subcutaneously inoculated with 4 × 106 SCC-25 or SCC-15 cells through an injection into the center of the back, which consistently caused tumor formation within 1 week of inoculation. To monitor the initial tumor appearance, animals were observed every day. After tumor appeared, measurements were made every week with a caliper. After 28 days, mice were sacrificed and tumors were dissected out to be weighed."
item1702 "Ten four-week-old BALB/c male nude mice (GemPharmatech, Jiangsu, China) were subcutaneously injected with control Huh7 cells 2 × 106 (left-back) and stable knockdown of YTHDF1 Huh7 cells 2 × 106 (right-back). These cells were respectively premixed with 50 ul Matrigel (Corning, 354,234) in 100 ul PBS."
item1703 "Ten four-week-old BALB/c male nude mice (GemPharmatech, Jiangsu, China) were subcutaneously injected with control Huh7 cells 2 × 106 (left-back) and stable knockdown of YTHDF1 Huh7 cells 2 × 106 (right-back). These cells were respectively premixed with 50 ul Matrigel (Corning, 354,234) in 100 ul PBS."
item1704 "For the subcutaneously transplanted tumor model, wild-type, PRMT5-overexpressing or doxorubicin-resistant MDA-MB-231 cells (5 × 106 per mouse, n = 5-7 for each group) were diluted in 100 uL of phosphate-buffered saline (PBS) plus 100 uL of Matrigel (BD Biosciences) and subcutaneously injected into female nude mice to investigate tumor growth. When all tumor volumes reached 100 mm3, the mice were randomly assigned and treated with the indicated drugs. In the experiment, doxorubicin was administered once a week via intravenous tail vein injection at 2 mg/kg body weight, and tadalafil was administered daily via oral gavage at 2 mg/kg body weight. Tumor volume was measured every 3 days using a digital caliper and calculated using the formula V = 1/2 × (diameter) × (smaller diameter)2. The mice were euthanized 27 days after injection."
item1705 .
item1706 .
item1707 "For subcutaneous xenotransplanted tumor models, cells were injected subcutaneously (5 × 106 for MHCC97H or 1×106 for PLC cells per mouse)."
item1708 .
item1709 Tumour xenograft models were established in nude mice bearing: SAS cells cells stably transfected with METTL3-shRNA and the corresponding control vector. The different HNSCC cells (5 × 106) were subcutaneously injected into the right axilla of nude mice (n = 6 per group).
item1710 .
item1711 "Cells were trypsinized and resuspended in DMEM at a consistence of 1 × 107 cells/ml. A total of 1 × 106 cells were injected into flank of mice. 27 days after injection, tumors were removed for paraffin-embedded sections."
item1712 "Cells were trypsinized and resuspended in DMEM at a consistence of 1 × 107 cells/ml. A total of 1 × 106 cells were injected into flank of mice. 27 days after injection, tumors were removed for paraffin-embedded sections."
item1713 .
item1714 .
item1715 .
item1716 .
item1717 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1718 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1719 "To generate an AMI mouse model, mice were anesthetised by intraperitoneal injection of sterile pentobarbital sodium at 50 mg/kg body weight."
item1720 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1721 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1722 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1723 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1724 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
item1725 "Stable short hairpin (shRNA)-expressing MGC-803 cells (3 × 106) were suspended in 0.1 mL PBS and injected into the flanks of BALB/c mice (n = 8 mice/group) at 5-6 weeks of age. For the flanks injected mice, tumor growth was examined every 3 days. After 5 weeks, mice were sacrificed by cervical dislocation, and weight of xenografts was tested.BALB/c mice were randomly divided into three groups (n = 4 mice/group). A total of 3 × 106 stable shRNA-expressing MGC-803 cells were resuspended in 0.1 mL PBS and injected into the abdominal cavity. After 4 weeks, mice were sacrificed by cervical dislocation, abdominal cavities were opened, and the numbers of implantation metastasis were counted.For the pulmonary metastasis model, NOD/SCID mice were randomly divided into three groups (n = 4 mice/group). A total of 1 × 105 stable shRNA-expressing MGC-803 cells were resuspended in 0.1 mL PBS and injected into the lateral tail vein. After 7 weeks, mice were sacrificed by cervical dislocation, and lungs were extracted and fixed 4% paraformaldehyde in PBS. Paraffin embedding, sectioning, and staining with hematoxylin and eosin were performed. Visible lung metastases were measured and counted using a microscope."
item1726 "Specifically, rats were anesthetized with an intraperitoneal injection of 4% pentobarbital sodium (35 mg/kg) after weight measurement. To expose the posterior vertebral arch from T8 to T12, an incision was subsequently made on the skin along the dorsomedial line to the aponeurosis and muscle plane. Laminectomy (3 mm) was performed from the caudal end of the T10 vertebra to the caudal end of the T11 vertebra under a dissection stereomicroscope. Infinite Horizons impactor (Infinite Horizons, L.L.C., Lexington, KY, USA) was utilized to induce contusion SCI at the force of 60 kdyn/cm . The incision was sutured, followed by intramuscular injection of 20000 units of penicillin once a day for three days. Incisions of rats in the sham group (N = 10) were sutured after skin incision without modeling surgery and related treatment. SCI rat model was established and SCI rats were assigned to the following groups (N = 10 per group): SCI group (SCI treatment), SCI + sh-NC group (injected with silencing negative control lentivirus after SCI treatment), SCI + sh-METTL14 + sh-EEF1A2 group (injected with silencing EEF1A2 and silencing METTL14 lentivirus after SCI treatment), SCI + oe-NC group (injected with overexpressed EEF1A2 NC lentivirus after SCI treatment), SCI + oe-EEF1A2 group (injected with overexpressed EEF1A2 lentivirus after SCI treatment), SCI + oe-EEF1A2 + H2O group [injected with overexpressed EEF1A2 lentivirus and treated with 50 mg/kg (i.p.) H2O after SCI treatment] and SCI + oe-EEF1A2 + Perifosine group [injected with overexpressed EEF1A2 lentivirus and treated with 50 mg/kg (i.p.) Perifosine after SCI treatment . Lentivirus treatment was conducted three days following laminectomy (on day 0, 1, and 2). sh-NC, sh-METTL14, sh-EEF1A2, oe-NC, and oe-EEF1A2 lentivirus (50 uL/day, 100 nmoL/mL; RiboBio, Guangzhou, China) were intrathecally injected through lumbar puncture for 15 min per day."
item1727 "Male SD rats weighing 200-250 g were anesthetized using 4% isoflurane in 70% N2O and 30% O2 with a mask. A midline incision was made in the neck, the left external carotid artery (ECA) was carefully exposed and dissected, a monofilament nylon suture with a diameter of about 0.22 mm was inserted from the ECA into the internal carotid artery, and the left middle cerebral artery (MCA) was blocked. After occlusion for 90 minutes, the suture was removed for reperfusion, and ECA was ligated to close the wound. Sham-operated rats underwent the same surgery except for suture insertion. Rats were maintained on top of a warming pad (RWD, 69003) during the above procedures. The breathing machine was used to monitor the respiration of rats. The rats were returned to a heated cage during the recovery phase with free access to food and water."
item1728 .
item1729 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
item1730 .
item1731 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
item1732 .
item1733 "Nude mice were randomly divided into five groups of six mice each, and the mice in each group were received subcutaneous injections of shFTO, scrambled shNC, FTO OE, and vector KYSE510 cells (5×106 tumor cells/mouse), respectively. The tumor size and weight of mice were measured 1 week later, which was recorded as day 0, and then measured once every other day; and the tumor volumes were calculated using the following formula: (length×width2)/2.When the tumor maximum diameter was close to 15 mm, the mice were euthanized and the tumor tissues were collected for immunohistochemistry analysis."
item1734 .
item1735 .
item1736 "NOD/SCID immune-deficient mice were purchased from Shanghai Experimental Animal Center. 2 * 106 MCF-7 cells transduced with sh-NC or sh-YTHDF1-were subcutaneously injected into the mice (5/group). Tumor width and length were measured every 7 days. Tumor volume = (length * width2)/2. After 7 weeks, mice were sacrificed, and the weight of tumors was detected. Xenografts were collected for HE staining, immunohistochemistry staining and western blot analysis.For spontaneous lung metastasis assay, 4 * 106 sh-NC or sh-YTHDF1#2 transduced MCF-7 cells were injected into the mammary fat pads of the NOD/SCID mice (5/group). The primary tumor was removed when its volume reached 150 mm3. The mice were sacrificed, and lung metastasis nodules were counted 12 weeks after the removal."
item1737 "For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in ""Animal Experiments"". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed."
item1738 "For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in ""Animal Experiments"". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed."
item1739 "For the in vivo brain and bone extravasation and seeding assays, cancer cells labeled with CMFDA C2925 (Thermo fisher scientific) or GFP were injected intracardially into the nude mice. Cell number and injection procedure were described in ""Animal Experiments"". For the in vivo lung extravasation and seeding assays, cancer cells labeled with GFP (2.5 × 105 cells/mouse) were injected into the tail vein of nude mice. At 24 or 48 hrs later, the mice were sacrificed."
item1740 "Approximately 2×106 PCa cells (DU145 transfected with shFTO and shNC) were injected subcutaneously in mice. The tumor volume (V = (0.5*length*width2)) was measured with Vernier caliper every week. Ten mice were randomly divided into two groups, 2×106 cells transfected with shFTO and shNC were resuspended with 100 uL PBS and injected into the mouse tail vein to create a metastatic model. After 7 weeks, the mice were anesthetized, and D-luciferin (#D-Luciferin, Apexbio) was injected intraperitoneally, then used the IVIS imaging system (Caliper Life Sciences) to visualize the luciferase signal."
item1741 "To generate doxycycline-inducible skeletal muscle-specific FTO deletion mice, FTOflox/flox mice were crossed with HSA-Cre mice to generate FTOflox/+ HSA-Cre mice, which were then crossed to FTOflox/flox mice to generate FTOflox/flox and FTOflox/flox HSA-Cre mice."
item1742 .
item1743 .
item1744 .
item1745 .
item1746 "Female BALB/c nude mice (ages 4-5 weeks, 18-20 g) were purchased from the Charles River Laboratories. For the tumor growth model, 1 × 106 HONE-1 sh-NC or sh-ZFAS1 cells were injected into the axilla of the mice, and the tumor size was measured every 3 days. On day 30, the mice were killed, and the tumors were dissected and weighed."
item1747 .
item1748 .
item1749 .
item1750 .
item1751 "A total of 30 BALB/c nude mice were chosen and assigned to three groups: (1) control (injected with 0.2 mL PBS), (2) si-NC (injected with si-NC transfected SGC7901 cells) and (3) si-IGF2BP2 (injected with si-IGF2BP2 transfected SGC7901 cells (n = 5 per group). 2 × 106 SGC7901 cells were injected into the left right back of each mouse through subcutaneous injection. Tumor sizes were recorded once per week. After 28 days, the mice were euthanized, and tumor tissues were weighted."
item1752 .
item1753 .
item1754 "Five-week-old female BALB/c nude mice (Charles Rivers, Beijing, China) were selected for the experiments. U87 cells (5 × 105) transfected with an empty vector, YTHDF2 overexpression, or METTL3 overexpression vectors were suspended in PBS and injected into the right frontal node of nude mice. The inoculation position was 2 mm lateral and 2 mm posterior to the anterior fontanel. Tumor size was estimated from luciferase volume measurements and MRI. The mice were sacrificed when they exhibited disturbed activity or convulsion. The brain was then harvested and embedded in paraffin."
item1755 "Five-week-old female BALB/c nude mice (Charles Rivers, Beijing, China) were selected for the experiments. U87 cells (5 × 105) transfected with an empty vector, YTHDF2 overexpression, or METTL3 overexpression vectors were suspended in PBS and injected into the right frontal node of nude mice. The inoculation position was 2 mm lateral and 2 mm posterior to the anterior fontanel. Tumor size was estimated from luciferase volume measurements and MRI. The mice were sacrificed when they exhibited disturbed activity or convulsion. The brain was then harvested and embedded in paraffin."
item1756 "Five-week-old female BALB/c nude mice (Charles Rivers, Beijing, China) were selected for the experiments. U87 cells (5 × 105) transfected with an empty vector, YTHDF2 overexpression, or METTL3 overexpression vectors were suspended in PBS and injected into the right frontal node of nude mice. The inoculation position was 2 mm lateral and 2 mm posterior to the anterior fontanel. Tumor size was estimated from luciferase volume measurements and MRI. The mice were sacrificed when they exhibited disturbed activity or convulsion. The brain was then harvested and embedded in paraffin."
item1757 "Five-week-old female BALB/c nude mice (Charles Rivers, Beijing, China) were selected for the experiments. U87 cells (5 × 105) transfected with an empty vector, YTHDF2 overexpression, or METTL3 overexpression vectors were suspended in PBS and injected into the right frontal node of nude mice. The inoculation position was 2 mm lateral and 2 mm posterior to the anterior fontanel. Tumor size was estimated from luciferase volume measurements and MRI. The mice were sacrificed when they exhibited disturbed activity or convulsion. The brain was then harvested and embedded in paraffin."
item1758 "After randomly assignment and anesthetization, nude mice were injected with 5 × 106 cells suspended in 100 uL PBS into the tail vein (n = 5 per group)."
item1759 "After randomly assignment and anesthetization, nude mice were injected with 5 × 106 cells suspended in 100 uL PBS into the tail vein (n = 5 per group)."
item1760 .
item1761 .
item1762 "The mice were fed in an SPF environment (cycle of 12-h light and 12-h dark) with a free diet. All the mice were adaptively fed for 5 days before experiments and randomly divided into the following four groups: the siNC+MC group (n = 10), the METTL3 siRNA1+MC group (n = 10), siNC+M group (n = 10) and the METTL3 siRNA1+M group (n = 10). Then, the right forelimb of mice in the siNC+MC group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with siNC and MC; the right forelimb of mice in the METTL3 siRNA1+MC group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with METTL3 siRNA1 and MC; the right forelimb of mice in the siNC+M group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with siNC and M; the right forelimb of mice in the METTL3 siRNA1+M group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with METTL3 siRNA1 and M."
item1763 .
item1764 .
item1765 .
item1766 "Mice (male and 6 weeks old) were subcutaneously injected with NSCLC cells (1.0*106 cells/200 uL). The mice were terminated after 4 weeks of induction, and the tumor volume and tumor weight were measured."
item1767 "The mice were divided into control group and ALKBH5-overexpressing group (9 mice per group). ALKBH5-overexpressing and control A549 cells (3 × 106 cells/mouse) in 200 uL PBS were intravenously (i.v.) injected into the lateral tail vein of mice. At every 5th day post-inoculation, TGF-Beta-1 (4 ug/kg body weight) was intraperitoneally (i.p.) injected to promote tumor cell metastasis. Eight weeks later, the mice were euthanized, and then their lungs and livers were taken out and fixed in Bouin's solution (Sigma Aldrich, HT101128) or 4% Paraformaldehyde (Beyotime, p0099, Shanghai, China) for macroscopically metastatic nodule analysis."
item1768 "The mice were divided into control group and ALKBH5-overexpressing group (9 mice per group). ALKBH5-overexpressing and control A549 cells (3 × 106 cells/mouse) in 200 uL PBS were intravenously (i.v.) injected into the lateral tail vein of mice. At every 5th day post-inoculation, TGF-Beta-1 (4 ug/kg body weight) was intraperitoneally (i.p.) injected to promote tumor cell metastasis. Eight weeks later, the mice were euthanized, and then their lungs and livers were taken out and fixed in Bouin's solution (Sigma Aldrich, HT101128) or 4% Paraformaldehyde (Beyotime, p0099, Shanghai, China) for macroscopically metastatic nodule analysis."
item1769 "The mice were divided into control group and ALKBH5-overexpressing group (9 mice per group). ALKBH5-overexpressing and control A549 cells (3 × 106 cells/mouse) in 200 uL PBS were intravenously (i.v.) injected into the lateral tail vein of mice. At every 5th day post-inoculation, TGF-Beta-1 (4 ug/kg body weight) was intraperitoneally (i.p.) injected to promote tumor cell metastasis. Eight weeks later, the mice were euthanized, and then their lungs and livers were taken out and fixed in Bouin's solution (Sigma Aldrich, HT101128) or 4% Paraformaldehyde (Beyotime, p0099, Shanghai, China) for macroscopically metastatic nodule analysis."
item1770 "Four-week-old male BALB/c nude mice were used for animal experiments. UM-UC3 cells (2×106 cells per mouse) stably overexpressing miR-5581-3p and NC were injected into the mice to establish the subcutaneous implantation model. Tumor size was measured by a caliper every week, and tumor volume was calculated by the formula: V = (width2×length×0.52). As for the tumor metastasis model, UM-UC3 cells (1×106 cells per mouse) were injected into each mouse via the tail vein. The subcutaneous implantation model used 8 nude mice, whereas the tumor metastasis model used 10 nude mice. Assessment of tumor size and observation of metastasis tumors were done via intraperitoneal inoculation with 15mg/mL, XenoLight D-luciferin Potassium Salt (100 uL; PerkinElmer) with the IVIS Spectrum animal imaging Platform (PerkinElmer) in every mouse. Eventually, mice were sacrificed for tumors and metastases."
item1771 "Female C57BL/6 mice (6-8 weeks) were intraperitoneally injected with 2 ml 3% sterile sodium thioglycolate solution. After 3 days, the cells in the abdominal cavity were collected, centrifugated and maintained in DMEM with 10% (vol/vol) FBS."
item1772 "Female C57BL/6 mice (6-8 weeks) were intraperitoneally injected with 2 ml 3% sterile sodium thioglycolate solution. After 3 days, the cells in the abdominal cavity were collected, centrifugated and maintained in DMEM with 10% (vol/vol) FBS."
item1773 .
item1774 .
item1775 "A total of 5 × 106 stably transfected HGC-27 cells were subcutaneously injected into the right axillary fossa of nude mice. Tumor volume was measured every 3 days and calculated with the following formula: V = (L × W2)/2 cm2 (V, tumor volume; L, length; W, width). The mice were sacrificed at 3-4 weeks after injection, and the tumors were weighed. For the lung metastasis model, 5 × 106 stably transfected HGC-27 cells were injected into the tail veins of nude mice. Forty-five days later, the mice were sacrificed, and the lungs were dissected to examine the histopathological metastatic loci. The peritoneal dissemination ability of GC cells was evaluated via intraperitoneal injection. A total of 5 × 106 stably transfected HGC-27 cells in 500 uL of PBS were injected into the peritoneal cavity of BALB/c nude mice. Mice were carefully monitored until they were killed at 4 weeks, at which point peritoneal metastases were examined and recorded."
item1776 "Control vector, TUSC7 knockout, FLI-06 treated H1975 cells (1*107) cells were suspended in 100 uL of serum-free DMEM medium (Hyclone, USA), mixed with matrix gel (Corning, USA), and then were injected subcutaneously. The changes in the tumor size were recorded every 3 or 5 days."
item1777 "To study the effect of miR-30d on liver metastasis of PDACs, 5 × 106 cells in 50 uL of PBS were injected into the spleens of nude mice (6 mice per group). Anesthetized mice were injected intraperitoneally with D-luciferin (150 mg/kg) every other week and imaged using an IVIS 100 imaging system (Xenogen, CA, USA) 10 min after the injection. The mice were sacrificed and their liver metastases were checked by standard histological examination 8-9 weeks after injection."
item1778 .
item1779 .
item1780 "HCC827 (3×106) cells suspended in 100 uL of PBS were injected into the left inguen of female Balb/c nude mice (body weight 18-20 g; age 6 weeks; Beijing Huafukang Bioscience Co., Inc.). When the tumor volumes reached 50-100 mm3 on the 10th posttransplantation day, the mice were randomized into four groups (10 mice per group) and were intragastrically administered vehicle (normal saline), crizotinib (25 mg/kg body weight), chidamide (5 mg/kg), or the combination of the two drugs daily for 21 days. The tumor volumes and body weights of the mice were measured every 3 days."
item1781 "HCC827 (3×106) cells suspended in 100 uL of PBS were injected into the left inguen of female Balb/c nude mice (body weight 18-20 g; age 6 weeks; Beijing Huafukang Bioscience Co., Inc.). When the tumor volumes reached 50-100 mm3 on the 10th posttransplantation day, the mice were randomized into four groups (10 mice per group) and were intragastrically administered vehicle (normal saline), crizotinib (25 mg/kg body weight), chidamide (5 mg/kg), or the combination of the two drugs daily for 21 days. The tumor volumes and body weights of the mice were measured every 3 days."
item1782 .
item1783 .
item1784 .
item1785 .
item1786 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1787 .
item1788 "RIP1-Tag2 mice were purchased from NCI Mouse Repository (Bethesda, Rockville, MD, USA) and maintained in a C57BL/6N background."
item1789 .
item1790 .
item1791 OG2 (Oct4-GFP reporter) transgenic mice (CBA/CaJ × C57BL/6J) were original from Jackson Laboratory (Mouse strain datasheet: 004654).
item1792 OG2 (Oct4-GFP reporter) transgenic mice (CBA/CaJ × C57BL/6J) were original from Jackson Laboratory (Mouse strain datasheet: 004654).
item1793 .
item1794 "Luciferase-labeled KYSE150 cells (5 × 106) were inoculated into the footpads of BALB/c nude mice (4-5 weeks old, 18-20 g) to establish the popliteal lymphatic metastasis model."
item1795 "Luciferase-labeled KYSE150 cells (5 × 106) were inoculated into the footpads of BALB/c nude mice (4-5 weeks old, 18-20 g) to establish the popliteal lymphatic metastasis model."
item1796 .
item1797 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1798 .
item1799 .
item1800 .
item1801 .
item1802 "For the subcutaneous implantation model, 1 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
item1803 "IMQ-induced psoriatic model was constructed by applying 10 mg per ear 5% IMQ for 8 consecutive days, and 6 ug macrophage-specific control or hsa_circ_0004287 plasmid was topically applied every 2 days (5 mice per group per experiment)."
item1804 "IMQ-induced psoriatic model was constructed by applying 10 mg per ear 5% IMQ for 8 consecutive days, and 6 ug macrophage-specific control or hsa_circ_0004287 plasmid was topically applied every 2 days (5 mice per group per experiment)."
item1805 .
item1806 "Mice in the control group received an i.p. injections of 0.9% NaCl. Mice in the low, medium, and high Mn groups received i.p. injections of 12.5, 25, and 50 mg/kg MnCl2. The volume of administration was 5 mL/kg body weight. The injection was given daily for 2 weeks."
item1807 "MKN74 cells (5×106/tumor) expressing shNC, shYTHDF1-1, or shYTHDF1-2 were suspended in ice-cold 100 ul PBS:Matrigel gel (1:1, v/v) (Corning, USA), and subcutaneously implanted into the right dorsal flank of 4-week-old NOD."
item1808 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
item1809 .
item1810 .
item1811 .
item1812 .
item1813 "Nude mice were subcutaneously injected with 1 × 107 PADI2 depleted or IGF2BP1 depleted Ishikawa cells on the left flanks, and the corresponding control cells on the right flanks."
item1814 "Four-week-old BALB/c female nude mice were purchased from Beijing HFK Bioscience Co.,LTD. The tumor masses were subcutaneously embedded in nude mice and the nude mice were randomly divided into two groups (n=5) when the tumor masses grew to 4 to 5mm in diameter. These two groups were control group and siALKBH5 group."
item1815 "For the subcutaneous implantation model, 5 × 105 stable SLC7A11-knockdown HCCLM3 cells or SLC7A11-vector cells were injected subcutaneously into BALB/C nude mice."
item1816 .
item1817 .
item1818 .
item1819 .
item1820 .
item1821 .
item1822 .
item1823 .
item1824 "To develop the syngeneic model, 1 × 106 MC38-Ctrl or MC38-Gpx4KD cells were injected subcutaneously into the right flank of C57BL/6 mice."
item1825 "To develop the syngeneic model, 1 × 106 MC38-Ctrl or MC38-Gpx4KD cells were injected subcutaneously into the right flank of C57BL/6 mice."
item1826 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old)."
item1827 "For in vivo studies, the shMETTL3 sequence was subcloned into adeno-associated virus (AAV) construct 9 (AAV9). Recombinant AAV9 was manufactured by GenePharma (Shanghai, China). The ectopic endometrium was obtained from a 28-year-old woman who had undergone an operation for ovarian endometriosis. The tissue sample was washed twice with PBS and cut into three 3- to 5-mm pieces, and suspended by PBS.he animals were administered intraperitoneal injections of 30 mg/kg 17-oestradiol every three days after the endometrial injections. Xenografts were generated under bilateral axillae after 14 days with tumour volumes of approximately 10 mm3."
item1828 "For in vivo studies, the shMETTL3 sequence was subcloned into adeno-associated virus (AAV) construct 9 (AAV9). Recombinant AAV9 was manufactured by GenePharma (Shanghai, China). The ectopic endometrium was obtained from a 28-year-old woman who had undergone an operation for ovarian endometriosis. The tissue sample was washed twice with PBS and cut into three 3- to 5-mm pieces, and suspended by PBS.he animals were administered intraperitoneal injections of 30 mg/kg 17-oestradiol every three days after the endometrial injections. Xenografts were generated under bilateral axillae after 14 days with tumour volumes of approximately 10 mm3."
item1829 "Female BALB/c nude mice (4-5 weeks, 18-20 g) were purchased from GemPharmatech (Nanjing, China) and raised in a specific pathogen-free (SPF) experimental animal room. Treated cells (1×106/100 μl PBS) were injected through the tail vein of the nude mice. Pulmonary metastasis was evaluated by bioluminescence imaging at 4 or 8 weeks. Then, the mice were sacrificed, and the lung tissues were imaged and fixed in 4% paraformaldehyde for further analyses."
item1830 Each mouse was intraperitoneally administered according to body weight (20 μg/g of ISO-1 for the CLP+ISO-1 group and the same quantity of 1% DMSO for the CLP+DMSO group) 4-6 h prior to the CLP surgery. Injection of renal parenchyma (5×107 TU/mouse) facilitated lentivirus-mediated gene transfer in the kidneys. The CLP operation was conducted one week after the injection. In vivo tests were conducted in accordance with Chinese regulations on the use and care of laboratory animals.
item1831 "(1) ZBTB7C functional assay in vivo, 2 × 106 MNNG/HOS cells were stably transfected with lentivirus (vector control and si-ZBTB7C, n = 5), and then subcutaneously incubated in BALB/c nude mice; (2) STM2457 intervention. MNNG/HOS cells were implanted subcutaneously first. When tumors reached approximately 80-100 mm3, PBS or STM2457 (30 mg/kg (STM-30), 60 mg/kg (STM-60), n = 4) were administered every 2 days; (3) ZBTB7C effects of STM2457 assay. Mice were assigned to 4 groups (n = 5 per group). Two groups had MNNG/HOS cells stably transfected with lentiviral vectors (Ctrl) and 2 with lentivirus-mediated ZBTB7C overexpression (ZBTB7C-OE), and then mice were treated with control vehicle or STM2457 (60 mg/kg), respectively. The experiments went on for 22 days, and the xenografted tumors were measured, weighted, and characterized by H&E and IHC staining."
item1832 "NCG mice (female, 4-5 weeks old, purchased from Jiangsu GemPharmatech) were acclimated for 1 week and were selected randomly to subcutaneously (s.c.) injected with 100 μl suspensions of 1 × 106 thyroid cancer cell lines. All mice were housed in animal facility under specific pathogen-free conditions. After 7 day, 5 × 106 huPBMCs were intravenously (i.v.) inoculated into mice. Mice were treated intravenously with anti-PD-1 mAb (nivolumab, 200 μg per injection), Combination (nivolumab + anti-CD70 mAb cusatuzumab, 200 μg per injection each), or PBS once a week. The treatment with M2-TAMs EVs or PBS continued in mice at 2ug EVs per injection at the tumor site twice a week [24]. The body weight of mice and the length (mm) and width (mm) of tumors were monitored every 3 or 4 days. Tumor volume (mm3) was calculated as Length × Width2 × 1/2. Tumor growth analyses were limited to 3 to 4 weeks because following this period mice started to show signs of xenograft-versus-host disease (xGVHD). Therefore, after 14 days or 21 days of PBMCs implantation, the mice were euthanized."
item1833 "NCG mice (female, 4-5 weeks old, purchased from Jiangsu GemPharmatech) were acclimated for 1 week and were selected randomly to subcutaneously (s.c.) injected with 100 μl suspensions of 1 × 106 thyroid cancer cell lines. All mice were housed in animal facility under specific pathogen-free conditions. After 7 day, 5 × 106 huPBMCs were intravenously (i.v.) inoculated into mice. Mice were treated intravenously with anti-PD-1 mAb (nivolumab, 200 μg per injection), Combination (nivolumab + anti-CD70 mAb cusatuzumab, 200 μg per injection each), or PBS once a week. The treatment with M2-TAMs EVs or PBS continued in mice at 2ug EVs per injection at the tumor site twice a week [24]. The body weight of mice and the length (mm) and width (mm) of tumors were monitored every 3 or 4 days. Tumor volume (mm3) was calculated as Length × Width2 × 1/2. Tumor growth analyses were limited to 3 to 4 weeks because following this period mice started to show signs of xenograft-versus-host disease (xGVHD). Therefore, after 14 days or 21 days of PBMCs implantation, the mice were euthanized."
item1834 "NCG mice (female, 4-5 weeks old, purchased from Jiangsu GemPharmatech) were acclimated for 1 week and were selected randomly to subcutaneously (s.c.) injected with 100 μl suspensions of 1 × 106 thyroid cancer cell lines. All mice were housed in animal facility under specific pathogen-free conditions. After 7 day, 5 × 106 huPBMCs were intravenously (i.v.) inoculated into mice. Mice were treated intravenously with anti-PD-1 mAb (nivolumab, 200 μg per injection), Combination (nivolumab + anti-CD70 mAb cusatuzumab, 200 μg per injection each), or PBS once a week. The treatment with M2-TAMs EVs or PBS continued in mice at 2ug EVs per injection at the tumor site twice a week [24]. The body weight of mice and the length (mm) and width (mm) of tumors were monitored every 3 or 4 days. Tumor volume (mm3) was calculated as Length × Width2 × 1/2. Tumor growth analyses were limited to 3 to 4 weeks because following this period mice started to show signs of xenograft-versus-host disease (xGVHD). Therefore, after 14 days or 21 days of PBMCs implantation, the mice were euthanized."
item1835 "NCG mice (female, 4-5 weeks old, purchased from Jiangsu GemPharmatech) were acclimated for 1 week and were selected randomly to subcutaneously (s.c.) injected with 100 μl suspensions of 1 × 106 thyroid cancer cell lines. All mice were housed in animal facility under specific pathogen-free conditions. After 7 day, 5 × 106 huPBMCs were intravenously (i.v.) inoculated into mice. Mice were treated intravenously with anti-PD-1 mAb (nivolumab, 200 μg per injection), Combination (nivolumab + anti-CD70 mAb cusatuzumab, 200 μg per injection each), or PBS once a week. The treatment with M2-TAMs EVs or PBS continued in mice at 2ug EVs per injection at the tumor site twice a week [24]. The body weight of mice and the length (mm) and width (mm) of tumors were monitored every 3 or 4 days. Tumor volume (mm3) was calculated as Length × Width2 × 1/2. Tumor growth analyses were limited to 3 to 4 weeks because following this period mice started to show signs of xenograft-versus-host disease (xGVHD). Therefore, after 14 days or 21 days of PBMCs implantation, the mice were euthanized."
item1836 "BACE1-AS knockout SW620 or parental SW620 cells were injected into the inferior Hemi-spleen of the mice. The weights of mice were recorded every 3 days. After 7 weeks, the mice were euthanized. The whole livers of mice were resected and photographed to assess metastatic burden. ."
item1837 "BALB/c-nu mice (5-6 weeks) were purchased from Sun Yat-sen University (SYSU) Animal Center. The mice were housed in a pathogen-free environment at the SYSU Animal Center. To establish a human ESCC xenograft model, 20 BALB/c-nu mice were randomly assigned to four groups, with each group consisting of five mice. Two groups of mice received a subcutaneous injection of either 2.0 × 106 Eca109 cells or 2.0 × 106 sh-METTL3-Eca109 cells. Tumor development was monitored daily. After one week, each mice was subjected to intratumoral injection of 10 μl PBS or Fn (1.0 × 107 CFU) once every two days, three times a week. The mice were sacrifices at the end of two weeks after the injection, and subsequently, the tumor, liver, and lungs were surgically removed. The following formula was used to determine tumor volume: 0.5× (length × width2)."
item1838 .
item1839 .
item1840 .
item1841 "For exercise-trained rats, SD rats were treadmill trained 5 days per week continuing six weeks. In brief, exercise was carried out on a motor-driven treadmill, set at a 10.5% incline, 5 days per week for 6 weeks in an adjoining room maintained at 20 °C. Running duration and speed were gradually increased more than 22 days to 60 min at 30 m/min, corresponding to about 80% VO2 max, and maintained for 2-3 weeks."
item1842 "For exercise-trained rats, SD rats were treadmill trained 5 days per week continuing six weeks. In brief, exercise was carried out on a motor-driven treadmill, set at a 10.5% incline, 5 days per week for 6 weeks in an adjoining room maintained at 20 °C. Running duration and speed were gradually increased more than 22 days to 60 min at 30 m/min, corresponding to about 80% VO2 max, and maintained for 2-3 weeks."
item1843 .
item1844 .
item1845 .
item1846 .
item1847 .
item1848 .
item1849 .
item1850 "Corn oil (control group), 1 mg/kg/day DEHP (low-dose: D1 group), 250 mg/kg/day DEHP (middle-dose: D250 group), and 500 mg/kg/day DEHP (high-dose: D500 group)."
item1851 "Corn oil (control group), 1 mg/kg/day DEHP (low-dose: D1 group), 250 mg/kg/day DEHP (middle-dose: D250 group), and 500 mg/kg/day DEHP (high-dose: D500 group)."
item1852 .
item1853 .
item1854 .
item1855 "For the xenograft implantation model, 2 × 106 MHCC-97H cells were subcutaneously injected into the flank of nude mice."
item1856 "For the xenograft implantation model, 2 × 106 MHCC-97H cells were subcutaneously injected into the flank of nude mice."
item1857 "Five female mice were subjected to a normal pregnancy assay. Uterine tissues of 14 female donor mice were cut up and injected into the abdominal cavity of 28 female recipient mice. After 21 d, 10 male C57 mice were mated with the recipient mice, and the next day, when vaginal plugs were observed, was regarded as day 1. Eight recipient mice were euthanized by cervical dislocation after deep pentobarbital anesthesia on day 8, and the number of blastocysts in the uterus was counted. At the night of day 3, 10 μL of STM2457, a METTL3 inhibitor (Sellcek, S9870, 10 μM) and 10 μL dimethyl sulfoxide (DMSO) were individually injected into the uterine horns of 20 recipient mice. The mice were euthanized on day 8, and the number of blastocysts in the uterus was counted. The uterine tissues were collected and fixed in 4% (w/v) paraformaldehyde for histological and IHC analyses."
item1858 "Five female mice were subjected to a normal pregnancy assay. Uterine tissues of 14 female donor mice were cut up and injected into the abdominal cavity of 28 female recipient mice. After 21 d, 10 male C57 mice were mated with the recipient mice, and the next day, when vaginal plugs were observed, was regarded as day 1. Eight recipient mice were euthanized by cervical dislocation after deep pentobarbital anesthesia on day 8, and the number of blastocysts in the uterus was counted. At the night of day 3, 10 μL of STM2457, a METTL3 inhibitor (Sellcek, S9870, 10 μM) and 10 μL dimethyl sulfoxide (DMSO) were individually injected into the uterine horns of 20 recipient mice. The mice were euthanized on day 8, and the number of blastocysts in the uterus was counted. The uterine tissues were collected and fixed in 4% (w/v) paraformaldehyde for histological and IHC analyses."
item1859 "Mouse pups, along with their nursing mothers, were exposed to 75 ± 2% O2 in an incubator between postnatal day (P) 7 and P12 and were then returned to room air."
item1860 "Mouse pups, along with their nursing mothers, were exposed to 75 ± 2% O2 in an incubator between postnatal day (P) 7 and P12 and were then returned to room air."
item1861 "To explore the changes of MMP3 expression during MCAO/R injury, mice were randomized into three groups, including Sham group (n = 6), MCAO/R-12 h group (n = 6), and MCAO/R-24 h group (n = 6). MCAO surgery was carried following our previous description."
item1862 .
item1863 "The mice were fed under standard SPF conditions. The PANC-1 cells (5 × 106 per mouse) were injected subcutaneously into the right flank regions of the mice. After 21 days of growth, the mice were euthanized and tumor tissue samples were collected. The tumors were fixed in formalin, embedded in paraffin and stained with eosin and hematoxylin (i.e., HE staining."
item1864 "A 26 G pediatric venous indwelling catheter (0.6 mm outer diameter) was used for urethral catheterization. To create intravesical obstruction, a 6-0 nonabsorbable polypropylene suture was gently ligatured. Then, after the suture knot was made, the catheter was carefully removed. Rats in the sham-operated group underwent all of the same procedures, except for the urethral ligation step. At the end of the experiments, the rats were killed humanely."
item1865 .
item1866 .
item1867 .
item1868 .
item1869 .
item1870 .
item1871 .
item1872 .
item1873 .
item1874 .
item1875 .
item1876 "In the orthotopic xenografts, a total of 5 × 105 firefly luciferase-expressing U87 cells were implanted into the frontal lobes of 4-week-old male BALB/c nude mice."
item1877 .
item1878 .
item1879 .
item1880 .
item1881 .
item1882 "6 × 106 logarithmically growing GIST cells resuspended in 100 μL PBS were injected subcutaneously into the flank of 6-week-old female nude mice for tumor growth assays. The following treatments were initiated when the tumor volume reached approximately 300 mm3. (1) IM-sensitive GIST cell lines and IM-resistant GIST cell lines were used to establish a subcutaneous xenograft model of GIST (n = 6 mice/group). When the tumors grew to the required size, mice were treated with IM via drinking water; (2) GIST cells were pretreated with USP13 lentivirus or USP13 control lentivirus. In addition, IM-resistant GIST cells were pretreated with USP13 inhibitors or control. These lentivirus-transfected cells were implanted into subcutaneous mice tumors to construct the USP13 positive, USP13-negative, and control groups (n = 6 mice/group). When the transplanted tumor grew to the required volume, mice in each group were treated with IM via drinking water. (3) IM-resistant GIST cells-xenografted mice and control mice were treated with USP13 inhibitors or with USP13 inhibitors and autophagy inhibitors in combination therapy to assess reversal of IM resistance (n = 6 mice/group). Mice were treated with IM (45 mg/kg/day) and with or without 3-MA (15 mg/kg/day) via drinking water when the tumors grew to the required size. The USP13 inhibitor Spautin-1 was used at 20 mg/kg/day when required."
item1883 "6 × 106 logarithmically growing GIST cells resuspended in 100 μL PBS were injected subcutaneously into the flank of 6-week-old female nude mice for tumor growth assays. The following treatments were initiated when the tumor volume reached approximately 300 mm3. (1) IM-sensitive GIST cell lines and IM-resistant GIST cell lines were used to establish a subcutaneous xenograft model of GIST (n = 6 mice/group). When the tumors grew to the required size, mice were treated with IM via drinking water; (2) GIST cells were pretreated with USP13 lentivirus or USP13 control lentivirus. In addition, IM-resistant GIST cells were pretreated with USP13 inhibitors or control. These lentivirus-transfected cells were implanted into subcutaneous mice tumors to construct the USP13 positive, USP13-negative, and control groups (n = 6 mice/group). When the transplanted tumor grew to the required volume, mice in each group were treated with IM via drinking water. (3) IM-resistant GIST cells-xenografted mice and control mice were treated with USP13 inhibitors or with USP13 inhibitors and autophagy inhibitors in combination therapy to assess reversal of IM resistance (n = 6 mice/group). Mice were treated with IM (45 mg/kg/day) and with or without 3-MA (15 mg/kg/day) via drinking water when the tumors grew to the required size. The USP13 inhibitor Spautin-1 was used at 20 mg/kg/day when required."
item1884 "6 × 106 logarithmically growing GIST cells resuspended in 100 μL PBS were injected subcutaneously into the flank of 6-week-old female nude mice for tumor growth assays. The following treatments were initiated when the tumor volume reached approximately 300 mm3. (1) IM-sensitive GIST cell lines and IM-resistant GIST cell lines were used to establish a subcutaneous xenograft model of GIST (n = 6 mice/group). When the tumors grew to the required size, mice were treated with IM via drinking water; (2) GIST cells were pretreated with USP13 lentivirus or USP13 control lentivirus. In addition, IM-resistant GIST cells were pretreated with USP13 inhibitors or control. These lentivirus-transfected cells were implanted into subcutaneous mice tumors to construct the USP13 positive, USP13-negative, and control groups (n = 6 mice/group). When the transplanted tumor grew to the required volume, mice in each group were treated with IM via drinking water. (3) IM-resistant GIST cells-xenografted mice and control mice were treated with USP13 inhibitors or with USP13 inhibitors and autophagy inhibitors in combination therapy to assess reversal of IM resistance (n = 6 mice/group). Mice were treated with IM (45 mg/kg/day) and with or without 3-MA (15 mg/kg/day) via drinking water when the tumors grew to the required size. The USP13 inhibitor Spautin-1 was used at 20 mg/kg/day when required."
item1885 "6 × 106 logarithmically growing GIST cells resuspended in 100 μL PBS were injected subcutaneously into the flank of 6-week-old female nude mice for tumor growth assays. The following treatments were initiated when the tumor volume reached approximately 300 mm3. (1) IM-sensitive GIST cell lines and IM-resistant GIST cell lines were used to establish a subcutaneous xenograft model of GIST (n = 6 mice/group). When the tumors grew to the required size, mice were treated with IM via drinking water; (2) GIST cells were pretreated with USP13 lentivirus or USP13 control lentivirus. In addition, IM-resistant GIST cells were pretreated with USP13 inhibitors or control. These lentivirus-transfected cells were implanted into subcutaneous mice tumors to construct the USP13 positive, USP13-negative, and control groups (n = 6 mice/group). When the transplanted tumor grew to the required volume, mice in each group were treated with IM via drinking water. (3) IM-resistant GIST cells-xenografted mice and control mice were treated with USP13 inhibitors or with USP13 inhibitors and autophagy inhibitors in combination therapy to assess reversal of IM resistance (n = 6 mice/group). Mice were treated with IM (45 mg/kg/day) and with or without 3-MA (15 mg/kg/day) via drinking water when the tumors grew to the required size. The USP13 inhibitor Spautin-1 was used at 20 mg/kg/day when required."
item1886 "Rats were tested in the Morris Water Maze (MWM) after PND 60. The experimental device was a circular tank with a diameter of 120 cm and depth of 40 cm, containing water held at a constant 23 ± 1 °C. Rats were allowed to swim to a settled platform (a round, clear acrylic panel) for no more than 90 s with its top surface submerged 1.5 cm below the water level. Caramel was dissolved in water to ensuring that rats cannot directly observe the platform position. Rats were released from different positions in the water maze, and each rat performed four trials daily for five days. The escape latency was automatically recorded. On the sixth day, the rats were given a 90-s retention trial in which the platform was removed, and the mean speed, relative time in zone 2 and relative distance in zone 2 were recorded."
item1887 "Rats were tested in the Morris Water Maze (MWM) after PND 60. The experimental device was a circular tank with a diameter of 120 cm and depth of 40 cm, containing water held at a constant 23 ± 1 °C. Rats were allowed to swim to a settled platform (a round, clear acrylic panel) for no more than 90 s with its top surface submerged 1.5 cm below the water level. Caramel was dissolved in water to ensuring that rats cannot directly observe the platform position. Rats were released from different positions in the water maze, and each rat performed four trials daily for five days. The escape latency was automatically recorded. On the sixth day, the rats were given a 90-s retention trial in which the platform was removed, and the mean speed, relative time in zone 2 and relative distance in zone 2 were recorded."
item1888 "3 × 106 CRC cells were inoculated subcutaneously into the right hind leg of BALB/c nude mice. There were 12 nude mice per group. The tumor volume was observed and calculated every 5 days according to the formula: tumor volume = (length × width2)/2. After 30 days, nude mice were euthanatized through intraperitoneal injection of excessive pentobarbital (> 100 mg/kg)."
item1889 "Lentiviral vectors for sh-WTAP#1#2#NC were constructed and transfected into U2OS cells (1 × 106 cells/100 μL) using Lipofectamine 3000 (Invitrogen), followed by subcutaneous injection into the right flank of mice. Tumor volume was measured at the time point of 1st, 2nd, 3rd, and 4th week. At the end of 4th week, the mice were anesthetized by pentobarbital sodium (50 mg/kg) with intraperitoneal injection and sacrificed using euthanasia method."
item1890 .
item1891 0.1 ml of cell suspension containing 2 × 106 luciferase-labeled cells was injected into tail veins.
item1892 0.1 ml of cell suspension containing 2 × 106 luciferase-labeled cells was injected into tail veins.
item1893 .
item1894 "Rats were anesthetized with intraperitoneal injection of 1% sodium pentobarbital (20 mg/kg). For constructing a rat spinal cord hemisection model, the spinal colon was marked on the T9 spinous process and the skin was incised until exposing the T9-10 spinous process. The spinal cord was completely exposed by biting the vertebral plate with biting forceps. The spinal cord was cut on one side with ophthalmic scissors centered on the central canal of the spinal cord."
item1895 "For in vivo lung metastases model, a total of 2 × 106 CRC cells with REG1alpha-overexpression or REG1alpha-knockdown were injected into nude mice via lateral tail vein. Six weeks after injection, the mice were sacrificed and the lung tissues were collected."
item1896 .
item1897 "Female BALB/c nude mice aged 5 weeks were used to perform in vivo proliferation and metastasis assays. For subcutaneous xenograft tumor model, 1×106 6-10B cells in 200 μL PBS were injected into the dorsal flank of each mouse (n = 7 in each group). The long and short diameters of subcutaneous tumors were measured every 3 days until the endpoint. Mice were sacrificed about 4 weeks after injection, and subcutaneous tumors were harvested, weighted and then fixed in 4% paraformaldehyde for subsequent analysis. For inguinal lymph node metastasis model, 1×105 5-8F cells in 30 μL of PBS were injected into the right foot pad of each mouse (n = 7 in each group). Mice were sacrificed about one month after injection. Primary foot pad tumor and metastatic inguinal lymph nodes were harvested and fixed in 4% paraformaldehyde for subsequent assays."
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item1903 "Animal experiments were approved by the Hainan Affiliated Hospital of Hainan Medical University. Male BALB/c mice (Vital River, Beijing, China) were subcutaneously injected with H322 cells (1 × 106) transfected with ov-NC, ov-FTO, ov-GAS5 or ov-FTO + ov-GAS5 (n = 5/group). Tumor volume was measured every 7 days, and mice were sacrificed and the tumor tissues were removed for weighting and analyzing after 35 days. Also, tumor tissues were prepared into paraffin sections to carry out Ki67 immunohistochemistry (IHC) staining using SP Kit (Solarbio, Beijing, China) and TUNEL staining using Colorimetric TUNEL Apoptosis Assay Kit (Beyotime) basing on kit instructions."
item1904 .
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item1906 .
item1907 "Before hyperoxia treatment, mice in the BPD/PVT1 KO group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 at a titer of 1 × 109 pfu/100 μL. Mice in the BPD/PVT1 KO + IL-33 group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 and 5 μL adenovirus vector expressing IL-33."
item1908 "Before hyperoxia treatment, mice in the BPD/PVT1 KO group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 at a titer of 1 × 109 pfu/100 μL. Mice in the BPD/PVT1 KO + IL-33 group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 and 5 μL adenovirus vector expressing IL-33."
item1909 .
item1910 "A total of 5637 cells with ectopic expression of dm6ACRISPR systems were injected subcutaneously (1 × 107 cells/inoculum) into the flanks of 5-wk-old nude mice (Shanghai Model Organisms Center). Tumor formation/growth was assessed until the experimental endpoint, and tumor volume was calculated by the formula: (width)2 × length/2. For tail vein injection, 5637 cells with ectopic expression of dm6ACRISPR systems (5 × 106 cells/0.1 mL PBS) were injected into the lateral tail vein of 5-wk-old nude mice."
item1911 "A total of 5637 cells with ectopic expression of dm6ACRISPR systems were injected subcutaneously (1 × 107 cells/inoculum) into the flanks of 5-wk-old nude mice (Shanghai Model Organisms Center). Tumor formation/growth was assessed until the experimental endpoint, and tumor volume was calculated by the formula: (width)2 × length/2. For tail vein injection, 5637 cells with ectopic expression of dm6ACRISPR systems (5 × 106 cells/0.1 mL PBS) were injected into the lateral tail vein of 5-wk-old nude mice."
item1912 "To evaluate the effect of gemcitabine on the tumor formation ability and gemcitabine/WTAP/MYC axis in vivo, we injected 2×106 PANC1 cells at logarithmic growth phase into the axilla of 4-week-old nude mice for subcutaneous tumor formation. The mice were randomly divided into phosphate buffered saline (PBS) group and gemcitabine treatment group. PBS or gemcitabine was injected into the mice via the abdominal cavity at a concentration of 50 mg/kg once every 5 days. The tumor dimension was measured every 5 days. After 30 days, the tumors were excised and weighed to compare the tumor size and volume, WTAP and MYC mRNA and protein expression was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunofluorescence."
item1913 "To evaluate the effect of gemcitabine on the tumor formation ability and gemcitabine/WTAP/MYC axis in vivo, we injected 2×106 PANC1 cells at logarithmic growth phase into the axilla of 4-week-old nude mice for subcutaneous tumor formation. The mice were randomly divided into phosphate buffered saline (PBS) group and gemcitabine treatment group. PBS or gemcitabine was injected into the mice via the abdominal cavity at a concentration of 50 mg/kg once every 5 days. The tumor dimension was measured every 5 days. After 30 days, the tumors were excised and weighed to compare the tumor size and volume, WTAP and MYC mRNA and protein expression was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunofluorescence."
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item1918 .
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item1924 "Using a 100 μl Hamilton Microliter syringe, all the groups of 1 × 107 luciferase-labeled Huh-7 cells in 50 μl 1 x phosphate-buffered saline (PBS) was injected intracardially in the left ventricle of nu/nu, female 4-6-week-old nude mice."
item1925 "Using a 100 μl Hamilton Microliter syringe, all the groups of 1 × 107 luciferase-labeled Huh-7 cells in 50 μl 1 x phosphate-buffered saline (PBS) was injected intracardially in the left ventricle of nu/nu, female 4-6-week-old nude mice."
item1926 "Animals had free access to standard rodent chow and water. For the subcutaneous xenograft tumor model, transfected cells (5×106) were injected on the sides of the flanks of mice (n=5 per group). Subsequently, 30 days later, the mice were euthanized by cervical dislocation following anesthesia induced by intraperitoneal injection of 0.3% sodium pentobarbital (30 mg/kg). Euthanasia was confirmed by verifying respiratory and cardiac arrest, along with pupil dilation, for a minimum of 10 min. Tumor size and tumor weight were measured (max tumor diameter, 9.6 mm; max area, 82.56 mm2; max volume, 355.01 mm3). For the pulmonary metastasis model, transfected cells (2×106) were injected into the mouse tail veins (n=5 per group). Subsequently, 60 days later, the mice were sacrificed, and lungs were obtained. Finally, lung and tumor tissues were available for H&E staining at room temperature for 5 min or immunohistochemistry staining."
item1927 "Animals had free access to standard rodent chow and water. For the subcutaneous xenograft tumor model, transfected cells (5×106) were injected on the sides of the flanks of mice (n=5 per group). Subsequently, 30 days later, the mice were euthanized by cervical dislocation following anesthesia induced by intraperitoneal injection of 0.3% sodium pentobarbital (30 mg/kg). Euthanasia was confirmed by verifying respiratory and cardiac arrest, along with pupil dilation, for a minimum of 10 min. Tumor size and tumor weight were measured (max tumor diameter, 9.6 mm; max area, 82.56 mm2; max volume, 355.01 mm3). For the pulmonary metastasis model, transfected cells (2×106) were injected into the mouse tail veins (n=5 per group). Subsequently, 60 days later, the mice were sacrificed, and lungs were obtained. Finally, lung and tumor tissues were available for H&E staining at room temperature for 5 min or immunohistochemistry staining."
item1928 "Animals had free access to standard rodent chow and water. For the subcutaneous xenograft tumor model, transfected cells (5×106) were injected on the sides of the flanks of mice (n=5 per group). Subsequently, 30 days later, the mice were euthanized by cervical dislocation following anesthesia induced by intraperitoneal injection of 0.3% sodium pentobarbital (30 mg/kg). Euthanasia was confirmed by verifying respiratory and cardiac arrest, along with pupil dilation, for a minimum of 10 min. Tumor size and tumor weight were measured (max tumor diameter, 9.6 mm; max area, 82.56 mm2; max volume, 355.01 mm3). For the pulmonary metastasis model, transfected cells (2×106) were injected into the mouse tail veins (n=5 per group). Subsequently, 60 days later, the mice were sacrificed, and lungs were obtained. Finally, lung and tumor tissues were available for H&E staining at room temperature for 5 min or immunohistochemistry staining."
item1929 "A total of twenty-seven adult specific-pathogen-free C57BL/6 mice (male, 10 weeks old, 22-25 g) were obtained from the Animal Center of Xi'an JiaoTong University (Xi'an, China). Mice were housed in a standard environment of 22 ± 0.5°C, 70% humidity with a 12-h light/dark cycle and free access to food and water."
item1930 "Eight-week-old male SD rats were purchased from SPF (Beijing) biotechnology co., LTD (Animal certificate number: 1103212101024214414). After the animals were kept for 7 days for environmental adaptation, the production of animal models was started. The rats were divided into 4 groups, control group, sepsis group, siMETTL3 group and sepsis+siMETTL3 group respectively. siMETTL3 was given at a dose of 5 nmol/ time/animal by tail vein injection. Doses were given every 3 days for a total of 18 days (i.e., 6 doses). The sepsis model was established by intraperitoneal injection of LPS (5 mg/kg) 24 h after the end of administration, the survival rate of LPS-treated mice was 75 % [[47], [48], [49]]. The control group was intraperitoneally injected with the same volume of normal saline. Echocardiography was performed 6 h after LPS injection. The animals were anesthetized by isoflavane induction and cardiac functions were evaluated by transthoracic echocardiography with an ultrasound machine (VisualSonics USA vevo 2100). The serum was stored in the refrigerator at 4 °C for 1 h and centrifuged for 3000r for 15 min. The packaged erum was stored at -80 °C for subsequent detection. The apical tissue was cut into 1 mm × 1 mm tissue blocks and placed in the electron microscope liquid at 4 °C for electron microscope detection."
item1931 .
item1932 .
item1933 .
item1934 "Twenty-four mice were randomly divided into three groups (8 mice/group). Random number remainder grouping method was used for random grouping. The mice were whole-body exposed to filtered air (FA), unfiltered air (UA), and concentrated PM2.5 (CA) after 1 week of acclimatization. The FA group served as control. The FA and UA mice were housed in the chambers with or without high efficiency particulate air filters, respectively. CA mice were housed in the chamber with the concentrated PM2.5 using the PM2.5 concentration enrichment system (Beijing Huironghe Technology Co., Ltd, Beijing, China) according to our previously reported procedures (Su et al., 2020). After the last day of exposure, all mice survived and were anesthetized with 1 % pentobarbital sodium, then sacrificed, and lung tissues were separated and weighed. Six random samples every group was applied for in vivo study."
item1935 "Twenty-four mice were randomly divided into three groups (8 mice/group). Random number remainder grouping method was used for random grouping. The mice were whole-body exposed to filtered air (FA), unfiltered air (UA), and concentrated PM2.5 (CA) after 1 week of acclimatization. The FA group served as control. The FA and UA mice were housed in the chambers with or without high efficiency particulate air filters, respectively. CA mice were housed in the chamber with the concentrated PM2.5 using the PM2.5 concentration enrichment system (Beijing Huironghe Technology Co., Ltd, Beijing, China) according to our previously reported procedures (Su et al., 2020). After the last day of exposure, all mice survived and were anesthetized with 2 % pentobarbital sodium, then sacrificed, and lung tissues were separated and weighed. Six random samples every group was applied for in vivo study."
item1936 "Twenty-four mice were randomly divided into three groups (8 mice/group). Random number remainder grouping method was used for random grouping. The mice were whole-body exposed to filtered air (FA), unfiltered air (UA), and concentrated PM2.5 (CA) after 1 week of acclimatization. The FA group served as control. The FA and UA mice were housed in the chambers with or without high efficiency particulate air filters, respectively. CA mice were housed in the chamber with the concentrated PM2.5 using the PM2.5 concentration enrichment system (Beijing Huironghe Technology Co., Ltd, Beijing, China) according to our previously reported procedures (Su et al., 2020). After the last day of exposure, all mice survived and were anesthetized with 3 % pentobarbital sodium, then sacrificed, and lung tissues were separated and weighed. Six random samples every group was applied for in vivo study."
item1937 "For the TAA (Sigma-Aldrich, USA) induced mouse liver fibrosis model, briefly, TAA (200 mg/kg, diluted in saline) was injected 3 times weekly for 8 weeks. Mice were sacrificed 24 h after the last administration."
item1938 .
item1939 20 adult male Wistar rats (180-200 g) were purchased from the Guangdong Provincial Center for Disease Control and Prevention. A 12 h/12 h light/dark cycle was maintained for the animals and they were fed standard food pellets and water as needed.
item1940 20 adult male Wistar rats (180-200 g) were purchased from the Guangdong Provincial Center for Disease Control and Prevention. A 12 h/12 h light/dark cycle was maintained for the animals and they were fed standard food pellets and water as needed.
item1941 "The nude mice were randomly assigned to two groups consisting of six mice each. We injected transformed cells (P0 and P40) into the flank of each mouse in 0.1 mL of sterile PBS to form xenograft tumors. The tumor volume was measured every 2 or 3 days (volume = length × width2 × 1/2). The tumors were resected, imaged, and weighed after the mice were sacrificed. One piece of each tumor tissue was fixed in 4% (v/v) paraformaldehyde for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, and the remaining tissue was stored at -80 °C."
item1942 "The nude mice were randomly assigned to two groups consisting of six mice each. We injected transformed cells (P0 and P40) into the flank of each mouse in 0.1 mL of sterile PBS to form xenograft tumors. The tumor volume was measured every 2 or 3 days (volume = length × width2 × 1/2). The tumors were resected, imaged, and weighed after the mice were sacrificed. One piece of each tumor tissue was fixed in 4% (v/v) paraformaldehyde for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, and the remaining tissue was stored at -80 °C."
item1943 "The nude mice were randomly assigned to two groups consisting of six mice each. We injected transformed cells (P0 and P40) into the flank of each mouse in 0.1 mL of sterile PBS to form xenograft tumors. The tumor volume was measured every 2 or 3 days (volume = length × width2 × 1/2). The tumors were resected, imaged, and weighed after the mice were sacrificed. One piece of each tumor tissue was fixed in 4% (v/v) paraformaldehyde for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, and the remaining tissue was stored at -80 °C."
item1944 "Mice were intraperitoneally injected with CL316,243(Sigma) at 1 mg/kg/day for seven consecutive days. To exclude the BAT function, interscapular BAT was surgically removed37,38. The OCR of adipose tissues was performed using Clark-type oxygen electrodes (Strathkelvin Instruments)."
item1945 "Adult, male C57BL/6J mice (9-10 weeks old, 20-25 g) supplied from the Animal Resources Center were used for experiments. Mice were housed 4 animals per cage on a 12 h light:dark cycle (lights on 0700 h) in a humidity- and temperature-controlled vivarium, with rodent chow and water provided ad libitum."
item1946 "Adult, male C57BL/6J mice (9-10 weeks old, 20-25 g) supplied from the Animal Resources Center were used for experiments. Mice were housed 4 animals per cage on a 12 h light:dark cycle (lights on 0700 h) in a humidity- and temperature-controlled vivarium, with rodent chow and water provided ad libitum."
item1947 .
item1948 .
item1949 .
item1950 "METTL3 and TNC overexpression model was achieved by in situ injection of overexpression AAV9 into the heart of C57/BL mice, and the injected virus titers were 1.8 × 1012 PFU/mL. The stable overexpression of METTL3 and overexpression of TNC could be obtained after 3 weeks. The overexpression AAV9 was purchased from Hanheng Biotechnology Co., Ltd. (Shanghai, China). The vector was HBAAV2/9-CMV-m-METTL3-3xflag-Null and HBAAV2/9-CMV-m-TNC-3xflag-Null."
item1951 .
item1952 .
item1953 "Nude mice (6 weeks, female) were injected with shNC, shMETTL16, shMETTL16 + shNC, and shMETTL16 + shVPS33B HOS cells to induce subcutaneous tumor formation randomly (n = 6 per group)."
item1954 "Four weeks old female BALB/c nude mice were purchased from SJA Laboratory Animal Co, Ltd (Changsha, China), and acclimated in a specific pathogen free environment for 1 week. SKOV3/DDP cells treated with shBIRC5, or shBIRC5 combined with pcDNA-IGF2BP1 plasmid (IGF2BP1) or corresponding negative control were suspended in 100 μL McCoy's 5A medium and implanted subcutaneously into the left frank of mice in 1 × 107 cells/mice. After 7 days incubation, three groups of mice received 10 mg/kg cisplatin respectively, sacrificing and tumor collecting after 30 days."
item1955 "Four weeks old female BALB/c nude mice were purchased from SJA Laboratory Animal Co, Ltd (Changsha, China), and acclimated in a specific pathogen free environment for 1 week. SKOV3/DDP cells treated with shBIRC5, or shBIRC5 combined with pcDNA-IGF2BP1 plasmid (IGF2BP1) or corresponding negative control were suspended in 100 μL McCoy's 5A medium and implanted subcutaneously into the left frank of mice in 1 × 107 cells/mice. After 7 days incubation, three groups of mice received 10 mg/kg cisplatin respectively, sacrificing and tumor collecting after 30 days."
item1956 "Adult male Sprague-Dawley rats (body weight 220 g-250 g) were acquired from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China, license No. SCXK (XIANG) 2016-0002). They were housed under specific pathogen-free conditions in the Department of Laboratory Animals, Central South University (Changsha, China, license No. SYXK (XIANG) 2015-0017). All rats were kept in air-conditioned animal quarters under standard conditions (50 ± 10% relative humidity, 12-h light/dark cycle, 22 ± 2 °C), with ad libitum water and food."
item1957 "All experiments were performed in 10-wk-old male mice unless otherwise indicated. Mice carrying a floxed Ythdf2 allele [24] and the alpha-MHC Cre Ribo-tag mouse [21] were crossed to obtain a cardiomyocyte specific Ythdf2 KO mouse expressing a HA-tagged RPL22. The mice were housed in a temperature- and humidity-controlled facility with a 12-h light-dark cycle. At 10 wk. of age, male mice underwent TAC (27 gauge needle) or sham operation, as previously described [25]. For echocardiography, the mice were anesthetized with 2% isoflurane and scanned using a Vevo2100 imaging system (Visual Sonics) as previously described [46]. Institutional Animal Care and Use Committee approval was obtained for all animal studies."
item1958 .
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item1963 .
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item1969 "Indicated HCC cells were intrasplenically injected into 5-week-old male BALB/C athymic nude mice (SpePharm Biotechnology, Beijing, China). After being fed in specific pathogen free condition for 35 days, the mice were euthanized and the livers were resected and subjected to HE staining."
item1970 "Indicated HCC cells were intrasplenically injected into 5-week-old male BALB/C athymic nude mice (SpePharm Biotechnology, Beijing, China). After being fed in specific pathogen free condition for 35 days, the mice were euthanized and the livers were resected and subjected to HE staining."
item1971 "Indicated HCC cells were intrasplenically injected into 5-week-old male BALB/C athymic nude mice (SpePharm Biotechnology, Beijing, China). After being fed in specific pathogen free condition for 35 days, the mice were euthanized and the livers were resected and subjected to HE staining."
item1972 "To induce tumor formation, 5 × 106 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice (Nanjing Biomedical Research Institute, Nanjing University). Tumor growth was assessed each week. The mice were sacrificed 4 weeks after surgery. Tumor volume was calculated as follows: tumor volume = length × width2 × 0.52. For generating the cancer metastasis model, we injected 1 × 106 cells into 5-week-old nude mice by tail vein injection and observed lung metastases after six weeks. The AAVdCasRx-ALKBH5-CD and AAVITGA6-multi-gRNA vectors were packaged into AAV serotype 8 viruses by Han Heng Biotechnology (Shanghai). Next, 5 × 105 T24 cells were subcutaneously injected into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice. After 1 week, the mice were randomly divided into two groups:AAV-Ctrl and AAV-dCasRx-ALKBH5. In the control group, mice were intratumorally injected with 1 × 1011 vp AAVGFP every second day a total of three times. In the experimental group, mice were injected with AAVdCas Rx-ALKBH5-CD and AAVITGA6-multi-gRNA using the same approach. Tumor weight and size were measured after 30 days. The livers and kidneys were collected for pathological analysis."
item1973 "To induce tumor formation, 5 × 106 cells were subcutaneously implanted into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice (Nanjing Biomedical Research Institute, Nanjing University). Tumor growth was assessed each week. The mice were sacrificed 4 weeks after surgery. Tumor volume was calculated as follows: tumor volume = length × width2 × 0.52. For generating the cancer metastasis model, we injected 1 × 106 cells into 5-week-old nude mice by tail vein injection and observed lung metastases after six weeks. The AAVdCasRx-ALKBH5-CD and AAVITGA6-multi-gRNA vectors were packaged into AAV serotype 8 viruses by Han Heng Biotechnology (Shanghai). Next, 5 × 105 T24 cells were subcutaneously injected into 5-week-old BALB/cJNju-Foxn1nu/Nju nude mice. After 1 week, the mice were randomly divided into two groups:AAV-Ctrl and AAV-dCasRx-ALKBH5. In the control group, mice were intratumorally injected with 1 × 1011 vp AAVGFP every second day a total of three times. In the experimental group, mice were injected with AAVdCas Rx-ALKBH5-CD and AAVITGA6-multi-gRNA using the same approach. Tumor weight and size were measured after 30 days. The livers and kidneys were collected for pathological analysis."
item1974 "Twenty male healthy C57BL/6J mice (10 weeks) were purchased from the National Rodent Laboratory Animal Resources Center (Shanghai, China). All mice were raised and treated in accordance with the procedures set by the Animal Resource Center of our School of Medicine. We referred to the experiment of Xu and Xu. 32 Fifteen mice were randomly selected for medial meniscus (DMM) resection to establish mice models of OA knee joint. Sham operation was performed on mice in sham group. Two weeks after DMM operation, 10 mice were randomly selected and divided into 2 groups. Mice in both groups were injected weekly with 5 μg sh-METTL3 or sh-NC (GenePharma). Six weeks after surgery, knee joint specimens were collected for analysis."
item1975 "Adult male Sprague-Dawley (150-200 g in body weight) rats were randomly divided into control or MCT/vehicle rats. Experimental rats were administered an intraperitoneal injection of monocrotaline (60 mg/kg, Sigma, 315-22-0), and their littermates were injected with saline. For GSK2606414 treatment, rats that underwent monocrotaline treatment were treated either with vehicle or GSK2606414 (10 mg/kg, Sigma, 1,337,531-89-1) by intraperitoneal injection per day. GSK2606414 was dissolved in a mixture of dimethyl sulfoxide (DMSO): polyethylene glycol (PEG) 400: distilled water (1: 4: 5). After 4 weeks, RV systolic blood pressure (RVSP) was measured with pressure transducers under anesthesia. The RV hypertrophy was analyzed as a ratio of RV to left ventricular and septal weight. Left lung tissues were fixed in 4% paraformaldehyde solution for the following histology staining; right lung tissues and pulmonary arteries were excised and immediately frozen in liquid nitrogen for other experiments."
item1976 "Adult male Sprague-Dawley (150-200 g in body weight) rats were randomly divided into control or MCT/vehicle rats. Experimental rats were administered an intraperitoneal injection of monocrotaline (60 mg/kg, Sigma, 315-22-0), and their littermates were injected with saline. For GSK2606414 treatment, rats that underwent monocrotaline treatment were treated either with vehicle or GSK2606414 (10 mg/kg, Sigma, 1,337,531-89-1) by intraperitoneal injection per day. GSK2606414 was dissolved in a mixture of dimethyl sulfoxide (DMSO): polyethylene glycol (PEG) 400: distilled water (1: 4: 5). After 4 weeks, RV systolic blood pressure (RVSP) was measured with pressure transducers under anesthesia. The RV hypertrophy was analyzed as a ratio of RV to left ventricular and septal weight. Left lung tissues were fixed in 4% paraformaldehyde solution for the following histology staining; right lung tissues and pulmonary arteries were excised and immediately frozen in liquid nitrogen for other experiments."
item1977 "Adult male Sprague-Dawley (150-200 g in body weight) rats were randomly divided into control or MCT/vehicle rats. Experimental rats were administered an intraperitoneal injection of monocrotaline (60 mg/kg, Sigma, 315-22-0), and their littermates were injected with saline. For GSK2606414 treatment, rats that underwent monocrotaline treatment were treated either with vehicle or GSK2606414 (10 mg/kg, Sigma, 1,337,531-89-1) by intraperitoneal injection per day. GSK2606414 was dissolved in a mixture of dimethyl sulfoxide (DMSO): polyethylene glycol (PEG) 400: distilled water (1: 4: 5). After 4 weeks, RV systolic blood pressure (RVSP) was measured with pressure transducers under anesthesia. The RV hypertrophy was analyzed as a ratio of RV to left ventricular and septal weight. Left lung tissues were fixed in 4% paraformaldehyde solution for the following histology staining; right lung tissues and pulmonary arteries were excised and immediately frozen in liquid nitrogen for other experiments."
item1978 .
item1979 .
item1980 "Pregnant rats were randomly assigned to 5 groups: normal pregnancy (normal) group, PE group, circSETD2 overexpression (PE + oe-circSETD2) group, METTL3 overexpression (PE + oe-METTL3) group, overexpression control (PE + oe-NC) group, joint treatment (PE + oe-METTL3 + si-circSETD2) group, and joint treatment control (PE + oe-METTL3 + si-NC) group, with 6 rats in each group. After continuous injection of L-NAME for 4 days, the rats in the overexpression and overexpression control groups were further injected with 30 μl lentivirus vector oe-circSETD2, oe-METTL3 or oe-NC solution (GenePharma, Shanghai, China) through the tail vein; after continuous injection of L-NAME for 4 days, the rats in the PE + oe-METTL3 + si-circSETD2 group were further injected with 30 μl lentiviral vector oe-circSETD2 and si-circSETD2 (GenePharma) at the virus titer of 5 × 107 PFU/mL. The rats were euthanized on the 16th day of pregnancy. Then, the chorionic trophoblast tissues were isolated from the placenta of pregnant rats and divided into 2 parts, with 1 part for extraction and detection of tissue RNA, and the other part fixed in the buffer containing 10% formalin, embedded in paraffin, and made into 5 μm sections."
item1981 "Pregnant rats were randomly assigned to 5 groups: normal pregnancy (normal) group, PE group, circSETD2 overexpression (PE + oe-circSETD2) group, METTL3 overexpression (PE + oe-METTL3) group, overexpression control (PE + oe-NC) group, joint treatment (PE + oe-METTL3 + si-circSETD2) group, and joint treatment control (PE + oe-METTL3 + si-NC) group, with 6 rats in each group. After continuous injection of L-NAME for 4 days, the rats in the overexpression and overexpression control groups were further injected with 30 μl lentivirus vector oe-circSETD2, oe-METTL3 or oe-NC solution (GenePharma, Shanghai, China) through the tail vein; after continuous injection of L-NAME for 4 days, the rats in the PE + oe-METTL3 + si-circSETD2 group were further injected with 30 μl lentiviral vector oe-circSETD2 and si-circSETD2 (GenePharma) at the virus titer of 5 × 107 PFU/mL. The rats were euthanized on the 16th day of pregnancy. Then, the chorionic trophoblast tissues were isolated from the placenta of pregnant rats and divided into 2 parts, with 1 part for extraction and detection of tissue RNA, and the other part fixed in the buffer containing 10% formalin, embedded in paraffin, and made into 5 μm sections."
item1982 .
item1983 .
item1984 .
item1985 "Male Sprague-Dawley rats (8-10 weeks old) weighing 200-250 g were purchased from Guangdong Medical Experimental Animal Cente [License No. SCXX (Guangdong) 2018-0002]. Animals were hosted in a centralized location in the Experimental Animal Center of the Third People's Hospital of Shenzhen [License No. SYXK (Guangdong) 2017-0120], with a standardized circadian cycle of 12 h for light and darkness at 20-25 °C and 55-60% humidity. Food pellets and water were provided ad libitum in specific-pathogen-free cages. All rats entered the experiment after 7 days of quarantine in the animal room without any abnormalities (normal activity and weight, normal gait, etc.). Thirty-four rats were randomly divided into 4 groups: 1 Sham operation group (Sham group, n = 7): only the right sciatic nerve trunk was exposed without ligation; 2 Chronic constriction injury (CCI) model group (NPP group, n = 7): the right sciatic nerve was exposed and ligated; 3 Intrathecal injection of AAV-METTL3 shRNA + CCI model group( M3 + NPP group, n = 10): AAV-METTL3 shRNA was intrathecally injected, and the right sciatic nerve was ligated after 19 days; 4 Intrathecal injection of negative control virus + CCI model group (Scr + NPP group, n = 10): AAV-scrambled shRNA was intrathecally injected, and the right sciatic nerve was ligated 19 days later. This experiment has been approved by the Animal Ethics Committee of the Third People's Hospital of Shenzhen (IACUC: 2019019), and strictly follows the 3 R principle of reduction, refinement and replacement in animal experiments."
item1986 .
item1987 .
item1988 .
item1989 "Twelve 6-week-old BALB/c male nude mice (Vitalriver, Beijing, China) were randomly divided to two groups. One group nude mice were subcutaneously injected with MG63 cells of YTHDF3 knockdown (sh-YTHDF3) or controls (sh-NC) at the right flank. The mice xenografts had been approved by the Laboratory Animal Welfare & Ethics Committee of Xi'an Jiao Tong University. All these animal procedures and handling care were performed in accordance with the Health guide for the care National Institutes and Laboratory animals."
item1990 "As-transformed cells (1 × 107) and control cells (1 × 107) in Matrigel (Corning, USA) were injected subcutaneously into the right flanks of male athymic nude mice (6 replicates per group) obtained from Huafukang Co., Ltd (Beijing, China) at four weeks of age. The volumes of tumors were monitored weekly. Subcutaneous tissues at the injection sites were acquired from the nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for one week, and a pathological examination was conducted. The subcutaneous tissues at the injection sites were acquired from nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for 12 weeks. These tissues were used for reculturing cells in vitro. The medical ethics committee of Sichuan University has approved all animal procedures (Approval number: K2021011)."
item1991 "As-transformed cells (1 × 107) and control cells (1 × 107) in Matrigel (Corning, USA) were injected subcutaneously into the right flanks of male athymic nude mice (6 replicates per group) obtained from Huafukang Co., Ltd (Beijing, China) at four weeks of age. The volumes of tumors were monitored weekly. Subcutaneous tissues at the injection sites were acquired from the nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for one week, and a pathological examination was conducted. The subcutaneous tissues at the injection sites were acquired from nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for 12 weeks. These tissues were used for reculturing cells in vitro. The medical ethics committee of Sichuan University has approved all animal procedures (Approval number: K2021012)."
item1992 "As-transformed cells (1 × 107) and control cells (1 × 107) in Matrigel (Corning, USA) were injected subcutaneously into the right flanks of male athymic nude mice (6 replicates per group) obtained from Huafukang Co., Ltd (Beijing, China) at four weeks of age. The volumes of tumors were monitored weekly. Subcutaneous tissues at the injection sites were acquired from the nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for one week, and a pathological examination was conducted. The subcutaneous tissues at the injection sites were acquired from nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for 12 weeks. These tissues were used for reculturing cells in vitro. The medical ethics committee of Sichuan University has approved all animal procedures (Approval number: K2021013)."
item1993 "As-transformed cells (1 × 107) and control cells (1 × 107) in Matrigel (Corning, USA) were injected subcutaneously into the right flanks of male athymic nude mice (6 replicates per group) obtained from Huafukang Co., Ltd (Beijing, China) at four weeks of age. The volumes of tumors were monitored weekly. Subcutaneous tissues at the injection sites were acquired from the nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for one week, and a pathological examination was conducted. The subcutaneous tissues at the injection sites were acquired from nude mice after the injection of As-transformed cells (3 replicates) and control cells (3 replicates) for 12 weeks. These tissues were used for reculturing cells in vitro. The medical ethics committee of Sichuan University has approved all animal procedures (Approval number: K2021014)."
item1994 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
item1995 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
item1996 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
item1997 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21383)."
item1998 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21384)."
item1999 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21385)."
item2000 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
item2001 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
item2002 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
item2003 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21383)."
item2004 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21384)."
item2005 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21385)."
item2006 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
item2007 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
item2008 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
item2009 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21383)."
item2010 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21384)."
item2011 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21385)."
item2012 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
item2013 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
item2014 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
item2015 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21383)."
item2016 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21384)."
item2017 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21385)."
item2018 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
item2019 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
item2020 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
item2021 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21383)."
item2022 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21384)."
item2023 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21385)."
item2024 .
item2025 .
item2026 "Indicated HCC cells were subcutaneously inoculated into the flank of 5-week-old male BALB/C athymic mice, which were purchased from Shanghai SLAC Laboratory Animal Co. and fed in specific pathogen-free condition. Subcutaneous tumor volumes were measured every week and calculated using the formula 0.5×length×width2. At the 28th day after inoculation, subcutaneous tumors were resected, weighed, and photographed. This study was approved by Affiliated Hospital of Youjiang Medical University for Nationalities Institutional Review Board."
item2027 .
item2028 .
item2029 .
item2030 .
item2031 "The in vivo experiments were conducted in line with a previous report with some modifications [29]. Male BALB/c nude mice (6-week-old, n=48) were bought from Cavens (Changzhou, China) and housed in a spe cific pathogen-free condition. All mice were given a free diet with an alternating 12 h light/dark cycle. All mice were acclimated for 5 days prior to the commencement of the experiments. Then, the mice were divided into four groups: CRC group (n=12), shNC group (n=12), shPDK4 group (n=12), and shPDK4+shWTAP group (n=12). Mice in the CRC group received no treatment, while those in the other three groups received a subcutaneous injection of 1×106 HCT116 cells (for six mice) and HT-29 cells (for six mice). Moreover, mice received the intervention with shNC in shNC group, shPDK in shPDK group, and shPDK and shWTAP together in the shPDK + shWTAP group. Then, the mice were normally reared for 4 weeks, and the tumor volume was calculated eve ry week as per the formula (volume = 3.14 × tumor length× tumor width2/6)."
item2032 "Male C57BL/6 mice (25-30 g) were obtained from Vital River Laboratory Animal Technology (Beijing, China) and were provided adaptive feeding for a week at the suitable temperature and humidity. All animals were housed in micro-isolator cages with free access to food and water according to the Guide for the Care and Use of Laboratory Animals. The myocardial I/R operation were followed by previous research (Song et al., 2015). The mice were randomly divided into myocardial I/R group (n = 10) and sham group (n = 10). Mice were anesthetized (50 mg/kg pentobarbital sodium, intraperitoneal injection) before assays. The supine of mice were fixed on the operating table connected with the standard lead II electrocardiogram. The left thorax was cut to expose the heart and the left anterior descending (LAD) coronary artery was ligated by 7/0 sterile suture. Myocardial ischemia was induced by LAD ligation for 30 min followed by 120 min of reperfusion. Sham group mice underwent the same surgical procedures without LAD coronary artery ligation. After assay, the surviving animals were transferred to institution's animal department for euthanizing mice."
item2033 "C57/BL6 male mice (eight per group) were randomly divided into four groups: basal CD group, HFD + 0.9% NaCl injection group, HFD + DMSO injection group, and HFD + STM2457 (Catalog No. 2499663-01-1, Selleck Chemicals, Houston, Texas) injection group. When the mean body weight of mice in HFD group was statistically different from that of mice in the control group [(mean body weight in HFD:mean body weight in CD)/mean body weight in CD > 30%], the treatment was started once a day for one or two weeks. STM2457 was administered at a dose of 50 mg/kg and weighed prior to each injection. DMSO or saline was also administered at an equal volume. Body weight was recorded every day. Injection of STM2457 was conducted daily and totally for one or two weeks."
item2034 "Bmi1CreER and RosatdTomato mice were obtained from The Jackson Laboratory, while Mettl3flox/flox and Mettl3KI/KI mice were provided from the laboratory of Prof. Quan Yuan at the West China Hospital of Stomatology. As previously described, we treated mice with 100 μg/mL 4-nitroquinoline 1-oxide (4NQO) (Sigma, N8141) to induce HNSCC formation. In brief, 6-week-old mice were fed with 4NQO for 16 weeks and then normal water for another 10 weeks. To lineage tracing and conditional knockout and knockin mouse model, tamoxifen (1 mg/mouse/day for 3 days; Sigma, T5648-1G) was injected intraperitoneally after 4NQO treatment. Then, we collected tongue samples, made frozen sections, and observed sections under a fluorescence microscope to calculate the positive percentage of BMI1 cells.+."
item2035 "Bmi1CreER and RosatdTomato mice were obtained from The Jackson Laboratory, while Mettl3flox/flox and Mettl3KI/KI mice were provided from the laboratory of Prof. Quan Yuan at the West China Hospital of Stomatology. As previously described, we treated mice with 100 μg/mL 4-nitroquinoline 1-oxide (4NQO) (Sigma, N8141) to induce HNSCC formation. In brief, 6-week-old mice were fed with 4NQO for 16 weeks and then normal water for another 10 weeks. To lineage tracing and conditional knockout and knockin mouse model, tamoxifen (1 mg/mouse/day for 3 days; Sigma, T5648-1G) was injected intraperitoneally after 4NQO treatment. Then, we collected tongue samples, made frozen sections, and observed sections under a fluorescence microscope to calculate the positive percentage of BMI1 cells.+."
item2036 .
item2037 .
item2038 "40 male Sprague Dawley (SD) rats (7-week-old) were obtained from SJA LABORATORY Animal Co, Ltd. (Hunan, China). After acclimation for 1 week, rats were randomly divided into four groups: the sham group, the sevoflurane group, the sevoflurane + Ad-NC group and the sevoflurane + Ad-YTHDF1 group, with ten animals in each group. Rats in sevoflurane groups were inhaled with 2.2% sevoflurane in the anesthetizing chamber at a flow rate of 1 L per minute for 6 h, and rats in the sham group were inhaled with 40% oxygen. The Ad-NC and Ad-YTHDF1 were constructed by GenePharma (Shanghai, China). Rats in the sevoflurane + Ad-NC group and the sevoflurane + Ad-YTHDF1 group were intracerebroventricularly injected with Ad-NC or Ad-YTHDF1 plasmids (100 μL; 1 × 109 PFU) by a Hamilton microsurgically gauged syringe into the left lateral cerebral ventricles 1 h before sevoflurane anesthesia."
item2039 .
item2040 .
item2041 .
item2042 "Ten specific pathogen-free (SPF) BALB/c nude mice (4-6 weeks old, 16 ± 2 g) were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. All experimental animals were kept in a SPF-level aseptic layer at 22-26 °C and 55 ± 5% humidity. Nude mice were randomly divided into sh-NC and sh-METTL3 groups, and these mice were anesthetized by intraperitoneal injection of pentobarbital sodium (60 mg/kg). After anesthesia, the right back skin of the nude mice was disinfected with conventional iodine. The mice in the sh-NC group were injected with 0.2 mL HEC-1-A cell suspension (1 × 106), and those in the sh-METTL3 group were injected with an equal volume of HEC-1-A cells that were stably transfected with sh-METTL3. The injector was slowly withdrawn, and the injection site was pressed for about 10 s to prevent an outflow of cell suspension. Three weeks later, the nude mice were euthanized by cervical dislocation, and subcutaneous tumors were separated and weighted. Tumor volume was measured as follows: tumor volume = 1/2 × length × width2."
item2043 "Ten specific pathogen-free (SPF) BALB/c nude mice (4-6 weeks old, 16 ± 2 g) were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. All experimental animals were kept in a SPF-level aseptic layer at 22-26 °C and 55 ± 5% humidity. Nude mice were randomly divided into sh-NC and sh-METTL3 groups, and these mice were anesthetized by intraperitoneal injection of pentobarbital sodium (60 mg/kg). After anesthesia, the right back skin of the nude mice was disinfected with conventional iodine. The mice in the sh-NC group were injected with 0.2 mL HEC-1-A cell suspension (1 × 106), and those in the sh-METTL3 group were injected with an equal volume of HEC-1-A cells that were stably transfected with sh-METTL3. The injector was slowly withdrawn, and the injection site was pressed for about 10 s to prevent an outflow of cell suspension. Three weeks later, the nude mice were euthanized by cervical dislocation, and subcutaneous tumors were separated and weighted. Tumor volume was measured as follows: tumor volume = 1/2 × length × width2."
item2044 "Ten specific pathogen-free (SPF) BALB/c nude mice (4-6 weeks old, 16 ± 2 g) were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. All experimental animals were kept in a SPF-level aseptic layer at 22-26 °C and 55 ± 5% humidity. Nude mice were randomly divided into sh-NC and sh-METTL3 groups, and these mice were anesthetized by intraperitoneal injection of pentobarbital sodium (60 mg/kg). After anesthesia, the right back skin of the nude mice was disinfected with conventional iodine. The mice in the sh-NC group were injected with 0.2 mL HEC-1-A cell suspension (1 × 106), and those in the sh-METTL3 group were injected with an equal volume of HEC-1-A cells that were stably transfected with sh-METTL3. The injector was slowly withdrawn, and the injection site was pressed for about 10 s to prevent an outflow of cell suspension. Three weeks later, the nude mice were euthanized by cervical dislocation, and subcutaneous tumors were separated and weighted. Tumor volume was measured as follows: tumor volume = 1/2 × length × width2."
item2045 "Ten specific pathogen-free (SPF) BALB/c nude mice (4-6 weeks old, 16 ± 2 g) were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. All experimental animals were kept in a SPF-level aseptic layer at 22-26 °C and 55 ± 5% humidity. Nude mice were randomly divided into sh-NC and sh-METTL3 groups, and these mice were anesthetized by intraperitoneal injection of pentobarbital sodium (60 mg/kg). After anesthesia, the right back skin of the nude mice was disinfected with conventional iodine. The mice in the sh-NC group were injected with 0.2 mL HEC-1-A cell suspension (1 × 106), and those in the sh-METTL3 group were injected with an equal volume of HEC-1-A cells that were stably transfected with sh-METTL3. The injector was slowly withdrawn, and the injection site was pressed for about 10 s to prevent an outflow of cell suspension. Three weeks later, the nude mice were euthanized by cervical dislocation, and subcutaneous tumors were separated and weighted. Tumor volume was measured as follows: tumor volume = 1/2 × length × width2."
item2046 "Approximately 1 × 106 cells with IGF2BP1 transfection plasmids were suspended in 100 μL PBS were injected in the flank of 4-weeks old male BALB/c nude mice. After 22 days observation, the tumor size was measured with vernier caliper and calculated using V = (width2 × length × 0.5), then, mice were anesthetized and sacrificed for tumors tissue gaining."
item2047 "After approval by Ethics Committee of School of Public Health, Southeast University, BALB/c nude mice (4-5 weeks old) were purchased and raised in SPF animal house. 1 × 107 HeLa cells or NC cells with stable overexpression of CARMN were prepared in each nude mouse. The mice were killed 3 weeks later, and tumor volume was measured."
item2048 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
item2049 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
item2050 .
item2051 "15 L of an empty vector-carrying lentivirus (5 × 107 copies/mL) was administered to the control group. The lentivirus as an in vivo delivery system can carry a luciferase transgene. The delivery system was injected into the marrow cavity of the femur 4 times at week 1, and luciferase expression in live mice was followed with a whole-animal fluorescence imaging system (IVIS Spectrum, USA) at 3 weeks post-injection."
item2052 "The Laboratory Animal Center of Soochow University provided female BALB/c nude mice (5 weeks old). Mice were kept under specific pathogen-free conditions. They were injected intravenously (i.v.) with FTO-overexpressing and control A549 cells (1.8 × 106 cells/mouse) to establish the in vivo model of NSCLC metastasis, and were then gavaged with dimethyl sulfoxide (DMSO) (25 mg/kg, daily) or the FAK inhibitor defactinib (VS6063) (Cat#S7654, Selleck, USA) (25 mg/kg, daily) beginning in the fifth week after injection. The mice were euthanized eight weeks after being inoculated, and their lungs were taken out and preserved in Bouin's solution for macroscopic investigation of metastatic nodules. Hematoxylin and eosin (H&E) staining of lung tissues was used to look for micrometastatic foci."
item2053 "The Laboratory Animal Center of Soochow University provided female BALB/c nude mice (5 weeks old). Mice were kept under specific pathogen-free conditions. They were injected intravenously (i.v.) with FTO-overexpressing and control A549 cells (1.8 × 106 cells/mouse) to establish the in vivo model of NSCLC metastasis, and were then gavaged with dimethyl sulfoxide (DMSO) (25 mg/kg, daily) or the FAK inhibitor defactinib (VS6063) (Cat#S7654, Selleck, USA) (25 mg/kg, daily) beginning in the fifth week after injection. The mice were euthanized eight weeks after being inoculated, and their lungs were taken out and preserved in Bouin's solution for macroscopic investigation of metastatic nodules. Hematoxylin and eosin (H&E) staining of lung tissues was used to look for micrometastatic foci."
item2054 "BALB/c nude mice (4-6 weeks) were randomly divided into four groups. Stable shRNA-NC, shIGF2BP3, shRNA-NC + siNF1 and shIGF2BP3 + siNF1 MDA-MB-231 cells were injected into each group of mice (1 × 107 cells/mouse). In addition, tumour volumes were recorded every 4 days."
item2055 "Nude (nu/nu) mice (4-5 weeks old) were purchased from Harlan Laboratories (Indianapolis, IN, USA). All animal studies were conducted in accordance with NIH animal use guidelines and a protocol approved by the UMMC Animal Care Committee. MIA PaCa-2 cells with LINC00901 overexpression or vector control (pCDH); LINC00901 KO or vector control (LCV2-m) at the exponential stage were harvested and mixed with 50% matrigel, and then injected into mice (2.5 million cells/spot) s.c. as described previously.21 Tumor growth was measured every 4 days, starting 2 weeks after injection of tumor cells."
item2056 "Nude (nu/nu) mice (4-5 weeks old) were purchased from Harlan Laboratories (Indianapolis, IN, USA). All animal studies were conducted in accordance with NIH animal use guidelines and a protocol approved by the UMMC Animal Care Committee. MIA PaCa-2 cells with LINC00901 overexpression or vector control (pCDH); LINC00901 KO or vector control (LCV2-m) at the exponential stage were harvested and mixed with 50% matrigel, and then injected into mice (2.5 million cells/spot) s.c. as described previously.21 Tumor growth was measured every 4 days, starting 2 weeks after injection of tumor cells."
item2057 .
item2058 "The 40 mice were randomly allocated into 4 groups (n = 10 per group): sham group, OVX group, shRNA-negative control (sh-NC) group, and sh-EGR1 group. Three days after the OVX operation, the sh-NC group and sh-EGR1 group were injected respectively with 100 μL sh-NC (1*1010 TU/mL) and 100 μL sh-EGR1 (1*1010 TU/mL) through tail veins, once a week for 4 weeks; the other groups were injected with an equal volume of phosphate-buffered saline (PBS). The adenoviral sh-NC and sh-EGR1 were procured from Hanbio (Shanghai, China). At the end of the 4th week, the orbital blood was collected and the serum was separated for detection of calcium ion, phosphorus, and alkaline phosphatase (ALP); the bilateral femurs and tibias were taken for micro-computed tomography (CT) and histopathological analysis."
item2059 "Male four-week-old BALB/C nude mice were inoculated subcutaneously in the right axilla with 1×106 HNRNPA2B1 knockout or negative control DU145 cells. Xenograft tumor growth was recorded weekly by measuring the width (W) and length (L) of the tumor with vernier calipers and calculating the tumor volume (V, mm3) using the formula V = W2 × L × 0.52. Xenograft tumors were harvested and weighed five weeks after cell injection."
item2060 "C57BL/6J mice (6-8 weeks old), purchased from Shanghai Slac Laboratory Animal Co., Ltd (Shanghai, China), were exposed to 0, 10, or 20 ppm NaAsO2 (Sigma, Japan) in drinking water for 6 months [23]. The arsenite concentration in high arsenic groundwater of Northern China is approximately 2 ppm, and the residents are mainly exposed to arsenite via drinking the water from artesian wells [51]. The dose of 20 ppm used in the present study was calculated according to factor (10) for animal-to-human extrapolation. Therefore, the arsenite concentrations used in our animal experiments are related to the human exposure level. As a positive control for pulmonary fibrosis, C57BL/6J mice were injected intraperitoneally with bleomycin (35 mg/kg) (Solarbio, China) twice weekly for 8 weeks. To downregulate, specifically, the levels of YTHDF1 in lung tissues, As-IPF AAV9-SP-Cp-shYTHDF1/AAV9-NC (Shanghai Genechem Co., Ltd, China) was intravenously injected at the 5th month of exposure of 20 ppm NaAsO2. Mice were housed on a 12-hour/12-hour light/dark cycle with free access to normal food."
item2061 "For the in vivo tumor formation assay, transfected A549 cells (1 × 107) were injected subcutaneously into the right thigh of female BALBc nude mice. One month after transplantation, the mice were sacrificed and the tissue samples were measured, weighed, and immunohistochemically analyzed. The tumor volume was calculated by the formula 1/2 × length × width2. Female BALB/c nude mice were purchased from Beijing SPF (Beijing, China) and were grouped randomly (5 or 6 mice per group)."
item2062 .
item2063 "Ythdc1flox/flox mice were maintained on a C57BL/6 background and normal chow. Ythdc1flox/flox mice were then crossed to a transgenic mouse line with MIP-CreERT [33]. MIP-CreERT mice have been described previously [15]. At the age of 10 weeks, both male and female mice were intraperitoneally injected with 100 mg/kg tamoxifen (T5648, Sigma) every other day for 3 times to delete Ythdc1 in beta-cells. Intraperitoneal glucose tolerance tests were performed on mice at age of 15 weeks after a 5-hour fast (2 g/kg dextrose). Insulin levels were measured at 0, 15 and 30 min after glucose challenge. Insulin tolerance tests were performed after a 5-hour fast by administering human recombinant insulin (0.75 U/kg)."
item2064 "Rats were randomly divided into 4 groups: sham group (6 rats), SNI model group (24 rats), SNI + vector group (6 rats), and SNI + pcDNA-D26496 group (6 rats). D26496 overexpressed Adeno associated virus (AAVs, virus titer: 1.88E + 13) and vector (OBiO Technology Corp., Shanghai, China) were injected into the epineurium of sciatic nerve of rats (5 μL for each) in the SNI + vector group and SNI + pcDNA-D26496 group, respectively."
item2065 "Rats were randomly divided into 4 groups: sham group (6 rats), SNI model group (24 rats), SNI + vector group (6 rats), and SNI + pcDNA-D26496 group (6 rats). D26496 overexpressed Adeno associated virus (AAVs, virus titer: 1.88E + 13) and vector (OBiO Technology Corp., Shanghai, China) were injected into the epineurium of sciatic nerve of rats (5 μL for each) in the SNI + vector group and SNI + pcDNA-D26496 group, respectively."
item2066 .
item2067 .
item2068 "K562 cells (1 × 106) were suspended in 100 μL of normal saline and the suspension was mixed with an equal volume of Matrigel. This mixture was subcutaneously injected into the right armpit of 4-week-old mice. Tumor size measurements were initiated on the day of inoculation. The tumor size was calculated using the formula: 0.5 × (long diameter) × (short diameter).2 When the tumor volume reached 100 mm3 (± 20%), rucaparib was given via intragastric administration at 50 mg/kg/d."
item2069 "K562 cells (1 × 106) were suspended in 100 μL of normal saline and the suspension was mixed with an equal volume of Matrigel. This mixture was subcutaneously injected into the right armpit of 4-week-old mice. Tumor size measurements were initiated on the day of inoculation. The tumor size was calculated using the formula: 0.5 × (long diameter) × (short diameter).2 When the tumor volume reached 100 mm3 (± 20%), rucaparib was given via intragastric administration at 50 mg/kg/d."
item2070 "K562 cells (1 × 106) were suspended in 100 μL of normal saline and the suspension was mixed with an equal volume of Matrigel. This mixture was subcutaneously injected into the right armpit of 4-week-old mice. Tumor size measurements were initiated on the day of inoculation. The tumor size was calculated using the formula: 0.5 × (long diameter) × (short diameter).2 When the tumor volume reached 100 mm3 (± 20%), rucaparib was given via intragastric administration at 50 mg/kg/d."
item2071 "K562 cells (1 × 106) were suspended in 100 μL of normal saline and the suspension was mixed with an equal volume of Matrigel. This mixture was subcutaneously injected into the right armpit of 4-week-old mice. Tumor size measurements were initiated on the day of inoculation. The tumor size was calculated using the formula: 0.5 × (long diameter) × (short diameter).2 When the tumor volume reached 100 mm3 (± 20%), rucaparib was given via intragastric administration at 50 mg/kg/d."
item2072 .
item2073 .
item2074 .
item2075 "To evaluate the effect of IGF2BP2 on the tumor formation ability in vivo, 2 × 106 cells were injected into the axilla of nude mice for subcutaneous tumor formation. After 9 days, modified IGF2BP2-si or si-NC was injected into the tumors every 3 days for 3 weeks. Tumor size was measured every 3 days."
item2076 "Animals were purchased from Changzhou Cavens Laboratory Animal Company (Changzhou, China) and maintained under specific pathogen-free (SPF) conditions. All animal experiments were performed in accordance with the guidelines of the Laboratory Animal Health Committee of Jiangsu University. Approximately 1 × 107 cells were injected subcutaneously into the dorsal surface of female BALB/c nude mice (n = 5 for each group, 4 weeks old, 15 ± 2 g). When the xenograft volume reached approximately 60 mm3 (after 1 week), DDP was intraperitoneally injected three times per week for 3 weeks. On Day 28, the mice were sacrificed and the tumors were frozen at -80 °C for follow-up experiments."
item2077 "Animals were purchased from Changzhou Cavens Laboratory Animal Company (Changzhou, China) and maintained under specific pathogen-free (SPF) conditions. All animal experiments were performed in accordance with the guidelines of the Laboratory Animal Health Committee of Jiangsu University. Approximately 1 × 107 cells were injected subcutaneously into the dorsal surface of female BALB/c nude mice (n = 5 for each group, 4 weeks old, 15 ± 2 g). When the xenograft volume reached approximately 60 mm3 (after 1 week), DDP was intraperitoneally injected three times per week for 3 weeks. On Day 28, the mice were sacrificed and the tumors were frozen at -80 °C for follow-up experiments."
item2078 "Four-week-old BALB/C-nu nude male mice were used for animal studies, and all animals were maintained in the specific pathogen-free (SPF) conditions at our institution. Huh-7 and stable YTHDC2 knockdown Huh-7 cells (approximately 1 × 107) resuspended with 50 μl of PBS and 50 μl of stromal gel were injected subcutaneously into the axilla of BALB/c nude mice to establish the subcutaneous xenograft model. When the volume of xenograft tumors up to 100 mm3, the mice were randomly divided into six groups, with five mice in each group. Erastin and sorafenib were dissolved in 10 % DMSO and 90 % corn oil and injected intraperitoneally into the mice every other day. After 28 days, mice were deeply anesthetized by intraperitoneal injection of sodium thiopental before decapitation, followed by tumors extraction, and stored in a -80 °C refrigerator for subsequent experiments. There were no occurrences of mouse mortality throughout the entire experimental period."
item2079 "Four-week-old BALB/C-nu nude male mice were used for animal studies, and all animals were maintained in the specific pathogen-free (SPF) conditions at our institution. Huh-7 and stable YTHDC2 knockdown Huh-7 cells (approximately 1 × 107) resuspended with 50 μl of PBS and 50 μl of stromal gel were injected subcutaneously into the axilla of BALB/c nude mice to establish the subcutaneous xenograft model. When the volume of xenograft tumors up to 100 mm3, the mice were randomly divided into six groups, with five mice in each group. Erastin and sorafenib were dissolved in 10 % DMSO and 90 % corn oil and injected intraperitoneally into the mice every other day. After 28 days, mice were deeply anesthetized by intraperitoneal injection of sodium thiopental before decapitation, followed by tumors extraction, and stored in a -80 °C refrigerator for subsequent experiments. There were no occurrences of mouse mortality throughout the entire experimental period."
item2080 HPSCC FaDu or Detroit 562 cells (1 × 106 cells) expressing vector control and construct lentiviruses were subcutaneously injected into the right flanks of 4-week-old male nude mice. Tumor diameters and body weight were recorded every 3 days for 1-6 weeks. Detroit 562 and FaDu cells (1 × 106 cells) were subcutaneously injected into the right flanks of 4-week-old male nude mice.
item2081 HPSCC FaDu or Detroit 562 cells (1 × 106 cells) expressing vector control and construct lentiviruses were subcutaneously injected into the right flanks of 4-week-old male nude mice. Tumor diameters and body weight were recorded every 3 days for 1-6 weeks. Detroit 562 and FaDu cells (1 × 106 cells) were subcutaneously injected into the right flanks of 4-week-old male nude mice.
item2082 HPSCC FaDu or Detroit 562 cells (1 × 106 cells) expressing vector control and construct lentiviruses were subcutaneously injected into the right flanks of 4-week-old male nude mice. Tumor diameters and body weight were recorded every 3 days for 1-6 weeks. Detroit 562 and FaDu cells (1 × 106 cells) were subcutaneously injected into the right flanks of 4-week-old male nude mice.
item2083 "Six-week-old female BALB/c nude mice were obtained from Peking University Animal Center (Beijing, China). These mice were randomized into experimental (N = 8 per group) and control groups (N = 8 per group). PC cells were resuspended in PBS (approximately 2 × 106 cells/100 μL) and subcutaneously inoculated into the left armpit of each nude mouse. The tumor length and width of the mice were monitored weekly. All mice were sacrificed 5 weeks postinjection, and tumors were excised and weighed."
item2084 .
item2085 .
item2086 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (Shanghai, China). 95D cells (1 × 107) transfected with sh-HNRNPA2B1 or sh-NC lentiviruses were injected subcutaneously into the right flanks of mice. After 8 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis."
item2087 "To perform in vivo plasmid transfection, the researchers mixed the pcDNA3.1-EGFP-METTL3 plasmid with in vivo jetPEI (Polyplus-transfection; Illkirch) per the manufacturer's guidelines. They mixed 40 μg of plasmid nucleic acid in 5% glucose (25 μl) with 6.4 μl of jetPEI solution in 5% glucose (25 μl) to obtain a final nitrogen and phosphorus ratio of 8. The mixture was incubated at room temperature for 30 minutes to form stable complexes before tracheal intubation of mice."
item2088 "To perform in vivo plasmid transfection, the researchers mixed the pcDNA3.1-EGFP-METTL3 plasmid with in vivo jetPEI (Polyplus-transfection; Illkirch) per the manufacturer's guidelines. They mixed 40 μg of plasmid nucleic acid in 5% glucose (25 μl) with 6.4 μl of jetPEI solution in 5% glucose (25 μl) to obtain a final nitrogen and phosphorus ratio of 8. The mixture was incubated at room temperature for 30 minutes to form stable complexes before tracheal intubation of mice."
item2089 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (Shanghai, China). 95D cells (1 × 107) transfected with sh-HNRNPA2B1 or sh-NC lentiviruses were injected subcutaneously into the right flanks of mice. After 8 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis."
item2090 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (Shanghai, China). 95D cells (1 × 107) transfected with sh-HNRNPA2B1 or sh-NC lentiviruses were injected subcutaneously into the right flanks of mice. After 8 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis."
item2091 "The width and length of tumors were recorded every 5 days and calculated as width [2] × length/2. After growth for 30 days, the mice were sacrificed and tumors were collected for further experiments."
item2092 "For the subcutaneous tumor model, cells from the treatment group and the control group (1 × 107) were subcutaneously injected into the right groin of BALB/c nude mice (4-6 weeks old, 18-20 g, 4 mice each). Both the treatment group and control group had 5 BALB/c nude mice each. After 21 days, all mice were sacrificed and tumor volume and weight were measured. For the pancreatic cancer in situ tumor model, mice were anesthetized with Avertin at a dose of 0.2 ml/10 g body weight, and 5 × 105 panc2 treatment group cells and control group cells were injected into the pancreas of mice respectively, and the abdomen was closed for disinfection. Both the treatment group and control group had 5 C57 mice each. After 21 days, the mice were sacrificed, the in-situ tumor was removed, and the volume and weight were measured for subsequent experiments."
item2093 "For the subcutaneous tumor model, cells from the treatment group and the control group (1 × 107) were subcutaneously injected into the right groin of BALB/c nude mice (4-6 weeks old, 18-20 g, 4 mice each). Both the treatment group and control group had 5 BALB/c nude mice each. After 21 days, all mice were sacrificed and tumor volume and weight were measured. For the pancreatic cancer in situ tumor model, mice were anesthetized with Avertin at a dose of 0.2 ml/10 g body weight, and 5 × 105 panc2 treatment group cells and control group cells were injected into the pancreas of mice respectively, and the abdomen was closed for disinfection. Both the treatment group and control group had 5 C57 mice each. After 21 days, the mice were sacrificed, the in-situ tumor was removed, and the volume and weight were measured for subsequent experiments."
item2094 .
item2095 "All animal experiments were performed in accordance with Institutional Animal Care and Use Committee guidelines. Six- to eight-week-old male DBA/1J mice (n = 5 per group) were purchased from the Shanghai Laboratory Animal Center, Chinese Academy of Science. All of mice were fed in the specific pathogen free-animal laboratory of the Experimental Animal Center of Fujian Medical University. Animals were housed under controlled conditions with a 12-hour light/dark cycle and with food/water access ad libitum."
item2096 .
item2097 .
item2098 .
item2099 "BALB nude mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. SKOV3 cells were transfected with sh-METTL3, sh-DDR2, DDR2 over-expression plasmid (oe-DDR2) or negative control. About one week later, the above SKOV3 cells were digested into single cells and subcutaneously injected to nude mice in different groups. After a duration of 4 weeks, the mice were euthanized by intraperitoneal injection of pentobarbital at a dosage 120 mg/kg. Finally, the subcutaneous tumors were removed to measure the size and volume (calculated according to volume = L (longest diameter) x W2 (shorter diameter)/2)."
item2100 "BALB nude mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. SKOV3 cells were transfected with sh-METTL3, sh-DDR2, DDR2 over-expression plasmid (oe-DDR2) or negative control. About one week later, the above SKOV3 cells were digested into single cells and subcutaneously injected to nude mice in different groups. After a duration of 4 weeks, the mice were euthanized by intraperitoneal injection of pentobarbital at a dosage 120 mg/kg. Finally, the subcutaneous tumors were removed to measure the size and volume (calculated according to volume = L (longest diameter) x W2 (shorter diameter)/2)."
item2101 .
item2102 .
item2103 .
item2104 .
item2105 "Stable cell clones, at a density of 5000 cells, were subcutaneously injected into the right flank of six-week-old male nude (nu/nu) mice (SLAC, Shanghai, China). These clones were infected with SKOV3-Nc and SKOV3-shALKBH5 in 100 uL of sterilized phosphate-buffered saline. Mice were allowed to recover for six weeks before reinjection. In each group, the tumor weights of six mice were measured and recorded. All mice were euthanized within six weeks post-surgery, following the removal of their tumors. Tumor sizes were measured using Vernier calipers, with the volume calculated as 1/2 length × width^2."
item2106 .
item2107 .
item2108 .
item2109 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice. The tumor volume was regularly monitored using the formula: volume = 0.5 × length × width2. After 5 weeks, the mice were euthanized and the weight of tumor was measured. In the lung metastasis models, which were conducted in nude mice, the groups consisted of 5 mice per group, similar to the tumor xenograft models. In these models, HeLa cells (2.0 × 106) that had been stably transfected were injected into the mice through the tail vein. After 5 weeks, the mice were euthanized. The lung tissues were excised, photographed, and fixed in 4% paraformaldehyde for H&E staining."
item2110 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice. The tumor volume was regularly monitored using the formula: volume = 0.5 × length × width2. After 5 weeks, the mice were euthanized and the weight of tumor was measured. In the lung metastasis models, which were conducted in nude mice, the groups consisted of 5 mice per group, similar to the tumor xenograft models. In these models, HeLa cells (2.0 × 106) that had been stably transfected were injected into the mice through the tail vein. After 5 weeks, the mice were euthanized. The lung tissues were excised, photographed, and fixed in 4% paraformaldehyde for H&E staining."
item2111 "The hepatic metastasis model was used to detect the function of METTL16 in vivo. BALB/c nude mice aged 4 weeks were divided into four groups randomly (n = 3). Subsequently, a longitudinal abdominal median incision was created after the mice were anesthetized. A total of 2.0 x 106 cells were injected into the spleen. Approximately 2.5 months later, liver tissues were harvested, imaged, and subjected to H&E staining."
item2112 "Female BALB/c nude mice (4-5 weeks old) were purchased from the Center of Experimental Animals of Guangdong. To establish a tail vein metastasis model, 2 × 106 SiHa cells in 200 μl PBS were injected into the tail vein of each mouse (n = 6 for both METTL3-overexpressing and empty vector groups). The mice were killed at approximately 8 weeks, and lung tissues were isolated and embedded in paraffin. Hematoxylin and eosin staining was then used to determine the number of lung metastasis nodules. To establish the popliteal lymph node metastasis model, 1 × 106 SiHa cells in 50 μl PBS were injected subcutaneously into the footpad of each mouse (n = 6 for both groups). Cells from the experimental and control groups were inoculated under the right and left footpads of each mouse, respectively. After 8 weeks, the popliteal lymph nodes were excised."
item2113 "Female BALB/c nude mice (4-5 weeks old) were purchased from the Center of Experimental Animals of Guangdong. To establish a tail vein metastasis model, 2 × 106 SiHa cells in 200 μl PBS were injected into the tail vein of each mouse (n = 6 for both METTL3-overexpressing and empty vector groups). The mice were killed at approximately 8 weeks, and lung tissues were isolated and embedded in paraffin. Hematoxylin and eosin staining was then used to determine the number of lung metastasis nodules. To establish the popliteal lymph node metastasis model, 1 × 106 SiHa cells in 50 μl PBS were injected subcutaneously into the footpad of each mouse (n = 6 for both groups). Cells from the experimental and control groups were inoculated under the right and left footpads of each mouse, respectively. After 8 weeks, the popliteal lymph nodes were excised."
item2114 .
item2115 .
item2116 .
item2117 .
item2118 "All mice were randomly categorized into 6 groups (n = 6 / group): the control group (mice were fed with normal drinking water), the DSS group, DSS + Lv-oe-NC group, DSS + Lv-oe-IGF2BP2 group, DSS + Lv-oe-IGF2BP2 + Lv-sh-NC group, and DSS + Lv-oe-IGF2BP2 + Lv-sh-GPX4 group. The last four groups of mice underwent anaesthesia via intraperitoneal injection of 30 mg/kg pentobarbital sodium. Next, mice were intracolonically administrated with Lv-oe-NC alone, Lv-oe-IGF2BP2 alone, Lv-oe-IGF2BP2 plus Lv-sh-NC, or Lv-oe-IGF2BP2 plus Lv-sh-GPX4 at a dose of 1 × 109 IU in a final volume of 100 μL PBS [18]. After 48 h, except for the control group, the acute UC model in mice was established by feeding with drinking water containing 3 % DSS for 7 days [19]. On the 10th day, all mice underwent euthanasia via inhalation of overdose CO2, after which the colons of mice in each group were collected, measured using a ruler."
item2119 .
item2120 "Nude mice were assigned randomly into two groups. For the first group, 2.5×106 A549 cells were injected subcutaneously into the right flank of mice, and 2.5×106 A549 cells mixed with 5×106 CAFs were injected into the left flank. For the second group, 2.5×106 A549-METTL3-KD cells were injected into the right flank, and A549-METTL3-KD cells mixed with 5×106 CAFs were injected into the left flank. Tumor volumes were measured every week."
item2121 "NOZ cells (2 × 106 cells) transfected with sh-IGF2BP3 or sh-NC were injected into the flanks of mice (n = 5) according to previously described criteria [23]. After 2 weeks of injection, the tumor volume was measured every 3 days. The tumor volume was calculated by volume = 1 / 2 × long × width2. On day 26, we weighed the tumor."
item2122 "For the subcutaneous xenograft model, 1 × 106 tumor cells were resuspended in 100 μL of DMEM and then injected into the axilla of each mouse (n = 5 mice per group). The mice were sacrificed after 2 weeks, the tumor weight was recorded, and the tumor volume was calculated according to the following equation: volume = 1/2*(length × width2). After the experiment, the specimens were fixed with 4% formaldehyde. For the intrahepatic tumor implantation model, 1 × 106 tumor cells were resuspended in 100 μL of DMEM and then inoculated under the capsule of the right lobe of the liver (n = 5 mice per group). The mice were sacrificed after 4 weeks, and the tumors were removed and subjected to HE staining."
item2123 "For the subcutaneous xenograft model, 1 × 106 tumor cells were resuspended in 100 μL of DMEM and then injected into the axilla of each mouse (n = 5 mice per group). The mice were sacrificed after 2 weeks, the tumor weight was recorded, and the tumor volume was calculated according to the following equation: volume = 1/2*(length × width2). After the experiment, the specimens were fixed with 4% formaldehyde. For the intrahepatic tumor implantation model, 1 × 106 tumor cells were resuspended in 100 μL of DMEM and then inoculated under the capsule of the right lobe of the liver (n = 5 mice per group). The mice were sacrificed after 4 weeks, and the tumors were removed and subjected to HE staining."
item2124 .
item2125 "Male C57BL/6 mice (6-8 weeks, 20-22 g) were supplied by Hubei Provincial Center for Disease Control and Prevention (Wuhan, China) and housed in the observation room. The mice were fed and kept under strict conditions of 22-26 °C, a standard 12 h light/dark cycle and 50 ± 5% relative humidity for 7 days to adapt to the conditions. Before the interventions, the mice were anesthetized with Chloral hydrate and divided into two groups at three time points: control mice were intratracheally instilled with 50 μL of sterile phosphate-buffered saline (PBS), and the silica-exposed group contained 50 μL of 50 mg/mL (2.5 mg) silica particles. Then, the mice were sacrificed by anesthesia on 7, 28 and 84 days after silica treatment, and the lung tissues and bronchoalveolar lavage fluid (BALF) were harvested for further study."
item2126 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μl medium and 50 μl matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
item2127 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μl medium and 50 μl matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
item2128 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μl medium and 50 μl matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
item2129 .
item2130 .
item2131 .
item2132 .
item2133 .
item2134 .
item2135 "For the subcutaneous xenograft model, HEC108-derived cells (4 × 106 cells) were injected subcutaneously into seven-week-old female BALB/cAJcl-nu/nu mice. The tumor volume was measured 8 weeks after injection. For the intraperitoneal xenograft model, HEC108-derived cells (4 × 106 cells) were injected intraperitoneally, and the number of intraperitoneal tumors was quantified 4 weeks after injection."
item2136 "The mice were acclimatized and fed for one week. Then, they were randomly divided into two groups: sh-NC group and sh-SHMT2 group. Cells transfected with sh-NC or sh-SHMT2 were subsequently cultured routinely. Next, cells with logarithmic growth phase (1 ×106) were taken and injected to the right axilla of nude mice. After subcutaneous inoculation, the mice were observed for their mental status, activity, and tumor formation. The tumor volume was monitored every 4 days, and the mice were euthanized after 28 days. Tumor tissues were separated and weighed from nude mice. A portion of the dissected tumor tissue was fixed overnight in 4% paraformaldehyde, embedded in paraffin blocks and sectioned."
item2137 "The mice were acclimatized and fed for one week. Then, they were randomly divided into two groups: sh-NC group and sh-SHMT2 group. Cells transfected with sh-NC or sh-SHMT2 were subsequently cultured routinely. Next, cells with logarithmic growth phase (1 ×106) were taken and injected to the right axilla of nude mice. After subcutaneous inoculation, the mice were observed for their mental status, activity, and tumor formation. The tumor volume was monitored every 4 days, and the mice were euthanized after 28 days. Tumor tissues were separated and weighed from nude mice. A portion of the dissected tumor tissue was fixed overnight in 4% paraformaldehyde, embedded in paraffin blocks and sectioned."
item2138 "The mice were acclimatized and fed for one week. Then, they were randomly divided into two groups: sh-NC group and sh-SHMT2 group. Cells transfected with sh-NC or sh-SHMT2 were subsequently cultured routinely. Next, cells with logarithmic growth phase (1 ×106) were taken and injected to the right axilla of nude mice. After subcutaneous inoculation, the mice were observed for their mental status, activity, and tumor formation. The tumor volume was monitored every 4 days, and the mice were euthanized after 28 days. Tumor tissues were separated and weighed from nude mice. A portion of the dissected tumor tissue was fixed overnight in 4% paraformaldehyde, embedded in paraffin blocks and sectioned."
item2139 "The mice were acclimatized and fed for one week. Then, they were randomly divided into two groups: sh-NC group and sh-SHMT2 group. Cells transfected with sh-NC or sh-SHMT2 were subsequently cultured routinely. Next, cells with logarithmic growth phase (1 ×106) were taken and injected to the right axilla of nude mice. After subcutaneous inoculation, the mice were observed for their mental status, activity, and tumor formation. The tumor volume was monitored every 4 days, and the mice were euthanized after 28 days. Tumor tissues were separated and weighed from nude mice. A portion of the dissected tumor tissue was fixed overnight in 4% paraformaldehyde, embedded in paraffin blocks and sectioned."
item2140 "The mice were acclimatized and fed for one week. Then, they were randomly divided into two groups: sh-NC group and sh-SHMT2 group. Cells transfected with sh-NC or sh-SHMT2 were subsequently cultured routinely. Next, cells with logarithmic growth phase (1 ×106) were taken and injected to the right axilla of nude mice. After subcutaneous inoculation, the mice were observed for their mental status, activity, and tumor formation. The tumor volume was monitored every 4 days, and the mice were euthanized after 28 days. Tumor tissues were separated and weighed from nude mice. A portion of the dissected tumor tissue was fixed overnight in 4% paraformaldehyde, embedded in paraffin blocks and sectioned."
item2141 .
item2142 "Neonatal C57BL/6J, B6.129S4-Ccr2tm1Ifc/J (METTL3-/-), and the respective Wild-Type (WT) control mice were purchased from the Experimental Animal Center of Nanjing Medical University. Mice were divided into three groups: 1) C57BL/6J mice were exposed to 21% oxygen (normoxia, NRMX) for 14 days; 2) C57BL/6J, METTL3-/- and WT control mice were exposed to 80% O2 oxygen (hyperoxia, HYRX) for 28 days; 3) Mice were injected with 15 μg of 3-MA 30 min before hyperoxia exposure. The procedures were performed in accordance with ARRIVE guidelines."
item2143 "Tumors were established by subcutaneous injection (1 × 106 E0771 cells in 0.1 ml saline) into the flanks of the mice. After 12 days, tumors reached > 150 mm3 in size, and the mice were randomly allocated into four groups and treated with Nab-PTX (22.3 mg/kg body weight; i.p., every 3 days), Fulvestrant (2.5 mg/kg body weight; i.p., every 3 days), Nab-PTX combined with fulvestrant, or vehicle PBS as control. Tumor volume was measured every 3 days. Mice were euthanized when the tumor size reached approximately 2000 mm3. Tumor volume was calculated using the following formula: length × width2 × 0.5."
item2144 "For the subcutaneous implantation model, stable METTL3 KO HeLa cells or WT HeLa cells were injected subcutaneously into BALB/c-Nude mice (1× 107 cells/0.1 mL/mouse). For radiation models, 1×107 WT HeLa cells were injected into the backs of nude mice. They were randomly divided into 4 groups: control group, irradiation group, STM2457 group, and irradiation combined with STM2457 group. After the transplanted tumor reached approximately 5 mm in diameter, the mice were orally administered STM2457 (50 mg/kg body weight, p.o.) and/or control oil (0.1 mL/mouse, p.o.). Approximately half an hour later, the tumor tissue was exposed to 4-Gy irradiation. Tumor growth was observed, and data were recorded after inoculation. After 2 weeks, the mice were killed, and the tumors were resected and weighed. The experimental procedures and animal use were performed with approval of the Institutional Animal Care and Use Committee of the Laboratory Animal Center. Animal experiments were carried out according to the Control of Animal Laboratory of the Academy of Military Medical Sciences and the principles on animal works (IACUC-DWZX-2021-711)."
item2145 "Cerebral I/R injury was induced by MCAO/R surgery following previous method [10]. Rats were anesthetized by intraperitoneal injection of 2% sodium pentobarbital. After cutting the cervical skin of the rats with surgical scissors, the internal, external and common carotid arteries were separated. Then the proximal ends of the external and the common carotid arteries were ligated and the distal internal carotid artery was clipped. After cutting an incision 1 cm away from the bifurcation of the internal carotid artery with a surgical scissors, the left common carotid artery was slowly inserted into the internal carotid artery of the rats with sutures coated with silicone (head diameter 0.36±0.02 mm, Beijing Sunbio Biotech Co., Ltd., China) until sutures reached the bifurcation of the middle cerebral artery, and the wound was sutured after the sutures were fixed. After blocking blood flow for 1 h, the plug was removed to restore blood flow and reperfusion was performed. Other rats were selected for the Sham group, and the operation procedure was the same as above except for no ligation and insertion."
item2146 "Cerebral I/R injury was induced by MCAO/R surgery following previous method [10]. Rats were anesthetized by intraperitoneal injection of 2% sodium pentobarbital. After cutting the cervical skin of the rats with surgical scissors, the internal, external and common carotid arteries were separated. Then the proximal ends of the external and the common carotid arteries were ligated and the distal internal carotid artery was clipped. After cutting an incision 1 cm away from the bifurcation of the internal carotid artery with a surgical scissors, the left common carotid artery was slowly inserted into the internal carotid artery of the rats with sutures coated with silicone (head diameter 0.36±0.02 mm, Beijing Sunbio Biotech Co., Ltd., China) until sutures reached the bifurcation of the middle cerebral artery, and the wound was sutured after the sutures were fixed. After blocking blood flow for 1 h, the plug was removed to restore blood flow and reperfusion was performed. Other rats were selected for the Sham group, and the operation procedure was the same as above except for no ligation and insertion."
item2147 "Mice were acclimatised at the Centenary Institute Animal Facility for a minimum of 7 days after initial arrival. Prior to tumour injection, mice were anaesthetised by ketamine/xylazine by intraperitoneal injection. Subsequently, the fur surrounding the 4th mammary fat pad on the right was removed using the hair removal cream. MDA-MB-231 cells (5 × 106) in 100 μL of 1:1 HBSS:Matrigel were then injected subcutaneously into the 4th right mammary fat pad using 27 g insulin needles. Mice were injected intraperitoneally with the reversal atipamezole to improve the recovery from anaesthesia. Mice were monitored twice weekly by assessing body condition, measuring body weights and tumour sizes. The frequency of monitoring was increased to daily when tumours reached > 500 mm3 in size. Mice were killed when tumours reached > 1000 mm3 in size and the relevant organs were harvested for analysis."
item2148 .
item2149 .
item2150 .
item2151 .
item2152 .
item2153 "Cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
item2154 "Cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
item2155 .
item2156 .
item2157 "Five-week-old female BALB/c nude mice were purchased from the Cancer Institute of the Chinese Academy of Medical Science (Beijing, China). Mice were randomly divided into two groups (eight mice per group), and 3×106 H1299 or H1299-LV-FTO-KD cells were injected subcutaneously into the flank of each mouse. For each group, four mice were treated with PEITC (100 mg/kg) intraperitoneally every two days for 14 days beginning the fifth week after cell injection, while the other four mice were treated with PBS as a control. At the end of the experiment, mice were euthanized, and tumor tissues were dissected for further analysis. Tumor weights were measured, and volumes were calculated (V = D/2 × d2, V: volume; D: longitudinal diameter; d: latitudinal diameter)."
item2158 "All animal experiments were approved by the Institutional Animal Care and Use Committee of Southern Medical University. Breast cancer cells (5 × 106) were implanted into the subcutaneous axilla of the forelimb of 3-4-week-old BALB/c nude mice. Seven days after transplantation, the diameter of the tumors was measured, and the tumors were removed after three weeks."
item2159 "All animal experiments were approved by the Institutional Animal Care and Use Committee of Southern Medical University. Breast cancer cells (5 × 106) were implanted into the subcutaneous axilla of the forelimb of 3-4-week-old BALB/c nude mice. Seven days after transplantation, the diameter of the tumors was measured, and the tumors were removed after three weeks."
item2160 "A total of 5×106 cells expressing vector or circRBM33 (or sh-circRBM33) were subcutaneously injected into both back sides of nude mice. For drug treatment, mice bearing transplanted tumours were randomly divided into two groups when the tumour volume reached 100-150 mm3 and then treated with vehicle or 20 mg/kg enzalutamide (or 50 mg/kg darolutamide) orally for 28 days. Tumour volume was measured every 3 days by calculating 0.5×length×width2, and the tumour weight was finally measured after sacrifice."
item2161 "A total of 5×106 cells expressing vector or circRBM33 (or sh-circRBM33) were subcutaneously injected into both back sides of nude mice. For drug treatment, mice bearing transplanted tumours were randomly divided into two groups when the tumour volume reached 100-150 mm3 and then treated with vehicle or 20 mg/kg enzalutamide (or 50 mg/kg darolutamide) orally for 28 days. Tumour volume was measured every 3 days by calculating 0.5×length×width2, and the tumour weight was finally measured after sacrifice."
item2162 .
item2163 .
item2164 .
item2165 .
item2166 .
item2167 .
item2168 "Male BALB/c nude mice (5-6 weeks) were obtained from Slac Laboratory Animal Center (Shanghai, China) and maintained under pathogen-free conditions. PANC-1 cells (2 × 106 cells suspended in 100 μl PBS) transfected with circMYO1C knockdown (sh-circMYO1C) or controls (sh-NC) were subcutaneously injected into the flank of nude mice. One week later, the tumor size was measured every three days."
item2169 "Male BALB/c nude mice (5-6 weeks) were obtained from Slac Laboratory Animal Center (Shanghai, China) and maintained under pathogen-free conditions. PANC-1 cells (2 × 106 cells suspended in 100 μl PBS) transfected with circMYO1C knockdown (sh-circMYO1C) or controls (sh-NC) were subcutaneously injected into the flank of nude mice. One week later, the tumor size was measured every three days."
item2170 .
item2171 .
item2172 "For the tumor metastasis mouse model, 5-week-old C57BL/6 mice were randomly grouped and injected with 5 × 105 Control or shFTO stable MC38 cells via tail vein. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days). To detect lung metastasis, mice were killed 2 weeks after tumor cell injection. Lung tissues were harvested and fixed with 4% PFA for paraffin-embedded section and lung metastases were detected with Nikon microscopy. For tumor intraperitoneal mouse model, 2 × 106 Dox-shCtrl, Dox-shFTO stable HCT8/5-FU cells were injected into 5-week-old male BALB/C nude mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or FB23-2 (10 mg/kg every 2 days). For tumor intraperitoneal mouse model, 5 × 105 shCtrl, shFTO-1, shFTO-2 stable MC38 cells were injected into 5-week-old C57BL/6 mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days)."
item2173 "For the tumor metastasis mouse model, 5-week-old C57BL/6 mice were randomly grouped and injected with 5 × 105 Control or shFTO stable MC38 cells via tail vein. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days). To detect lung metastasis, mice were killed 2 weeks after tumor cell injection. Lung tissues were harvested and fixed with 4% PFA for paraffin-embedded section and lung metastases were detected with Nikon microscopy. For tumor intraperitoneal mouse model, 2 × 106 Dox-shCtrl, Dox-shFTO stable HCT8/5-FU cells were injected into 5-week-old male BALB/C nude mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or FB23-2 (10 mg/kg every 2 days). For tumor intraperitoneal mouse model, 5 × 105 shCtrl, shFTO-1, shFTO-2 stable MC38 cells were injected into 5-week-old C57BL/6 mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days)."
item2174 .
item2175 "Male BALB/c nude mice (6 weeks old) were used for xenograft experiments. A 150 μL-cell suspension containing 5 × 106 cells was injected s.c. into each nude mouse. Tumor volumes were measured every 4 days for 1 month. To evaluate chemosensitivity to gemcitabine, mice were i.p. injected with gemcitabine (50 mg/kg) every 4 days when the tumor volume reached 100 mm3.Male NOD/SCID mice (6 weeks old) were used for lung metastasis experiments. A 150 μL-cell suspension containing 5 × 105 cells was injected i.v. into the tail vein of each mouse. After 1 month, the lungs were harvested and photographed, and the number of metastatic lesions was calculated."
item2176 "BALB/c nude mice (4 weeks old, male) were purchased from Vital River Laboratory Animals and maintained under specific pathogen-free conditions. For in vivo tumor xenograft model establishment, 10 mice were randomly chosen and divided into two groups: the sh-NC and sh-HOTAIRM1 groups. Next, U87 cells (approximately 1 × 106/200 μl PBS) transfected with shRNAs (sh-HOTAIRM1 or sh-NC) were subcutaneously inoculated into the right frontal node of nude mice. Tumor growth was monitored once weekly. Mice were killed by cervical dislocation until the fifth week (35 days), and tumors were resected and measured for weight and volume."
item2177 "BALB/c nude mice (4 weeks old, male) were purchased from Vital River Laboratory Animals and maintained under specific pathogen-free conditions. For in vivo tumor xenograft model establishment, 10 mice were randomly chosen and divided into two groups: the sh-NC and sh-HOTAIRM1 groups. Next, U87 cells (approximately 1 × 106/200 μl PBS) transfected with shRNAs (sh-HOTAIRM1 or sh-NC) were subcutaneously inoculated into the right frontal node of nude mice. Tumor growth was monitored once weekly. Mice were killed by cervical dislocation until the fifth week (35 days), and tumors were resected and measured for weight and volume."
item2178 .
item2179 "We injected 8 × 105 HO8910 cells into the footpads of mice. The mice were randomized two weeks after tumor cell injection to receive either an ITGB1 blocking antibody (Santa, CA, USA) or an IgG isotype antibody twice per week for four weeks 22,23. Y15 (30 mg/kg) against p-FAK (Tyr397) and PBS as a control were intraperitoneally injected twice per week for four weeks in the FAK-treatment assays. The main tumors and popliteal LNs were harvested and fixed in paraformaldehyde after six weeks of tumor cell inoculation. LN volumes were calculated as follows: LN volume (mm3) = (length [mm]) × (width [mm])2 × 0.52. The formalin-fixed, paraffin-embedded samples were analysed using immunohistochemistry and haematoxylin and eosin (H&E) staining."
item2180 "We injected 8 × 105 HO8910 cells into the footpads of mice. The mice were randomized two weeks after tumor cell injection to receive either an ITGB1 blocking antibody (Santa, CA, USA) or an IgG isotype antibody twice per week for four weeks 22,23. Y15 (30 mg/kg) against p-FAK (Tyr397) and PBS as a control were intraperitoneally injected twice per week for four weeks in the FAK-treatment assays. The main tumors and popliteal LNs were harvested and fixed in paraformaldehyde after six weeks of tumor cell inoculation. LN volumes were calculated as follows: LN volume (mm3) = (length [mm]) × (width [mm])2 × 0.52. The formalin-fixed, paraffin-embedded samples were analysed using immunohistochemistry and haematoxylin and eosin (H&E) staining."
item2181 .
item2182 .
item2183 "The BALB/c nude mice (male, 4-6 weeks) were provided via Vital River Laboratory Animal Technology (Beijing, China). For xenograft tumor growth assay, 15 mice were randomly classified into 3 groups (n = 5/group), including control, sh-NC or sh-DGUOK-AS1 group. In sh-NC or sh-DGUOK-AS1 group, mice were subcutaneously injected with SK-MES-1 cells (3 × 106) stably transfected with sh-NC or sh-DGUOK-AS1; Moreover, mice were subcutaneously injected with non-transfected SK-MES-1 cells (3 × 106) as control group. The tumors were monitored every 7 days, and size was calculated by (length × width2)/2. Mice were euthanized after 4 weeks, and the tumors were collected and weighed, followed via collection for further analyses. Ki67 and CD31 levels were measured through immunohistochemistry staining using Ki67 (ab15580, 1:2000 dilution, Abcam) or CD31 (ab18298, 1:3000 dilution, Abcam) according to the protocols. For pulmonary metastatic assay, 3 × 106 SK-MES-1 cells stably transfected with sh-NC or sh-DGUOK-AS1 or non-transfected SK-MES-1 cells were intravenously injected into mice. Mice were sacrificed after 8 weeks, and lung specimens were collected, followed by hematoxylin-eosin (HE) staining assay for metastatic lesions in lung tissues. All experiments were approved via the Animal Ethics Committee of The Second Hospital of Shandong University."
item2184 "The BALB/c nude mice (male, 4-6 weeks) were provided via Vital River Laboratory Animal Technology (Beijing, China). For xenograft tumor growth assay, 15 mice were randomly classified into 3 groups (n = 5/group), including control, sh-NC or sh-DGUOK-AS1 group. In sh-NC or sh-DGUOK-AS1 group, mice were subcutaneously injected with SK-MES-1 cells (3 × 106) stably transfected with sh-NC or sh-DGUOK-AS1; Moreover, mice were subcutaneously injected with non-transfected SK-MES-1 cells (3 × 106) as control group. The tumors were monitored every 7 days, and size was calculated by (length × width2)/2. Mice were euthanized after 4 weeks, and the tumors were collected and weighed, followed via collection for further analyses. Ki67 and CD31 levels were measured through immunohistochemistry staining using Ki67 (ab15580, 1:2000 dilution, Abcam) or CD31 (ab18298, 1:3000 dilution, Abcam) according to the protocols. For pulmonary metastatic assay, 3 × 106 SK-MES-1 cells stably transfected with sh-NC or sh-DGUOK-AS1 or non-transfected SK-MES-1 cells were intravenously injected into mice. Mice were sacrificed after 8 weeks, and lung specimens were collected, followed by hematoxylin-eosin (HE) staining assay for metastatic lesions in lung tissues. All experiments were approved via the Animal Ethics Committee of The Second Hospital of Shandong University."
item2185 "The mice were randomly separated into four groups (n = 3-5): young mice group (YNG); naturally aging mice group (OLD); PBS-treated mice group (Con); D-gal-treated aging mice group (D-gal). YNG group was the directly purchased mice (8-week-old) actually. OLD group: mice bought continued to be raised in a specific pathogen-free facility (SPF) individually for 24 months. Mice in Con group were injected subcutaneously with phosphate buffered saline (PBS) solution formulated from powder, with the same volume as the D-gal group. Mice from D-gal group were made by injecting (D-gal, 500 mg/kg body weight) daily for 8 weeks, subcutaneously. All animals were kept under a standard environmental condition (25 ± 2 °C; 12-h light-dark cycle) and allowed free access to water and regular sterile chow diets. All animal studies were designed in accordance with ARRIVE guidelines and experiment protocols followed NIH guidelines."
item2186 .
item2187 .
item2188 "For the unilateral ureteral obstruction (UUO)-induced ON model, mice were subjected to permanent ureteral ligation of the left kidney ureter under aseptic and anaesthetic conditions, whereas sham-operated mice merely lacked ligation under equivalent conditions. The mice were sacrificed under anaesthesia at 3, 7 and 14 days postoperatively. For the ischemia/reperfusion (I/R)-induced renal fibrosis model, mice were recovered with blood supply after 42 min of bilateral renal artery clamping under both aseptic and anaesthetic conditions. These mice were sacrificed under anaesthesia at 7 and 14 days postoperatively. For drug treatment, isoforsythiaside was injected intraperitoneally at concentrations of 10, 25 and 50 mg/kg per day when the model was established. Similarly, the sham-operated group received an equivalent volume of saline intraperitoneally as a control. The harvested kidneys have been primarily used for morphological and histopathological observations and molecular biology studies."
item2189 "For the unilateral ureteral obstruction (UUO)-induced ON model, mice were subjected to permanent ureteral ligation of the left kidney ureter under aseptic and anaesthetic conditions, whereas sham-operated mice merely lacked ligation under equivalent conditions. The mice were sacrificed under anaesthesia at 3, 7 and 14 days postoperatively. For the ischemia/reperfusion (I/R)-induced renal fibrosis model, mice were recovered with blood supply after 42 min of bilateral renal artery clamping under both aseptic and anaesthetic conditions. These mice were sacrificed under anaesthesia at 7 and 14 days postoperatively. For drug treatment, isoforsythiaside was injected intraperitoneally at concentrations of 10, 25 and 50 mg/kg per day when the model was established. Similarly, the sham-operated group received an equivalent volume of saline intraperitoneally as a control. The harvested kidneys have been primarily used for morphological and histopathological observations and molecular biology studies."
item2190 .
item2191 .
item2192 .
item2193 "Briefly, 4 × 106 transfected HCT116 cells or 8 × 106 transfected SW480 cells in 0.1 mL PBS were injected into mice subcutaneously, and these mice were randomly divided into the experimental and control group."
item2194 "Briefly, 4 × 106 transfected HCT116 cells or 8 × 106 transfected SW480 cells in 0.1 mL PBS were injected into mice subcutaneously, and these mice were randomly divided into the experimental and control group."
item2195 "A375 cells (1 × 107) transfected with sh-METTL3 or shNC lentiviruses were subcutaneously injected into the right flank of the mice. After 33 days, the mice were sacrificed and the xenografted tumors were collected for immunohistochemistry analysis. Animal experiments were approved by the Ethics Committee of Shanghai East Hospital (K-KYSB-2020-0)."
item2196 .
item2197 .
item2198 .
item2199 .
item2200 .
item2201 .
item2202 "The male C57BL/6 mice (SPF, Eight-week-old) were randomly divided into four groups: sham (n = 6), model (n = 6), model + adeno-associated virus-negative control (AVV-shNC) (n = 6), and model + AVV-shWTAP groups (n = 6). After acclimating for one week, mice in other groups were treated with DMM surgery to induce OA as previously described except for the sham groups [19]. The mice in sham group were underwent only the skin of the right knee joint incision. The AAV-shNC and AAV-shWTAP were constructed by HANBIO (Shanghai, China). The AAV-shNC and AAV-shWTAP groups were intra-articularly injected with 10 μL of AAV-shNC and AAV-shWTAP (1 × 1013 vg/ml) through the medial parapatellar area at two weeks after the DMM operation, respectively."
item2203 "The male C57BL/6 mice (SPF, Eight-week-old) were randomly divided into four groups: sham (n = 6), model (n = 6), model + adeno-associated virus-negative control (AVV-shNC) (n = 6), and model + AVV-shWTAP groups (n = 6). After acclimating for one week, mice in other groups were treated with DMM surgery to induce OA as previously described except for the sham groups [19]. The mice in sham group were underwent only the skin of the right knee joint incision. The AAV-shNC and AAV-shWTAP were constructed by HANBIO (Shanghai, China). The AAV-shNC and AAV-shWTAP groups were intra-articularly injected with 10 μL of AAV-shNC and AAV-shWTAP (1 × 1014 vg/ml) through the medial parapatellar area at two weeks after the DMM operation, respectively."
item2204 "The male C57BL/6 mice (SPF, Eight-week-old) were randomly divided into four groups: sham (n = 6), model (n = 6), model + adeno-associated virus-negative control (AVV-shNC) (n = 6), and model + AVV-shWTAP groups (n = 6). After acclimating for one week, mice in other groups were treated with DMM surgery to induce OA as previously described except for the sham groups [19]. The mice in sham group were underwent only the skin of the right knee joint incision. The AAV-shNC and AAV-shWTAP were constructed by HANBIO (Shanghai, China). The AAV-shNC and AAV-shWTAP groups were intra-articularly injected with 10 μL of AAV-shNC and AAV-shWTAP (1 × 1015 vg/ml) through the medial parapatellar area at two weeks after the DMM operation, respectively."
item2205 .
item2206 "Six-week-old BALB/c-nu mice (n = 10) were purchased from Hunan STA Laboratory Animal Co., Ltd. Nude mice were adaptively fed in a specific pathogen free (SPF) environment for 7 days. The study protocol was ethically approved by the Kunming Yan'an Hospital Experimental Animal Ethics Committee (Kunming, China; approval no. 2020004). Mice were randomly divided into a control group (HepG2) and an experimental group (HepG2/Sora) with 5 mice in each group. A cell suspension (4 × 106 cells per mouse) was injected into the right lateral thighs of mice after light anesthesia using 37.5 mg/kg pelltobarbitalum natricum (cat.no. P-010; Sigma-Aldrich LLC.). The drug treatment was carried out when the tumor size was approximately 100 mm3. Sorafenib was prepared with 0.4% DMSO+PBS solution and administered to mice by intraperitoneal injection at a dose of 100 mg/kg after light anesthesia using 37.5 mg/kg pelltobarbitalum natricum. Sorafenib was administered once a day for 5 consecutive days. The physical state of the nude mice was observed and recorded every day. Mice in poor condition were terminated in time and euthanized immediately. All the mice were sacrificed using intraperitoneal injection of 200 mg/kg pelltobarbitalum natricum 5 days after sorafenib administration. Before euthanasia, the mice were given oral administration of ibuprofen (40 mg/kg; cat.no.14883; Sigma-Aldrich LLC.) with water to relieve pain. The tumors were removed surgically and photographed using a camera."
item2207 .
item2208 "Thirty female BALB/c nude mice weighing 18-22 g were randomly assigned to six groups. PC9-Mock, PC9-circFUT8, A549-sh-scramble, and A549-sh-circFUT8 cells were prepared as a suspension of 4 × 105 cells in 200 μL saline, respectively, and injected into the tail vein. Mice were sacrificed at 6 weeks post injection and examined microscopically by hematoxylin and eosin (H&E) staining for the development of lung metastases."
item2209 "Male 5-week-old BALB/c-nu mice were provided by Shanghai SLAC Laboratory Animal CO. LTD. Cells (5 × 106 cells suspended in 0.1 mL PBS) were injected subcutaneously from the axilla of each nude mouse. After 12 days, the long (L) and short (S) diameters of the tumors were measured with vernier caliper every 3 days (0.5 × L × S2). The growth curve of subcutaneous tumors was drawn on the basis of the measured tumor volume. The experimental subjects were killed 33 days after the injection of cells, and subcutaneous tumors were removed completely."
item2210 "Sixteen SPF female BALB/c nude mice (6 weeks old, weighing 20-22 g) (SLAC Laboratory Animal, Shanghai, China) were randomly arranged into the sh-NC group and the sh-METTL3 group, with 8 mice per group. After transfected with sh-NC and sh-METTL3, respectively, cells were rinsed with PBS to remove excess medium, detached with 0.25% trypsin, and detachment was terminated with complete medium, followed by centrifugation to collect cell precipitates. Appropriate amounts of physiological saline were added and gently pipetted into single-cell suspensions for cell counting. A total of 3 × 106 cells were resuspended in 50 μL normal saline, mixed with 50 μL 25% Matrigel (1:1), and inoculated subcutaneously into the right inguinal of nude mice. Tumor growth curves were plotted with 7, 14, 21, and 28 days as time points, and the short diameter (a) and long diameter (b) of the tumor were determined using a vernier caliper to estimate the tumor volume V = (a2b)/2. Tumor mass was weighed on a scale. Following 4 weeks, nude mice were euthanized by an intraperitoneal injection of 150 mg/kg pentobarbital (P3761, Sigma). The skin near the tumor was cut short using surgical scissors, and the tumor was separated using ball pointed pliers. The tumors were partially embedded with paraffin for histological examination, and the remaining part was kept at -80°C."
item2211 .
item2212 "Cells (si-NC group, si-YTHDF1 group, si-TRIM68 group, si-YTHDF1 + OE-TRIM68 group) were digested with 0.25% trypsin, and fetal bovine serum was added to prevent excessive digestion. After centrifugation at 1000 rpm for 5 min, cells were collected and suspended with culture medium without serum, and cell density was adjusted to 5 × 107 cells/ml. SPF male BALB/c-nu nude mice (n = 20) were used in this study. Tumor cells (100 μl) were inoculated subcutaneously in the axils of the right forelimbs of mice. After the injection, the mental state, activity, diet, urine and feces of the nude mice were observed regularly every day. The body weight was measured weekly with an electronic balance, and the diameters of subcutaneous graft were measured weekly with vernier caliper. After the tumor grew to about 40 mm3, the nude mice were given flutamine (100 mg/kg; continuous administration for 1 week). The subcutaneous graft tumor volume V (mm3) = longest tumor diameter (mm) × shortest tumor diameter (mm) × 0.5. According to the tumor volume obtained, the growth curve of transplanted tumor was plotted. Five weeks later, the mice were weighed, and sacrificed by cervical dislocation method. The tumor was separated and rinsed with sterile PBS, and then weighed. After weighing, whole blood was taken to separate serum, and tumor tissue was taken. One part was fixed in 4% paraformaldehyde solution, and the other part was stored at -80 °C."
item2213 .
item2214 .
item2215 "An anterior cruciate ligament transection (ACLT)-induced OA model was constructed in mice. Briefly, 10-week-old male C57BL/6 mice were anesthetized, and the skin was prepared. The knee joint capsule was opened, and the ACL was carefully transected with microsurgical scissors under a microscope. For the sham operation, the right knee joints were exposed, but the ligament was not transected. At 1 week, 3 weeks, 5 weeks and 7 weeks after the operation, 10 μl of DMEM containing 1 * 10^5 control MSCs or ALKBH5-overexpressing MSCs was injected into the knee joint with an insulin syringe (BD Bioscience, 324702), with 10 μl of DMEM as a control."
item2216 "An anterior cruciate ligament transection (ACLT)-induced OA model was constructed in mice. Briefly, 10-week-old male C57BL/6 mice were anesthetized, and the skin was prepared. The knee joint capsule was opened, and the ACL was carefully transected with microsurgical scissors under a microscope. For the sham operation, the right knee joints were exposed, but the ligament was not transected. At 1 week, 3 weeks, 5 weeks and 7 weeks after the operation, 10 μl of DMEM containing 1 * 10^5 control MSCs or ALKBH5-overexpressing MSCs was injected into the knee joint with an insulin syringe (BD Bioscience, 324702), with 10 μl of DMEM as a control."
item2217 "Three- to four-week-old female BALB/c nude mice were obtained from Guangxi Medical University Laboratory Animal Center and maintained under SPF housing. NPC cells were resuspended in 100 μl of PBS and injected subcutaneously into the flanks of the nude mice. Mice were randomized to experimental groups 28 days after implantation and were injected intraperitoneally with the same volume of FB23-2 (2 mg/kg) or erastin (10 mg/kg) or vehicle every three days during radiation (4 Gy, once a week). The endpoint of all experiments was tumor size."
item2218 "Three- to four-week-old female BALB/c nude mice were obtained from Guangxi Medical University Laboratory Animal Center and maintained under SPF housing. NPC cells were resuspended in 100 μl of PBS and injected subcutaneously into the flanks of the nude mice. Mice were randomized to experimental groups 28 days after implantation and were injected intraperitoneally with the same volume of FB23-2 (2 mg/kg) or erastin (10 mg/kg) or vehicle every three days during radiation (4 Gy, once a week). The endpoint of all experiments was tumor size."
item2219 "B6, B6/SJL (CD45.1+ congenic) and Rag2-/-Il2rg-/- mice were from Taconic. Mettl3fl/fl mice were generated by J. H. Hanna at Weizmann Institute of Science. Klrg1Cre mice were generated and provided by R. Flavell at Yale University. Rorc-egfp reporter mice were provided by I. Ivanov at Columbia University. Rosa26CreERT2 mice were from Jackson Laboratories. To induce Cre expression in Mettl3-/- mice, the recipients were given 2 μg of tamoxifen in 100 μl of corn oil i.p. every other day for three doses. Mice used for experiments were females between 6 and 18 weeks of age, unless otherwise specifically indicated."
item2220 .
item2221 .
item2222 .
item2223 .
item2224 "For tumor growth measurement, 5 × 106 stably transfected 22RV1 cells were subcutaneously injected into mice at the flank site. Then, the volume (V) of xenograft tumors was examined once per week as follows: V = length × width2/2. After 4 weeks, the mice were euthanized via intraperitoneal injection of 150 mg/kg pentobarbital sodium, and the tumors were taken out and weighed, and collected for immunohistochemistry (IHC) to examine the expression of the proliferation marker Ki67 (1:200, ab16667, Abcam) in xenograft tumors. For tumor metastasis measurement, 5 × 106 stably transfected 22RV1 cells were injected into mice through tail vein."
item2225 "For tumor growth measurement, 5 × 106 stably transfected 22RV1 cells were subcutaneously injected into mice at the flank site. Then, the volume (V) of xenograft tumors was examined once per week as follows: V = length × width2/2. After 4 weeks, the mice were euthanized via intraperitoneal injection of 150 mg/kg pentobarbital sodium, and the tumors were taken out and weighed, and collected for immunohistochemistry (IHC) to examine the expression of the proliferation marker Ki67 (1:200, ab16667, Abcam) in xenograft tumors. For tumor metastasis measurement, 5 × 106 stably transfected 22RV1 cells were injected into mice through tail vein."
item2226 "A total of 1 × 106 HuCCT1-sgNC and HuCCT1-sgMETTL5 cells in 0.2 mL of PBS were separately injected into 6-week-old male NCG mice (N = 7 per group) via caudal vein. Mice were sacrificed at 4 weeks after injection, and the lung tissues were processed into 4-mm-thick paraffin-embedded sections. H&E staining was subsequently performed to determine the pulmonary metastasis."
item2227 .
item2228 .
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item2230 .
item2231 .
item2232 .
item2233 .
item2234 .
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item2238 .
item2239 "For ICH model establishment, the mice in the ICH group were intraperitoneally injected with 2% sodium pentobarbital (0.2 mL/100 g body weight). Subsequently, the mice were routinely sterilized, skin prepared and fixed on a brain stereotaxic apparatus. Then 0.2 units of collagenase 4 were injected into the left caudate putamen (0.8 mm posterior to the bregma, 3.5 mm lateral to the left, and 5.5 mm deep). The sham-operated control group was injected with an equal volume of normal saline. For METTL3 knockdown, the mice were intravenously injected with sh-METTL3 or sh-NC (1×109 plaque forming unit)."
item2240 .
item2241 "For the action of CDCA4, mice were set into Control, sh-NC, sh-CDCA4 groups, 6 mice/group. For the action of ALKBH5 in CDCA4, mice were set into oe-NC, oe-CDCA4, oe-CDCA4 + sh-NC, oe-CDCA4 + sh-ALKBH5 groups, 6 mice/group. In brief, 1 × 106/100 μL LLC cells were injected subcutaneously into the right flank of mice to establish a tumor model. LLC cells stably transfected with oe-NC, oe-CDCA4, oe-CDCA4 + sh-NC and oe-CDCA4 + sh-ALKBH5 were injected into each group of mice ."
item2242 "For the action of CDCA4, mice were set into Control, sh-NC, sh-CDCA4 groups, 6 mice/group. For the action of ALKBH5 in CDCA4, mice were set into oe-NC, oe-CDCA4, oe-CDCA4 + sh-NC, oe-CDCA4 + sh-ALKBH5 groups, 6 mice/group. In brief, 1 × 106/100 μL LLC cells were injected subcutaneously into the right flank of mice to establish a tumor model. LLC cells stably transfected with oe-NC, oe-CDCA4, oe-CDCA4 + sh-NC and oe-CDCA4 + sh-ALKBH5 were injected into each group of mice ."
item2243 "Ten BALB/c nude mice (21 days) were randomly divided into two groups (NC group and si-CACNA1G-AS1 group). In the NC group, we performed subcutaneous injection with 0.2 ml SKOV3 cells (2 × 107/ml) on the back of each mouse, while in the si-CACNA1G-AS1 group, we chose 0.2 ml CACNA1G-AS1 knockout SKOV3 cells (2 × 107/ml) for injection. During this process, we set the puncture point 1.0 cm from the injection site. Each mouse was injected once, and the next day was marked as the first day after inoculation. Meanwhile, 1 ml PBS buffer supplemented with ferric ammonium citrate (FAC, with 100 μmol/L Fe2+) was injected alongside the tumor borders at 0, 6, 12, 18 and 24 days after subcutaneous inoculation of ovarian cancer cells. We chose 6, 12, 18, 24, 30, 36, 42 and 48 days post tumor cell injection as assessment points, where we measured the long/short diameter of tumors with calipers and then calculated tumor volume according to the formula: length × width2/2. All tumor-bearing mice were euthanized by intraperitoneal injection with pentobarbital sodium (150-200 mg/kg) on the 50th day, and then these subcutaneous transplant tumors were removed, weighed and analyzed."
item2244 "MM1S cells infected with sh-METTL3/sh-NC (selected by 2 μg/mL puromycin) were subcutaneously injected into the left abdominal flanks of NOD-SCID mice (SPF Biotechnology Co., Ltd., Beijing, China) (n = 6/group). Tumor volume was measured every 7 days using the calipers. After 28 days, the mice were sacrificed, and tumor tissues were collected, weighed, and photographed. One part of the tissue was used to detect the expression of METTL3 and BZW2 using qRT-PCR and WB analysis, and the other part was prepared into paraffin sections and then performing Ki-67 immumohistochemical (IHC) staining according to the SP kit (Solarbio, Beijing, China) instructions."
item2245 "Male BALB/c nude mice (18-20 g weight, 6-week-old) were acquired from Beijing Vital River Laboratory Animal Technology Co., Ltd. (China). Xenograft tumors were produced through subcutaneously injecting 2×105 control or sh-LRPPRC-transfected HepG2 cells under the arm. Tumor growth was measured by use of slide caliper every 3 days. Tumor volume was computed utilizing the formula 1/2 × (length × width2)."
item2246 .
item2247 .
item2248 "GDM group and control group. GDM group was fed with high-fat diet (Research Diets D12451) for 1 week to mating and throughout pregnancy, while control group was fed with normal diet (Research Diet 1022). Then the two groups were mated with age-matched male mice. The presence of a vaginal plug was considered gestational day (GD) 0.5. Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed at GD0.5, GD11.5, and GD16.5. Six mice of each group were killed at GD18.5 to collect the liver tissue of the fetus. The remaining mice were left to delivery. All female offspring were selected for this study. Fetus weaning was done and then was separately fed with a standard diet for 12 weeks. OGTT and ITT were performed at 12 weeks. Then F1 generation was killed to collect serum and liver tissue."
item2249 "Male BALB/c nude mice that aged 4 to 6-week-old were bought from Vital River Laboratory (China). SW480 cells transfected with control or METTL16 overexpression vectors (5 x 106 cells/site) were suspended in 50 μl saline and inoculated into the fact pat on back. Tumor size was measured and calculated every three days. After feeding for 30 days, the tumors were collected and made into paraffine-embedded samples."
item2250 "After anesthetization with an intraperitoneal injection of ketamine (80 mg/kg) and xylazine (10 mg/kg), none of the rats had obvious abdominal distention, ascites, discomfort, or pain. Laminectomy was performed at T8-T10 (counting from top to bottom; from the 8th thoracic vertebra to the 10th thoracic vertebra). The spine was immobilized with a stereotaxic device, and the exposed spinal cord was severely injured at the center between T8 and T10 by a 5-g weight dropping freely from a height of 8 cm (Perot et al., 1987; Ray et al., 2000). After surgery, the rats were carefully nursed and fed and their urine was squeezed three times a day until reflex bladder emptying was established. Successful modeling was defined as (1) rapid retraction of the whole body, (2) rapid edema and congestion on the surface of the local spinal cord, (3) intact spinal dura mater, (4) flaccid paralysis of the hindlimbs, and (5) survival. Rats that underwent unsuccessful modeling were replaced. The SCI (1 d), SCI (7 d), and SCI (14 d) groups were killed on first, seventh, and 14th day after modeling, respectively, while the other groups were killed on the 14th day. Most operations in the sham group were the same as those in the SCI group, with no spinal cord contusions. The SCI + LV-NC, SCI + LV-UBR1, SCI + sh-NC, and SCI + sh-METTL14 groups were given lentiviral tail vein injections (once every 3 d, virus titers of 108 TU/ml; GenePharma) 3 d before SCI modeling."
item2251 "In vivo tumor growth assay, 1 × 107 Hep3B-GBAP1 or MHCC97H-shGBAP1 subclones and the corresponding control cells were transplanted into the body of 6-week-old BALB/c nude mice (Slac Laboratory Animal Center, Shanghai, China) via subcutaneous injection. Tumor size was measured every 3 days. Twenty-one days later, tumors were removed from mice in different groups. Tumor weight was calculated after dissection. Permission of conducting animal study was obtained from the Research Ethics Committee of Xi'an Jiaotong University. In vivo lung metastasis model, 1 × 106 cells were intravenously injected into the lateral tail vein of nude mice (n = 5 mice/group)."
item2252 "In vivo tumor growth assay, 1 × 107 Hep3B-GBAP1 or MHCC97H-shGBAP1 subclones and the corresponding control cells were transplanted into the body of 6-week-old BALB/c nude mice (Slac Laboratory Animal Center, Shanghai, China) via subcutaneous injection. Tumor size was measured every 3 days. Twenty-one days later, tumors were removed from mice in different groups. Tumor weight was calculated after dissection. Permission of conducting animal study was obtained from the Research Ethics Committee of Xi'an Jiaotong University. In vivo lung metastasis model, 1 × 106 cells were intravenously injected into the lateral tail vein of nude mice (n = 5 mice/group)."
item2253 "Eight-week-old male ApoE-/- and C57BL/6J mice were purchased from GemPharmatech Co., Ltd. All mice were housed in a 12-h light-dark environment with free access to food and water. ApoE-/- mice were fed a high-fat diet (21% crude fat, 0.15% cholesterol, 19.5% casein) for 8 weeks to establish an animal model of AS. C57BL/6J mice in control group were kept on a normal diet. Next, the modeled animals were randomly divided into 3 groups (n = 10): (1) mice in the AS group were fed with HFD for 8 weeks; (2) mice in the AS + sh-NC group were given Ad-sh-NC (2 × 109 pfu/mouse) injection every 14 days through the tail vein for 8 weeks, during which HFD was used to feed mice; (3) mice in the AS + sh-NPC1L1 group were given Ad-sh-NPC1L1 (2 × 109 pfu/mouse) injection every 14 days through the tail vein for 8 weeks, during which HFD was used to feed mice."
item2254 .
item2255 .
item2256 "For the tumor growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-VIRMA were injected subcutaneously into the axilla of mice, and the tumor size was measured every 4 days. After 32 days, the mice were sacrificed, and the tumors were retrieved. For the tumor inguinal lymph node metastasis model, 1 × 106 scrambled or sh-VIRMA SUNE-1 cells were injected into the footpads of mice. After 6 weeks, the mice were euthanized. The footpad tumors and inguinal lymph nodes were excised. Tumors and lymph nodes were subjected to subsequent in situ hybridization and immunohistochemistry analysis."
item2257 "For the tumor growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-VIRMA were injected subcutaneously into the axilla of mice, and the tumor size was measured every 4 days. After 32 days, the mice were sacrificed, and the tumors were retrieved. For the tumor inguinal lymph node metastasis model, 1 × 106 scrambled or sh-VIRMA SUNE-1 cells were injected into the footpads of mice. After 6 weeks, the mice were euthanized. The footpad tumors and inguinal lymph nodes were excised. Tumors and lymph nodes were subjected to subsequent in situ hybridization and immunohistochemistry analysis."
item2258 "To evaluate the tumorigenic effect of FTO, FTO knockdown or control AGS B95.8 cells (1 × 107 suspended in 150 μL of PBS) were subcutaneously injected into the flanks of B-NDG mice. The diameter and width of the tumours were measured every 4 days and used to estimate the tumour volumes by the standard formula: 0.5 × length × width2. At the end stage, the tumours were removed, imaged and weighed. To investigate the peritoneal dissemination ability of EBVaGC cells, intraperitoneal injection was performed. Briefly, 1 × 107 FTO silencing or control EBVaGC cells suspended in 0.4 mL of PBS were injected into the peritoneal cavity of each mouse. Eight weeks later, all the mice were sacrificed, and the abdominal and intestinal metastatic nodules were excised, counted, photographed and paraffin embedded. For the lung metastasis model, 200 μL of 1 × 106 luciferase-labelled EBVaGC cells from different groups were directly injected into the tail vein of B-NDG mice, and distant and lung metastasis were evaluated using bioluminescent imaging. After 6 or 8 weeks, the mice were euthanized, and the lungs were embedded in paraffin and subjected to haematoxylin and eosin (H&E) staining to record the micrometastatic nodules using a microscope. All animal experiments were approved by our Institutional Animal Care."
item2259 "To evaluate the tumorigenic effect of FTO, FTO knockdown or control AGS B95.8 cells (1 × 107 suspended in 150 μL of PBS) were subcutaneously injected into the flanks of B-NDG mice. The diameter and width of the tumours were measured every 4 days and used to estimate the tumour volumes by the standard formula: 0.5 × length × width2. At the end stage, the tumours were removed, imaged and weighed. To investigate the peritoneal dissemination ability of EBVaGC cells, intraperitoneal injection was performed. Briefly, 1 × 107 FTO silencing or control EBVaGC cells suspended in 0.4 mL of PBS were injected into the peritoneal cavity of each mouse. Eight weeks later, all the mice were sacrificed, and the abdominal and intestinal metastatic nodules were excised, counted, photographed and paraffin embedded. For the lung metastasis model, 200 μL of 1 × 106 luciferase-labelled EBVaGC cells from different groups were directly injected into the tail vein of B-NDG mice, and distant and lung metastasis were evaluated using bioluminescent imaging. After 6 or 8 weeks, the mice were euthanized, and the lungs were embedded in paraffin and subjected to haematoxylin and eosin (H&E) staining to record the micrometastatic nodules using a microscope. All animal experiments were approved by our Institutional Animal Care."
item2260 "To evaluate the tumorigenic effect of FTO, FTO knockdown or control AGS B95.8 cells (1 × 107 suspended in 150 μL of PBS) were subcutaneously injected into the flanks of B-NDG mice. The diameter and width of the tumours were measured every 4 days and used to estimate the tumour volumes by the standard formula: 0.5 × length × width2. At the end stage, the tumours were removed, imaged and weighed. To investigate the peritoneal dissemination ability of EBVaGC cells, intraperitoneal injection was performed. Briefly, 1 × 107 FTO silencing or control EBVaGC cells suspended in 0.4 mL of PBS were injected into the peritoneal cavity of each mouse. Eight weeks later, all the mice were sacrificed, and the abdominal and intestinal metastatic nodules were excised, counted, photographed and paraffin embedded. For the lung metastasis model, 200 μL of 1 × 106 luciferase-labelled EBVaGC cells from different groups were directly injected into the tail vein of B-NDG mice, and distant and lung metastasis were evaluated using bioluminescent imaging. After 6 or 8 weeks, the mice were euthanized, and the lungs were embedded in paraffin and subjected to haematoxylin and eosin (H&E) staining to record the micrometastatic nodules using a microscope. All animal experiments were approved by our Institutional Animal Care."
item2261 .
item2262 .
item2263 "Briefly, the Mettl3 targeting vector was designed to flank exon 2-3 with LoxP sites and electroporated into C57BL/6 embryonic stem cells (ES). Positive ES clones were confirmed by PCR and sequencing, and injected into C57BL/6 blastocysts to generate chimeric offspring. Chimeric mice were mated with C57BL/6 mice to obtain Mettl3flox/flox mice. These Mettl3flox/flox mice were crossed with Lyz2-Cre mice (The Jackson Laboratory) to generate Mettl3fl/fl (WT) and Mettl3fl/flLyz2Cre/+ (KO) mice."
item2264 "Briefly, the Mettl3 targeting vector was designed to flank exon 2-3 with LoxP sites and electroporated into C57BL/6 embryonic stem cells (ES). Positive ES clones were confirmed by PCR and sequencing, and injected into C57BL/6 blastocysts to generate chimeric offspring. Chimeric mice were mated with C57BL/6 mice to obtain Mettl3flox/flox mice. These Mettl3flox/flox mice were crossed with Lyz2-Cre mice (The Jackson Laboratory) to generate Mettl3fl/fl (WT) and Mettl3fl/flLyz2Cre/+ (KO) mice."
item2265 "Intracranial xenografts were generated by implanting 20,000 patient derived GSCs into the right cerebral cortex of mice at a depth of 3.5 mm. Housing conditions and animal status were supervised by a veterinarian. Euthanasia was taken until neurologic symptoms included hunched posture, gait changes, or lethargy were observed, at which point they were sacrificed. Brains were harvested and frozen at -80 °C with O.C.T. compound (4583, Tissue-Tek) directly or fixed in 4 % formaldehyde for 48 hours then stored in 70 % ethanol, and sectioned."
item2266 "Intracranial xenografts were generated by implanting 20,000 patient derived GSCs into the right cerebral cortex of mice at a depth of 3.5 mm. Housing conditions and animal status were supervised by a veterinarian. Euthanasia was taken until neurologic symptoms included hunched posture, gait changes, or lethargy were observed, at which point they were sacrificed. Brains were harvested and frozen at -80 °C with O.C.T. compound (4583, Tissue-Tek) directly or fixed in 4 % formaldehyde for 48 hours then stored in 70 % ethanol, and sectioned."
item2267 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
item2268 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
item2269 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
item2270 .
item2271 .
item2272 "A total of 16 male, 4- to 6-week-old BALB/c nude mice weighing 18-26 g, were purchased from the Institute of Model Animals, Nanjing University and raised in a specific pathogen-free (SPF) environment for the xenograft model. The mice were raised in animal individually ventilated cage (IVC cages) with a room temperature of 24°C and relative humidity of 70% and the air exchange rate was 15 times/h. The mice could drink filtered tap water and commercial feed ad libitum under a strict 12-h light/dark cycle. The animal laboratory was cleaned twice one day and sterilized with ultraviolet light for 1 h each week. Each mouse was injected with 100 μl PBS containing 1×106 T24 cell lines expressing either LV-NC or LV-shMETTL3 subcutaneously under the right armpit. Tumor volumes were recorded on day 7 after the injection and then measured each week. At day 28, the mice were sacrificed by cervical dislocation following the inhalation of 3% isoflurane anesthesia and the death of the mice was confirmed by respiratory arrest. Tumor weights were also compared. The data from each group of mice are expressed as the mean ± standard deviation (SD)."
item2273 "A total of 16 male, 4- to 6-week-old BALB/c nude mice weighing 18-26 g, were purchased from the Institute of Model Animals, Nanjing University and raised in a specific pathogen-free (SPF) environment for the xenograft model. The mice were raised in animal individually ventilated cage (IVC cages) with a room temperature of 24°C and relative humidity of 70% and the air exchange rate was 15 times/h. The mice could drink filtered tap water and commercial feed ad libitum under a strict 12-h light/dark cycle. The animal laboratory was cleaned twice one day and sterilized with ultraviolet light for 1 h each week. Each mouse was injected with 100 μl PBS containing 1×106 T24 cell lines expressing either LV-NC or LV-shMETTL3 subcutaneously under the right armpit. Tumor volumes were recorded on day 7 after the injection and then measured each week. At day 28, the mice were sacrificed by cervical dislocation following the inhalation of 3% isoflurane anesthesia and the death of the mice was confirmed by respiratory arrest. Tumor weights were also compared. The data from each group of mice are expressed as the mean ± standard deviation (SD)."
item2274 "For tumor growth under ionizing radiation (IR), 6-week- old NSG male mice were injected with 5 × 106 cancer cells infected with lentivirus or shRNAs and/or expression vectors in 100 μl PBS with 100 μl of Matrigel matrix (BD Bioscience) in one side of flanks. After injection of tumor cells into mice, right flank tumors were radiated by 10 Gy X-ray beam for 14 days and monitored until they reached maximum tumor volumes of 1,000 mm3. Subsequently, tumor growth was measured with a caliper every 7 days."
item2275 "Six-week-old male BALB/c nude mice were randomly assigned to three groups (n = 6 in each group): Lv-sh-NC, Lv-sh-IGF2BP2#1, and Lv-sh-IGF2BP2#2. Mice in each group received the subcutaneous injection of 1 × 106 cells (0.1 mL) into the upper right flanks of mice. Cells were pre-transduced with sh-NC, sh-IGF2BP2#1, or sh-IGF2BP2#2 for 48 h before the injection. Starting from day 10 of the injection, the tumor volume was measured every 3 days. Mice were anesthetized and euthanized at day 25 of the injection, tumors were removed and collected, tumor weight was determined in a blind manner."
item2276 "For the tumor growth study, nude mice were subcutaneously inoculated into the flanks with PC cells (3 × 106) suspended in 0.1 mL PBS. Tumor volumes were calculated as 1/2 (length × width2) weekly. After 4 weeks, the xenograft tumors were harvested for analysis. To examine the role of the FAK inhibitor in tumor growth, nude mice were inoculated subcutaneously into the flanks with 3 × 106 stable cells suspended in 0.1 mL PBS. After 1 week, pre-established tumor xenografts were treated with Y15 (0.5 mg/kg, 2 ×/week × 3) (MCE, New Jersey, China). After another 3 weeks, the xenograft tumors were harvested for analysis. In the metastasis model, PC cells (2 × 106) in 0.1 mL PBS were injected into nude mice through their tail veins. Six weeks later, the lungs were removed for the evaluation of lung metastasis using hematoxylin and eosin (H&E) staining."
item2277 "For the tumor growth study, nude mice were subcutaneously inoculated into the flanks with PC cells (3 × 106) suspended in 0.1 mL PBS. Tumor volumes were calculated as 1/2 (length × width2) weekly. After 4 weeks, the xenograft tumors were harvested for analysis. To examine the role of the FAK inhibitor in tumor growth, nude mice were inoculated subcutaneously into the flanks with 3 × 106 stable cells suspended in 0.1 mL PBS. After 1 week, pre-established tumor xenografts were treated with Y15 (0.5 mg/kg, 2 ×/week × 3) (MCE, New Jersey, China). After another 3 weeks, the xenograft tumors were harvested for analysis. In the metastasis model, PC cells (2 × 106) in 0.1 mL PBS were injected into nude mice through their tail veins. Six weeks later, the lungs were removed for the evaluation of lung metastasis using hematoxylin and eosin (H&E) staining."
item2278 .
item2279 .
item2280 "Briefly, mice were anesthetized, and the right common carotid artery (CCA) and external and internal carotid arteries were surgically exposed through a neck incision. Then, a filament was introduced into the CCA and advanced into the internal carotid artery until the tip reached the origin of the middle cerebral artery (MCA). Occlusion of the right MCA was induced either transiently for 60 min followed by a reperfusion period of different hours. Sham-operated mice received the same experimental surgery without a filament being inserted into the MCA. For experiments, mice were randomly divided into Sham groups (n = 6), MCAO/R-1 day (n = 6), MCAO/R-3 day (n = 6), and MCAO/R-5 day (n = 6). To investigate the effect of m6A modification on the MCAO/R injury in vivo, thirty-six mice were divided into four groups, including Sham group (n = 12), DMSO group (n = 12), Cycloleucine (Cyc) group (n = 6), and S-Adenosyl-L-homocysteine (SAH) group (n = 6). Cyc, a specific inhibitor of S-adenosyl-methionine mediated methylation (50 mM/kg, MedChemExpress), and SAH (50 mg/kg, Selleck), another m6A specific inhibitor which specifically inhibits the METTL3-METTL14 heterodimer complex, or DMSO (as control) were intraperitoneally injected into the mice for consecutive 3 days before MCAO injury. Three days after MCAO/R injury, the mice brains were collected for the following experiments."
item2281 .
item2282 .
item2283 "For xenograft studies, female mice between 4 and 6 weeks of age were used. Monoclonal cell lines showing a nearly complete absence of Ythdf2 and Ddx58 were used for in vivo experimentation. Mice were implanted with 2 × 106 MGHU3 cells resuspended in 100 μL of Matrigel Matrix High Concentration (354248) and PBS mixture subcutaneously into the right flanks. Subcutaneous tumor growth was measured weekly by calipers, and the volume was calculated using the formula: volume = (length × width2)/2."
item2284 .
item2285 .
item2286 .
item2287 .
item2288 "Male C57BL/6 mice were intraperitoneally injected with MPTP hydrochloride (30 mg/kg/day, Sigma, USA) for 5 consecutive days. Control mice were injected with equal volume of 0.9% saline. 3 days after the last injection of MPTP, behavioral tests were performed."
item2289 .
item2290 "For tumor growth assay, 2 × 106 OS cells in 100 μL medium were injected into the nude mice subcutaneously. For tumor metastasis assay, we injected medium containing 2 × 106 cells through the caudal vein. An IVIS200 imaging system (Caliper Life Science, USA) was used to image and assess the OS metastasis. For pharmacological inhibition of USP13, mice bearing xenografts were treated with Spautin-1 (40 mg/kg/day i.p.) or vehicle for 2 weeks."
item2291 "For tumor growth assay, 2 × 106 OS cells in 100 μL medium were injected into the nude mice subcutaneously. For tumor metastasis assay, we injected medium containing 2 × 106 cells through the caudal vein. An IVIS200 imaging system (Caliper Life Science, USA) was used to image and assess the OS metastasis. For pharmacological inhibition of USP13, mice bearing xenografts were treated with Spautin-1 (40 mg/kg/day i.p.) or vehicle for 2 weeks."
item2292 .
item2293 .
item2294 .
item2295 "Col1alpha2creER mice were crossed with ALKBH5flox/flox mice (Cyagen, China) to generate mice heterozygous for both alleles. Then ALKBH5flox/flox mice and heterozygous mice from the first cross produced ALKBH5flox/floxCol1alpha2creER mice, which were used in experiments. The 6-week-old ALKBH5flox/floxCol1alpha2creER mice were administration intraperitoneal injection of the tamoxifen suspension (30 mg/kg, Sigma-Aldrich) for 7 days. The ALKBH5flox/flox mice and Col1alpha2creER mice as control were injected in in the same manner."
item2296 "Col1alpha2creER mice were crossed with ALKBH5flox/flox mice (Cyagen, China) to generate mice heterozygous for both alleles. Then ALKBH5flox/flox mice and heterozygous mice from the first cross produced ALKBH5flox/floxCol1alpha2creER mice, which were used in experiments. The 6-week-old ALKBH5flox/floxCol1alpha2creER mice were administration intraperitoneal injection of the tamoxifen suspension (30 mg/kg, Sigma-Aldrich) for 7 days. The ALKBH5flox/flox mice and Col1alpha2creER mice as control were injected in in the same manner."
item2297 .
item2298 .
item2299 "Thirty-six specific pathogen-free male BALB/c-nude mice (age, 5-6 weeks) were randomly assigned to the groups: CAL33/shMETTL14#2, CAL33/shMETTL14#3, CAL33/shNC and HSC3/shMETTL14#2, HSC3/shMETTL14#3, HSC3/shNC (n = 6 per group). Fifty microliters of PBS buffer containing approximately 1 × 106 cells was injected into the left tongue under 2% pentobarbital sodium intraperitoneal injection anesthesia to establish a tumor xenograft. The weight of the mice was measured every 3 days after one week until they lost more than 15% of their body weight in a short period of time."
item2300 "Thirty-six specific pathogen-free male BALB/c-nude mice (age, 5-6 weeks) were randomly assigned to the groups: CAL33/shMETTL14#2, CAL33/shMETTL14#3, CAL33/shNC and HSC3/shMETTL14#2, HSC3/shMETTL14#3, HSC3/shNC (n = 6 per group). Fifty microliters of PBS buffer containing approximately 1 × 106 cells was injected into the left tongue under 2% pentobarbital sodium intraperitoneal injection anesthesia to establish a tumor xenograft. The weight of the mice was measured every 3 days after one week until they lost more than 15% of their body weight in a short period of time."
item2301 "Male BALB/c-nude mice (5-week-old) were used in this study. First, the Antigo miR-1908-5p (Antigo-miR) for knocking down miR-1908-5p as well as its negative control (Antigo-NC) were acquired from GenePharma (China). Antigo-miR and Antigo-NC were each introduced into C666-1 cells which were then delivered through the tail of every mouse (n = 3 per group). Thereafter, all mice were raised well, and the long diameter and short diameter of tumors were measured every week. The tumor volume was calculated as tumor volume (mm3) = (short diameter)2 × long diameter/2. After 5 weeks, the mice were euthanized, and their tumors were weighed. All animal protocols were followed in accordance with the Institutional Animal Care and Use Committee of our hospital."
item2302 .
item2303 .
item2304 .
item2305 .
item2306 .
item2307 "Twelve adult female SD rats, aged 6-8 weeks and weighing 200 g, were purchased from the experimental animal center of Xuzhou Medical University, China (GRADE II). The animal experience program was approved by the Committee of institutions for Animal Protection and use (IACUC). The rats were randomly divided into control group (n = 6) and hypoxia group (n = 6). They were fed in 60% humidity for 21 days, and the hypoxia group was fed under (3% O2) hypoxia. After the establishment of the rat model, the rats were anesthetized with sevoflurane (4.5%), and their hearts and lungs were taken for follow-up study."
item2308 .
item2309 .
item2310 "For LPS-induced endotoxemia model: Eight-week-old METTL3 WT and METTL3 cKO mice were intraperitoneally injected with 20 mg/kg Lipopolysaccharide for 24h. For lung acute injury model: Eight-week-old METTL3 WT and METTL3 cKO mice were intratracheally injected with 4 mg/kg Lipopolysaccharide for 6h. To induce peritonitis, mice were intraperitoneally injected (i.p.) with 1ml sterilized 3% thioglycolate (Sigma-Aldrich, St Louis, MO), and elicited cells were harvested after 2.5 hours by peritoneal lavage with 5ml of pre-cold PBS."
item2311 "Twenty BALB/c nude mice (4-6 weeks) were randomly assigned to sh-NC, sh-METTL14, sh-METTL14+oe-NC, and sh-METTL14+oe-PLAGL2, with 5 mice in each group. Mice were raised under sterile conditions of ambient room temperature of 26-28°C, the humidity of 40-60%, and alternating day and night for 10 h/14 h. The Mice were fed sterile food and water. After 1 week of adaptive feeding, A549 cells that transfected with sh-NC, sh-METTL14, oe-NC, and oe-PLAGL2 were subcutaneously injected. The cell concentration was 5 × 106/mL, and 200 μL was injected."
item2312 "A total of 32 nude mice were randomly divided into four groups with eight mice in each group. Nude mice were subcutaneously injected with 2 × 106 cells (suspended in 100 μl of PBS) transfected with NC shRNA or SND1 shRNA into the back flank of mice. In DDP administration group, DDP (3 mg/kg per week for 2 weeks) was intraperitoneally injected into mice every 3 days. The control group received 200 μl of 0.1% DMSO. The tumor volume was measured every week for a total of 4 weeks. The mice were sacrificed after 4 weeks, then the tumors were harvested for weight measurement and other analysis. The tumor volume (mm3) was estimated with the formula (0.5 × length × width2)."
item2313 "A total of 32 nude mice were randomly divided into four groups with eight mice in each group. Nude mice were subcutaneously injected with 2 × 106 cells (suspended in 100 μl of PBS) transfected with NC shRNA or SND1 shRNA into the back flank of mice. In DDP administration group, DDP (3 mg/kg per week for 2 weeks) was intraperitoneally injected into mice every 3 days. The control group received 200 μl of 0.1% DMSO. The tumor volume was measured every week for a total of 4 weeks. The mice were sacrificed after 4 weeks, then the tumors were harvested for weight measurement and other analysis. The tumor volume (mm3) was estimated with the formula (0.5 × length × width2)."
item2314 .
item2315 .
item2316 "48 male BALB/c nu/nu mice (Slake Experimental Animal Center in Shanghai) at the age of 4 weeks were randomly divided into 8 groups (n = 6/group). All groups received subcutaneous injections of HGC-27 (2 × 106 cells in 200 μL PBS), Additionally, MSCs transfected with or without CSF2 plasmid, P-MSCs transfected with or without CSF2/IGF2BP2 inhibitor, and GC-MSCs transfected with or without CSF2/IGF2BP2 inhibitor were co-injected with HGC-27 cells in a 1:1 ratio. The mice were monitored every 2 days. Tumor volumes were calculated using the modified ellipsoidal formula: V = 1/2 (length × width2)."
item2317 .
item2318 .
item2319 .
item2320 .
item2321 .
item2322 "BALB/c nude mice (aged 4-6 weeks) were fed under specific pathogen-free conditions at 26-28°C under 40-60% humidity. For tumorigenicity assay in vivo, CAL-27 cells transfected with BATF2 overexpression plasmids or empty plasmids or untreated CAL-27 cells were subcutaneously inoculated into the right armpit of mice. Following 4 weeks, mice were sacrificed by cervical dislocation under isoflurane anesthesia and neoplasms were isolated and weighted. The volume of neoplasms was monitored once a week. For the in vivo metastasis model, the tail vein of mice was intravenously injected with BATF2-overexpressed or control CAL-27 cells or untreated CAL-27 cells."
item2323 "Briefly, two million UM-SCC-1 cells stably expressing shRNA scramble or shRNAs against RBM33 and ALKBH5 were subcutaneously injected into two flanks of each NSGS mice. Tumor volume and mice weight measurements were taken every 4 days and 7 days respectively. For the in vivo ALKBH5 inhibition assay, the HNSCC PDX tumors TM01145 and TM01420 were purchased from Jackson Laboratory. 15 mg/kg 2,4-PDCA was given i.p. every four days when tumor volume reaches ~100 mm3. And, tumor volume was calculated according to the formula: [D×(d2)] /2 where D represents the large diameter of the tumor and d represents the small diameter of the tumor. Animals were individually monitored throughout the experiment."
item2324 "Wenty mice were allocated into four groups, n = 5 in each group. CSC cells transfected with sh-IGF2BP1, sh-NC, sh-IGF2BP1 + oe-BUB1B, or sh-IGF2BP1 + oe-NC were injected into mice subcutaneously (2 × 106 cells per mouse). The tumor size was detected every 4 d and evaluated by the formula below: volume = (tumor length × tumor width2)/2. On day 24, the animals were euthanized by injection of overdosed barbiturate (150 mg/kg) to have the tumors collected and weighed. All operators had no idea of the grouping details when analyzing the results."
item2325 .
item2326 .
item2327 .
item2328 .
item2329 "The animals were maintained in pathogen-free conditions at 21°C ± 2°C and 55% ± 5% humidity with free access to food and water. Mice were randomly divided into three groups (n = 8 per group) and received a subcutaneous injection of 2 × 106 stably transfected SiHa cells containing the indicated lentivirus (empty, Lv-ALKBH5, Lv-ALKBH5 + Lv-ACC1) diluted in PBS in the left flank. The mice were sacrificed when tumours were apparent on day 30. Tumour volume was recorded 7, 14, 21 and 28 days after injection with a Vernier calliper. After euthanasia, xenografts were excised from mice and weighed."
item2330 "The animals were maintained in pathogen-free conditions at 21°C ± 2°C and 55% ± 5% humidity with free access to food and water. Mice were randomly divided into three groups (n = 8 per group) and received a subcutaneous injection of 2 × 106 stably transfected SiHa cells containing the indicated lentivirus (empty, Lv-ALKBH5, Lv-ALKBH5 + Lv-ACC1) diluted in PBS in the left flank. The mice were sacrificed when tumours were apparent on day 30. Tumour volume was recorded 7, 14, 21 and 28 days after injection with a Vernier calliper. After euthanasia, xenografts were excised from mice and weighed."
item2331 "A total of 5 × 106 SW480 cells/100 μL of PBS were subcutaneously injected into the back of mice to establish a xenograft model, and tumor growth was measured with a vernier caliper every 3 days. 72 h after tumor inoculation, mice were injected intraperitoneally with 5-FU (6 mg/kg; Sigma, German) twice weekly for 21 days (6 doses in total)."
item2332 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
item2333 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
item2334 "AsPC-1 cells suspended in 100 μl of PBS (2 × 106 cells/100 μl) were injected subcutaneously into the lateral flank of the mice, which were randomly divided into solvent group (n = 6), 1.0 mg/kg of celastrol group (n = 6) and 3.0 mg/kg of celastrol group (n = 6). The administration of celastrol was performed by intraperitoneal injection into tumor-bearing mice every 2 day after 10 day inoculation. The tumor sizes were monitored with calipers every 5 days, and the tumor volume was calculated with the formula: Volume (mm3) = 1/2 × length × width2."
item2335 "AsPC-1 cells suspended in 100 μl of PBS (2 × 106 cells/100 μl) were injected subcutaneously into the lateral flank of the mice, which were randomly divided into solvent group (n = 6), 1.0 mg/kg of celastrol group (n = 6) and 3.0 mg/kg of celastrol group (n = 6). The administration of celastrol was performed by intraperitoneal injection into tumor-bearing mice every 2 day after 10 day inoculation. The tumor sizes were monitored with calipers every 5 days, and the tumor volume was calculated with the formula: Volume (mm3) = 1/2 × length × width2."
item2336 "AsPC-1 cells suspended in 100 μl of PBS (2 × 106 cells/100 μl) were injected subcutaneously into the lateral flank of the mice, which were randomly divided into solvent group (n = 6), 1.0 mg/kg of celastrol group (n = 6) and 3.0 mg/kg of celastrol group (n = 6). The administration of celastrol was performed by intraperitoneal injection into tumor-bearing mice every 2 day after 10 day inoculation. The tumor sizes were monitored with calipers every 5 days, and the tumor volume was calculated with the formula: Volume (mm3) = 1/2 × length × width2."
item2337 "AsPC-1 cells suspended in 100 μl of PBS (2 × 106 cells/100 μl) were injected subcutaneously into the lateral flank of the mice, which were randomly divided into solvent group (n = 6), 1.0 mg/kg of celastrol group (n = 6) and 3.0 mg/kg of celastrol group (n = 6). The administration of celastrol was performed by intraperitoneal injection into tumor-bearing mice every 2 day after 10 day inoculation. The tumor sizes were monitored with calipers every 5 days, and the tumor volume was calculated with the formula: Volume (mm3) = 1/2 × length × width2."
item2338 .
item2339 "A total of 24 female C57/BL6 mice aged 6 weeks were randomly assigned to the following 4 groups (n = 6 each): Group 1, PBS; Group 2, LPS (25 mg/kg); Group 3, shCtrl (1 × 107 TU) + LPS (25 mg/kg); Group 4, shMettl3 (1 × 107 TU) + LPS (25 mg/kg). The regents were administered subcutaneously over the sagittal midline of the murine calvaria. LPS was injected every three days for 6 days in Groups 2, 3 and 4, while PBS was injected in Group 1. Lentivirus (1 × 107 TU) carrying the negative control shRNA or Mettl3 shRNA was injected 2 days before the first LPS treatment in Group 3 and Group 4. Mice were sacrificed after 6 days of LPS injection and their calvaria were separated and fixed with 4% paraformaldehyde for 2 days."
item2340 "A total of 24 female C57/BL6 mice aged 6 weeks were randomly assigned to the following 4 groups (n = 6 each): Group 1, PBS; Group 2, LPS (25 mg/kg); Group 3, shCtrl (1 × 107 TU) + LPS (25 mg/kg); Group 4, shMettl3 (1 × 107 TU) + LPS (25 mg/kg). The regents were administered subcutaneously over the sagittal midline of the murine calvaria. LPS was injected every three days for 6 days in Groups 2, 3 and 4, while PBS was injected in Group 1. Lentivirus (1 × 107 TU) carrying the negative control shRNA or Mettl3 shRNA was injected 2 days before the first LPS treatment in Group 3 and Group 4. Mice were sacrificed after 6 days of LPS injection and their calvaria were separated and fixed with 4% paraformaldehyde for 2 days."
item2341 .
item2342 .
item2343 .
item2344 .
item2345 .
item2346 .
item2347 .
item2348 .
item2349 1 × 107 ME180 cells with stable overexpression of HNF1alpha or negative control ME180 cells were resuspended in 100 μL of phosphate-buffered saline and mixed with 100 μL of Matrigel.
item2350 "For compound treatment, mice were intraperitoneally injected with methyl piperidine-3-carboxylate (MP3C) (Shanghai Aladdin Biochemical Technology) at 2.5 mg/kg every 3 days for 2 weeks."
item2351 .
item2352 .
item2353 "Five-week-old male BALB/c nude mice were used for tumor growth studies in vivo. Briefly, AGS cells were subcutaneously injected into the dorsal side of mice blindly and randomly (n = 5 per group)."
item2354 "Five-week-old male BALB/c nude mice were used for tumor growth studies in vivo. Briefly, AGS cells were subcutaneously injected into the dorsal side of mice blindly and randomly (n = 5 per group)."
item2355 .
item2356 .
item2357 .
item2358 .
item2359 .
item2360 .
item2361 "Tumor cells derived xenograft (MHCC97H cells) from subcutaneous mouse model were cut into 1 mm3 pieces and implanted into the left lobe of the livers of six-week-old male NCG mice. Once the tumors were established and reached approximately 100 mm3, mice were randomly assigned to daily treatment with vehicle, lenvatinib (4 mg/kg, oral gavage), STM2457 (50 mg/kg, intraperitoneal injection) or a drug combination in which each compound was administered at the same dose and duration as the single agent."
item2362 "Male type 2 diabetic mice (db/db) [18-week-old] were purchased from SLAC (Shanghai, China) (n = 8). Corresponding 18-week-old heterozygotes mice (db/m) were used as controls. All mice were killed, and sciatic nerves were collected and stored at - 80 °C."
item2363 .
item2364 "For tumor xenograft models, QGP-1cells (5 × 106) with ALKBH5 over-expression, ALKBH5 over-expression with FABP5 knockdown, and negative control were subcutaneously injected into the right axilla of female BALB/c nude mice (4-6 weeks). After 4 weeks, the mice were sacrificed via a form of euthanasia."
item2365 "For tumor xenograft models, QGP-1cells (5 × 106) with ALKBH5 over-expression, ALKBH5 over-expression with FABP5 knockdown, and negative control were subcutaneously injected into the right axilla of female BALB/c nude mice (4-6 weeks). After 4 weeks, the mice were sacrificed via a form of euthanasia."
item2366 .
item2367 "For the experiments, mice were injected subcutaneously in the axilla with 1 × 107 MCF-7 cells with stable expression of relevant plasmids. When the tumour diameter reached approximately 5 mm, nude mice were randomly divided into two groups (six mice per group). Xenografted mice were then administrated with PBS or DOX (5 mg/kg each mouse every 3 days)."
item2368 "BALB/c male nude mice aged 6 weeks were randomly divided into two groups (n = 5 / group). A549 cells with transfection of sh-NC or sh-NEAT1 were injected into mice. After 28 days, the mice were euthanized and the tumor tissues were collected and measured."
item2369 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
item2370 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
item2371 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
item2372 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies."
item2373 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies."
item2374 "Oe-STAG3 and sh-METTL3 were transfected into HCT116 cells. Trypsin digestion and cell counting were performed on each set of cells after they had reached around 80% confluence. Nude mice were subcutaneously injected with 200 μL cells (2 × 106) and randomly assigned to 5 groups (n = 6 in each group): control group (mice were injected with cells without transfection plasmid), oe-NC group (mice were injected with cells transfected with oe-NC), oe-STAG3 group (mice were injected with cells transfected with oe-STAG3), oe-STAG3 + sh-NC group (mice were injected with cells transfected with oe-STAG3 and sh-NC) and oe-STAG3 + sh-METTL3 group."
item2375 "Oe-STAG3 and sh-METTL3 were transfected into HCT116 cells. Trypsin digestion and cell counting were performed on each set of cells after they had reached around 80% confluence. Nude mice were subcutaneously injected with 200 μL cells (2 × 106) and randomly assigned to 5 groups (n = 6 in each group): control group (mice were injected with cells without transfection plasmid), oe-NC group (mice were injected with cells transfected with oe-NC), oe-STAG3 group (mice were injected with cells transfected with oe-STAG3), oe-STAG3 + sh-NC group (mice were injected with cells transfected with oe-STAG3 and sh-NC) and oe-STAG3 + sh-METTL3 group."
item2376 .
item2377 "A549 cells (3 × 105 cells in 200 μl PBS) were subcutaneously injected into the nude mice to establish tumors. When the tumors had grown to almost 100 mm3, a 5-GyE dosage of carbon-ion irradiation was applied and the mice were examined every 3 days, then sacrificed 30 days after the injection."
item2378 "Sixty mice were disposed in 6 groups randomly by using spss25.0 software after an adaption period of one week (n = 10): control, DSS model, 5-ASA (200 mg/kg) +DSS, coptisine 25 mg/kg + DSS (COP-l), coptisine 50 mg/kg + DSS (COP-M) and coptisine 100 mg/kg + DSS (COP-H). The dosages of 5-ASA and COP were implemented in accordance with the previous report."
item2379 .
item2380 .
item2381 .
item2382 .
item2383 "The in vivo assay was approved by the Animal Care and Use Committee of Anhui Medical University. And all experimental procedures and animal care were in accordance with the institutional ethics guidelines for animal experiments. The C57BL/6 mice (male, 8-10 weeks old) were housed (six per cage) in a specific and opportunistic pathogen-free facility and maintained on a 12-hlight-dark cycle with casually access to food and water. Detailed descriptions of the cell culture, cardiac fibrosis model, and treatment with lentiviral, were given in the Supplementary methods online."
item2384 "The in vivo assay was approved by the Animal Care and Use Committee of Anhui Medical University. And all experimental procedures and animal care were in accordance with the institutional ethics guidelines for animal experiments. The C57BL/6 mice (male, 8-10 weeks old) were housed (six per cage) in a specific and opportunistic pathogen-free facility and maintained on a 12-hlight-dark cycle with casually access to food and water. Detailed descriptions of the cell culture, cardiac fibrosis model, and treatment with lentiviral, were given in the Supplementary methods online."
item2385 "The mice were divided into four groups: sham group, cecal ligation and puncture (CLP) group, CLP + lentivirus short hairpin RNA NC (lv-shNC) group, CLP + lentivirus short hairpin RNA METTL14 (lv-shMETTL4) group. The CLP assay was used to establish the sepsis-induced ALI model. In brief, mice were fasted for 12 h before surgery and were anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL/kg). Mice were fixed in a supine position, and after abdominal disinfection, an incision of about 1 cm was made along the midline of the abdominal wall. Then, the cecum was separated and penetrated using 18-gauge needle for three times. After that, the punctured cecum was returned to the abdominal cavity, and the abdominal incision was sutured layer by layer. The mice in the sham group were subjected to the same procedure without puncture treatment. For METTL14 knockdown globally, lentivirus (lv) containing shMETTL14 and shNC (0.2 ml, 1 × 109 pfu/ml) were injected into the caudal vein 4 days before modeling, respectively."
item2386 "To establish the xenograft model of GSCs in mice, GSCs or METTL3 knocking-down GSCs (v/v = 1:1) were subcutaneously inoculated into the right posterior limb of BALB/c nude mice (5 × 106 cells/mice, 4-week-old, female, body weight of 20 g). Tumor volume was measured with calipers every 3 days. The tumor size was calculated using the formula: (a2 × b)/2 (a: width in mm, b: length in mm). After about 27 days, all mice were sacrificed under general anesthesia and the xenografts were harvested for further pathological study."
item2387 "To establish the xenograft model of GSCs in mice, GSCs or METTL3 knocking-down GSCs (v/v = 1:1) were subcutaneously inoculated into the right posterior limb of BALB/c nude mice (5 × 106 cells/mice, 4-week-old, female, body weight of 20 g). Tumor volume was measured with calipers every 3 days. The tumor size was calculated using the formula: (a2 × b)/2 (a: width in mm, b: length in mm). After about 27 days, all mice were sacrificed under general anesthesia and the xenografts were harvested for further pathological study."
item2388 .
item2389 .
item2390 .
item2391 .
item2392 .
item2393 .
item2394 "5-week-old male athymic BALB/C nude mice were obtained from Experimental animal center of Fujian Medical University, and subsequently randomly divided into four groups (5 mice per group), including control, metformin, pcDNA-METTL3 and pcDNA-METTL3 + metformin. For the control or metformin group, to induce tumors, 5 × 105 H929 cells were suspended in 0.2 ml phosphate buffered saline and inoculated subcutaneously into the peritoneum of mice. Once the subcutaneous nodules grown to a rice grain size (required approximately a week), the subcutaneous xenograft model of MM in nude mice was successfully constructed. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. For the pcDNA-METTL3 group and pcDNA-METTL3 + metformin group, firstly, we transfected pcDNA-METTL3 into H929 cells to overexpress METTL3, and then 5 × 105 H929 cells were suspended in 0.2 ml phosphate-buffered saline and inoculated subcutaneously into the peritoneum of mice. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. All nude mice were closely monitored for tumor growth, skin condition, and behavior daily and any tumor ulceration or irritation was noted. Tumor length and width were calculated with vernier calipers every 7 days. After 35 days, the mice were humanely sacrificed, and the subcutaneous tumors were excised and removed."
item2395 "5-week-old male athymic BALB/C nude mice were obtained from Experimental animal center of Fujian Medical University, and subsequently randomly divided into four groups (5 mice per group), including control, metformin, pcDNA-METTL3 and pcDNA-METTL3 + metformin. For the control or metformin group, to induce tumors, 5 × 105 H929 cells were suspended in 0.2 ml phosphate buffered saline and inoculated subcutaneously into the peritoneum of mice. Once the subcutaneous nodules grown to a rice grain size (required approximately a week), the subcutaneous xenograft model of MM in nude mice was successfully constructed. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. For the pcDNA-METTL3 group and pcDNA-METTL3 + metformin group, firstly, we transfected pcDNA-METTL3 into H929 cells to overexpress METTL3, and then 5 × 105 H929 cells were suspended in 0.2 ml phosphate-buffered saline and inoculated subcutaneously into the peritoneum of mice. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. All nude mice were closely monitored for tumor growth, skin condition, and behavior daily and any tumor ulceration or irritation was noted. Tumor length and width were calculated with vernier calipers every 7 days. After 35 days, the mice were humanely sacrificed, and the subcutaneous tumors were excised and removed."
item2396 "5-week-old male athymic BALB/C nude mice were obtained from Experimental animal center of Fujian Medical University, and subsequently randomly divided into four groups (5 mice per group), including control, metformin, pcDNA-METTL3 and pcDNA-METTL3 + metformin. For the control or metformin group, to induce tumors, 5 × 105 H929 cells were suspended in 0.2 ml phosphate buffered saline and inoculated subcutaneously into the peritoneum of mice. Once the subcutaneous nodules grown to a rice grain size (required approximately a week), the subcutaneous xenograft model of MM in nude mice was successfully constructed. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. For the pcDNA-METTL3 group and pcDNA-METTL3 + metformin group, firstly, we transfected pcDNA-METTL3 into H929 cells to overexpress METTL3, and then 5 × 105 H929 cells were suspended in 0.2 ml phosphate-buffered saline and inoculated subcutaneously into the peritoneum of mice. One week later, PBS or Metformin was diluted in drinking water (2 mg/ml) and administered orally throughout the indicated time periods. All nude mice were closely monitored for tumor growth, skin condition, and behavior daily and any tumor ulceration or irritation was noted. Tumor length and width were calculated with vernier calipers every 7 days. After 35 days, the mice were humanely sacrificed, and the subcutaneous tumors were excised and removed."
item2397 .
item2398 "Male BALB/C nude mice that were four weeks old were acquired from Hunan SJA Experimental Animal Co., Ltd. and then arbitrarily categorized into four groups. These groups included NC (inoculation with A549 cells transfected with si-NC and oe-NC plasmids), si-IGF2BP2 (inoculation with A549 cells transfected with si-IGF2BP2 and oe-NC plasmid), oe-SIK (inoculation with A549 cells transfected with oe-SIK and si-NC plasmid), and si-IGF2BP2+oe-SIK2 (inoculation with A549 cells transfected with si-IGF2BP2 and oe-SIK2 plasmids). Subcutaneous injection of roughly 1 × 106 cells per nude mouse was utilized to establish the xenograft tumor in each group. Every 7 days, tumor volume was assessed using the following formula: 1/2 × length × width2. Tumor weight, on the other hand, was recorded 24 days after inoculation ."
item2399 .
item2400 .
item2401 "Four-six-week-old male BALB/c nude mice were supplied by Vital River Laboratory Animal Technology Company (Beijing, China) and fed in a specific pathogen-free environment with a temperature of 25°C and a humidity of 60%. 2 × 106 Hela cells stably transfected with pSilencer vector or pSilencer/FTO were inoculated subcutaneously into the flanks of the nude mice. After 7 days, the width and length measurement of tumors was performed every 2 days. Nude mice were killed at 28 days after cell inoculation and then tumor tissues were weighed."
item2402 "Four-six-week-old male BALB/c nude mice were supplied by Vital River Laboratory Animal Technology Company (Beijing, China) and fed in a specific pathogen-free environment with a temperature of 25°C and a humidity of 60%. 2 × 106 Hela cells stably transfected with pSilencer vector or pSilencer/FTO were inoculated subcutaneously into the flanks of the nude mice. After 7 days, the width and length measurement of tumors was performed every 2 days. Nude mice were killed at 28 days after cell inoculation and then tumor tissues were weighed."
item2403 .
item2404 .
item2405 .
item2406 "In the therapeutic study, TIALD stable knockdown or control cells were further infected by lentiviruses (Ubi-MCS-firefly_Luciferase-SV40-neomycin, Genechem, China) and selected by G418 (500 μg/mL). 45 B-NDG mice were divided into 3 groups, including the control group (n = 15), TIALD knockdown group (n = 15) and Alisertib treatment group (n = 15). The modified control cells described above were injected into the mice of control group via tail vein, while the modified TIALD knockdown cells were injected into the mice of TIALD knockdown group and Alisertib treatment group."
item2407 "Cg-Prkdcscid Il2rgtm1Vst/Vst (NPG) mice (6 weeks old) were divided into three groups and implanted with 4 × 106 cells of control (MV4-11 NC) or FTO-knockdown (MV4-11 KD-1 or KD-2) by tail vein injection, respectively."
item2408 .
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item2410 .
item2411 .
item2412 .
item2413 .
item2414 "Briefly, the 8-week-old C57BL/6 male mice were fed with high-fat diet for 4 weeks and then intraperitoneally injected STZ (Sigma-Aldrich, 50 mg/kg STZ after fasting for 6 hours). The STZ injection was conducted for 5 days while the mice drank 10% glucose solution during the treatment. The control mice were injected citrate buffer and fed normal chow. All mice were maintained on their respective diets till sacrifice. Fasting blood glucose was tested 1 week after the last STZ injection, and values of more than 16.7 mmol/L were considered diabetic."
item2415 .
item2416 .
item2417 .
item2418 .
item2419 .
item2420 .
item2421 .
item2422 "Nude mice were assigned into four groups (control, sh-NC + LV-NC, sh-METTL3 + LV-NC, and sh-METTL3 + LV-PTCH1) by the random number table method with six mice per group. After nude mice were anesthetized by intraperitoneal injection of sodium pentobarbital (60 mg/kg), the right dorsal skin was routinely disinfected with iodophor. The control mice were injected with untreated KYSE-30 cell suspensions (1 × 106 cells), and the remaining three groups were injected with equal amounts of transfected KYSE-30 cell suspensions (1 × 106 cells). After injection, the syringe was slowly withdrawn and the injection site was gently pressed with an alcohol cotton ball for 10 s to prevent cell suspension from flowing out. Ten days later, tumor volumes were measured every 3 days."
item2423 "Briefly, the mice were anesthetized and placed in a stereotactic frame. A burr hole was drilled into the skull (1.0 mm posterior and 3.0 mm lateral to the bregma). A 50 μL microinjector was used to inject 10 μL of 1 × 106 U251 cells suspended in the serum-free DMEM. The injection was performed slowly within 10 min and stayed for 10 min. After that, mice in cages and a cat were placed in the same room for fear stress induction ."
item2424 "In the xenograft tumor model in the nude mice, 5 × 106 A549 cells were mixed with 40 μg serum-EVs-oe-NC or serum-EVs-oe-HNRNPC, respectively, and then inoculated subcutaneously in the left armpit of BALB/c nude mice. / In the tumor metastasis model in nude mice, 1 × 106 A549 cells were injected into BALB/c nude mice via the tail vein. The nude mice were randomly treated with serum-EVs-oe-NC or serum-EVs-oe-HNRNPC; 20 mg EVs were injected via the tail vein once a week for 4 weeks."
item2425 .
item2426 .
item2427 "For subcutaneous xenotransplantation, 3- to 4-week-old male BALB/c nude mice were randomly divided into groups (8 mice per group) and injected in the back flank with 100 μL of 1.0 × 107 suspended cells."
item2428 "For subcutaneous xenotransplantation, 3- to 4-week-old male BALB/c nude mice were randomly divided into groups (8 mice per group) and injected in the back flank with 100 μL of 1.0 × 107 suspended cells."
item2429 "For subcutaneous xenotransplantation, 3- to 4-week-old male BALB/c nude mice were randomly divided into groups (8 mice per group) and injected in the back flank with 100 μL of 1.0 × 107 suspended cells."
item2430 "For treatment of orthotopic xenografts, 1 × 106 luciferase-labeled idC-A5-sgFZR1 PDAC cells were injected into the pancreas of 6-week-old mice. Fourteen days after implantation, the mice were randomized into three groups and received the same treatment as the subcutaneous xenograft model for 56 days. Tumor burden was monitored by bioluminescence imaging in a Living Image system (PerkinElmer) twice a week, and the mouse survival time was recorded from the day of tumor implantation to the date of death."
item2431 .
item2432 .
item2433 .
item2434 "Female BALB/c nude mice were randomly divided into three groups (n = 5 of each group) and received neck subcutaneous injection of TPC-1 cell with stable expression of ZC3H13 and IQGAP1 genes. TPC-1 cells were prepared with a density of 1 × 107/mL cell suspension using a complete medium and 0.2 mL of cell suspension was used for injection. After injection, the volume of xenograft was measured every five days, and mice were sacrificed on day 30 to obtain the tumor."
item2435 .
item2436 .
item2437 "All rats were arranged into 4 groups, with 10 rats in each group: sham, IR, IR + adeno-associated virus-mediated YTHDF2 overexpression vector (AAV-YTHDF2), and IR + negative control of AAV-YTHDF2 (AAV-NC) groups. Rats in the sham group underwent the same procedure without ligation. Rats in the IR group experienced 30-min ischemia and then 24-h reperfusion."
item2438 .
item2439 "6-week old immunodeficient mice (Guangdong Medical Laboratory Animal Center, Guangzhou, China) were selected for generating a subcutaneous xenograft model. MDA-MB-231/DOX cells were implanted subcutaneously into the immunodeficient mice. 7 days later, mice were randomly divided into 4 groups, administrated with vehicle control, FOXO1 inhibitor AS1842856 (20 mg/kg/day, i. p.), Doxorubicin (5 mg/kg/day, i. p.), and AS1842856 combined with Doxorubicin, respectively. Tumor formation was examined every 4 days."
item2440 .
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item2444 .
item2445 .
item2446 "Six BALB/c nude mice (male, 4 weeks) were randomly divided into 2 groups and inoculated with BXPC-3 cells (1 × 106) transfected with shPYGB or shNC by subcutaneous injection. Tumor volume was recorded every 7 days and mice were sacrificed after 6 weeks. To evaluate metastasis in vivo, transfected cells (5 × 106) were injected into nude mice through the tail vein."
item2447 "Six BALB/c nude mice (male, 4 weeks) were randomly divided into 2 groups and inoculated with BXPC-3 cells (1 × 106) transfected with shPYGB or shNC by subcutaneous injection. Tumor volume was recorded every 7 days and mice were sacrificed after 6 weeks. To evaluate metastasis in vivo, transfected cells (5 × 106) were injected into nude mice through the tail vein."
item2448 .
item2449 "The GIST-T1 cells were injected into the back flanks of the C57BL/6 female mice (Age six-week-old) at the concentration of 1 × 106 cells diluting in 200 μl PBS buffer solution. The GIST-T1 cells were allowed to grow in mice for a total of 25 days, the mice were sacrificed at day 25 and the tumors were obtained and weighed to evaluate the tumorigenesis abilities of the GIST-T1 cells in vivo."
item2450 "For mice in each group, 1 × 107 cells in 200 μL of PBS were injected into the subcutaneous tissue on the right flank. After injection, tumor parameters were measured every 2 days, and the tumor volume was calculated as (length × width × width)/2."
item2451 "Approximately 2 × 106 Farage/R or Farage/S cells stably transfected with shNC, shC1qA or shMETTL3 were subcutaneously injected into the left flank of each mouse. When the tumor volume reached ~100 mm3, each mouse received an intraperitoneal injection of Rituximab (20 mg/kg) every 4 days for a total of 5 injections. The diameter of each tumor was examined every 4 days using a caliper, and tumor volume was calculated as follows: (length × width2)/2. At 28 days after xenograft, the mice were sacrificed and the tumors were weighed and collected."
item2452 "Approximately 2 × 106 Farage/R or Farage/S cells stably transfected with shNC, shC1qA or shMETTL3 were subcutaneously injected into the left flank of each mouse. When the tumor volume reached ~100 mm3, each mouse received an intraperitoneal injection of Rituximab (20 mg/kg) every 4 days for a total of 5 injections. The diameter of each tumor was examined every 4 days using a caliper, and tumor volume was calculated as follows: (length × width2)/2. At 28 days after xenograft, the mice were sacrificed and the tumors were weighed and collected."
item2453 .
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item2459 .
item2460 "Five week old male C57bl/6 mice (20 ± 2 g) were divided into the DKD model group and the control group. The fasting blood glucose of all the mice was measured before the experiment, and was required to be less than 7 mmol/L. The mice in the DKD model group were fed with a high-fat and high-sugar diet for 8 weeks. Then, 1 day after restoring the high-fat and high-sugar diet, the mice in the DKD model group were fasted for 16 h and injected with STZ at 50 mg/kg per day for 5 days. The fasting blood glucose was measured 2 weeks after the last injection. If the fasting blood glucose was greater than 16 mmol/L, the medium-term diabetes model had been established successfully. Then, the rat urine was collected and the ratio of urinary albumin to urinary muscle intoxication (AIb/Cr) was measured. The AIb/Cr was greater than 30 mg/g, indicating a diagnosis of DKD. The mice in the control group received standard chow and were injected with the same amount of normal saline. Subsequently, all DKD mice were randomly divided into the sh-NC group and the sh-METTL3 group. After establishment of the DKD model, the lentiviruses carrying sh-NC or sh-METTL3 (MOI = 50) were injected into the caudal vein at a dose of 1 μg/g according to the weight of mice, once a week for 8 weeks."
item2461 "Five week old male C57bl/6 mice (20 ± 2 g) were divided into the DKD model group and the control group. The fasting blood glucose of all the mice was measured before the experiment, and was required to be less than 7 mmol/L. The mice in the DKD model group were fed with a high-fat and high-sugar diet for 8 weeks. Then, 1 day after restoring the high-fat and high-sugar diet, the mice in the DKD model group were fasted for 16 h and injected with STZ at 50 mg/kg per day for 5 days. The fasting blood glucose was measured 2 weeks after the last injection. If the fasting blood glucose was greater than 16 mmol/L, the medium-term diabetes model had been established successfully. Then, the rat urine was collected and the ratio of urinary albumin to urinary muscle intoxication (AIb/Cr) was measured. The AIb/Cr was greater than 30 mg/g, indicating a diagnosis of DKD. The mice in the control group received standard chow and were injected with the same amount of normal saline. Subsequently, all DKD mice were randomly divided into the sh-NC group and the sh-METTL3 group. After establishment of the DKD model, the lentiviruses carrying sh-NC or sh-METTL3 (MOI = 50) were injected into the caudal vein at a dose of 1 μg/g according to the weight of mice, once a week for 8 weeks."
item2462 "Specific-pathogen-free (SPF) grade C57/B6 mice (6-8 weeks old) were purchased from Weitong Lihua Co. (Beijing, China). The mice were housed at constant temperature and humidity, and 12 h light/dark cycle a day and provided with adequate food and water."
item2463 "Total of 27 BALB/c nude mice were chosen and divided into three groups (n = 6): Control group (injected with PANC-1 cells), si-NC group, and si-NNT-AS1 group. For the si-NC and si-NNT-AS1 groups, PANC-1 cells transfected with si-NC and si-NNT-AS1, respectively, were injected into mice. The back of each mouse was injected with above cell suspension (2 × 104 cells in 0.2 mL). The average volume of the tumor was measured three times in each 7 days. The mice were euthanized on the 28th day, and tumor tissues were collected for related analysis. Immunohistochemistry (IHC) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining were performed. The remaining nude mice were divided into three groups (n = 3): Control, si-NC, and si-NNT-AS1 groups. Cell suspension containing 2 × 104 cells was then injected into the back of each mouse. After 7 days, mice were administered a 50-μL suspension containing 5 × 106 CD8 T cells via peritumoral injection every 3 days for a total of five injections. After the last injection, mice were euthanized on the 28th day, and sera were collected. All animal studies were approved by the Ethics Committee of Xiangya Hospital, Central South University."
item2464 .
item2465 .
item2466 .
item2467 .
item2468 .
item2469 .
item2470 .
item2471 .
item2472 .
item2473 "A total of 1 × 107 cotransfected RPMI8226 MM cells, control RPMI8226 MM cells (shNC + LV-NC), ALKBH5-depleted RPMI8226 MM cells (shALKBH5 + LV-NC), or ALKBH5-depleted-SNHG15-overexpressed RPMI8226 MM cells (shALKBH5 + LV-SNHG15), were subcutaneously injected into the right flanks of the 4-wk-old male NOD/SCID mice (n = 6 for each group) to establish a human MM-xenografted model. Tumor growth was monitored every 3 days. The mice were euthanized after 4 wk, and tumors were measured (Tumor volume = 3.14/6 × length × width2) and harvested."
item2474 "Twenty-four C57BL/6 mice, 6-8-week-old, weighing 20-25 g, were obtained from the animal center of Nanchang University. All the animals were raised in specific-pathogen-free (SPF) conditions in a 12-h light-dark cycle and supplied with free food and water. The animal experiments were conducted following the standard guidelines and permitted by the ethics committee of The First Affiliated Hospital of Nanchang University. The mice were randomly assigned into 4 groups (n = 6 per group). Mice in the control (normoxia) group were housed at 21% O2 (room air) for 4 weeks. Mice in the PAH (hypoxia) group were housed in a chamber with 10% O2 and 90% N2 for 4 weeks. The chamber has an adsorption-type oxygen concentrator to control the flow rates of compressed air and nitrogen (Teijin, Tokyo, Japan). Mice in the PAH + si-YTHDF1 group received a tail vein injection of adenovirus containing si-YTHDF1 (Ad-si-YTHDF1). Mice in the PAH + si-NC group received a tail vein injection of Ad-si-NC. The adenovirus was immediately injected when hypoxia subjection and the adenovirus dose was 1.0 × 109 PFU with 100 μL saline for each mouse."
item2475 .
item2476 .
item2477 "The mice were randomly divided into two groups of six mice per group. Huh7 cells infected with lentivirus containing hnRNPA2B1-sgRNA or NT sgRNA were injected right-back of each mouse. And 1 × 106 cells were injected into each mouse. 28 days after injection cells, the mice were sacrificed, and the tumors were isolated and photographed. All animals were approved by the Institutional Animal Care and Ethics Committee of Renji Hospital, with a maximum allowable tumor volume of 2000 mm3."
item2478 .
item2479 .
item2480 "A549 cells (5 × 106) were subcutaneously transplanted into nude mice. Tumor size was assessed every 7 days. EVs (10 μg) were injected subcutaneously into mice every 3 days. The mice were sacrificed, tumors were removed, and their weights were recorded in the fourth week. In addition, the nude mice were injected with A549 cells (2 × 106) through the tail vein, and EVs (10 μg) were injected into mice via the tail vein every 3 days. The bilateral lung tissues were resected and stained with hematoxylin-eosin."
item2481 "Seven-week nude mice (male) were obtained from Hunan SJA Laboratory Animal Co., Ltd (Hunan, Changsha) and housed under artificial light for 12 h during the day followed by 12-h dark period at night with free access to food and water. Animal procedures complied with the guidelines of the Committee on the Ethics of Animal Experiments of the Second Affiliated Hospital of Soochow University. Stable ALKBH5-overexpressed BCPAP cells were injected subcutaneously into the left flank of nude mice. After 5 weeks of inoculation, tumors were removed and weighted, and tumor size was measured every week. Xenograft tumors were lysed in RIPA buffer and the subjected for qRT-PCR and western blot detection of ALKBH5, TIAM1, Nrf2, and HO-1 as mentioned above."
item2482 "PANC-1 cells were stably transfected with LV-METTL14, LV-NC, sh-METTL14, sh-NC, or sh-LINC00941. A mixture of 2×106 cells and 100 μL PBS was injected into the spleen of every BALB/c nude mouse. After two months of housing in a sterile environment, the mice were sacrificed. Their liver tissues were removed for hematoxylin and eosin (HE) staining."
item2483 "PANC-1 cells were stably transfected with LV-METTL14, LV-NC, sh-METTL14, sh-NC, or sh-LINC00941. A mixture of 2×106 cells and 100 μL PBS was injected into the spleen of every BALB/c nude mouse. After two months of housing in a sterile environment, the mice were sacrificed. Their liver tissues were removed for hematoxylin and eosin (HE) staining."
item2484 "Female BALB/c-nu mice (6-8 weeks of age, 18-20 g) were used to investigate the pro-invasive and pro-metastatic effects of IGF2BP3 and MCM5. To assess local invasion, indicated cells were injected subcutaneously into the flanks of mice (1 × 106 cells suspended in 100 μL sterile PBS, A549-Vector left and A549-IGF2BP3 or A549-MCM5 right, n = 5), and the number of mice with local invasion was counted. To determine distant lung dissemination, 1 × 106 indicated cells were injected into the lateral tail vein (n = 5), and metastases were monitored and analyzed by bioluminescent imaging assisted with Spectrum Living Image 4.0 software (Caliper Life Sciences, Hopkinton, MA, USA). Alternatively, vector control or IGF2BP3-overexpressing A549 cells (5 × 105) were intracardiacally injected. At the indicated experimental endpoints, mice were anesthetized and sacrificed, and lung tissues were fixed in picric acid containing 4% formaldehyde. Subcutaneous tumors and lung tissues were resected, sectioned (5 mm in thickness) and histologically examined by H&E staining."
item2485 .
item2486 .
item2487 .
item2488 "Mice (6-8 weeks, male) were subjected to unilateral renal pedicle clamping for 45 min. The animals were kept on a warm pad to maintain the constant body temperature (37 °C). Then the clamps were released for reperfusion. A sham operation was performed in a similar manner, except for clamping of the renal pedicles. Different groups of animals were euthanasia under isoflurane at 12 h, 24 h, 48 h, 120 h, and 4 weeks after ischemia. For CCL28 treatment, recombinant mouse CCL28 (50 μg/kg) were administrated through tail vein injection before surgery. For anti-CD25 treatment, PC61 mAb (10 mg/kg) was injected intraperitoneally 3 days prior to ischemia. For CCL28 antibody treatment, CCL28 antibody (100 μg/per mouse) were administrated through tail vein injection 2 h before surgery. For IOX1 (Selleck, USA) treatment, IOX1 (10 mg/kg) were administrated through tail vein injection before surgery. When determining the dosage of IOX1 in treating the IRI mice, we set a concentration gradient of 0 mg/kg, 2 mg/kg, 5 mg/kg, 10 mg/kg, 20 mg/kg to detect the most appropriate concentration of IOX1."
item2489 .
item2490 "Briefly, the mice were intraperitoneally injected with 25 μg OVA mixed with 1 mg aluminum hydroxide gel was intraperitoneally injected into mice once a week for three weeks. Subsequently, nostril challenge with 500 μg OVA was performed once a day for one week. The control mice were exposed to PBS (vehicle). LV-sh-NC or LV-sh-circMIRLET7BHG (1 × 107 TU/mL, GenePharma) were injected into mice via the tail vein two days before the nostril challenge."
item2491 "Briefly, the mice were intraperitoneally injected with 25 μg OVA mixed with 1 mg aluminum hydroxide gel was intraperitoneally injected into mice once a week for three weeks. Subsequently, nostril challenge with 500 μg OVA was performed once a day for one week. The control mice were exposed to PBS (vehicle). LV-sh-NC or LV-sh-circMIRLET7BHG (1 × 107 TU/mL, GenePharma) were injected into mice via the tail vein two days before the nostril challenge."
item2492 "The mice were randomly divided into four groups (n=5 per group), labeled as OE-vector (caudal vein injection of 2×106 AN3CA cells), OE-SLERT (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells), OE-SLERT+ASO-BDNF (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells transfected with ASO-BDNF), and OE-SLERT+k252a (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells and intraperitoneal injection of 25μg/kg k252a). After 4 weeks, the lung tissues were dissected and weighted, followed by H&E staining."
item2493 "The mice were randomly divided into four groups (n=5 per group), labeled as OE-vector (caudal vein injection of 2×106 AN3CA cells), OE-SLERT (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells), OE-SLERT+ASO-BDNF (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells transfected with ASO-BDNF), and OE-SLERT+k252a (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells and intraperitoneal injection of 25μg/kg k252a). After 4 weeks, the lung tissues were dissected and weighted, followed by H&E staining."
item2494 "Injected into the mice of the flanks subcutaneously with shNC/shNRP1 MDA-MB-231(4 × 106) cells. Using calipers to examined the tumors of length and width, calculated the volumes of tumors twice every week. After 14 days, divided BALB/c nude mice randomly into four groups: control, shNRP1, local IR, and shNRP1 + local IR. Within 15 to 20 days of tumor inoculation, radiation was given at a dose of 2 Gy per day (the treatment lasted for 5 days). All experimental mice were euthanized by intraperitoneally injecting 3% pentobarbital sodium (Sigma-Aldrich, USA) at the completion of 35 days. H&E staining and immunohistochemistry staining were performed on all tumor tissues."
item2495 "A total of 1 × 107 KIAA1429 stable knockdown OCI-LY1 cells or CHST11 stable knockdown OCI-LY1 cells were injected subcutaneously into the right armpit of mice. Two investigators who were blinded to the mice allocation observed the general condition of mice and tumor growth every 2 days, measuring tumor size with a vernier caliper upon the tumor size was higher than the skin surface and recording it."
item2496 "A total of 1 × 107 KIAA1429 stable knockdown OCI-LY1 cells or CHST11 stable knockdown OCI-LY1 cells were injected subcutaneously into the right armpit of mice. Two investigators who were blinded to the mice allocation observed the general condition of mice and tumor growth every 2 days, measuring tumor size with a vernier caliper upon the tumor size was higher than the skin surface and recording it."
item2497 "For xenograft growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were inoculated subcutaneously into the axillas of nude mice. The tumor volumes were measured every 3 days. After 21 days, the mice were sacrificed. Simultaneously, the subcutaneous tumors were excised and weighed. For the lung metastatic colonization model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were injected into the tail veins of nude mice. After 5 weeks, the mice were sacrificed, with their lung tissues dissected. All subcutaneous tumors and lung tissues were paraffin embedded and sectioned for subsequent analyses."
item2498 "For xenograft growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were inoculated subcutaneously into the axillas of nude mice. The tumor volumes were measured every 3 days. After 21 days, the mice were sacrificed. Simultaneously, the subcutaneous tumors were excised and weighed. For the lung metastatic colonization model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were injected into the tail veins of nude mice. After 5 weeks, the mice were sacrificed, with their lung tissues dissected. All subcutaneous tumors and lung tissues were paraffin embedded and sectioned for subsequent analyses."
item2499 .
item2500 "8-week-old male C57BL/6 mice were randomly divided into three groups: Ctrl+AAV-NC group (n=7), MIA+AAV-NC group (n=7), and MIA+ AAV-IT1 group (n=7). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich, St. Louis, MO, USA) in the knee. Control mice were given normal saline in the same volume. One week later, 20 μL of AAV-HS3ST3B1-IT1 or AAV-NC (GeneChem, Shanghai, China) were injected into the knee joints of mice. Treatments were administered once per week for 3 consecutive weeks. Six weeks later, the mice were euthanized, and the knee joints were collected and store at -80°C."
item2501 "In order to delete Ythdc1 specifically in SC, experimental mice were administered intraperitoneally with 2 mg TMX (Sigma) dissolved in corn oil per 20 g body weight for 5 consecutive days. All mice were housed in specific pathogen-free animal facility. Procedures and protocols involving mice were approved by the Animal Care and Ethics Committee of Jinan University. If not stated specifically, 2-4 months old and gender-matched mice were used for all experiments."
item2502 "For in vivo tumor formation, AGS cells (5 × 107) stably infected with sh-METTL3 lentivirus or sh-NC control cells were injected subcutaneously into nude mice (n = 3). The volume and size of the tumors were observed and recorded every three days from the third day after inoculation, calculated as 0.5×(length×width2)."
item2503 "A total of 5×106 NC-shRNA- or RBM15-shRNA-infected A2780-PTX cells in 100 μl medium were subcutaneously injected into the right flank of mice (n=5/group). Mice without any intervention were used as blank controls (n=5). Body weight, tumor initiation and tumor progression were monitored every other day for 29 days (day of tumor formation=day 1)."
item2504 .
item2505 .
item2506 .
item2507 .
item2508 .
item2509 "Following one week of adaptive feeding, the mice were haphazardly allocated to one of two groups: sh-NC or sh-CDC45. The former received subcutaneous injection of SK-Hep-1 cells transfected with sh-NC while the latter received injection of SK-Hep-1 cells transfected with sh-CDC45. Each mouse was injected with 3 × 10 [6] cells in a volume of 150 μl at the left armpit. Tumor volume was monitored biweekly following implantation."
item2510 "The animals were first injected with JAR cells for 7 days, and the mice in AgomiR-935 group, AgomiR-NC group, AntagomiR-935 group, and AntagomiR-NC group were injected via tail vein with miR-935 agomiR (2 nmoL, Ribobio), miR-935 antagomiR (5 nmoL, Ribobio), or same dosage of negative controls (Ribobio) every 3 days for 2 weeks."
item2511 .
item2512 .
item2513 .
item2514 .
item2515 (1) Lenti-NC and Lenti-vector were injected into caudal vein in group A; (2) Lenti-NC and Lenti-RBM15 OE were injected into caudal vein in group B; (3) Lenti-E6 shRNA and Lenti-vector were injected into caudal vein in group C; (4) Lenti-E6 shRNA and Lenti-RBM15 OE were injected into caudal vein in group D. The dosage of each lentivirus tail vein injection is 108 TU/animal.
item2516 .
item2517 .
item2518 .
item2519 .
item2520 "For the tumor xenograft model, 1 × 107 gastric cancer cells were injected s.c. into 4- to 6-week-old female BALB/C nude mice (Vitalriver)."
item2521 "The nude mice were inoculated in the inguinal region subcutaneously with 1 × 106 stably transfected GC cells which were suspended in 100 μL PBS. After one week, preestablished tumor xenografts were treated with DMSO or OTS186935 (50 mg/kg, 2 × /wk × 3)."
item2522 "The nude mice were inoculated in the inguinal region subcutaneously with 1 × 106 stably transfected GC cells which were suspended in 100 μL PBS. After one week, preestablished tumor xenografts were treated with DMSO or OTS186935 (50 mg/kg, 2 × /wk × 3)."
item2523 .
item2524 .
item2525 .
item2526 .
item2527 .
item2528 .
item2529 .
item2530 .
item2531 .
item2532 "Male BALB/c nude mice (4-weeks-old; body weight 20-25 g; n=15) were obtained from Vital River Laboratories (Beijing, China) and maintained at 20-26 °C and 40-70% humidity with specific pathogen-free conditions. The mice were divided into control, TMA7 KD, and TMA7 KD+UBA2 OE groups. Each nude mouse was injected with 100 μL (1.0x107 cells) of lentivirus-transfected LSCC cells. The formula length×width 2×0.5 was used to calculate the tumor volume. 34 days after inoculation, the xenografts were excised, and their volume was determined."
item2533 .
item2534 .
item2535 "Overexpression of FTO in the liver of 8-week-old mice was achieved by tail vein injection of adenoviral FTO (Ad-FTO) or control adenovirus (Ad-Ctrl) (5 × 107 pfu/g). Two weeks after injection, mice were sacrificed and tissues were collected. HFD-fed mice were also injected with Ad-FTO or Ad-Ctrl (5 × 107 pfu/g). After 3 weeks, mice were sacrificed and tissue samples were collected."
item2536 "Overexpression of FTO in the liver of 8-week-old mice was achieved by tail vein injection of adenoviral FTO (Ad-FTO) or control adenovirus (Ad-Ctrl) (5 × 107 pfu/g). Two weeks after injection, mice were sacrificed and tissues were collected. HFD-fed mice were also injected with Ad-FTO or Ad-Ctrl (5 × 107 pfu/g). After 3 weeks, mice were sacrificed and tissue samples were collected."
item2537 "A total of 5×106 Caski cells were subcutaneously injected into the left inguen of mice. When tumors were ~50 mm3±10% (day16). The mice were randomly divided into 4 groups and received piRNA-17458 mimic or piRNA-17458 inhibitor by intratumor injection for every 4 days for a total of five injections. On the 36th day, the tumor weight and lymph node metastasis of the nude mice were assessed after mice were euthanized."
item2538 .
item2539 .
item2540 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
item2541 "A total of 2.0 × 106 SCAPs were mixed with 20 mg HA/tricalcium phosphate (Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China) at 37°C for 2 h, and then, the mixture was subcutaneously transplanted into the back of nude mice (10-week-old female, nu/nu)."
item2542 .
item2543 .
item2544 .
item2545 .
item2546 .
item2547 A total of 106 cells transfected with the IGF2BP1 silencing (sh-IGF2BP1-2) or control (sh-NC) constructs were injected subcutaneously into nude mice.
item2548 "EXOSC2 overexpression vector was transfected to MCF-7 cells, and stably infected cells (5 × 106) were injected subcutaneously into the left axilla of mice to induce BC in vivo [22]. Tumor size was examined every 3 days and calculated according to the formula."
item2549 .
item2550 .
item2551 "SW620 cells were seeded in 6-well plates and infected with USP29-knockdown lentivirus (sh-USP29) or the negative control (sh-NC), respectively, and cells were treated with 4 μg/mL of puromycin (Invitrogen) for two weeks to screen stable knockout cells. Afterward, each mouse was subcutaneously injected via the axilla with stable infected SW620 cells (N = 5 × 106 cells)."
item2552 .
item2553 .
item2554 "Male nude mice (age: 4 weeks) were obtained from Charles River (Hangzhou, Zhejiang, China). For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. Next, we detected and measured the tumor volume each week. The weight of the tumor in each nude mouse was also measured at 4 weeks after injection. Immunohistochemistry (IHC) was used to detect Ki67- and caspase-3- positive cells in the tumor."
item2555 "Male nude mice (age: 4 weeks) were obtained from Charles River (Hangzhou, Zhejiang, China). For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. Next, we detected and measured the tumor volume each week. The weight of the tumor in each nude mouse was also measured at 4 weeks after injection. Immunohistochemistry (IHC) was used to detect Ki67- and caspase-3- positive cells in the tumor."
item2556 "Male nude mice (age: 4 weeks) were obtained from Charles River (Hangzhou, Zhejiang, China). For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. Next, we detected and measured the tumor volume each week. The weight of the tumor in each nude mouse was also measured at 4 weeks after injection. Immunohistochemistry (IHC) was used to detect Ki67- and caspase-3- positive cells in the tumor."
item2557 .
item2558 "STZ-induced diabetic mice were further divided into three groups: STZ, STZ+pcDNA3.1, and STZ+METTL3. Animals in the STZ+METTL3 group were intravenously injected with pAd-METTL3 vectors (109 plaque forming unit per mouse), while those in the STZ+pcDNA3.1 group were injected with pAd-NC vectors (109 plaque forming unit per mouse). The STZ group was administered with phosphate-buffered saline following the same procedure. After another 4 weeks, all mice were euthanized. Their serum and testicular tissues were collected for further analysis."
item2559 "Female athymic BALB/C nude mice received a subcutaneous injection of 5 × 106 cells into the axilla (4 weeks old, 18-20 g). The tumor volume (V) was calculated each week using the formula V = (W2 L)/2 after measuring the tumor width (W) and length (L). After the injection of cancer cells, for the gemcitabine treatment cohort, mice were injected with gemcitabine (120 mg/kg, Sigma-Aldrich) intraperitoneally and weekly according to previous studies ."
item2560 .
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item2568 .
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item2571 .
item2572 "Leukemic BM cells (RFP+) isolated from primary RUNX1-RUNX1T1 and KITN822K leukemia mice were transduced with shNC or shAlkbh5 and 1 × 106 transduced cells were transplanted into lethally irradiated 6- to 8-week-old BALB/c recipient mice. The xenograft mouse model was established by injecting 1 × 106 Kasumi-1 cells expressing shNC or shALKBH5 into NOD scid gamma (NSG) mice via tail vein. For the human AML PDX models, 1 × 106 t (8;21) AML patient-derived bone marrow mononuclear cells (BMMNCs) expressing shNC or shALKBH5 were transplanted into NSG recipient mice intravenously."
item2573 .
item2574 .
item2575 .
item2576 .
item2577 .
item2578 "The mice were randomly grouped: (1) shNC + vehicle; (2) shNC + Erastin; (3) shWTAP + Erastin; (4) shcircCMTM3 + Erastin. Briefly, HCC cells with stable silenced WTAP and circCMTM3 (2 × 106 in 0.1 mL PBS) were injected into the left flank of the mice subcutaneously."
item2579 "The mice were randomly grouped: (1) shNC + vehicle; (2) shNC + Erastin; (3) shWTAP + Erastin; (4) shcircCMTM3 + Erastin. Briefly, HCC cells with stable silenced WTAP and circCMTM3 (2 × 106 in 0.1 mL PBS) were injected into the left flank of the mice subcutaneously."
item2580 "The cells were then washed with PBS and digested with trypsin. After counting, 1 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-6 weeks) to establish a subcutaneous implantation model. After 4 weeks, the volume and weight of the tumors were measured after the mice were sacrificed by CO2 inhalation and cervical dislocation."
item2581 "The cells were then washed with PBS and digested with trypsin. After counting, 1 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-6 weeks) to establish a subcutaneous implantation model. After 4 weeks, the volume and weight of the tumors were measured after the mice were sacrificed by CO2 inhalation and cervical dislocation."
item2582 "We injected 2 × 106 stably infected GC cells subcutaneously into each dorsal flank of mice (n = 6). After 4 weeks, each histologically intact tumor was removed, weighed and photographed. To construct the model of mice pulmonary metastasis, we injected 2 × 106 stably infected GC cells into the tail vein of the mice (n = 5)."
item2583 .
item2584 "HCT116 cells stably expressing sh-NC or sh-circ_0124554 were diluted to 2 × 106 cells in 0.2 mL phosphate buffer solution and injected into the mice, followed by 6 Gy irradiation once per day for 5 days. Ten days later, the tumor volume was calculated every 5 days for 5 cycles. Mice were sacrificed after 35 days, and the tumors were dissected for further analysis."
item2585 .
item2586 .
item2587 .
item2588 .
item2589 .
item2590 .
item2591 .
item2592 "To establish an orthotopic xenograft tumor model, 12 healthy female Balb/c nude mice were chosen and assigned to two groups. Nude mice were anesthetized by isoflurane inhalation, and after disinfecting the skin with iodophor, a 5 mm incision was made in the skin and abdominal wall, parallel and ventral to the spine, midway, and between the last rib and the iliac crest. After pulling out the ovary, the cell suspensions (10 μL) containing 1 × 106 IGF2BP2-overexpressing or control SKOV3 cells were inoculated after inserting the needle at the junction between the bursa and the fat pad. The ovary was put back in place, and if no bleeding was noted, the incision on the muscle layer and body wall was closed separately."
item2593 "In this study, we introduced an animal model of EV71 infection (C4-ZZ1350) based on 3-day-old BALB/c mice inoculated with a lethal dose of EV71 strain (2 × 106 PFU/mouse) by the intraperitoneal (i.p.) route (n = 11 per group)."
item2594 .
item2595 .
item2596 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
item2597 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
item2598 .
item2599 .
item2600 "SCLC cells were collected, and cell suspensions were prepared at a concentration of 1 × 106 cells per 100 μL PBS and injected subcutaneously into nude mice. When the tumour volume reached 60 mm3, mice were randomly divided into control and treatment groups with five mice in each group. The mice in the treatment group were treated regularly, and chemotherapy drugs (CDDP 3 mg/kg and VP16 2 mg/kg) were administered via intraperitoneal injection."
item2601 "SCLC cells were collected, and cell suspensions were prepared at a concentration of 1 × 106 cells per 100 μL PBS and injected subcutaneously into nude mice. When the tumour volume reached 60 mm3, mice were randomly divided into control and treatment groups with five mice in each group. The mice in the treatment group were treated regularly, and chemotherapy drugs (CDDP 3 mg/kg and VP16 2 mg/kg) were administered via intraperitoneal injection."
item2602 .
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item2606 .
item2607 .
item2608 "For subcutaneous inoculation, 1 × 106 control or METTL16-depleted CCA cells suspended in 100 μL PBS mixed with matrix gel (BD, 356,234) at 1:1 ratio was injected into the mice's flanks.For intrahepatic inoculation, 1 × 106 control or METTL16-depleted CCLP1 cells suspended in 10 μL PBS mixed with matrix gel (BD, 356,234) at a 1:1 ratio was implanted into the livers of NOD-SCID mice."
item2609 .
item2610 .
item2611 "2 × 107 HepG2 cells transfected with scrambled shRNA or Snhg1 shRNA were injected subcutaneously into nude mice. For evaluating the sensitivity of the formed tumors to sorafenib, 60 mg/kg sorafenib was applied in the tumor-bearing mice via oral administration daily for 14 days."
item2612 "The xenograft mouse models were established by injecting MCF-7/ADR cells (1 × 107 in 100 μL RPMI 1640 medium) into the mouse right flank. Tumor size was monitored every week. When the average tumor size reached approximately 100 mm3, 5.0 mg/kg adriamycin were subsequently subjected through tail vein every other day. Mice were sacrificed after 4 weeks, and tumors were excised."
item2613 .
item2614 .
item2615 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2616 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2617 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2618 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2619 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2620 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
item2621 .
item2622 .
item2623 .
item2624 .
item2625 "The mice were housed according to the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The corresponding cells (5 × 106) with luciferase signal were prepared and resuspended in 150 μl PBS/Matrigel (BD Biosciences, 1:1 mixture). The mice were randomly divided into 3 groups (n = 5 per group). The cells were injected into the mice's hepatic lobe. After 2 weeks, the mice were anesthetized with isoflurane and intraperitoneally injected with 150 mg/kg D-Luciferin once a week to evaluate tumor metastasis."
item2626 .
item2627 "For xenograft tumor growth assay, 5×106 NSCLC cells were subcutaneously injected into the left armpit of BALB/c nude mice (n=6 per group)."
item2628 .
item2629 Female BALB/c nude mouse (4 weeks) was used to establish a xenograft transplant model. DLD-1 cells stably transfected with sh-linc00659 or scramble were subcutaneously inoculated in the mouse with 2 × 106 cells.
item2630 .
item2631 .
item2632 .
item2633 "A549 cells (5 × 106) with LINC00641 stable knockdown or control were suspended in 200 μL of 50% Matrigel (Corning), then injected subcutaneously into the flanks of the nude mice. For the metastatic tumor model, the male BALB/c-nude mice weighing 18-23 g were randomly divided into two groups. A549 cells with LINC00641 stable knockdown or control were injected into the tail veins of the nude mice (2 × 106 cells per mice)."
item2634 "A total of 20 sixteen-weeks-old NSG mice were used for xenotransplantation experiments. After randomization, 10 mice per group were sub-lethally irradiated (250 cGy) and tail vein-injected with 5 x 105 SF3B1 5'UTR WT or A88GMUT human leukemia MOLM-13 cells. Mice were euthanized upon developing leukemia as indicated by lethargy, hunched position and other signs of sickness. Leukemic burden was monitored by checking lymphocyte counts using an automated hematology analyzer (Sysmex KX-21N) and FACS analysis of human CD45 chimerism in bone marrow."
item2635 .
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item2647 .
item2648 2 × 106 SW620 cells stably overexpressed or silencing METTL16 were injected into the right flank of mice to observe tumor growth. Tumor volumes were monitored once a week after injection and calculated using the formula 0.5 × a2 × b.
item2649 2 × 106 SW620 cells stably overexpressed or silencing METTL16 were injected into the right flank of mice to observe tumor growth. Tumor volumes were monitored once a week after injection and calculated using the formula 0.5 × a2 × b.
item2650 Twelve-week-old wild-type (WT) and IL-10-KO mice were allocated randomly to four groups (n = 5 per group): (1) the WT group; (2) the IL-10 KO + si-control group (administrated saline as a placebo via enema once); (3) the IL-10 KO + si-circPRKAR1B group (administrated 200 μL of 1e11 vg adeno-associated virus (AAV) vector carrying si-circPRKAR1B via enema once); and (4) the IL-10 KO + si-METTL3 group (administrated 200 μL of 1e11 vg AAV vector carrying si-METTL3 via enema once).
item2651 .
item2652 .
item2653 .
item2654 .
item2655 .
item2656 "A total of 1 × 106 ACHN cells was injected into left renal capsule (n = 5 mice/group). Then, we sutured the wound layer by layer. Tumour size was measured 4 weeks later with in-Vivo FX PRO small animal imaging system (BRUKER). After that, we executed the mice and collected tumours for immunohistochemistry (IHC) assays and Western blotting. For metastasis model construction, 1 × 106 ACHN cells were injected into tail-vein of each mouse (n = 5 mice/group)."
item2657 "A total of 1 × 106 ACHN cells was injected into left renal capsule (n = 5 mice/group). Then, we sutured the wound layer by layer. Tumour size was measured 4 weeks later with in-Vivo FX PRO small animal imaging system (BRUKER). After that, we executed the mice and collected tumours for immunohistochemistry (IHC) assays and Western blotting. For metastasis model construction, 1 × 106 ACHN cells were injected into tail-vein of each mouse (n = 5 mice/group)."
item2658 "A total of 1 × 106 ACHN cells was injected into left renal capsule (n = 5 mice/group). Then, we sutured the wound layer by layer. Tumour size was measured 4 weeks later with in-Vivo FX PRO small animal imaging system (BRUKER). After that, we executed the mice and collected tumours for immunohistochemistry (IHC) assays and Western blotting. For metastasis model construction, 1 × 106 ACHN cells were injected into tail-vein of each mouse (n = 5 mice/group)."
item2659 "A total of 1 × 106 ACHN cells was injected into left renal capsule (n = 5 mice/group). Then, we sutured the wound layer by layer. Tumour size was measured 4 weeks later with in-Vivo FX PRO small animal imaging system (BRUKER). After that, we executed the mice and collected tumours for immunohistochemistry (IHC) assays and Western blotting. For metastasis model construction, 1 × 106 ACHN cells were injected into tail-vein of each mouse (n = 5 mice/group)."
item2660 "A total of 1 × 106 ACHN cells was injected into left renal capsule (n = 5 mice/group). Then, we sutured the wound layer by layer. Tumour size was measured 4 weeks later with in-Vivo FX PRO small animal imaging system (BRUKER). After that, we executed the mice and collected tumours for immunohistochemistry (IHC) assays and Western blotting. For metastasis model construction, 1 × 106 ACHN cells were injected into tail-vein of each mouse (n = 5 mice/group)."
item2661 .
item2662 .
item2663 "1106 cells/100 L NSCLC cell suspension (oe-NC group, oe-METTL14 + oe-NC group, oe-METTL14 + oe-TXNIP group) were injected into the tail vein. Twenty-four nude mice (8 in each group) were employed. Following the 56th post-injection day, mice were decapitated by decortication. Hematoxylin-eosin (HE) staining was used to identify tumor metastases in the mouse lung tissue ."
item2664 .
item2665 "GC pulmonary metastatic model was established by injecting MKN-45 cells transfected with LV Sh355 lentivirus or control LV ShNC lentivirus (2 × 106 in 200 μl sterile 1 × PBS) into the caudal vein of 5-6-week-old male BALB/c nude mice (Shanghai Experimental Animal Center, China). The survival times of the two groups were recorded. One month after injection, mouse was injected with a corresponding amount of 15 mg/ml d-Luciferin (Yeasen, China) solution, 10 μl/g of body weight. Imaging analysis was performed 10-15 min after the intraperitoneal injection."
item2666 "Stably transfected GC cells (1 × 106) with sh-CENPF or CENPF overexpression were mixed in 0.1 mL PBS, and then were injected subcutaneously into the groin of 5-week-old BALB/C nude mice (n = 5 each group). After four weeks, the nude mice were sacrificed, and tumor tissues were dissected. Finally, the weight and volume of the tumors were measured. Tumor volume = length × width2 × 1/2.A total of 1 × 106 luciferase labeled GC cells were injected into the spleen of 5-week-old BALB/C nude mice. After 4-5 weeks, bioluminescence signals of liver metastases were detected via an IVIS imaging system (PerkinElmer, Norwalk, Connecticut, USA). Liver tissues were removed for hematoxylin-eosin (HE) (Beyotime, Shanghai, China) stained sections to evaluate metastatic liver lesions."
item2667 "Stably transfected GC cells (1 × 106) with sh-CENPF or CENPF overexpression were mixed in 0.1 mL PBS, and then were injected subcutaneously into the groin of 5-week-old BALB/C nude mice (n = 5 each group). After four weeks, the nude mice were sacrificed, and tumor tissues were dissected. Finally, the weight and volume of the tumors were measured. Tumor volume = length × width2 × 1/2.A total of 1 × 106 luciferase labeled GC cells were injected into the spleen of 5-week-old BALB/C nude mice. After 4-5 weeks, bioluminescence signals of liver metastases were detected via an IVIS imaging system (PerkinElmer, Norwalk, Connecticut, USA). Liver tissues were removed for hematoxylin-eosin (HE) (Beyotime, Shanghai, China) stained sections to evaluate metastatic liver lesions."
item2668 .
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item2672 "Cells (2 × 104) were seeded onto 12-well plates and cultured in serum-free 1640 medium. Cell spheroids were documented and quantified using an inverted microscope (Olympus, Japan) after two weeks."
item2673 "Cells (2 × 104) were seeded onto 12-well plates and cultured in serum-free 1640 medium. Cell spheroids were documented and quantified using an inverted microscope (Olympus, Japan) after two weeks."
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item2681 Mice were acclimated to the new environment for one week before the experiment. The mice were randomly divided into two groups as sh-HES1 group (n = 6) and sh-NC group (n = 6). Sh-HES1 HCT116 cells (5×106) and corresponding control cells were resuspended with 100 μl PBS and injected into the right subcutaneous BALB/c nude mice.
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item3212 "To investigate the effect of lactate on sepsis-induced lung injury, sodium oxalate (0.75 g/kg body weight, Selleck, S6871) [18] or 2-DG (0.5 g/kg body weight, Selleck, S4701) [19] was injected intraperitoneally 3 h before CLP or sham operation to inhibit lactate production. To suppress METTL3 levels, mice were injected with STM2457 (30 mg/kg body weight, MedChemExpress, HY-134836) 12 h before CLP or sham surgery, with a supplemental injection of STM2457 before surgery."
item3213 .
item3214 "Colorectal cancer cells were seeded in culture plates for 24 h prior to cotransfection with GFP-CARMN, and a vector using Lipofectamine 2000. After 48 h, RNA immunoprecipitation was performed using/Colorectal cancer cells were plated in 24-well plates and incubated for 24 h before cotransfection with the luciferase reporter vector, and the Renilla vector. antibodies against FTO, METTL3 and ALKBH5 from the EZ-Magna RIP-Kit (Millipore)."
item3215 1 μg of labeled RNA was incubated with 500 μg of cell lysates from WI-38 cells at the indicated PDL.
item3216 .
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item3225 "A total of 30, six-week-old male C57/BL6 mice were purchased from Orient Bio Inc. (Beijing, China), and they were fed according to standard procedures. After one week of adaptive feeding, the mice were randomly divided into two groups, namely, the control group (n = 7) and the UVR group (n = 7). All mice were shaved once a week. The UVR mice were irradiated with a mixed source of UVA (315 nm ~ 400nm, 0.60 mW/cm2) and UVB (290nm ~ 315nm, 3.5 mW/cm2) ray every other day for 12 weeks. The initial irradiation time was 15 min for the first week base on the minimal erythema dose (MED), followed by a graduated increase until it reached 80 min. The total irradiated dose was approximately 151 J/cm2 for UVA and 23 J/cm2for UVB, respectively."
item3226 "Male C57BL/6 mice (22-25 g; Animal Center of the Second Affiliated Hospital of Harbin Medical University) were housed under a 12-h light/dark cycle at a temperature of 22-24 °C, humidity between 50 ± 10%, and with ad libitum access to food and water. The mice were acclimatized for at least 1 week before the experiments. Type 1 DM was induced by intraperitoneally injecting streptozotocin (STZ) at a dose of 55 mg/kg for 5 days consecutively. Mice that received STZ injections were considered diabetic when their blood glucose levels consistently surpassed 16.7 mmol/L (300 mg/dL) for two days consecutively. Diabetic mice were used 4 months post-last STZ injection, with blood glucose levels above 16.7 mmol/L. Any mice that did not exhibit DM development were eliminated from the experiment. The control group of mice (CON) was administered a comparable dosage of citric acid buffer .Four weeks after successful STZ induction, AAV2sh-METTL3 (2 μL; 1 × 1013 vg/mL; WZ Biosciences Inc., China) was injected into the vitreous humor of the right eyes of these mice using a 30-gauge needle and a microinjector (Hamilto Sigma-Aldrich, St.Louis, MO, USA), forming the 'STZ + AAV2sh-METTL3' group. Mice injected with AAV2sh-NC were designated as the 'STZ + AAV2sh-NC' group. At the same time, the left eye was administered 2μL saline and was designated as the 'STZ' group. The eyes of control mice were administered the same saline dosage and were designated as the 'Control-sham' group. Mice were euthanized at 4 months, and their eyes were removed. The Second Affiliated Hospital Ethics Committee of Harbin Medical University authorized the animal experiments (YJSDW2023-088)."
item3227 "The 6-weeks ages of male BALB/c Nude Mouse were used in this study.To establish the xenograft model, 5 ×106 U87 control cells or U87 IGF2BP3-KD cells were subcutaneously injected into the rear flank of nude mice (four mice per group). Tumor growth was monitored every two days by measuring the tumor size using a caliper. Once the diameter of the tumor reached approximately 10 mm, the mice were sacrificed, and the tumors were excised and weighed."
item3228 "The 8-week-old male Alkbh5 KI or Alkbh5 KO mice and their littermate WT mice were randomly exposed to either 21% oxygen (normoxia) or 10% oxygen (hypoxia) condition in a ventilated anoxic chamber (TOW-INT TECH Co., Ltd. Shanghai, China) for 4 weeks. The anoxic chamber was opened 2 times a week for 10 min each time for cheaning and refilling standard mouse chow and water. After 4 weeks of hypoxia period, the mice were anesthetized, hemodynamics were evaluated, followed by rapid removal of the heart and lungs at the end of the assessment."
item3229 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.5% carboxymethylcellulose sodium, as described previously."
item3230 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.6% carboxymethylcellulose sodium, as described previously."
item3231 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.5% carboxymethylcellulose sodium, as described previously."
item3232 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.6% carboxymethylcellulose sodium, as described previously."
item3233 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.5% carboxymethylcellulose sodium, as described previously."
item3234 "Mice were anesthetized using 1.25% tribromoethanol by intraperitoneal injection, and fixed in their back on a 45-degree slope. During the anesthetization, the trachea of mice was appeared using surgical instruments, and the arsenic solution was instilled followed by 50 μL air by a syringe. Then, mice were held on the head up until proper breathing was assured, and gently transferred on the 37 °C heating plate until they recovered from anesthesia. To inactivate the FTO protein activity, mice were gavaged for 10 days with Rhein (100 mg/kg) dissolved in 0.6% carboxymethylcellulose sodium, as described previously."
item3235 .
item3236 "1 × 107 cells were injected into the right axillary region of nude mice. After 28 days, the mice were sacrificed and tumors were removed. ESCA cells (2 × 107 cells) were injected into nude mice via the tail vein. After 6 weeks, the mice were sacrificed and the lungs were obtained and fixed with 4% paraformaldehyde. The number of metastatic nodules was counted following H&E staining."
item3237 .
item3238 .
item3239 .
item3240 .
item3241 "General anesthesia in mice was induced by intraperitoneal injection of a 0.3% pentobarbital sodium solution at 40 mg/kg. Excessive whiskers were trimmed, and topical anesthesia was performed using 0.5% proparacaine hydrochloride (Alcon, Geneva, Switzerland). A circular filter paper (2.0 mm × 2.0 mm) soaked with NaOH (1 mol/L) was attached to the central cornea of the right eye for 40 seconds to induce an alkali injury. Afterward, the paper was quickly removed, and the conjunctival sac was washed entirely with 0.9% sterile saline solution for one minute. Mice were then treated with ofloxacin eye drops twice daily for three days to prevent infection. Mice were randomly selected for three, seven, and 14 days or seven days after modeling, and their right corneas were harvested for subsequent experiments."
item3242 "General anesthesia in mice was induced by intraperitoneal injection of a 0.3% pentobarbital sodium solution at 40 mg/kg. Excessive whiskers were trimmed, and topical anesthesia was performed using 0.5% proparacaine hydrochloride (Alcon, Geneva, Switzerland). A circular filter paper (2.0 mm × 2.0 mm) soaked with NaOH (1 mol/L) was attached to the central cornea of the right eye for 40 seconds to induce an alkali injury. Afterward, the paper was quickly removed, and the conjunctival sac was washed entirely with 0.9% sterile saline solution for one minute. Mice were then treated with ofloxacin eye drops twice daily for three days to prevent infection. Mice were randomly selected for three, seven, and 14 days or seven days after modeling, and their right corneas were harvested for subsequent experiments."
item3243 "General anesthesia in mice was induced by intraperitoneal injection of a 0.3% pentobarbital sodium solution at 40 mg/kg. Excessive whiskers were trimmed, and topical anesthesia was performed using 0.5% proparacaine hydrochloride (Alcon, Geneva, Switzerland). A circular filter paper (2.0 mm × 2.0 mm) soaked with NaOH (1 mol/L) was attached to the central cornea of the right eye for 40 seconds to induce an alkali injury. Afterward, the paper was quickly removed, and the conjunctival sac was washed entirely with 0.9% sterile saline solution for one minute. Mice were then treated with ofloxacin eye drops twice daily for three days to prevent infection. Mice were randomly selected for three, seven, and 14 days or seven days after modeling, and their right corneas were harvested for subsequent experiments."
item3244 "Sh-GID8 stable cells (2 × 106) were inoculated subcutaneously into the left axilla of athymic nude mice (n = 5 mice/group). Every three days, the tumor's size was measured with a digital caliper, and its volume was computed using the following formula: tumor volume (mm3) = length × width × width × 0.52. When the subcutaneous tumor reached approximately 50-100 mm3, glutamine (75 mg/kg) [13] or PBS was injected intratumorally every day. Tumors were collected for TUNEL staining or other analysis when tumor volumes reached the humane endpoint. For the metastasis model, the constructed HCT116 cells (2 × 106) with stable YTHDF1 or GID8 knockdown were injected into nude mice through the tail vein (n = 4 or n = 5 mice/group). Two months later, all the mice were sacrificed to observe tumor metastasis in the lungs."
item3245 "Mice were treated with a single i.p. injection of pentylenetetrazol (PTZ, Sigma-Aldrich, P6500) at a dose of 50 mg/kg to establish the animal model of acute seizures [16]. Betaine (Sigma-Aldrich, B2629) was dissolved in normal saline, and administrated at a dose of 200 mg/kg or 600 mg/kg, i.p., to mice for 14 days before PTZ injection in reference to a previous study."
item3246 "Mice were treated with a single i.p. injection of pentylenetetrazol (PTZ, Sigma-Aldrich, P6500) at a dose of 50 mg/kg to establish the animal model of acute seizures [16]. Betaine (Sigma-Aldrich, B2629) was dissolved in normal saline, and administrated at a dose of 200 mg/kg or 600 mg/kg, i.p., to mice for 15 days before PTZ injection in reference to a previous study."
item3247 "BALB/c-Nu nude mice (female, 5 weeks old) underwent UVA+UVB irradiation (Sxientz03-II UV-light cross-linker) 5 times weekly for 8 weeks. The initial dose of UVB was the minimal erythemal dose (MED),approximately 100 mJ/cm2, for the first week,. Then, the dose of UVB was increased by 1 MED per week until 4 weeks, and then 4 MEDs were kept constant for the remaining 4 weeks. The dose of UVA was approximately 10 times that of UVB (approximately 1000 mJ/cm2 for the first week) ."
item3248 .
item3249 .
item3250 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3251 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-7 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3252 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3253 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-7 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3254 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3255 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-7 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3256 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3257 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 °C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-7 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
item3258 "DBA/1 mice were injected intradermally with 100 μg of bovine type II collagen (CII) emulsified in 50 μL of complete Freund's adjuvant at a 1:1 ratio (vol/vol) as primary immunization on day 0. On day 21, the mice were boosted with CII emulsified at a 1:1 ratio in incomplete Freund's adjuvant. The weight, arthritis score, and thickness of the paws were observed and recorded once every 3 days. Starting on day 21, the CIA mice were administered an equal volume of methotrexate (MTX, 1 mg/kg) and METTL3 inhibitor STM2457 (50 mg/kg), which were intraperitoneally injected with twice a week. All mice were sacrificed and specimens were harvested on day 63."
item3259 .
item3260 "Eight-week-old male and female mice were given DOX (5 mg/kg, Cat No. D1515, Sigma-Aldrich) or saline once weekly for 4 weeks via intraperitoneal injection [29]. For experiments with STM2457 (Cat No. SML3360, Sigma-Aldrich), WT mice received STM2457 (50 mg/kg dissolved in 10% DMSO and 90% saline, i. p.) once daily . Cardiac function was evaluated using echocardiography 1 week following the final DOX. Then, heart tissues were collected after echocardiography assessment for biochemical assays."
item3261 "Eight-week-old male and female mice were given DOX (5 mg/kg, Cat No. D1515, Sigma-Aldrich) or saline once weekly for 4 weeks via intraperitoneal injection [29]. For experiments with STM2457 (Cat No. SML3360, Sigma-Aldrich), WT mice received STM2457 (50 mg/kg dissolved in 10% DMSO and 90% saline, i. p.) once daily . Cardiac function was evaluated using echocardiography 1 week following the final DOX. Then, heart tissues were collected after echocardiography assessment for biochemical assays."
item3262 .
item3263 .
item3264 .
item3265 .
item3266 .
item3267 .
item3268 .
item3269 .
item3270 .
item3271 .
item3272 .
item3273 .
item3274 .
item3275 .
item3276 .
item3277 .
item3278 .
item3279 "To increase the accuracy of the experimental results in vivo, we subcutaneously transplanted tumor tissues from HCC patients into BALB/c nude mice and then treated the tumor-bearing mice with lenvatinib to establish a Lenvatinib-resistant PDX mode."
item3280 "Seven-day-old C57BL/6J mice and their lactating mothers were exposed to a 75 % oxygen environment in a chamber for a period of 5 days. Subsequently, the mice were transferred to normal room air (21 % oxygen) at postnatal day 12 (P12) and kept under these conditions for an additional 5 days. At P17, the mice were sacrificed by 10 min following an intraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (10 mg/kg), and their eyes were enucleated and processed for immunostaining or immunoblotting."
item3281 "Female mice (8 weeks old, 20-25 g) on a C57BL/6J background were fasted for 12 h but were allowed to drink water freely. They were then injected intraperitoneally with 50 mg/kg body weight of freshly dissolved STZ in sterile PBS for four consecutive days. Mice were given sterile PBS alone in the same way as an untreated control. The mice's blood glucose levels were assessed two weeks after their most recent treatment. Mice that exhibited glucose levels greater than 200 mg/dL were classified as successful hyperglycemic models and were utilized in subsequent studies. Five months after the final dose of STZ, the mice were euthanized, and the renal tissues were collected for pathological examination to confirm the successful establishment of the model of diabetes nephropathy induced by hyperglycemia. All animal experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at the China Pharmaceutical University. Isoflurane was used to anesthetize mice."
item3282 "Female mice (8 weeks old, 20-25 g) on a C57BL/6J background were fasted for 12 h but were allowed to drink water freely. They were then injected intraperitoneally with 50 mg/kg body weight of freshly dissolved STZ in sterile PBS for four consecutive days. Mice were given sterile PBS alone in the same way as an untreated control. The mice's blood glucose levels were assessed two weeks after their most recent treatment. Mice that exhibited glucose levels greater than 201 mg/dL were classified as successful hyperglycemic models and were utilized in subsequent studies. Five months after the final dose of STZ, the mice were euthanized, and the renal tissues were collected for pathological examination to confirm the successful establishment of the model of diabetes nephropathy induced by hyperglycemia. All animal experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at the China Pharmaceutical University. Isoflurane was used to anesthetize mice."
item3283 "Female mice (8 weeks old, 20-25 g) on a C57BL/6J background were fasted for 12 h but were allowed to drink water freely. They were then injected intraperitoneally with 50 mg/kg body weight of freshly dissolved STZ in sterile PBS for four consecutive days. Mice were given sterile PBS alone in the same way as an untreated control. The mice's blood glucose levels were assessed two weeks after their most recent treatment. Mice that exhibited glucose levels greater than 202 mg/dL were classified as successful hyperglycemic models and were utilized in subsequent studies. Five months after the final dose of STZ, the mice were euthanized, and the renal tissues were collected for pathological examination to confirm the successful establishment of the model of diabetes nephropathy induced by hyperglycemia. All animal experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at the China Pharmaceutical University. Isoflurane was used to anesthetize mice."
item3284 "Rats were anesthetized with 40 mg/kg sodium pentobarbital. A 21G needle was used to puncture discs 7-8 (Co7-8) from the back through the skin of the tail. The length of the needle was predetermined to ensure a puncture depth of approximately 5 mm. After penetrating the annulus, the needle was rotated 360° and held for 30 seconds to injure the annulus. The sham group underwent the same procedure but without puncture injury to the caudal disc."
item3285 "Rats were anesthetized with 40 mg/kg sodium pentobarbital. A 21G needle was used to puncture discs 7-8 (Co7-8) from the back through the skin of the tail. The length of the needle was predetermined to ensure a puncture depth of approximately 5 mm. After penetrating the annulus, the needle was rotated 360° and held for 31 seconds to injure the annulus. The sham group underwent the same procedure but without puncture injury to the caudal disc."
item3286 "After one week of acclimatization, the nude mice were randomly divided into 5 groups (n = 3): Control, vehicle + oe-NC, vehicle + oe-CHOP, Regorafenib + oe-NC, and Regorafenib + oe-CHOP. The Control group was injected with untreated SK-Hep-1 cells. The vehicle + oe-NC group and Regorafenib + oe-NC group were injected with SK-Hep-1 cells transfected with oe-NC. vehicle + oe-CHOP group and Regorafenib + oe-CHOP group were injected with SK-Hep-1 cells transfected with oe-CHOP."
item3287 "Under a specific pathogen-free environment with a 12 h light/dark cycle, BALB/C male nude mice (Slaike Jingda Laboratory, Hunan, China) were randomly divided into two groups. Meanwhile, all mice had free access to fresh water and feed. For the COAD model, 5×106 DLD1 cells sh-NC or sh-CDCA7 were suspended in 100 μL PBS and subcutaneously injected into mice(n=5 for each group). During this period, the volume of tumors was continuously examined every week to generate the tumor growth curve. After injection for five weeks, all mice were euthanized, and tumors were subjected to image, weight, and western blot analysis. Meanwhile, Immunohistochemical (IHC) staining was used for the detection of CDCA7-positive expression in dissected tumor tissues."
item3288 .
item3289 .
item3290 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/2 days off treatment regimen was applied."
item3291 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/3 days off treatment regimen was applied."
item3292 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/4 days off treatment regimen was applied."
item3293 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/5 days off treatment regimen was applied."
item3294 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/6 days off treatment regimen was applied."
item3295 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/7 days off treatment regimen was applied."
item3296 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/8 days off treatment regimen was applied."
item3297 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/9 days off treatment regimen was applied."
item3298 "For the subcutaneous injection model, 3 × 106 MHCC97L cells were washed with PBS thrice and resuspended in 100 μL 1:1 Matrixgel and DMEM-HG. HDTVi was performed as previously described.44 Plasmids were extracted using an EndoFree Plasmid extraction kit (QIAGEN). Plasmid sequence was confirmed using Sanger sequencing. A 2 mL mixture of 20 μg sgTp53 plasmids, 10 μg Myc overexpression plasmids, and 2 μg SB13 plasmids was dissolved in saline of 10% mice body weight and was tail-vein injected into C57BL/6 mice weighing at least 25 g. Plasmids were co-injected in the lateral tail vein to induce spontaneous HCC formation. Three weeks after HDTVi, mice were randomly subjected to vehicle or STM2457 treatment. STM2457 (DC chemicals) was dissolved in 20% (w/v) 2-hydroxyproply beta-cyclodextrin vehicle (Sigma, H107) and injected intraperitoneally. Sorafenib powder (Selleckchem) was dissolved in distilled water and administrated via oral gavage. A 5 days on/9 days off treatment regimen was applied."
item3299 "ShRNA was purchased from GenePharma (Shanghai, China), male C57BL/6 mice (4 weeks, n = 5 per group) and male BALB/c nude mice (4 weeks, n = 5 per group) were obtained from Shulaibao Biotech (Wuhan, China). The cells were pre-treated and separated into knockdown and control groups. The nude mice were subcutaneously injected with 100 μL (containing 107 cells) into the left axillary fold. The status of the nude mice was observed and recorded daily after injection. The minimum width (W) and maximum length (L) of the tumor were measured with calipers per four days."
item3300 .
item3301 "Female 8-week-old Wistar rats (weighed 200 ± 20 g) were housed under a 12-h light/dark cycle with temperature and humidity (23 ± 1°C and 52 ± 2%) maintained, and they had free access to food and tap water. After 1 week of adaptive feeding, rats were anesthetized by intraperitoneal injection of 40 mg/kg of sodium thiopental. A rat model of OA was established according to the reference.32 Iodoacetate causes joint pathology via the inhibition of glycolysis, thereby targeting the avascular cartilage and causing chondrocyte death.33 With the left leg flexed at a 90° angle at the knee, 2 mg of monosodium iodoacetate (Sigma-Aldrich, MO, USA) was injected through the patellar ligament between the tibia and the femur using a 26GX3/8 needle at a volume of 25 μL. Control rats received an intra-articular injection of sterile saline (25 μL) alone. After recovery, the animals were returned to cages. Seven days after injection, the cartilages in the control and OA groups were collected for gene expression analysis."
item3302 .
item3303 "Six-week-old male BABL/c mice were purchased from Hunan SJA Laboratory Animal Co., Ltd. The mice were initially divided into four groups, each containing 6 mice: Control group, Control+S. boulardii group, Model group, and Model+S. boulardii group. With reference to the previous OVA method [27,28], the animal model of asthma was established. Mice in Control+S. boulardii and Model+S. boulardii groups were administered 300 μL/day of 1 × 107 CFU/d S. boulardiithrough oral gavage, starting from 6 days prior to the first OVA sensitization and continuing until day 21 [12,29]. The mice in Control and Control+S. boulardiigroups were injected with an equivalent volume of PBS. After the final OVA injection, the mice were challenged with 10 μL of 5 % OVA via intranasal inhalation for one week (day 15 to day 21), with 10 μL introduced into each nostril. The mice in Control and Control+S. boulardii groups were injected with an equivalent volume of PBS. Mice were sacrificed with pentobarbital sodium (150 mg/kg, intraperitoneal injection). On day 22, bronchoalveolar lavage fluid (BALF), intestinal tissue, and lung tissue were collected from all mice."
item3304 .
item3305 .
item3306 "Two-to-three-week-old male C57BL/6 mice were randomly divided into five groups based on their body weight: control (deionised water), solvent control (0.5 % CMC Na), T-2 (200 ng/g T-2 toxin), treatment (administered a specially formulated methionine diet one month after exposure), and prevention groups (fed a specially formulated methionine diet). Each group consisted of six mice that received treatment for 8 weeks. The T-2 toxin gavage solution was prepared using 0.5 % CMC Na solution. The standard diet contained 0.57 % methionine, whereas the specially formulated diet contained 1.2 % methionine."
item3307 Male and female mice were group-housed in polycarbonate cages with corncob bedding; they were maintained in a humidity-and temperature-controlled vivarium (20-22°C) on a 12/12 h light/dark schedule. Animals had access ad libitum to food and water except during behavioral testing. All experiments requiring wild-type mice will use C57BL/6J mice from Jackson Laboratories (Stock No: 000664).
item3308 .
item3309 .
item3310 "2-month-old mice were given doses of tamoxifen at 40 mg/kg body weight/ day for 5 consecutive days by intraperitoneal injection. Littermate mice with wild-type levels of Ythdf1, either expressing Cre recombinase only or containing the Ythdf1 flox/flox-only, were used as controls (Ctrl). All mice received tamoxifen injections. All in vivo experiments and analyses were performed 1 month after the first tamoxifen injection."
item3311 .
item3312 "For the subcutaneous implantation model, 5 × 106 cells (n = 6 per group) were subcutaneously injected into the flank regions of 4-5 weeks female BALB/c nude mice. Tumor volume was calculated as 0.5 × W2 × L (where W and L represent a tumor's width and length, respectively). After xenografts were generated, DMSOand SB431542 (10 mg/kg, #301836-41-9, SANTA CRUZ BIOTECHNOLOGY), or ENOblock (#1177827-73-4, 8 mg/kg, MCE) were administered daily. All animal experiments were approved by The Affiliated Hospital of Qingdao University Committee on Animal Care."
item3313 "For the subcutaneous implantation model, 5 × 106 cells (n = 6 per group) were subcutaneously injected into the flank regions of 4-5 weeks female BALB/c nude mice. Tumor volume was calculated as 0.5 × W2 × L (where W and L represent a tumor's width and length, respectively). After xenografts were generated, DMSOand SB431542 (10 mg/kg, #301836-41-9, SANTA CRUZ BIOTECHNOLOGY), or ENOblock (#1177827-73-4, 9 mg/kg, MCE) were administered daily. All animal experiments were approved by The Affiliated Hospital of Qingdao University Committee on Animal Care."
item3314 "For the subcutaneous implantation model, 5 × 106 cells (n = 6 per group) were subcutaneously injected into the flank regions of 4-5 weeks female BALB/c nude mice. Tumor volume was calculated as 0.5 × W2 × L (where W and L represent a tumor's width and length, respectively). After xenografts were generated, DMSOand SB431542 (10 mg/kg, #301836-41-9, SANTA CRUZ BIOTECHNOLOGY), or ENOblock (#1177827-73-4, 10 mg/kg, MCE) were administered daily. All animal experiments were approved by The Affiliated Hospital of Qingdao University Committee on Animal Care."
item3315 "We also used surgery to cause spine instability in rats to observe IVD changes under abnormal stress (Figure 1f). X-ray scans 6 months after surgery showed that the lumbar vertebral sequence line was continuous, with no stenosis in various intervertebral foramen in the ctrl group."
item3316 "We also used surgery to cause spine instability in rats to observe IVD changes under abnormal stress (Figure 1f). X-ray scans 6 months after surgery showed that the lumbar vertebral sequence line was continuous, with no stenosis in various intervertebral foramen in the ctrl group."
item3317 .
item3318 .
item3319 "HCC cells stably transfected with a firefly luciferase expression vector (1 × 106 cells 30 μL) were injected into the left liver lobe of 5-week-old male BALB/c nude mice. Luciferase bioluminescence in each mouse was measured at week 6 after injection. Mice in all the groups were sacrificed 8 weeks after injection. ii) For the lung metastasis model, we slowly injected HCC cells (1 × 106cells 100 μL) into 5-week-old male BALB/c nude mice via the tail vein. Six weeks after injection, luciferase bioluminescence was measured, and the mice were then sacrificed. iii) As described in the previous study, 6-8-week-old male wild-type C57BL/6J mice were used for hydrodynamic tail vein injection (HTVi). The pT3-EF1alpha-myr-AKT-HA (human), pT3-EF1alpha-N90-beta-catenin (human), and pT3-EF1alpha-ITIH1 (human) plasmids were constructed by using the pT3-EF1alpha vector. The pooled pT3 plasmids and the Sleeping Beauty transposon plasmid (pCMV-SB) were mixed at a ratio of 25:1 and 20 μg of each pT3 plasmid was used for each mouse."
item3320 "For xenograft establishment, six-week-old female athymic nude mice (n = 6 per group) were used, following ethical guidelines approved by the Institutional Animal Care and Use Committee. Each mouse received subcutaneous injections in the flank region with 1 × 10^6 cells suspended in 100 μL of serum-free medium, either sh-METTL3-transfected A549 cells or sh-NC-transfected controls. Tumour growth was monitored bi-dimensionally every week using callipers, with tumour volume calculated using the formula (length × width^2)/2. After 4 weeks, the mice were anaesthetised and euthanized by intraperitoneal injection of 3% pentobarbital sodium (100 mg/kg). The tumours were weighted and used for HE and Immunohistochemistry staining."
item3321 "For xenograft establishment, six-week-old female athymic nude mice (n = 6 per group) were used, following ethical guidelines approved by the Institutional Animal Care and Use Committee. Each mouse received subcutaneous injections in the flank region with 1 × 10^6 cells suspended in 100 μL of serum-free medium, either sh-METTL3-transfected A549 cells or sh-NC-transfected controls. Tumour growth was monitored bi-dimensionally every week using callipers, with tumour volume calculated using the formula (length × width^2)/2. After 4 weeks, the mice were anaesthetised and euthanized by intraperitoneal injection of 3% pentobarbital sodium (100 mg/kg). The tumours were weighted and used for HE and Immunohistochemistry staining."
item3322 "BALB/c mice (female) and C57BL/6 mice (male) (8-12 weeks-old, 20-22 g) were obtained from the Shanghai Laboratory Animal Center (Shanghai, China). Pregnancy day 0.5 (E0.5) was determined by measuring the vaginal plug. The m6A inhibitor, 3-deazaadenosine (40 μL at a concentration of 10 mg/kg dissolved in physiological saline; Sigma-Aldrich, St. Louis, MO, USA) was injected into the uterine cavity using a small catheter (Instech Laboratories, Plymouth Meeting, PA, USA) at E6.5, E5.5, and E4.5, respectively, whereas the control group was injected with an equal volume of saline solution. Mice were sacrificed at E10.5, embryo resorption was measured and photographed, and the placentas were collected. The resorbed embryos were small (<20 % of the average size), appeared dark with unclear boundaries between the fetus and the placenta, and showed necrosis."
item3323 .
item3324 .
item3325 .
item3326 "The mice were kept under standard conditions and provided with care in accordance with established protocols. For the experiment, 1 × 106 DU145 cells with reduced RBM15B expression and corresponding control cells were injected subcutaneously into the right flank of each nude mouse. Tumor length (L) and width (W) were measured weekly, and tumor volume was calculated using the formula: Volume = (W2 × L) / 2. Four weeks following the injections, mice were euthanized by intraperitoneal administration of sodium pentobarbital at a dose of 150 mg/kg. Tumors were then surgically removed and weighed."
item3327 "The mice were kept under standard conditions and provided with care in accordance with established protocols. For the experiment, 1 × 106 DU145 cells with reduced RBM15B expression and corresponding control cells were injected subcutaneously into the right flank of each nude mouse. Tumor length (L) and width (W) were measured weekly, and tumor volume was calculated using the formula: Volume = (W2 × L) / 2. Four weeks following the injections, mice were euthanized by intraperitoneal administration of sodium pentobarbital at a dose of 151 mg/kg. Tumors were then surgically removed and weighed."
item3328 "Female C57BL/6 mice, aged eight weeks, were selected for the study. The mice were divided into three groups with six mice per group. After intramuscular anesthesia (xylazine 10 mg/kg, ketamine 100 mg/kg), two groups underwent bilateral ovariectomy (OVX), while the remaining group had a sham operation. Ovariectomized mice were administered weekly intratibial injections of Mettl14 overexpression adenovirus containing at a concentration of 109 plaque-forming unit per injection, for a duration of 8 weeks. The mice were humanely euthanized one week after the final injection, and their tibia were gathered for further analysis. This study was approved by the ethics committee of Tongji University (NO: TJBH00823101). All the experimental methods were carried out in accordance with the approved guidelines. All experimental procedures involving mice were carried out in strict accordance with the recommendations in the ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments)."
item3329 "Female C57BL/6 mice, aged eight weeks, were selected for the study. The mice were divided into three groups with six mice per group. After intramuscular anesthesia (xylazine 10 mg/kg, ketamine 100 mg/kg), two groups underwent bilateral ovariectomy (OVX), while the remaining group had a sham operation. Ovariectomized mice were administered weekly intratibial injections of Mettl14 overexpression adenovirus containing at a concentration of 109 plaque-forming unit per injection, for a duration of 8 weeks. The mice were humanely euthanized one week after the final injection, and their tibia were gathered for further analysis. This study was approved by the ethics committee of Tongji University (NO: TJBH00823101). All the experimental methods were carried out in accordance with the approved guidelines. All experimental procedures involving mice were carried out in strict accordance with the recommendations in the ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments)."
item3330 .
item3331 .
item3332 "Female BALB/C-nude mice (5 to 6 weeks old) were used for in vivo subcutaneous xenograft tumor and liver metastasis models. MKN-45 cells (6 × 106) transfected with Control or sh-hnRNPA2B1 lentiviruses were injected subcutaneously into the right flank of nude mice. After 1 week of inoculation, mice were randomly divided into four groups (4 mice/group) and treated as follow: 1) Control with PBS treatment; 2) Control with CDDP treatment; 3) sh-hnRNPA2B1 with PBS treatment; 4) sh-hnRNPA2B1 with CDDP treatment. CDDP was delivered 3 mg/kg per 3 days, intraperitoneally. The effect was determined by tumor volume and tumor weight. The volume(mm3) = length × width2 × 0.5.For the liver metastasis model, mice were randomly divided into 2 groups (n = 4 per group) and 2 × 106 MKN-45 cells were injected into the spleen of the BALB/c nude mice. After 2 months, the mice were sacrificed to harvest liver/tumor tissues for analysis. The animal experiments were approved by the Ethics Committee of Shanghai Sixth People's Hospital."
item3333 .
item3334 "Rats were anesthetized with isoflurane (1.5%-2.5%), and a lateral thigh incision was made. The left sciatic nerve and its three peripheral branches were isolated after sciatic nerve resection. A 5-0 suture was used to ligate the common peroneal and tibial nerves. Then, distal transverse incisions were made at the ligation. All surgical procedures were completed within 20 min, with special care taken to avoid damaging the sural nerve. The rats in the sham group underwent the same sciatic nerve exposure operation as the SNI group except for nerve ligation."
item3335 "Rats were anesthetized with isoflurane (1.5%-2.5%), and a lateral thigh incision was made. The left sciatic nerve and its three peripheral branches were isolated after sciatic nerve resection. A 5-0 suture was used to ligate the common peroneal and tibial nerves. Then, distal transverse incisions were made at the ligation. All surgical procedures were completed within 20 min, with special care taken to avoid damaging the sural nerve. The rats in the sham group underwent the same sciatic nerve exposure operation as the SNI group except for nerve ligation."
item3336 "A total of six female BALB/c nude mice (4-week-old) were evenly divided to sh-NC group and sh-circ group. Then, 2 × 107 HeLa cells, transfected with either sh-NC or sh-circ, were subcutaneously injected into the mice. The tumor volume was observed and recorded weekly using a vernier caliper. After 4 weeks, the mice were sacrificed, and the tumors were dissected. The tumor from each mouse was imaged, and their weights were measured. This experiment was approved by the Ethics Committee of Wuhan Third Hospital."
item3337 "Mice were injected with empty vector (pcDNA3) or Flag-tagged METTL3 expression vector (pFlag-METTL3) at 2 days after UUO or 4 days after UIRI, respectively. The expression of transgene was validated by Western blotting or immunostaining for Flag-tagged METTL3 fusion protein."
item3338 "Mice were injected with empty vector (pcDNA3) or Flag-tagged METTL3 expression vector (pFlag-METTL3) at 2 days after UUO or 4 days after UIRI, respectively. The expression of transgene was validated by Western blotting or immunostaining for Flag-tagged METTL3 fusion protein."
item3339 .
item3340 .
item3341 "After anesthesia with ketamine-xylazine(intraperitoneal injection, 60 and 5 mg/kg, respectively), the rats were operated to remove the bilateral ovaries. For the rats in the Sham group, only the fat around the ovaries was surgically removed. Four weeks after the operation, the animals were orally treated with ICA (250 mg/kg) daily for 10 weeks ."
item3342 "C57BL/6 mice were anesthetized intraperitoneally with 1% sodium pentobarbital (100 mg/kg). The mouse was placed on a thermostatic blanket to maintain the rectal temperature at 37.0°C± 0.5°C during surgery. The left common carotid artery (CCA), external carotid artery (ECA), and internal carotid artery (ICA) were exposed through a midline incision in the cervico abdominal region. The CCA was ligated distally with surgical nylon monofilament."
item3343 "C57BL/6 mice were anesthetized intraperitoneally with 1% sodium pentobarbital (100 mg/kg). The mouse was placed on a thermostatic blanket to maintain the rectal temperature at 37.0°C± 0.5°C during surgery. The left common carotid artery (CCA), external carotid artery (ECA), and internal carotid artery (ICA) were exposed through a midline incision in the cervico abdominal region. The CCA was ligated distally with surgical nylon monofilament."
item3344 "Sexually mature mice (older than 8 weeks of age) were mated and the vaginal plugs were confirmed the following morning. The day that a vaginal plug was observed was considered 0.5 days post coitum (0.5 dpc) or embryonic day 0.5 (E0.5). The uterus was cut along the side bearing the embryos and then cut open on the side opposite the placenta. Forceps were used to gently separate the uterus and the maternal surface of the placenta, and then the fetal surface of the placenta and the amniotic sac were separated. Dissected placentas and fetuses were photographed and weighted individually."
item3345 "Sexually mature mice (older than 8 weeks of age) were mated and the vaginal plugs were confirmed the following morning. The day that a vaginal plug was observed was considered 0.5 days post coitum (0.5 dpc) or embryonic day 0.5 (E0.5). The uterus was cut along the side bearing the embryos and then cut open on the side opposite the placenta. Forceps were used to gently separate the uterus and the maternal surface of the placenta, and then the fetal surface of the placenta and the amniotic sac were separated. Dissected placentas and fetuses were photographed and weighted individually."
item3346 "Sexually mature mice (older than 8 weeks of age) were mated and the vaginal plugs were confirmed the following morning. The day that a vaginal plug was observed was considered 0.5 days post coitum (0.5 dpc) or embryonic day 0.5 (E0.5). The uterus was cut along the side bearing the embryos and then cut open on the side opposite the placenta. Forceps were used to gently separate the uterus and the maternal surface of the placenta, and then the fetal surface of the placenta and the amniotic sac were separated. Dissected placentas and fetuses were photographed and weighted individually."
item3347 "Sexually mature mice (older than 8 weeks of age) were mated and the vaginal plugs were confirmed the following morning. The day that a vaginal plug was observed was considered 0.5 days post coitum (0.5 dpc) or embryonic day 0.5 (E0.5). The uterus was cut along the side bearing the embryos and then cut open on the side opposite the placenta. Forceps were used to gently separate the uterus and the maternal surface of the placenta, and then the fetal surface of the placenta and the amniotic sac were separated. Dissected placentas and fetuses were photographed and weighted individually."
item3348 "PCOS mice model was induced by the continuous subcutaneous injection of dehydroepiandrosterone (DHEA, GAOYUANbio, China) with a concentration of 60 mg/kg every day for 3 weeks. Meanwhile, mice in control group were subcutaneously injected with an equal volume of sesame oil. After three weeks, PCOS mice were divided into sh-NC and sh-METTL3 groups at random (n = 10). The ovary of mice in sh-NC group or sh-METTL3 group were subcutaneously injected with sh-NC or sh-METTL3 lentivirus at the concentration of 5 times; 108 PFU/mL synthesized by Shanghai GenePharma, Ltd. The estrus cycle, ovarian weight and follicular development were recorded 14 days following lentivirus infection. The ovary samples were collected for analysis."
item3349 "Male Wistar rats were injected at approximately 1.5 cm distal to the base of the tail with emulsion consisting of collagen (2 mg/ml, Chondrex, USA) and complete Freund's adjuvant(CFA) (5 mg/ml, Chondrex, USA) (at the ratio of 1:1) in a volume of 200 μl per rat. 7 days later, a booster immunization was performed by injecting the same concentration of emulsion as the first immunization in a volume of 100 μl per rat. Subsequently, twice intra-articular injection of adenovirus (Control vector or shFTO, 2 × 108 PFU, 50 μl) (Genechem, Shanghai, China) were prescribed to rats at 8 and 15 days after the primary immunization. Arthritis progression was monitored every three days according to the previously described scoring system ranging from 0 to 4 (0, normal; 1, swelling or redness of paw or a single digit; 2, 2 joints involved; 3, 3 joints involved; 4, severe arthritis of the entire paw and digits). The arthritic score was independently calculated in a blinded manner and defined as the average score of hind paws."
item3350 "Male Wistar rats were injected at approximately 1.5 cm distal to the base of the tail with emulsion consisting of collagen (2 mg/ml, Chondrex, USA) and complete Freund's adjuvant(CFA) (5 mg/ml, Chondrex, USA) (at the ratio of 1:1) in a volume of 200 μl per rat. 7 days later, a booster immunization was performed by injecting the same concentration of emulsion as the first immunization in a volume of 100 μl per rat. Subsequently, twice intra-articular injection of adenovirus (Control vector or shFTO, 2 × 108 PFU, 50 μl) (Genechem, Shanghai, China) were prescribed to rats at 8 and 15 days after the primary immunization. Arthritis progression was monitored every three days according to the previously described scoring system ranging from 0 to 4 (0, normal; 1, swelling or redness of paw or a single digit; 2, 2 joints involved; 3, 3 joints involved; 4, severe arthritis of the entire paw and digits). The arthritic score was independently calculated in a blinded manner and defined as the average score of hind paws."
item3351 .
item3352 .
item3353 .
item3354 .
item3355 .
item3356 .
item3357 .
item3358 "36 male C57BL/6J mice (20-22 g, 2-month-old) were purchased from SPF (Beijing) biotechnology co., Ltd (Beijing, China) and maintained in the specific pathogen-free (SPF) animal laboratory with a 12/12 h light/dark cycle with free access to food and water. The mice were randomly assigned into six groups (n = 6 per group): (1) sham-operated group (Sham), (2) MCAO 6 h, (3) MCAO 12 h, (4) MCAO 1 d, (5) MCAO 3 d, and (6) MCAO 7 d."
item3359 "36 male C57BL/6J mice (20-22 g, 2-month-old) were purchased from SPF (Beijing) biotechnology co., Ltd (Beijing, China) and maintained in the specific pathogen-free (SPF) animal laboratory with a 12/12 h light/dark cycle with free access to food and water. The mice were randomly assigned into six groups (n = 6 per group): (1) sham-operated group (Sham), (2) MCAO 6 h, (3) MCAO 12 h, (4) MCAO 1 d, (5) MCAO 3 d, and (6) MCAO 7 d."
item3360 "36 male C57BL/6J mice (20-22 g, 2-month-old) were purchased from SPF (Beijing) biotechnology co., Ltd (Beijing, China) and maintained in the specific pathogen-free (SPF) animal laboratory with a 12/12 h light/dark cycle with free access to food and water. The mice were randomly assigned into six groups (n = 6 per group): (1) sham-operated group (Sham), (2) MCAO 6 h, (3) MCAO 12 h, (4) MCAO 1 d, (5) MCAO 3 d, and (6) MCAO 7 d."
item3361 "36 male C57BL/6J mice (20-22 g, 2-month-old) were purchased from SPF (Beijing) biotechnology co., Ltd (Beijing, China) and maintained in the specific pathogen-free (SPF) animal laboratory with a 12/12 h light/dark cycle with free access to food and water. The mice were randomly assigned into six groups (n = 6 per group): (1) sham-operated group (Sham), (2) MCAO 6 h, (3) MCAO 12 h, (4) MCAO 1 d, (5) MCAO 3 d, and (6) MCAO 7 d."
item3362 "36 male C57BL/6J mice (20-22 g, 2-month-old) were purchased from SPF (Beijing) biotechnology co., Ltd (Beijing, China) and maintained in the specific pathogen-free (SPF) animal laboratory with a 12/12 h light/dark cycle with free access to food and water. The mice were randomly assigned into six groups (n = 6 per group): (1) sham-operated group (Sham), (2) MCAO 6 h, (3) MCAO 12 h, (4) MCAO 1 d, (5) MCAO 3 d, and (6) MCAO 7 d."
item3363 .
item3364 .
item3365 .
item3366 "Eight 4-week-old female nude mice were purchased from Gempharmatech Co. Ltd. and were randomly divided into two groups. In order to establish the in vivo lymph node metastasis model, 3 × 106 SiHa-lv-shFTO or SiHa-lv-shcon cells were slowly injected into the foot pads that containing abundant lymph nodes. After feeding for 20 weeks, we euthanized the nude mice. Lymph nodes in the groin area are dissected and embedded in paraffin for hematoxylin-eosin staining. Our animal experiment research was carried out strictly in accordance with the ""Guidelines for the Welfare of Experimental Tumor Animals""."
item3367 "Mice were randomly allocated into three groups (n = 5/group): the negative control (NEG) group, the DSS-induced colitis (DSS) group, and the hucMSC-Ex-treated colitis (hucMSC-Ex) group. Mice in the NEG group were fed autoclaved water during the experiment, while mice in the DSS and hucMSC-Ex groups were fed autoclaved water containing 3% DSS (MP Biomedicals, USA). A total of 1 mg of hucMSC-Ex was administered by caudal vein to mice in the hucMSC-Ex group on the 3rd, 6th, and 9th day of modeling, while mice in other groups were injected with PBS. All mice were sacrificed when severe hematochezia and weight loss were observed in the DSS group. The disease progression of mice in the different groups was evaluated by weight change, DAI score, colorectal length, and weight ratio. After the mice were sacrificed, the general view of the spleen and colorectum of the mice was observed. The colorectal mucosa and spleen tissues of the mice in each group were separated, and RNA and protein of the tissues were extracted for subsequent experiments."
item3368 "Mice were randomly allocated into three groups (n = 5/group): the negative control (NEG) group, the DSS-induced colitis (DSS) group, and the hucMSC-Ex-treated colitis (hucMSC-Ex) group. Mice in the NEG group were fed autoclaved water during the experiment, while mice in the DSS and hucMSC-Ex groups were fed autoclaved water containing 3% DSS (MP Biomedicals, USA). A total of 1 mg of hucMSC-Ex was administered by caudal vein to mice in the hucMSC-Ex group on the 3rd, 6th, and 9th day of modeling, while mice in other groups were injected with PBS. All mice were sacrificed when severe hematochezia and weight loss were observed in the DSS group. The disease progression of mice in the different groups was evaluated by weight change, DAI score, colorectal length, and weight ratio. After the mice were sacrificed, the general view of the spleen and colorectum of the mice was observed. The colorectal mucosa and spleen tissues of the mice in each group were separated, and RNA and protein of the tissues were extracted for subsequent experiments."
item3369 "When the tumors in the CDX and PDX mice grew to about 80-150 mm3, forty tumor-bearing mice were randomly divided into four groups (ten mice in each group) according to the size of the transplanted tumor and the principle of consistency between groups. The four groups were: as follows: solvent control group, Len (3 mg kg-1) administration group, Res (0.375 mg kg-1) administration group, and Len (3 mg kg-1) plus Res (0.375 mg kg-1) administration group. The administration mode of all groups was intragastric and the volume was 0.2 ml/20 g. Mouse body weight and xenograft tumor volume were measured every other day. Tumor volume was measured weekly and calculated as V = (length × width2)/2. Three weeks after the onset of treatment, the tumors were excised and weighed. After the experiment, the heart, liver, spleen, lung and kidney tissues of the mice were completely removed and weighed. Then the organ index was calculated as organ index = weight of each organ/body weight × 100 %."
item3370 "When the tumors in the CDX and PDX mice grew to about 80-150 mm3, forty tumor-bearing mice were randomly divided into four groups (ten mice in each group) according to the size of the transplanted tumor and the principle of consistency between groups. The four groups were: as follows: solvent control group, Len (3 mg kg-1) administration group, Res (0.375 mg kg-1) administration group, and Len (3 mg kg-1) plus Res (0.375 mg kg-1) administration group. The administration mode of all groups was intragastric and the volume was 0.2 ml/20 g. Mouse body weight and xenograft tumor volume were measured every other day. Tumor volume was measured weekly and calculated as V = (length × width2)/2. Three weeks after the onset of treatment, the tumors were excised and weighed. After the experiment, the heart, liver, spleen, lung and kidney tissues of the mice were completely removed and weighed. Then the organ index was calculated as organ index = weight of each organ/body weight × 100 %."
item3371 "The adenovirus-mediated silencing METTL14 (ad-sh-METTL14) or negative control vectors were designed and packaged by GenePharma (Shanghai, China). All mice (18 mice in each group) were euthanized at 20 weeks of age by injection of pentobarbital sodium (250 mg/kg)."
item3372 "The adenovirus-mediated silencing METTL14 (ad-sh-METTL14) or negative control vectors were designed and packaged by GenePharma (Shanghai, China). All mice (18 mice in each group) were euthanized at 20 weeks of age by injection of pentobarbital sodium (250 mg/kg)."
item3373 Human A549 cells and Vero cells.
item3374 "The mice were randomly allocated to four groups, with the specific number of mice detailed in each experiment. Subsequently, cells were injected into the abdominal mammary glands of the mice (2 × 106 cells in 100 μL) and allowed to grow until tumor formation occurred within 16 days. The tumor volume at the time of sacrifice was calculated using the formula, Volume = 0.5 × [(largest diameter) × (smallest diameter)^2]. The study protocol was approved by the Animal Experiments Committee of Chosun University (CIACUC 2020-S0022)."
item3375 "Under isoflurane anesthesia, bipolar leads were inserted into the right atrial (RA) appendage and connected to pacemakers. Following a 24-h recovery, the atrial pacemaker was programmed to pace the RA appendage at atrial tachycardia pacing for 2 weeks."
item3376 .
item3377 "1 × 105 treated cells were subcutaneously injected into mice with normal drinking water. When the volume of subcutaneous tumors was about 150 mm3, the mice were kept with drinking water containing 1 mg/mL of Dox. After 3 weeks, the xenograft tumors were collected and used for follow-up experiments."
item3378 "1 × 105 treated cells were subcutaneously injected into mice with normal drinking water. When the volume of subcutaneous tumors was about 150 mm3, the mice were kept with drinking water containing 1 mg/mL of Dox. After 3 weeks, the xenograft tumors were collected and used for follow-up experiments."
item3379 "To assess mortality rates, mice were intraperitoneally administered LPS (30 mg/kg), with mdivi (3 mg/kg) given intraperitoneally 1 h before LPS challenge and then continued for 3 consecutive days. The 72-h mortality was subsequently recorded. For evaluating heart injury, mice received an injection of LPS (10 mg/kg), with songorine (10 or 50 mg/kg) administered 1 h before and 12 h after LPS treatment. Mice were euthanized 24 h later for heart collection."
item3380 "To assess mortality rates, mice were intraperitoneally administered LPS (30 mg/kg), with mdivi (3 mg/kg) given intraperitoneally 1 h before LPS challenge and then continued for 3 consecutive days. The 72-h mortality was subsequently recorded. For evaluating heart injury, mice received an injection of LPS (10 mg/kg), with songorine (10 or 50 mg/kg) administered 1 h before and 12 h after LPS treatment. Mice were euthanized 24 h later for heart collection."
item3381 .
item3382 .
item3383 .
item3384 .
item3385 "In the first in vivo experiment focused on investigating the role of METTL14 in MDS cell proliferation in vivo, the MDS-L-luc cells were transduced with the Dox-inducible shMETTL14 (shMETTL14_Tet-on). After treatment with 2 μg/mL puromycin for a duration of 4 days, a total of 2.5 × 106 selected cells were injected via the tail vein into irradiated female NCG-M mice aged 8-10 weeks (GemPharmatech, China) to establish cell line-derived xenograft (CDX) models. On day 14 post transplantation, 20 mice were randomly assigned to two groups and treated with either Dox or vehicle. A total of 2 mg Dox was dissolved in water and administered by gastric lavage once a day. Five mice from each group underwent in vivo chemiluminescence imaging on day 14, 21, and 28 after receiving intraperitoneal injection of luciferin (Promega, USA). Their overall survival (OS) was also observed and documented. The remaining five mice from each group were utilized to assess the proportions of human CD45+cells in bone marrow and peripheral blood on day 28 through flow cytometry analysis."
item3386 "In the first in vivo experiment focused on investigating the role of METTL14 in MDS cell proliferation in vivo, the MDS-L-luc cells were transduced with the Dox-inducible shMETTL14 (shMETTL14_Tet-on). After treatment with 2 μg/mL puromycin for a duration of 4 days, a total of 2.5 × 106 selected cells were injected via the tail vein into irradiated female NCG-M mice aged 8-10 weeks (GemPharmatech, China) to establish cell line-derived xenograft (CDX) models. On day 14 post transplantation, 20 mice were randomly assigned to two groups and treated with either Dox or vehicle. A total of 2 mg Dox was dissolved in water and administered by gastric lavage once a day. Five mice from each group underwent in vivo chemiluminescence imaging on day 14, 21, and 28 after receiving intraperitoneal injection of luciferin (Promega, USA). Their overall survival (OS) was also observed and documented. The remaining five mice from each group were utilized to assess the proportions of human CD45+cells in bone marrow and peripheral blood on day 28 through flow cytometry analysis."
item3387 "The mice underwent sevoflurane inhalation anesthesia (5% induction and 2% maintenance with spontaneous respiration in 50% O2; Baxter), and a neck incision was made to expose the left common carotid artery along with the internal and external carotid arteries through surgical means. A nylon monofilament coated with silicone rubber (602256PK10, Doccol Corporation) was introduced through an incision in the left external carotid artery. Subsequently, guided through the bifurcation into the internal carotid artery, the monofilament was advanced by approximately 11 mm to induce 90-min occlusion of the middle cerebral artery, followed by a 24-h reperfusion period. Mice in the sham-operated group underwent a matching surgical process, excluding the embolization stage."
item3388 "Twenty nude mice were used to establish the transfer model. KLHL5 or normal SGC-7901 cells were knocked out using luciferase labeling (Genechem, China) and injected through the tail vein to construct tumor models. After 3 weeks, the tumor growth in mice was observed by small animal imaging technology. After the mice were euthanized, lung tissue was taken to observe tumor metastasis. In addition, eight nude mice were subcutaneously implanted with high/low KLHL5-expressing mass tissues from human subjects to evaluate the effect of KLHL5 on xenograft tumor proliferation."
item3389 .
item3390 "Mice were housed in a specific pathogen-free animal facility with a 12-h light-dark cycle. Lentivirus-infected HUCCT1 or HCCC-9810 cells (4 × 106) were suspended in 0.1 mL of phosphate-buffered saline and were subcutaneously injected into the right flank of nude mice. Tumor size was monitored every 4 days for a total of 28 days, and tumor volume was calculated as length × width2/2. After 4 weeks, the mice were euthanized by intraperitoneal injection of 200 mg/kg sodium pentobarbital. Then, tumor tissues were excised and weighed for further analysis."
item3391 "Mice were housed in a specific pathogen-free animal facility with a 12-h light-dark cycle. Lentivirus-infected HUCCT1 or HCCC-9810 cells (4 × 106) were suspended in 0.1 mL of phosphate-buffered saline and were subcutaneously injected into the right flank of nude mice. Tumor size was monitored every 4 days for a total of 28 days, and tumor volume was calculated as length × width2/2. After 4 weeks, the mice were euthanized by intraperitoneal injection of 200 mg/kg sodium pentobarbital. Then, tumor tissues were excised and weighed for further analysis."
item3392 "After anesthesia, mice in the Sham group underwent a sham operation via an incision in the medial capsule of the knee joint (n = 6). Twelve C57BL/6 mice were separated into 2 groups: DMM and DMM + sh-WTAP.Adeno-associated virus 9 containing sh-WTAP (sh-WTAP) and its control virus (WZ Biosciences, Jinan, China) was administered to C57BL/6 J mice (10 μl, 1 × 1012 vg/ml) by intra-articular injection. Three weeks later, the DMM surgery was performed to establish posttraumatic OA mouse models. The knee joints of the mice were collected for subsequent experiments at the eighth week after surgery."
item3393 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
item3394 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
item3395 "Approximately 1 × 106 PCa cells (PC3-shNC, PC3-sh812 cell lines) or 2 × 106 DU145 cells (DU145-shNC, DU145-sh812, DU145-OENC, DU145-OEMETTL3-WT, DU145-OEMETTL3-Mut cell lines) per injection were suspended in a mixture of 100 μL PBS and Matrigel (1:1), and then injected subcutaneously into male BALB/c nude mice aged around 6 ~ 7 weeks old. Tumor volume was monitored at regular intervals and the volume was calculated as (length × width2)/2. Finally, the mice were sacrificed and the tumors were dissected and weighed."
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item3400 "Daily vaginal smears were collected between 4 and 6 PM, and rats showing four consecutive 4-day estrus cycles were chosen for constructing the endometrial injury model. Anesthesia was induced by injecting 3% mebumalnatrium (dose: 1.3 mL/kg) into the lumbar region, followed by a low midline abdominal incision for uterus exposure."
item3401 "Daily vaginal smears were collected between 4 and 6 PM, and rats showing four consecutive 4-day estrus cycles were chosen for constructing the endometrial injury model. Anesthesia was induced by injecting 3% mebumalnatrium (dose: 1.4 mL/kg) into the lumbar region, followed by a low midline abdominal incision for uterus exposure."
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item3503 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3504 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3505 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3506 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3507 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3508 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3509 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3510 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3-/- C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
item3511 .
item3512 .
item3513 .
item3514 .
item3515 .
item3516 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group). Tumor growth was measured by measuring the width (W) and length (L) with calipers, and the volume (V) of the tumor was figured using the criterion V = (W2 × L)/2. At 42 days after injection, the mice were euthanized and tumors were removed and weighed. The tumor samples were further analyzed with IHC and Western blot assay. The animal studies were performed according to the institutional ethics guidelines for animal experiments and approved by the Chongqing Medical University Animal Care and Use Committee."
item3517 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group). Tumor growth was measured by measuring the width (W) and length (L) with calipers, and the volume (V) of the tumor was figured using the criterion V = (W2 × L)/2. At 43 days after injection, the mice were euthanized and tumors were removed and weighed. The tumor samples were further analyzed with IHC and Western blot assay. The animal studies were performed according to the institutional ethics guidelines for animal experiments and approved by the Chongqing Medical University Animal Care and Use Committee."
item3518 "The rats were anaesthetized via strictly aseptic techniques during surgery. The knee joint was opened laterally by dislocating the patellar tendon, and a full thickness osteochondral defect (3-mm in diameter) was created in the trochlear groove of the femur. Then, approximately 107BMSCs were mixed with 1 ml hydrogel matrix (Coring, Cat#354250) and were injected into the defect."
item3519 "The rats were anaesthetized via strictly aseptic techniques during surgery. The knee joint was opened laterally by dislocating the patellar tendon, and a full thickness osteochondral defect (3-mm in diameter) was created in the trochlear groove of the femur. Then, approximately 107BMSCs were mixed with 1 ml hydrogel matrix (Coring, Cat#354250) and were injected into the defect."
item3520 .
item3521 .
item3522 The mice for the xenograft tumour-growth assays and tail-vein assays of cancer metastasis were obtained from Charles River Laboratories. The mice for the PDX model were obtained from and established at the NBRI.
item3523 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
item3524 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
item3525 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
item3526 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
item3527 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
item3528 .
item3529 .
item3530 .
item3531 .
item3532 .
item3533 .
item3534 .
item3535 "An aliquot of the vector at 1011genome equivalents was prepared in 20-30 μL of HBSS and isoflurane anesthesia followed by nasal drops. Mice were randomly divided into five groups as follows: normoxic environment plus control vector group (NOR + NC, n = 20), hypoxic environment plus control vector group (HYP + NC, n = 10), hypoxic environment plus FENDRR TFO2 adenovirus group (HYP + FENDRR TFO2, n = 10), normoxic environment plus FENDRR TFO2 adenovirus group (NOR + FENDRR TFO2, n = 10)."
item3536 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
item3537 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
item3538 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
item3539 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
item3540 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
item3541 .
item3542 .
item3543 .
item3544 .
item3545 .
item3546 .
item3547 "8-week-old male C57BL/6 mice were randomly divided into four groups: Ctrl+AAV-NC group (n = 6), Ctrl+AAV-OT group (n = 6), MIA+AAV-NC group (n = 8), and MIA+ AAV-OT group (n = 8). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. Control mice were given normal saline in the same volume."
item3548 "8-week-old male C57BL/6 mice were randomly divided into four groups: Ctrl+AAV-NC group (n = 6), Ctrl+AAV-OT group (n = 6), MIA+AAV-NC group (n = 8), and MIA+ AAV-OT group (n = 8). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. Control mice were given normal saline in the same volume."
item3549 "Slack Laboratory Animal Co., Ltd. (Shanghai, China) supplied the six-week-old C57BL/6 male mice. Animals were reared at constant temperature (21 ± 1 °C) and maintained in a room with water and food during a 12 h dark/light cycle. After acclimatizing for a week, the animals were randomly assigned to two groups: model (TAC) and control (sham). In the TACgroup, the aortic arch of the mice was constricted using a 27 G needle and 6.0 sutures. The Sham group was operated in the same way as the TAC group without ligation of the aorta. All mice were housed for four weeks for further research."
item3550 "To establishment the xenograftmodels, twenty mice were randomly divided into four groups, each of which contained five nude mice. In the sh-NC group, 4 × 106 A549 cells infected with LV-sh-NC were diluted in 100 μl of PBS and injected subcutaneously into nude mice. In the sh-DNMT1 group, 4 × 106 A549 cells infected with LV-sh-DNMT1 were diluted in 100 μl of PBS and injected subcutaneously into nude mice. In the sh-DNMT1 + sh-FOXO3a group, 4 × 106 A549 cells infected with LV-sh-DNMT1 and LV-sh-NC were diluted in 100 μl of PBS and injected subcutaneously into nude mice."
item3551 .
item3552 .
item3553 "Eight-week-old male BALB/c mice were intraperitoneally injected with streptozotocin (50 mg/kg) for five days to induce diabetes. Two weeks post-injection, blood glucose levels were measured using a blood glucose monitor. Successful induction of diabetes was confirmed when the blood glucose level exceeded 16.7 mmol/L and was sustained for an additional four weeks before creating full-thickness cutaneous wounds."
item3554 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25°C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
item3555 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25°C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
item3556 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25°C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
item3557 "These mice were provided with unrestricted access to both water and food, following a 12-h light and 12-h dark cycle. Subcutaneous injections of stably transfected K1 cells (2 × 106 cells per mouse) were administered in the armpits of the mice. Once the tumors became palpable, their volume was measured every three days and calculated using the formula: length × width2 × 0.5. The tumor weight was detected after the mice were sacrificed."
item3558 "These mice were provided with unrestricted access to both water and food, following a 12-h light and 12-h dark cycle. Subcutaneous injections of stably transfected K1 cells (2 × 106 cells per mouse) were administered in the armpits of the mice. Once the tumors became palpable, their volume was measured every three days and calculated using the formula: length × width2 × 0.5. The tumor weight was detected after the mice were sacrificed."
item3559 .
item3560 "All rats were housed under a 12 h light/dark cycle with constant temperature about 25 °C and relative humidity approximating 55%. The rats had free access to food and water for 10 days prior to the experiment. After that, the renal ischemia-reperfusion injury rat model was established. SD rats were anesthetized with an intraperitoneal injection of pentobarbital sodium (25 mg/kg). Then an incision was made in the rat abdomen, and the right kidney was removed for nephrectomy. The left kidney was exposed after a midline incision, the renal artery was clamped for 45 min with a nontraumatic clamp, and renal blood flow was then restored."
item3561 .
item3562 .
item3563 .
item3564 .
item3565 "Male BALB/c mice (SJA Laboratory Animal Company, Hunan, China) with age of 6-8 weeks were used in this study to establish COPD model. Mice were housed in individually ventilated cages under a pathogen-free condition, with ad libitum access to food and water. Animal welfare was monitored daily, and all efforts were made to minimize suffering. All animal procedures were conducted in accordance with the guidelines for use of laboratory animals, with approval from the Institutional Animal Care and Use Committee at Jiangxi Provincial People's Hospital (The First Affiliated Hospital of Nanchang Medical College)."
item3566 "Male BALB/c mice (SJA Laboratory Animal Company, Hunan, China) with age of 6-8 weeks were used in this study to establish COPD model. Mice were housed in individually ventilated cages under a pathogen-free condition, with ad libitum access to food and water. Animal welfare was monitored daily, and all efforts were made to minimize suffering. All animal procedures were conducted in accordance with the guidelines for use of laboratory animals, with approval from the Institutional Animal Care and Use Committee at Jiangxi Provincial People's Hospital (The First Affiliated Hospital of Nanchang Medical College)."
item3567 "The left internal carotid artery of the rats was isolated. Then the ligation of middle cerebral artery was performed by a 4/0 surgical nylon monofilament to occlude the blood flow. 2 h later, we removed the filament to restore the blood reperfusion for 24 h. During the entire operation, they were kept at 37.0 ± 0.5 °C. The laser Doppler flowmetry (Transonic Systems, Ithaca, NY, USA) was applied to confirm the regional ischemia and reperfusion. Rats were decapitated after 24 h of reperfusion."
item3568 .
item3569 "A total of 3x106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μL of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2×longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
item3570 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3571 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3572 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3573 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3574 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3575 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3576 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3577 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3578 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3579 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3580 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3581 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3582 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3583 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3584 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3585 "Rats were housed at 23°C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
item3587 .
item3588 .
item3589 .
item3590 .
item3591 .
item3592 .
item3593 .
item3594 .
item3595 .
item3596 .
item3597 .
item3598 .
item3599 .
item3600 .
item3601 .
item3602 .
item3603 .
item3604 .
item3605 .
item3606 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+ mice, and Mettl14+/+ mice were intercrossed to obtain Mettl14-conventional knockout mice. Sex of embryos was determined."
item3607 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+ mice, and Mettl14+/+ mice were intercrossed to obtain Mettl15-conventional knockout mice. Sex of embryos was determined."
item3608 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+ mice, and Mettl14+/+ mice were intercrossed to obtain Mettl16-conventional knockout mice. Sex of embryos was determined."
item3609 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+ mice, and Mettl14+/+ mice were intercrossed to obtain Mettl17-conventional knockout mice. Sex of embryos was determined."
item3610 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+mice, and Mettl14+/+ mice were intercrossed to obtain Mettl18-conventional knockout mice. Sex of embryos was determined."
item3611 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/+ mice, and Mettl14+/+ mice were intercrossed to obtain Mettl19-conventional knockout mice. Sex of embryos was determined."
item3612 "Mice were housed in standard conditions in the Laboratory Animal Center of Zhejiang University on a 12 h light/dark schedule with free access to food and water. The pregnant mice were purchased from Shanghai SLAC Laboratory Animal Company, China. All animal experiments were conducted according to protocols approved by the Zhejiang University Animal Care and Use Committee."
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item3630 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
item3631 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μL gas-tight syringe (Hamilton) was then used to inject 10 μL of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
item3632 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μL gas-tight syringe (Hamilton) was then used to inject 10 μL of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
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item3648 "For subcutaneous inoculation, 1 × 106 control or METTL16-depleted CCA cells suspended in 100 μL PBS mixed with matrix gel (BD, 356,234) at 1:1 ratio was injected into the mice's flanks. Tumor sizes were measured at the relevant time intervals. At the end of the study, the mice were euthanized, and the tumors were dissected and weighed. For intrahepatic inoculation, 1 × 106 control or METTL16-depleted CCLP1 cells suspended in 10 μL PBS mixed with matrix gel (BD, 356,234) at a 1:1 ratio was implanted into the livers of NOD-SCID mice. The mice were sacrificed 8 weeks after injection, their livers were surgically dissected, and the liver/body weight ratios were evaluated."
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item3793 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
item3794 .
item3795 "Mice were randomly divided into control, sh-NC, and sh-RMRP groups by a researcher who was blinded to our experimental design. Each group contains 6 mice. U251/TMZ cells (1 × 107 cells/mouse), U251/TMZ cells infected with sh-NC (1 × 107 cells/mouse), or U251/TMZ cells infected with sh-RMRP (1 × 107 cells/mouse) were injected subcutaneously into the right-back of mice in the control, sh-NC, or sh-RMRP group, respectively. TMZ (25 mg/kg body weight) was intraperitoneally injected into mice every other day for 14 days when tumor volumes reached approximately 100 mm3."
item3796 "Control or NBAT1 overexpressing Hep3B cells (107 cells/mouse, n = 5 per group) were injected subcutaneously into the the flanks of BALB/c nude mice. Their tumor growth was monitored every 5 days. The tumor volumes were calculated by the formula 0.5 ×width2 × length. After 35 days, the mice were sacrificed, and the tumors were isolated and weighed."
item3797 .
item3798 .
item3799 .
item3800 .
item3801 .
item3802 Harvested cells were resuspended in PBS and each side of mouse was injected about 1 × 106 cells. Tumor volume was estimated every four days and calculated as 0.5 × length × width2.
item3803 Harvested cells were resuspended in PBS and each side of mouse was injected about 1 × 106 cells. Tumor volume was estimated every four days and calculated as 0.5 × length × width2.
item3804 .
item3805 .
item3806 .
item3807 .
item3808 .
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item3810 .
item3811 .
item3812 Each group contained 15 mice. Mice were injected subcutaneously with 3×105 cells in the right axillary fossa. Tumor size was measured every five days until 60 days after injection.
item3813 "NOD-SCID mice in each experimental group were injected with 2 × 106 HCT-116-Luc-LINC00266-1-ORF-Flag, HCT-116-Luc-LINC00266-1-5'U, or HCT-116-Luc-LINC00266-1-MT cells through the tail vein (n = 5). The metastatic foci were visualized 9 weeks after implantation using an IVIS 200 Imaging System (Xenogen)."
item3814 .
item3815 .
item3816 .
item3817 .
item3818 .
item3819 .
item3820 "Female BALB/c nude mice aged 4 weeks old were adopted. In the subcutaneous xenograft tumor, GC cells (2 × 106/200 μl) were subcutaneously injected into the mice flank."
item3821 .
item3822 .
item3823 .
item3824 .
item3825 .
item3826 .
item3827 .
item3828 Subcutaneous xenograft model in nude mice: Each group of HeLa cells was prepared as a cell suspension at a concentration of 2 × 107/mL.
item3829 "Ten mice were randomly divided into two groups, and C4-2-pZXE-circDDIT4 or C4-2-pZXE-NC cell suspension was concentrated to 5 × 106 cells/100 μL PBS and then injected into the flanks of the nude mice. After 20 days, the mice were euthanized, and the xenograft tumors were harvested, weighed, and photographed."
item3830 .
item3831 .
item3832 .
item3833 .
item3834 .
item3835 .
item3836 "For the subcutaneous tumor growth assay, 6-8 weeks old nude mice were sorted into six groups (n = 5 per group) at random. Each group received one of the following treatments bilaterally into the subcutaneous tissue of the flank: MHCC97H cells alone (1×10^6), exosomes from M0 macrophages incubated with MHCC97H cells (1×10^6), MHCC97H with AS1-KD exosome-treated M0 macrophages (1×10^6), and MHCC97H coupled with exosomes from siNC treated M2 macrophages (1×10^6) or siIL-6 treated M2 macrophages (1×10^6)."
item3837 .
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item3841 .
item3842 .
item3843 .
item3844 .
item3845 .
item3846 .
item3847 .
item3848 .
item3849 "Ten BALB/C nude mice (4 weeks old, female) were injected in 1 × 106 HCT116 cells in 100 uL PBS at each side. The tumor size was detected every four days after the injection of cells and calculated according to the formula."
item3850 .
item3851 .
item3852 .
item3853 .
item3854 "Mice were injected with Molm13-luc-GFP AML cells treated with sh-NC, sh-MEG3, sh-NC + AraC, sh-MEG3 + AraC, oe-NC, oe-MEG3, oe-NC + AraC, or oe-MEG3 + AraC, respectively (n = 8 in each group).NSG mice were intravenously injected with cells treated by Molm13-luc-GFP or Molm13 R-luc-GFP (2 × 106 cells/100 μL)."
item3855 "Mice were injected with Molm13-luc-GFP AML cells treated with sh-NC, sh-MEG3, sh-NC + AraC, sh-MEG3 + AraC, oe-NC, oe-MEG3, oe-NC + AraC, or oe-MEG3 + AraC, respectively (n = 8 in each group).NSG mice were intravenously injected with cells treated by Molm13-luc-GFP or Molm13 R-luc-GFP (2 × 106 cells/100 μL)."
item3856 .
item3857 "In order to develop a xenograft model, 12 nude mice (1 × 107cells per 200 μL of FBS-free medium) were injected subcutaneously with CAOV3 cells."
item3858 7 × 106 SGC7901 cells stably overexpressed LNC942 or pCDH empty vector (LNC942/NC) were suspended in 125-μl PBS and inoculated into the right dorsal flank of 5-week-old female nude mice (n = 5 per group).
item3859 "A total of 5 × 105 U87MG cells stably expressing firefly luciferase (Fluc) with indicated treatments were injected into the mouse brain at 2 mm lateral, 2 mm posterior to the bregma, and 2 mm depth via a stereotaxic apparatus."
item3860 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 5 per group). A549 cells (2 × 106) that had been stably transfected with sh-LCAT1 or scramble were implanted subcutaneously into the nude mice."
item3861 Stable cells were selected with 2 μg/ml puromycin for two weeks. Male athymic BALB/c nude mice (4-5 weeks old) were used. Subcutaneous tumor growth assays were used for growth assay. A total of 5 × 106 cells was subcutaneously injected into the nude mice (n = 3). The tumor volume was measured. The mice were sacrificed 4 weeks after injection. The tumor tissues were isolated and weighed. A tail vein injection model was used for metastasis assays. A total of 5 × 105 cells were injected into tail vein of nude mice (n = 3).
item3862 "Or tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
item3863 "Or tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
item3864 "Or tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
item3865 "Or tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
item3866 "Approximately 2 × 106 NSCLC cells (A549), transfected with sh-DLGAP1-AS2 or controls, were suspended in 100 μl PBS and then injected into flank of male BALB/c nude mice (5-wk old)."
item3867 "Six-week-old male nude BALB/C mice were randomly divided into two groups, NC and sh-HCP5#1 groups. 2 × 106 EC109 cells were subcutaneously injected into nude mice and grown for 5 weeks."
item3868 "ShRNA control (empty vector) and sh-H19 were transfected into BGC-823 cells for 24 h. The cells were collected after digested by trypsin. The cells were washed using phosphate buffered saline (PBS) (Gibco, USA) twice and resuspended with PBS before being subcutaneously injected into nude mice."
item3869 .
item3870 "The mice were subcutaneously inoculated with 6 × 107 HepG2 cells stably transfected with pLV-circGPR137B and empty vectors or Sk-hep-1 cells stably transfected with pLV-sh-circGPR137B/sh-NC. HepG2 cells stably transfected with circGPR137B or pcDNA3.1 were cultured in complete medium. When the cells were 70% confluent, the medium was replaced with fresh medium to remove dead and detached cells. Subsequently, 6 × 107 cells were used to inject into mice via the tail vein. Each mouse was injected with 100ul (6× 106 cells)."
item3871 "The mice were subcutaneously inoculated with 6 × 107 HepG2 cells stably transfected with pLV-circGPR137B and empty vectors or Sk-hep-1 cells stably transfected with pLV-sh-circGPR137B/sh-NC. HepG2 cells stably transfected with circGPR137B or pcDNA3.1 were cultured in complete medium. When the cells were 70% confluent, the medium was replaced with fresh medium to remove dead and detached cells. Subsequently, 6 × 107 cells were used to inject into mice via the tail vein. Each mouse was injected with 100ul (6× 106 cells)."
item3872 "Male BALb/c nude mice (three mice per group) were bought from Guangdong Medical Laboratory Animal Center, and indicated HCC cells were subcutaneously injected into the flanks of nude mice at 2 × 106 cells per site."
item3873 "4-6 weeks old male BALB/c nude mice were used in present study. Lymph node metastasis animal model was established by injecting 2 × 105 RPRD1B-overexpressed HGC27 and shRPRD1B-transfected AGS cells into right hind footpad of nude mice, respectively."
item3874 .
item3875 "Male C57BL/6J mice (6-8 weeks old and 18-21 g weight) were randomly divided into 3 groups, including ctrl + ad-NC, LPS + ad-NC and LPS + ad-sh-Mir22hg."
item3876 "He AMI model was induced by permanent ligation of the left coronary artery anterior descending with a 7-0 surgical suture as previously described , and then miR-636 antagomir (10 mg/kg; RiboBio) was injected through the tail vein of mice or the nanoparticles with DKK2 plasmids were administered intraperitoneally in a volume of 200 μL into AMI mice."
item3877 "A total of 5×106 SK-Hep-1 cells (shNC, sh1089-1, or sh1089-2) into fossa axillaries of 5-week-old male nude BALB/c mice (Vital River Laboratory; n = 5 per group).in vivo, we injected a total of 2×107 SK-Hep-1 cells with stable LINC01089-KD (shNC, sh1089-1, or sh1089-2) or LINC01089-OE (NC or lnc1089) into the middle of the lower abdomen of male nude BALB/c mice (Vital River Laboratory; n = 6 per group).To investigate the involvement of LINC01089 in hematogenous metastases, a total of 1×107 SK-Hep-1 cells with stable firefly luciferase expression (shNC, sh1089-1, sh1089-2, NC, or lnc1089) were injected into tail vein of male nude BALB/c mice (Vital River Laboratory; n = 4 per group)."
item3878 .
item3879 "Experiment 1:Eighteen rats were allocated to Control, DN, and DN + NAC (an inhibitor of ROS) groups (n = 6/group) at random. Rats in the DN and DN + NAC groups were subjected to high-fat diets (45% kcal fat), whereas control rats were normally bred with 10% kcal fat. Experiment 2:Twelve rats were randomly divided into DN + PBS and DN + Exo groups (n = 6/group). Experiment 3:Twenty-four rats were randomly assigned to DN, DN + Exo, DN + Exo-mimic NC, and DN + Exo-miR-204 mimic groups (n = 6/group)."
item3880 "For subcutaneous tumor models, 1.5 × 106 HUH7 cells (e.g., negative control, AC115619-overexpressing cells) in 50 μL PBS with 50 μL Matrigel were administered to male BALB/c nude mice (4-6 weeks of age) by subcutaneous injection and cell line-derived xenografts (CDX) were established.For lung metastasis models, luciferase-tagged lentivirus transfected into HUH7 cells (1 × 106/100 μL PBS) was injected into male BALB/c nude mice (4-6 weeks of age) via tail vein."
item3881 "For subcutaneous tumor models, 1.5 × 106 HUH7 cells (e.g., negative control, AC115619-overexpressing cells) in 50 μL PBS with 50 μL Matrigel were administered to male BALB/c nude mice (4-6 weeks of age) by subcutaneous injection and cell line-derived xenografts (CDX) were established.For lung metastasis models, luciferase-tagged lentivirus transfected into HUH7 cells (1 × 106/100 μL PBS) was injected into male BALB/c nude mice (4-6 weeks of age) via tail vein."
item3882 .
item3883 BEL-7404 cells or BEL-7404 miR4458HG-KO cells were infused in the right flank of randomly selected 4-week-old male BALB/c mice.
item3884 BEL-7404 cells or BEL-7404 miR4458HG-KO cells were infused in the right flank of randomly selected 4-week-old male BALB/c mice.
item3885 "To investigate chronic Cd-induced mitochondrial dysfunction and neurotoxicity in vivo, C57BL/6J mice were randomly assigned to 2 groups: (i) control group mice (n = 20) and (ii) Cd exposure group mice (n = 20)."
item3886 .
item3887 .
item3888 "Each nude mouse from the TE-1 group was injected with 0.2 mL TE-1 cell suspensions (1 × 106 cells), and each mouse from the sh-LINC00858 group or the sh-NC group was injected respectively with an equal volume of TE-1 cells stably transfected with sh-LINC00858 or sh-NC."
item3889 .
item3890 "One microliter of AAV [pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine, AAV9-hSyn-EGFP-miR-NC-knockdown (KD) or AAV9-hSyn-EGFP-miR-200c-3p-KD; 5 × 1012 v.g/mL] was injected into the mouse motor cortex (M1; 1 mm anterior, 1.5 mm lateral to the bregma and at a depth of 1 mm)."
item3891 .
item3892 .
item3893 .
item3894 A concentration of 1 × 107 CAL62 cells stably transfected with OE METTL3 or OE METTL3/shPAX8-transfected CAL-62 cells were injected subcutaneously into the dorsal flanks of the mice (five mice per group) to establish a xenograft model.
item3895 A concentration of 1 × 107 CAL62 cells stably transfected with OE METTL3 or OE METTL3/shPAX8-transfected CAL-62 cells were injected subcutaneously into the dorsal flanks of the mice (five mice per group) to establish a xenograft model.
item3896 .
item3897 .
item3898 .
item3899 "BALB/c nude mice were purchased from the Slac Laboratories (Shanghai, China) and randomly divided into two groups. The xenograft mouse model was generated via tail-vein injection of METTL14-expressing T24 cells (5 × 105 cells per mouse) into experimental mice while those injected with pcDNA3.1 empty vector transfected cells as control."
item3900 "BALB/c nude mice were purchased from the Slac Laboratories (Shanghai, China) and randomly divided into two groups. The xenograft mouse model was generated via tail-vein injection of METTL14-expressing T24 cells (5 × 105 cells per mouse) into experimental mice while those injected with pcDNA3.1 empty vector transfected cells as control."
item3901 .
item3902 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
item3903 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
item3904 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
item3905 "Pregnant C57/BL6 were randomly assigned into four groups (each n = 10): (1) corn oil group, (2) BaP group, (3) BaP +AS-NC group, (4) BaP +AS-hz06 group."
item3906 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
item3907 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
item3908 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
item3909 .
item3910 .
item3911 .
item3912 .
item3913 .
item3914 .
item3915 .
item3916 "40 C57BL/6 J male mice (8 weeks old, 20-22 g) obtained from SLAC Experimental Animal Co., Ltd. were randomly assigned into five groups (n = 8): sham, I/R, I/R + Exos, I/R + inhibitor NC-Exos, and I/R + miR-381 inhibitor-Exos."
item3917 "For the xenograft model, 100μL cell suspensions containing 1× 106 CRC cells sin 100 μLPBS were injected into the armpits of the mouse limbs which divided into six groups (shNC, shHMGCS2, shLOC+OE-HMGCS2, shLOC, shHMGCS2+OE-IGF2BP1, shLOC+OE-GF2BP1)."
item3918 "To examine the effects of piRNA-14633 on subcutaneous xenograft growth, BALB/c nude mice (Beijing Vital River Laboratory Animal Technology) were subcutaneously injected with 0.1 mL of cell suspension containing 2 × 106 cells. Tumor volume (mm3) was measured every 4 days using a Vernier caliper and calculated as 0.4 x (short length)2 × long length."
item3919 .
item3920 .
item3921 .
item3922 "MDA-MB-231 cells (1×106 cells/mouse) were mixed with an equivalent number of NFs/Ctrl, CAFs/shNC, CAFs/sh lncSNHG5, CAFs/shZNF281, CAFs/sh lncSNHG5/ZNF281, CAFs/RS102895, CAFs/Maraviroc or CAFs/Cenicriviroc in 200 μl PBS:Matrigel at a ratio of 1:1."
item3923 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μL of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
item3924 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μL of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
item3925 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μL of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
item3926 "Logarithmic phase PDAC cells (4 × 106/100 μl) were infected using lentiviruses possessing constructs. Then, the nude mice (n = 5 per group) were inoculated into the dorsal flank subcutaneously."
item3927 "The KYSE-150 cells were subcutaneously inoculated on the right side at a dosage of 106 cells per mouse. After the tumor grew to approximately 50 mm3, mice were randomly divided into four groups (n = 6): Control, CisR-exo, Cis, and Cis + CisR-exo. Then, normal saline or cisplatin (20 mg/kg; twice a week) was intratumorally injected alone or combined with CisR-exos (10 μg; once every two days)."
item3928 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
item3929 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
item3930 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μl of PBS plus 50 μl of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
item3931 .
item3932 .
item3933 "BGC-823 (3 × 106) cells that stably expressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected into the left side of each mouse. For cell metastasis experiments in vivo, BGC-823 (3 × 106) cells that stably overexpressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected through tail vein."
item3934 "BGC-823 (3 × 106) cells that stably expressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected into the left side of each mouse. For cell metastasis experiments in vivo, BGC-823 (3 × 106) cells that stably overexpressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected through tail vein."
item3935 "Mice were blindly grouped into sh-NC, sh-LRRC75A-AS1, OE-NC, and OE-LRRC75A-AS1 groups (n = 7 per group). sh-NC or sh-LRRC75A-AS1-transfected HeLa cells and OE-NC or OE-LRRC75A-AS1-transfected CaSki cells were subcutaneously injected into the right flank (1 × 106 cells in 200 μL PBS/mouse)."
item3936 "Six to eight weeks old male BALB/c nu/nu mice were used. For orthotopic implantation, 1 × 107 PBS suspended cells was injected subcutaneously. For the spleen injection model, 5 × 105 cells were injected into the spleen through an incision on the left side of abdomen."
item3937 "When the xenografted tumors grew up to 50 mm3, 18 mice were randomly divided into three groups (n = 6 mice /group): NC group (mice have systematically injected NC lentivirus through the lateral tail vein), sh-OIP5-AS1-1 group (mice have systematically injected sh-OIP5-AS1-1 lentivirus through the lateral tail vein), sh-OIP5-AS1-2 group (mice have systematically injected sh-OIP5-AS1-2 lentivirus through the lateral tail vein).A total of 18 6-week-old healthy male NOD/SCID mice (weighing 20 ± 2 g) were randomly assigned to one of the three groups (n = 6 mice /group) and injected through the tail vein with 1 × 106 NC, sh-OIP5-AS1-1, or sh-OIP5-AS1-2 stable AGS cells or Ctrl or OIP5-AS1 stable MGC823 cells co-transfected with lentiviruses carrying the luciferase reporter gene."
item3938 .
item3939 .
item3940 .
item3941 The cell mass (5 × 106) was dissolved in 200 μl PBS and subcutaneously injected into the left side of each mouse.
item3942 The cell mass (5 × 106) was dissolved in 200 μl PBS and subcutaneously injected into the left side of each mouse.
item3943 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group)."
item3944 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group)."
item3945 .
item3946 .
item3947 .
item3948 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
item3949 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
item3950 A total of 1 × 106 luciferase-labeled tumor cells were injected subcutaneously with SN-exo or Ctr-exo.
item3951 .
item3952 "For subcutaneous HCC mice xenograft model, the cell suspension was injected into the right flank of the mice (n = 5 per group)."
item3953 "For subcutaneous HCC mice xenograft model, the cell suspension was injected into the right flank of the mice (n = 5 per group)."
item3954 Xenograft mouse models were established by subcutaneously inoculating 1 × 107 transfected ACHN cells into mouse left flank.
item3955 "To establish a murine subcutaneous tumor model, sh-PLAGL2 or sh-PLAGL2 + oe-Snail HGC-27 stable transfected HGC-27 cells (5 × 106 cells/kg) subcutaneously to the right of the dorsal midline of mice (n = 6 for each)."
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item3979 "In the control mice or ADR mice group, the parental or chemo-resistant K562 cells were infected with LV-shCtrl. In the ADR + shLINC00470 group, the chemo-resistant K562 cells were infected with LV- shLINC00470. These cells were injected, respectively, into these 5-week-old mice subcutaneously."
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M6ACROT00012 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old). Then, the tumour volumes were measured every 1 week. After 4 weeks, mice were sacrificed and the tumours were weighed. To detect the effect of NKILA on CAA metastasis, 1 × 105 control and NKILA-depleted HuCCT1 cells (n = 5 per group) were injected into the tail vein of nude mice. After 8 weeks, mice were sacrificed and the lung tissues were used for H&E staining. The metastatic nodules in lung tissues were counted under a microscope (Zeiss). The mice were maintained under specified pathogen-free (SPF) conditions."
M6ACROT00013 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old). Then, the tumour volumes were measured every 1 week. After 4 weeks, mice were sacrificed and the tumours were weighed. To detect the effect of NKILA on CAA metastasis, 1 × 105 control and NKILA-depleted HuCCT1 cells (n = 5 per group) were injected into the tail vein of nude mice. After 8 weeks, mice were sacrificed and the lung tissues were used for H&E staining. The metastatic nodules in lung tissues were counted under a microscope (Zeiss). The mice were maintained under specified pathogen-free (SPF) conditions."
M6ACROT00014 "To develop the syngeneic model, 1 × 106 MC38-Ctrl or MC38-Gpx4KD cells were injected subcutaneously into the right flank of C57BL/6 mice, respectively. These two groups of mice were randomly and blindly allocated into separate groups 12 days after cell injection. Subsequently, either an anti-PD-1 antibody (5 mg/kg per mouse) or an IgG isotype was administered intraperitoneally every 2 days until the conclusion of the observation period. "
M6ACROT00015 "To develop the syngeneic model, 1 × 106 MC38-Ctrl or MC38-Gpx4KD cells were injected subcutaneously into the right flank of C57BL/6 mice, respectively. These two groups of mice were randomly and blindly allocated into separate groups 12 days after cell injection. Subsequently, either an anti-PD-1 antibody (5 mg/kg per mouse) or an IgG isotype was administered intraperitoneally every 2 days until the conclusion of the observation period. "
M6ACROT00016 "During the first immunization, 100 μ l of modeling reagents were injected into the tail root and subcutaneous area of the back of the rats. Then, incomplete Freund's adjuvant was used as an emulsifier for bovine type II collagen, fully mixed, and subjected to secondary immunization on the 21st day."
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M6ACROT00050 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00051 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00052 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00053 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00054 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00055 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00056 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00057 "Six- to eight-week-old C57BL/6 mice were purchased from Cyagen Biosciences, Inc. (Cyagen, Suzhou, China). Alkbh3/ C57BL/6 mice were generated via the CRISPR/Cas9 system. Single-guide RNAs (sgRNAs) were designed to target exons 3 to 5 of Alkbh3 and were coinjected with Cas9 into the zygotes. The pups obtained were genotyped by PCR. After genotyping, the F0 mice were subjected to serial mating to generate homozygous mutant offspring."
M6ACROT00058 "CAOV3 cells overexpressing SNORD9 or control CAOV3 cells (1 × 107) were suspended in FBS-free media and injected subcutaneously into BALB/c nude mice. Mice were euthanized every four days post-injection. Besides, OVCAR3 cells (5 × 106) were injected subcutaneously (n = 12), when most of the size of the tumor reached 5 mm in diameter, nude mice were randomly divided into two groups, followed by ASO-SNORD9 or ASO-NC (250 nM/kg/week) administered subcutaneously for 4 weeks. "
M6ACROT00059 Each mouse was injected with 5 × 106 MIR4435-2HG overexpressed Huh7 cells on the left armpit and control on the right armpit (six mice per group).
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M6ACROT00063 "2 × 106 U87MG cells already expressing shscr or shADAR1 were subcutaneously injected in the flank of 6-week-old nude mice. For orthotopic mouse models, 2 × 105 shscr and shADAR1 U87MG glioblastoma cells were intracranially injected into male NOD/SCID mice."
M6ACROT00064 "2 × 106 U87MG cells already expressing shscr or shADAR1 were subcutaneously injected in the flank of 6-week-old nude mice. For orthotopic mouse models, 2 × 105 shscr and shADAR1 U87MG glioblastoma cells were intracranially injected into male NOD/SCID mice."
M6ACROT00065 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group). Tumor growth was measured by measuring the width (W) and length (L) with calipers, and the volume (V) of the tumor was figured using the criterion V = (W2 × L)/2. At 42 days after injection, the mice were euthanized and tumors were removed and weighed. The tumor samples were further analyzed with IHC and Western blot assay. The animal studies were performed according to the institutional ethics guidelines for animal experiments and approved by the Chongqing Medical University Animal Care and Use Committee."
M6ACROT00066 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group). Tumor growth was measured by measuring the width (W) and length (L) with calipers, and the volume (V) of the tumor was figured using the criterion V = (W2 × L)/2. At 43 days after injection, the mice were euthanized and tumors were removed and weighed. The tumor samples were further analyzed with IHC and Western blot assay. The animal studies were performed according to the institutional ethics guidelines for animal experiments and approved by the Chongqing Medical University Animal Care and Use Committee."
M6ACROT00067 "The rats were anaesthetized via strictly aseptic techniques during surgery. The knee joint was opened laterally by dislocating the patellar tendon, and a full thickness osteochondral defect (3-mm in diameter) was created in the trochlear groove of the femur. Then, approximately 107BMSCs were mixed with 1 ml hydrogel matrix (Coring, Cat#354250) and were injected into the defect."
M6ACROT00068 "The rats were anaesthetized via strictly aseptic techniques during surgery. The knee joint was opened laterally by dislocating the patellar tendon, and a full thickness osteochondral defect (3-mm in diameter) was created in the trochlear groove of the femur. Then, approximately 107BMSCs were mixed with 1 ml hydrogel matrix (Coring, Cat#354250) and were injected into the defect."
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M6ACROT00073 The mice for the xenograft tumour-growth assays and tail-vein assays of cancer metastasis were obtained from Charles River Laboratories. The mice for the PDX model were obtained from and established at the NBRI.
M6ACROT00074 The mice for the xenograft tumour-growth assays and tail-vein assays of cancer metastasis were obtained from Charles River Laboratories. The mice for the PDX model were obtained from and established at the NBRI.
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M6ACROT02001 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
M6ACROT02002 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
M6ACROT02003 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
M6ACROT02004 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
M6ACROT02005 "Following 1 week of adaptive feeding, the mice were randomly divided into CCl4 + siCON and CCl4 + siYTHDF1 groups. Both groups were injected intraperitoneally with 5 ml/kg of 10% carbon tetrachloride (CCl4) twice a week for 4 weeks, to establish liver fibrosis."
M6ACROT02006 .
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M6ACROT02013 " An aliquot of the vector at 1011 genome equivalents was prepared in 20-30 μL of HBSS and isoflurane anesthesia followed by nasal drops. Mice were randomly divided into five groups as follows: normoxic environment plus control vector group (NOR + NC, n = 20), hypoxic environment plus control vector group (HYP + NC, n = 10), hypoxic environment plus FENDRR TFO2 adenovirus group (HYP + FENDRR TFO2, n = 10), normoxic environment plus FENDRR TFO2 adenovirus group (NOR + FENDRR TFO2, n = 10). "
M6ACROT02014 .
M6ACROT02015 .
M6ACROT02016 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
M6ACROT02017 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
M6ACROT02018 "All the animal studies and protocols followed the institutional guidelines of the First Affiliated Hospital, School of Medicine, Zhejiang University."
M6ACROT02019 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02020 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02021 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02022 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02023 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02024 "Male C57BL/6 mice (aged 6-8 weeks) were acclimated with the Lieber-DeCarli liquid diet control ad libitum for 1 week. Afterward, mice received either Lieber-DeCarli diet ethanol (6% vol/vol) or Lieber-DeCarli diet control (isocaloric maltose dextrin) for 4 and 8 weeks."
M6ACROT02025 .
M6ACROT02026 .
M6ACROT02027 "Sprague Dawley rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium at 0.2 mL/100 g. The thoracic aorta was separated under aseptic conditions, and was placed in pre-cooled DMEM medium, and the tunica media was separated under a microscope. The tunica media were cut into small pieces of 0.5-1 mm2, and digested with type II collagenase (2 mg/mL) in a 37 ° C water bath. The cells were collected by centrifugation, and resuspended in DMEM medium containing 20% FBS."
M6ACROT02028 "Sprague Dawley rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium at 0.2 mL/100 g. The thoracic aorta was separated under aseptic conditions, and was placed in pre-cooled DMEM medium, and the tunica media was separated under a microscope. The tunica media were cut into small pieces of 0.5-1 mm2, and digested with type II collagenase (2 mg/mL) in a 37 ° C water bath. The cells were collected by centrifugation, and resuspended in DMEM medium containing 20% FBS."
M6ACROT02029 "Sprague Dawley rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium at 0.2 mL/100 g. The thoracic aorta was separated under aseptic conditions, and was placed in pre-cooled DMEM medium, and the tunica media was separated under a microscope. The tunica media were cut into small pieces of 0.5-1 mm2, and digested with type II collagenase (2 mg/mL) in a 37 ° C water bath. The cells were collected by centrifugation, and resuspended in DMEM medium containing 20% FBS."
M6ACROT02030 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02031 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02032 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02033 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02034 .
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M6ACROT02040 "8-week-old male C57BL/6 mice were randomly divided into four groups: Ctrl+AAV-NC group (n = 6), Ctrl+AAV-OT group (n = 6), MIA+AAV-NC group (n = 8), and MIA+ AAV-OT group (n = 8). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. Control mice were given normal saline in the same volume."
M6ACROT02041 "8-week-old male C57BL/6 mice were randomly divided into four groups: Ctrl+AAV-NC group (n = 6), Ctrl+AAV-OT group (n = 6), MIA+AAV-NC group (n = 8), and MIA+ AAV-OT group (n = 8). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. Control mice were given normal saline in the same volume."
M6ACROT02042 "Slack Laboratory Animal Co., Ltd. (Shanghai, China) supplied the six-week-old C57BL/6 male mice. Animals were reared at constant temperature (21 ± 1 °C) and maintained in a room with water and food during a 12 h dark/light cycle. After acclimatizing for a week, the animals were randomly assigned to two groups: model (TAC) and control (sham). In the TACgroup, the aortic arch of the mice was constricted using a 27 G needle and 6.0 sutures. The Sham group was operated in the same way as the TAC group without ligation of the aorta. All mice were housed for four weeks for further research."
M6ACROT02043 "To establishment the xenograftmodels, twenty mice were randomly divided into four groups, each of which contained five nude mice. In the sh-NC group, 4 × 106 A549 cells infected with LV-sh-NC were diluted in 100 μ l of PBS and injected subcutaneously into nude mice. In the sh-DNMT1 group, 4 × 106 A549 cells infected with LV-sh-DNMT1 were diluted in 100 μ l of PBS and injected subcutaneously into nude mice. In the sh-DNMT1 + sh-FOXO3a group, 4 × 106 A549 cells infected with LV-sh-DNMT1 and LV-sh-NC were diluted in 100 μ l of PBS and injected subcutaneously into nude mice."
M6ACROT02044 .
M6ACROT02045 .
M6ACROT02046 "Eight-week-old male BALB/c mice were intraperitoneally injected with streptozotocin (50 mg/kg) for five days to induce diabetes. Two weeks post-injection, blood glucose levels were measured using a blood glucose monitor. Successful induction of diabetes was confirmed when the blood glucose level exceeded 16.7 mmol/L and was sustained for an additional four weeks before creating full-thickness cutaneous wounds."
M6ACROT02047 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25 ° C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
M6ACROT02048 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25 ° C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
M6ACROT02049 "Healthy adult ICR male (aged: 8 weeks old; weighing: 40- 70 g) and female mice (aged: 8 weeks old; weighing: 40-60 g) were obtained from Hunan SJA Laboratory Animal Co., Ltd. (Hunan, China). These mice were housed in the SPF laboratory at 25 ° C and 55% humidity under a 12-h light/dark cycle, with ad libitum access to food and water for acclimatization."
M6ACROT02050 "These mice were provided with unrestricted access to both water and food, following a 12-h light and 12-h dark cycle. Subcutaneous injections of stably transfected K1 cells (2 × 106 cells per mouse) were administered in the armpits of the mice. Once the tumors became palpable, their volume was measured every three days and calculated using the formula: length × width2 × 0.5. The tumor weight was detected after the mice were sacrificed."
M6ACROT02051 "These mice were provided with unrestricted access to both water and food, following a 12-h light and 12-h dark cycle. Subcutaneous injections of stably transfected K1 cells (2 × 106 cells per mouse) were administered in the armpits of the mice. Once the tumors became palpable, their volume was measured every three days and calculated using the formula: length × width2 × 0.5. The tumor weight was detected after the mice were sacrificed."
M6ACROT02052 .
M6ACROT02053 "All rats were housed under a 12 h light/dark cycle with constant temperature about 25 ° C and relative humidity approximating 55%. The rats had free access to food and water for 10 days prior to the experiment. After that, the renal ischemia-reperfusion injury rat model was established. SD rats were anesthetized with an intraperitoneal injection of pentobarbital sodium (25 mg/kg). Then an incision was made in the rat abdomen, and the right kidney was removed for nephrectomy. The left kidney was exposed after a midline incision, the renal artery was clamped for 45 min with a nontraumatic clamp, and renal blood flow was then restored."
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M6ACROT02070 "Male BALB/c mice (SJA Laboratory Animal Company, Hunan, China) with age of 6-8 weeks were used in this study to establish COPD model. Mice were housed in individually ventilated cages under a pathogen-free condition, with ad libitum access to food and water. Animal welfare was monitored daily, and all efforts were made to minimize suffering. All animal procedures were conducted in accordance with the guidelines for use of laboratory animals, with approval from the Institutional Animal Care and Use Committee at Jiangxi Provincial People's Hospital (The First Affiliated Hospital of Nanchang Medical College)."
M6ACROT02071 "Male BALB/c mice (SJA Laboratory Animal Company, Hunan, China) with age of 6-8 weeks were used in this study to establish COPD model. Mice were housed in individually ventilated cages under a pathogen-free condition, with ad libitum access to food and water. Animal welfare was monitored daily, and all efforts were made to minimize suffering. All animal procedures were conducted in accordance with the guidelines for use of laboratory animals, with approval from the Institutional Animal Care and Use Committee at Jiangxi Provincial People's Hospital (The First Affiliated Hospital of Nanchang Medical College)."
M6ACROT02072 .
M6ACROT02073 "the left internal carotid artery of the rats was isolated. Then the ligation of middle cerebral artery was performed by a 4/0 surgical nylon monofilament to occlude the blood flow. 2 h later, we removed the filament to restore the blood reperfusion for 24 h. During the entire operation, they were kept at 37.0 ± 0.5 ° C. The laser Doppler flowmetry (Transonic Systems, Ithaca, NY, USA) was applied to confirm the regional ischemia and reperfusion. Rats were decapitated after 24 h of reperfusion."
M6ACROT02074 .
M6ACROT02075 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02076 "All experimental mice were maintained under specific pathogen-free conditions, fed standard laboratory chow, and kept on 12 h light to 12 h dark cycles. The temperature and humidity were set at 22 ± 1 ° C and 55% ± 5%, respectively. Cohoused Cre-negative littermate mice were used as control animals in all experiments."
M6ACROT02077 "All experimental mice were maintained under specific pathogen-free conditions, fed standard laboratory chow, and kept on 12 h light to 12 h dark cycles. The temperature and humidity were set at 22 ± 1 ° C and 55% ± 5%, respectively. Cohoused Cre-negative littermate mice were used as control animals in all experiments."
M6ACROT02078 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02079 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02080 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02081 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02082 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02083 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02084 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02085 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02086 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02087 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02088 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02089 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02090 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02091 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02092 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02093 "Rats were housed at 23 ° C and 55% humidity and were given ad libitum food and water. During acclimatization (1 week), rats were placed randomly (3/cage); however, after initial behavioral testing, they were grouped according to their behavioral phenotypes. All experiments were performed under a light cycle (8:00 AM and 10:00 AM). The protocol to induce LH behavior was approved by the Institutional Animal Care and Use Committee of the University of Alabama at Birmingham. The animal study also adhered to the international guidelines for the use and care of laboratory animals."
M6ACROT02094 "Three strains' mice were used: Shanghai populations of Kunming mice, Tet1fl/fl C57BL/6 mice, and Mettl3fl/fl C57BL/6 mice (Cyagen Biosciences, Guangzhou, China). Pups were kept with their dams after born and weaned at postnatal day 21, then group housed by sex with 4 to 5 mice per cage. The genotype of each mouse was determined by the genomic DNA extracted from tail tip tissue. All mice used in the experiment were 6 8-week-old male mice, maintained at 23 ±3°C with 35% w10% relative humidity, on a 12 hours light/dark cycle (lights on 6:00, lights off 18:00), and libitum accessed to food and water."
M6ACROT02095 "Three strains' mice were used: Shanghai populations of Kunming mice, Tet1fl/fl C57BL/6 mice, and Mettl3fl/fl C57BL/6 mice (Cyagen Biosciences, Guangzhou, China). Pups were kept with their dams after born and weaned at postnatal day 21, then group housed by sex with 4 to 5 mice per cage. The genotype of each mouse was determined by the genomic DNA extracted from tail tip tissue. All mice used in the experiment were 6 8-week-old male mice, maintained at 23 ±3°C with 35% w10% relative humidity, on a 12 hours light/dark cycle (lights on 6:00, lights off 18:00), and libitum accessed to food and water."
M6ACROT02097 "Sprague Dawley rats were anesthetized by intraperitoneal injection of 1.5% pentobarbital sodium at 0.2 mL/100 g. The thoracic aorta was separated under aseptic conditions, and was placed in pre-cooled DMEM medium, and the tunica media was separated under a microscope. The tunica media were cut into small pieces of 0.5-1 mm2, and digested with type II collagenase (2 mg/mL) in a 37°C water bath. The cells were collected by centrifugation, and resuspended in DMEM medium containing 20% FBS. The collected cells were planted in a 6 cm polylysine-coated culture dish, and were cultured in cell incubator for 48 h. The cells were sub-cultured in DMEM medium containing 10% FBS after fusion to 80%. For primary VSMCs stimulated by high glucose, 25 mmol/L glucose (high glucose group) was added and cultured for 24 h for subsequent experiments. primary VSMCs cultured with 5 mmol/L glucose were used as a negative control."
M6ACROT02098 .
M6ACROT02099 .
M6ACROT02100 .
M6ACROT02101 .
M6ACROT02102 .
M6ACROT02103 .
M6ACROT02104 .
M6ACROT02106 "The in vivo tumour growth assays were operated mainly as previously described.18 Briefly, 5 8 × 106 PCDH (empty vector) or 942-OE (transduced with overexpression plasmid) or plvx(empty vector)+shNC, plvx-942-OE+shNC, plvx+shDN3a, 942-OE+shDN3a SGC79001 stably infected cell lines were subcutaneously injected into the dorsal flanks of each mouse (n = 8 or 5). After about 1 week, the xenograft mice were divided into two or four groups stochastically. "
M6ACROT02107 "The in vivo tumour growth assays were operated mainly as previously described.18 Briefly, 5 8 × 106 PCDH (empty vector) or 942-OE (transduced with overexpression plasmid) or plvx(empty vector)+shNC, plvx-942-OE+shNC, plvx+shDN3a, 942-OE+shDN3a SGC79001 stably infected cell lines were subcutaneously injected into the dorsal flanks of each mouse (n = 8 or 5). After about 1 week, the xenograft mice were divided into two or four groups stochastically. "
M6ACROT02108 .
M6ACROT02109 "To assess liver metastasis, indicated cells were intrasplenically injected into mice. At the indicated time, mice were euthanized, and livers were resected, fixed, and subjected to hematoxylin-eosin (HE) staining."
M6ACROT02110 .
M6ACROT02111 SGC-7901 cells stably transfected with shNC or shIGFBP7 were individually resuspended in 100 μL of PBS (5 × 107 cells/ml) and then injected subcutaneously into the nude mice. Tumour volumes and weights were measured every week.
M6ACROT02112 SGC-7901 cells stably transfected with shNC or shIGFBP7 were individually resuspended in 100 μL of PBS (5 × 107 cells/ml) and then injected subcutaneously into the nude mice. Tumour volumes and weights were measured every week.
M6ACROT02113 .
M6ACROT02114 .
M6ACROT02115 .
M6ACROT02116 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02117 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02118 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02119 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02120 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02121 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02122 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02123 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02124 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02125 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02126 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02127 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02128 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02129 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02130 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02131 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02132 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02133 "Female BALB/c nude mice, aged 4-5 weeks, purchased from the Beijing Vital River Laboratory Animal Technology, were allowed to acclimate to local conditions for 1 week and maintained under a 12-h dark/12-h light cycle with food and water provided ad libitum. Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank. When a tumor was palpable, it was measured every other day and the volume was calculated according to the formula volume = length × width2 × 0.5."
M6ACROT02134 .
M6ACROT02135 .
M6ACROT02136 .
M6ACROT02137 .
M6ACROT02138 .
M6ACROT02139 .
M6ACROT02140 .
M6ACROT02141 .
M6ACROT02142 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
M6ACROT02143 .
M6ACROT02145 .
M6ACROT02146 .
M6ACROT02147 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT02148 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT02149 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02150 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02151 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02152 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT02153 .
M6ACROT02154 .
M6ACROT02155 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
M6ACROT02156 .
M6ACROT02158 .
M6ACROT02159 .
M6ACROT02160 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT02161 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT02162 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02163 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02164 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02165 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT02166 .
M6ACROT02167 .
M6ACROT02168 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
M6ACROT02169 .
M6ACROT02171 .
M6ACROT02172 .
M6ACROT02173 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT02174 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT02175 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02176 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02177 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02178 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT02179 .
M6ACROT02180 .
M6ACROT02181 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
M6ACROT02182 .
M6ACROT02184 .
M6ACROT02185 .
M6ACROT02186 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT02187 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT02188 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02189 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02190 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT02191 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT02192 .
M6ACROT02193 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02194 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02195 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02196 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02197 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02198 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02199 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02200 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02201 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02202 "A total of 3 x 106 cells were suspended and mixed with Matrigel (BD Biosciences, Bedford, MA, USA) at a 1:3 volume ratio in 100 μ L of serum-free DMEM and injected subcutaneously into mice. Tumor volumes were measured every 3 days using a caliper and calculated using the standard formula V = shortest diameter2× longest diameter/2. The mice were euthanized when animals exhibited either 30 days after subcutaneous injection or large tumors (volume > 1500 mm3) or obvious signs of discomfort. The tumor was removed, photographed and weighed before being fixed in 10% buffered formalin and analyzed by hematoxylin and eosin (H&E) staining and IHC."
M6ACROT02203 .
M6ACROT02204 .
M6ACROT02205 .
M6ACROT02206 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02207 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02208 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02209 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02210 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02211 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02212 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02213 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02214 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02215 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02216 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02217 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02218 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02219 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02220 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02221 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02222 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02223 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02224 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μ l medium and 50 μ l matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
M6ACROT02225 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02226 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02227 .
M6ACROT02228 .
M6ACROT02229 .
M6ACROT02230 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02231 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02232 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02233 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02234 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02235 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02236 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02237 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02238 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02239 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02240 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02241 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02242 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02243 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02244 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02245 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02246 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02247 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02248 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μ l medium and 50 μ l matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
M6ACROT02249 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02250 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02251 .
M6ACROT02252 .
M6ACROT02253 .
M6ACROT02254 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02255 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02256 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02257 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02258 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02259 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02260 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02261 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02262 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02263 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02264 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02265 "1× 106 MDA-MB-231 cells were resuspended in 100 uL PBS with 50% Matrigel (Corning Costar, USA), and injected into the mammary fat pad of the mice."
M6ACROT02266 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02267 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02268 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02269 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02270 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02271 "Luciferase-labeled rSKBR3 and MDA-MB-361 cells (1 × 107 cells) mixed with 1:1 Matrigel (Corning, 356237) were subcutaneously injected into the fat pads of mice. After a tumor was palpable, the mice were randomized into four groups (five mice per group), and they were treated with vehicle, trastuzumab (20 mg/kg, intraperitoneal administration), roblitinib (30 mg/kg, oral administration), or a combination of both drugs."
M6ACROT02272 "Approximately 1 × 106 viable MDA-MB-231 breast cancer cells were resuspended in 1:1 ratio in 50 μ l medium and 50 μ l matrigel (Corning, 354234) and injected orthotopically into the fourth mammary fat pad of each mouse. After injection, tumor size was measured twice a week using an electronic caliper. Tumor volumes were calculated with the formula: volume = (L × W2)/2, where L is the tumor length and W is the tumor width measured in millimeters."
M6ACROT02273 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02274 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT02275 " Briefly, we co-transduced murine BM progenitor cells (B6.SJL, CD45.1) with the MSCVneo-MLL-AF9 + MSCV-PIG (as control), MSCVneo-MLL-AF9 + MSCV-PIG-miR-550-1 (i.e., MLL-AF9 + miR-550-1), or MSCVneo-MLL-AF9 + MSCV-PIG- miR-550-1 mutant (i.e., MLL-AF9 + miR-550-1 mut) vectors. Transduced cells were then injected into the tail veins of recipient mice (C57BL/6, CD45.2). By flow cytometry, we found all mice in the MLL-AF9+miR-550-1 group displayed an apparent decline in the proportion of c-Kit+ blast cells in the BM, spleen (SP), and PB compared to control or MLL-AF9+miR-550-1 mutant group "
M6ACROT02276 .
M6ACROT03001 .
M6ACROT03002 .
M6ACROT03003 .
M6ACROT03004 .
M6ACROT03005 .
M6ACROT03006 .
M6ACROT03007 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl14-conventional knockout mice. Sex of embryos was determined."
M6ACROT03008 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl15-conventional knockout mice. Sex of embryos was determined."
M6ACROT03009 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl16-conventional knockout mice. Sex of embryos was determined."
M6ACROT03010 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl17-conventional knockout mice. Sex of embryos was determined."
M6ACROT03011 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl18-conventional knockout mice. Sex of embryos was determined."
M6ACROT03012 "Positive ES clones were used for injection into c57 blastocysts and generation of chimerical mice. To produce Mettl14f/+ mice, the chimeras were crossed with wild-type c57 for germ line transmission and then crossed with Atcb-Flpe transgenic mice (The Jackson Laboratory, # 003800) to remove FRT flanked selection cassette. Male Mettl14f/+ mice were crossed to female EIIa-Cre transgenic mice (The Jackson Laboratory, # 003724) to obtain Mettl14+/mice, and Mettl14+/ mice were intercrossed to obtain Mettl19-conventional knockout mice. Sex of embryos was determined."
M6ACROT03013 "Mice were housed in standard conditions in the Laboratory Animal Center of Zhejiang University on a 12 h light/dark schedule with free access to food and water. The pregnant mice were purchased from Shanghai SLAC Laboratory Animal Company, China. All animal experiments were conducted according to protocols approved by the Zhejiang University Animal Care and Use Committee."
M6ACROT03014 .
M6ACROT03015 .
M6ACROT03016 .
M6ACROT03017 .
M6ACROT03018 .
M6ACROT03019 .
M6ACROT03020 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03021 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03022 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03023 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03024 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03025 .
M6ACROT03026 .
M6ACROT03027 .
M6ACROT03028 .
M6ACROT03029 .
M6ACROT03030 .
M6ACROT03031 .
M6ACROT03032 .
M6ACROT03033 .
M6ACROT03034 .
M6ACROT03035 .
M6ACROT03036 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03037 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03038 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03039 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03040 "For tumor xenograft studies, 143B cells stably expressing scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected subcutaneously into the flanks of 4-week-old athymic nude mice (n = 8/group; purchased from Envigo, Inc.). For orthotopic xenograft and experimental metastasis models, 143B cells stably expressing GFP-luc and scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected into tibia or via tail vein, respectively, of 4-week-old athymic nude mice. Equal numbers of male and female mice were used. Tumor volumes and body weight were measured twice a week. Tumor volumes for subcutaneous injection model were measured using caliper and for intratibial and experimental metastasis models using Xenogen in vivo imaging system."
M6ACROT03041 "For tumor xenograft studies, 143B cells stably expressing scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected subcutaneously into the flanks of 4-week-old athymic nude mice (n = 8/group; purchased from Envigo, Inc.). For orthotopic xenograft and experimental metastasis models, 143B cells stably expressing GFP-luc and scrambled shRNA or ALKBH5 shRNA (1 × 106) were injected into tibia or via tail vein, respectively, of 4-week-old athymic nude mice. Equal numbers of male and female mice were used. Tumor volumes and body weight were measured twice a week. Tumor volumes for subcutaneous injection model were measured using caliper and for intratibial and experimental metastasis models using Xenogen in vivo imaging system."
M6ACROT03042 "For the in vivo tumor formation assay, transfected A549 cells (1 × 107) were injected subcutaneously into the right thigh of female BALBc nude mice."
M6ACROT03043 .
M6ACROT03044 .
M6ACROT03045 .
M6ACROT03046 "AGS cells (1 × 107) transfected with the specified material were combined with 50% (v/v) Matrigel (Corning, MA, USA) and subsequently subcutaneously injected into both the left and right dorsal areas of female BALBc nude mice (SPF, Beijing, China). The AGS cells (1 × 107) was subcutaneously injected weekly."
M6ACROT03047 "Briefly, nude mice were anesthetized by intraperitoneal injection of a ketamine (final concentration: 10 mg/mL) and xylazine (final concentration: 1 mg/mL) mixture (0.01 mL/g mouse weight). Then, the mouse sclera was pre-perforated using a sharp 30-gauge injection needle. Ocular melanoma cells (5 × 105) were injected through the hole made in the choroid by a 33-gauge blunt-end microinjection needle (7803-05, Hamilton, Reno, NV, USA). Then, the infected eyes were treated with ophthalmic bacitracin ointment. All animal experiments were approved by the Animal Care and Use Committee at Shanghai Jiao Tong University School of Medicine."
M6ACROT03048 "Briefly, nude mice were anesthetized by intraperitoneal injection of a ketamine (final concentration: 10 mg/mL) and xylazine (final concentration: 1 mg/mL) mixture (0.01 mL/g mouse weight). Then, the mouse sclera was pre-perforated using a sharp 30-gauge injection needle. Ocular melanoma cells (5 × 105) were injected through the hole made in the choroid by a 33-gauge blunt-end microinjection needle (7803-05, Hamilton, Reno, NV, USA). Then, the infected eyes were treated with ophthalmic bacitracin ointment. All animal experiments were approved by the Animal Care and Use Committee at Shanghai Jiao Tong University School of Medicine."
M6ACROT03049 "Briefly, nude mice were anesthetized by intraperitoneal injection of a ketamine (final concentration: 10 mg/mL) and xylazine (final concentration: 1 mg/mL) mixture (0.01 mL/g mouse weight). Then, the mouse sclera was pre-perforated using a sharp 30-gauge injection needle. Ocular melanoma cells (5 × 105) were injected through the hole made in the choroid by a 33-gauge blunt-end microinjection needle (7803-05, Hamilton, Reno, NV, USA). Then, the infected eyes were treated with ophthalmic bacitracin ointment. All animal experiments were approved by the Animal Care and Use Committee at Shanghai Jiao Tong University School of Medicine."
M6ACROT03050 "Briefly, nude mice were anesthetized by intraperitoneal injection of a ketamine (final concentration: 10 mg/mL) and xylazine (final concentration: 1 mg/mL) mixture (0.01 mL/g mouse weight). Then, the mouse sclera was pre-perforated using a sharp 30-gauge injection needle. Ocular melanoma cells (5 × 105) were injected through the hole made in the choroid by a 33-gauge blunt-end microinjection needle (7803-05, Hamilton, Reno, NV, USA). Then, the infected eyes were treated with ophthalmic bacitracin ointment. All animal experiments were approved by the Animal Care and Use Committee at Shanghai Jiao Tong University School of Medicine."
M6ACROT03051 .
M6ACROT03052 .
M6ACROT03053 .
M6ACROT03054 .
M6ACROT03055 .
M6ACROT03056 .
M6ACROT03057 .
M6ACROT03058 .
M6ACROT03059 .
M6ACROT03060 .
M6ACROT03061 .
M6ACROT03062 .
M6ACROT03063 .
M6ACROT03064 .
M6ACROT03065 "About in vivo tumorigenesis model, 1 × 106 indicated SUNE-1 cells were subcutaneously injected into the flanks of nude mice. The tumor volumes were measured every 4 days. On day 32 after injection, the mice were sacrificed and the subcutaneous tumors were excised and weighed. For the inguinal lymph node metastasis model, 2 × 105 indicated SUNE-1 cells were injected into the footpads of nude mice."
M6ACROT03066 "A total of 1 × 107 cotransfected RPMI8226 MM cells, control RPMI8226 MM cells (shNC + LV-NC), ALKBH5-depleted RPMI8226 MM cells (shALKBH5 + LV-NC), or ALKBH5-depleted-SNHG15-overexpressed RPMI8226 MM cells (shALKBH5 + LV-SNHG15), were subcutaneously injected into the right flanks of the 4-wk-old male NOD/SCID mice (n = 6 for each group) to establish a human MM-xenografted model. "
M6ACROT03067 Indicated cells were subcutaneously injected into the back flank of mice. Subcutaneous tumor volumes were measured every week and calculated.
M6ACROT03068 .
M6ACROT03069 "For subcutaneous inoculation, 1 × 106 control or METTL16-depleted CCA cells suspended in 100 μ L PBS mixed with matrix gel (BD, 356,234) at 1:1 ratio was injected into the mice's flanks. Tumor sizes were measured at the relevant time intervals. At the end of the study, the mice were euthanized, and the tumors were dissected and weighed.
For intrahepatic inoculation, 1 × 106 control or METTL16-depleted CCLP1 cells suspended in 10 μ L PBS mixed with matrix gel (BD, 356,234) at a 1:1 ratio was implanted into the livers of NOD-SCID mice. The mice were sacrificed 8 weeks after injection, their livers were surgically dissected, and the liver/body weight ratios were evaluated."
M6ACROT03070 "For subcutaneous inoculation, 1 × 106 control or METTL16-depleted CCA cells suspended in 100 μ L PBS mixed with matrix gel (BD, 356,234) at 1:1 ratio was injected into the mice's flanks. Tumor sizes were measured at the relevant time intervals. At the end of the study, the mice were euthanized, and the tumors were dissected and weighed.
For intrahepatic inoculation, 1 × 106 control or METTL16-depleted CCLP1 cells suspended in 10 μ L PBS mixed with matrix gel (BD, 356,234) at a 1:1 ratio was implanted into the livers of NOD-SCID mice. The mice were sacrificed 8 weeks after injection, their livers were surgically dissected, and the liver/body weight ratios were evaluated."
M6ACROT03071 .
M6ACROT03072 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03073 "PCa cells were resuspended in serum-free RPMI-1640 medium (Gibco, USA) to cell suspension at a density of 1 × 106 cells/200 μL. The Balb/c nude mice were randomly divided into three groups (n = 12 in each group), and the tumorigenesis experiment lasted for 4 weeks. Under anesthesia with ether, the nude mice were disinfected and subcutaneously inoculated with cells transfected with oe-NC, oe-KDM5A + oe-NC and oe-KDM5A + oe-MOB3B at a density of 1 × 106 cells/mouse (200 μL) at the back of the right hind leg."
M6ACROT03074 Hnrnpa2b1fl/fl and Hnrnpa2b1fl/flLyz2-Cre+ mice were infected with 1×108 plaque-forming units (PFU) of HSV-1 viruses intraperitoneally. Serum IFN-beta concentrations were determined by enzyme-linked immunosorbent assay (ELISA) kit. HSV-1 titers were determined by plaque assays using homogenates from brains of infected mice.
M6ACROT03075 Hnrnpa2b1fl/fl and Hnrnpa2b1fl/flLyz2-Cre+ mice were infected with 1×108 plaque-forming units (PFU) of HSV-1 viruses intraperitoneally. Serum IFN-beta concentrations were determined by enzyme-linked immunosorbent assay (ELISA) kit. HSV-1 titers were determined by plaque assays using homogenates from brains of infected mice.
M6ACROT03076 Hnrnpa2b1fl/fl and Hnrnpa2b1fl/flLyz2-Cre+ mice were infected with 1×108 plaque-forming units (PFU) of HSV-1 viruses intraperitoneally. Serum IFN-beta concentrations were determined by enzyme-linked immunosorbent assay (ELISA) kit. HSV-1 titers were determined by plaque assays using homogenates from brains of infected mice.
M6ACROT03077 .
M6ACROT03078 .
M6ACROT03079 .
M6ACROT03080 5 × 106 stable LncRNA-PACERR knockdown (KD) and control THP-1 cell lines treated with PMA and 1 × 107 PATU-8988/0037 wild-type cells were co-injected subcutaneously into per BALB/c nude mice.
M6ACROT03081 .
M6ACROT03082 .
M6ACROT03083 .
M6ACROT03084 .
M6ACROT03085 .
M6ACROT03086 .
M6ACROT03087 .
M6ACROT03088 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT03089 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT03090 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT03091 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT03092 "HepG2 cells overexpressing or knocking out UBR7 were injected subcutaneously into nude mice. The volume of xenograft tumours was measured weekly. After 32 days, the xenograft tumour was taken out and weighed."
M6ACROT03093 .
M6ACROT03094 .
M6ACROT03095 .
M6ACROT03096 .
M6ACROT03097 .
M6ACROT03098 .
M6ACROT03099 .
M6ACROT03100 "The cells were lysed using pyruvate assay buffer, mixed with the reaction mix, and finally detected and measured at 570 nm. All pyruvate production measurements were carried out in triplicate."
M6ACROT03101 "The cells were lysed using pyruvate assay buffer, mixed with the reaction mix, and finally detected and measured at 570 nm. All pyruvate production measurements were carried out in triplicate."
M6ACROT03102 "The cells were lysed using pyruvate assay buffer, mixed with the reaction mix, and finally detected and measured at 570 nm. All pyruvate production measurements were carried out in triplicate."
M6ACROT03103 "The cells were lysed using pyruvate assay buffer, mixed with the reaction mix, and finally detected and measured at 570 nm. All pyruvate production measurements were carried out in triplicate."
M6ACROT03104 .
M6ACROT03105 .
M6ACROT03106 .
M6ACROT03107 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT03108 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT03109 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT03110 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT03111 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT03112 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT03113 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT03114 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT03115 .
M6ACROT03116 .
M6ACROT03117 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice."
M6ACROT03118 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice."
M6ACROT03119 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice."
M6ACROT03120 "Tumor xenograft models were established, with each group consisting of 5 mice (n = 5 per group), including ALKBH5, ALKBH5/shPAK5, NC, shCon, shPAK5, shALKBH5, and shALKBH5. Subcutaneous injections of stably transfected HeLa cells (2.0 × 106 cells) were administered into the armpit region of the nude mice."
M6ACROT03121 .
M6ACROT03122 .
M6ACROT03123 .
M6ACROT03124 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT03125 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT03126 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT03127 "Female BALB/c nude mice (3-4 weeks old) were purchased from Guangdong Medical Animal Experiment Center (Guangzhou, China). Briefly, the indicated HeLa cells (1 × 106) were injected subcutaneously into the right or left armpit of each mouse to establish a tumor xenograft model (n = 10). After two weeks, these mice were euthanized by injecting sodium pentobarbital, and the tumor xenografts were excised and weighed."
M6ACROT03128 Mice were randomly divided into different groups (at least n = 5 each group). H1299-RR cells or A549 cells (5 × 106) treated in different manners were harvested and inoculated subcutaneously into the left dorsal flank of mice.
M6ACROT03129 Mice were randomly divided into different groups (at least n = 5 each group). H1299-RR cells or A549 cells (5 × 106) treated in different manners were harvested and inoculated subcutaneously into the left dorsal flank of mice.
M6ACROT03130 Mice were randomly divided into different groups (at least n = 5 each group). H1299-RR cells or A549 cells (5 × 106) treated in different manners were harvested and inoculated subcutaneously into the left dorsal flank of mice.
M6ACROT03131 .
M6ACROT03132 "A total of 5 × 106 PATU-8988 or organoids derived from PDAC patients (PDO) were orthotopically or subcutaneously injected into per BALB/c nude mice. For the mouse model of CSF1Ri application, 5 × 106 PancO2 cells were orthotopically or subcutaneously injected into per C57BL/6 nude mice."
M6ACROT03133 "A total of 5 × 106 PATU-8988 or organoids derived from PDAC patients (PDO) were orthotopically or subcutaneously injected into per BALB/c nude mice. For the mouse model of CSF1Ri application, 5 × 106 PancO2 cells were orthotopically or subcutaneously injected into per C57BL/6 nude mice."
M6ACROT03134 .
M6ACROT03135 .
M6ACROT03136 .
M6ACROT03137 .
M6ACROT03138 .
M6ACROT03139 .
M6ACROT03140 .
M6ACROT03141 .
M6ACROT03142 .
M6ACROT03143 .
M6ACROT03144 .
M6ACROT03145 .
M6ACROT03146 .
M6ACROT03147 "Female BALB/c nude mice (4 weeks old) were obtained from Beijing SPF (Beijing, China) and were randomly assigned to groups, with each group consisting of 6 mice. To ensure the random allocation of mice into the control and experimental groups, all experimental mice were initially assigned numbers and randomized using a random number table. Consistency was also maintained across factors such as age, environmental conditions, and feeding conditions of the mice. Furthermore, researchers were unaware of the specific group assignment of each mouse, mitigating potential subjective biases. The transfected A549 cells (1 × 107) were then subcutaneously implanted into the flank of each mouse. One month after transplantation, the mice were humanely sacrificed and their tumors were excised for both weighing and histological analysis. The tumor volume was calculated using the formula 1/2 × length × width2. All the mice were housed at the Animal Center of Inner Mongolia University, China, and the study protocol was approved by the Animal Care and Use Committee of Inner Mongolia University (approval ID: IMU-mouse-2022-053)."
M6ACROT03148 "To construct the subcutaneous xenograft model, ~1 × 106 HeLa cells suspended in 50% Matrigel in DMEM were subcutaneously injected into the right flanks of the mice."
M6ACROT03149 .
M6ACROT03150 .
M6ACROT03151 .
M6ACROT03152 .
M6ACROT03153 .
M6ACROT03154 .
M6ACROT03155 .
M6ACROT03156 "Balb/c nude mice were subcutaneously injected with 1 × 106 stable H1299 cells in the lateral abdomen. Tumor size was measured at the indicated times. Eighteen days after implantation of lung cancer cells, 30 mg/kg of Erastin was injected in situ as directed, once every three days. Tumor diameters were recorded at 5-day intervals starting when the tumor was visible and detectable."
M6ACROT03157 "Five-week-old female Balb/c nude mice were purchased from Hunan SJA Laboratory Animal Co., Ltd. For the tumor growth assay, 2 × 106 GC cells were injected subcutaneously into the nude mice, with 5 mice in each group. Tumor volume was measured every 3 days using the following formula: tumor volume = (length × width2)/2. At the end of the experiment, the mice were euthanized, and the tumors were removed, photographed, and weighed. For the in vivo metastasis assay, 1 × 106 GC cells were injected into the nude mice through the tail vein. Four weeks later, the mice were sacrificed, and the metastatic lung tumors were analyzed."
M6ACROT03158 .
M6ACROT03159 The mice were divided into several groups: (a) NC; (b) liver fibrosis model; (c) sh-NC; (d) sh-snail1; (e) adeno-associated virus (AAV)-NC1; (f) AAV-KDM4C; (g) AAV-KDM4C + AAV-NC2; and (h) AAV-KDM4C + AAV-snail1 groups; there were 10 mice in each group.
M6ACROT03160 .
M6ACROT03161 .
M6ACROT03162 "NCG mice were inoculated through the tail vein (day 0) with 0.5 × 106 luciferase-expressing NALM-6 cells, 1 × 106 luciferase-expressing RS4; 11 cells, or 1 × 106 patient-derived B-ALL cells infected with lentivirus expressing shCtrl or shDC1. For EPZ-5676 treatment in vivo, eight-weeks-old female NCG mice were inoculated through the tail vein (day 0) with 0.5 × 106 YTHDC1-overexpressing NALM-6 (DC1highNALM-6) cells or 1 × 106 YTHDC1-overexpressing RS4; 11 (DC1highRS4; 11) cells. "
M6ACROT03163 "NCG mice were inoculated through the tail vein (day 0) with 0.5 × 106 luciferase-expressing NALM-6 cells, 1 × 106 luciferase-expressing RS4; 11 cells, or 1 × 106 patient-derived B-ALL cells infected with lentivirus expressing shCtrl or shDC1. For EPZ-5676 treatment in vivo, eight-weeks-old female NCG mice were inoculated through the tail vein (day 0) with 0.5 × 106 YTHDC1-overexpressing NALM-6 (DC1highNALM-6) cells or 1 × 106 YTHDC1-overexpressing RS4; 11 (DC1highRS4; 11) cells. "
M6ACROT03164 .
M6ACROT03165 .
M6ACROT03166 .
M6ACROT03167 .
M6ACROT03168 .
M6ACROT03169 .
M6ACROT03170 .
M6ACROT03171 .
M6ACROT03172 .
M6ACROT03173 .
M6ACROT03174 .
M6ACROT03175 .
M6ACROT03176 .
M6ACROT03177 .
M6ACROT03178 .
M6ACROT03179 .
M6ACROT03180 .
M6ACROT03181 .
M6ACROT03182 .
M6ACROT03183 .
M6ACROT03184 .
M6ACROT03185 .
M6ACROT03186 "For the inguinal lymph node metastasis model, 3 × 105 SUNE1 cells were injected into the footpads of mice. For the subcutaneous xenograft model, 1 × 106 SUNE1 cells were injected into the axillary epidermis of mice, and tumor size was monitored every day. For the MC38 subcutaneous xenograft model, 1.5 × 106 MC38 cells were injected into the axillary epidermis of C57BL/6J mice, and tumor size was monitored every day."
M6ACROT03187 .
M6ACROT03188 .
M6ACROT03189 .
M6ACROT03190 .
M6ACROT03191 .
M6ACROT03192 "Indicated HCC cells were subcutaneously inoculated into the flank of 5-week-old male BALB/C athymic mice. At the 28th day after inoculation, subcutaneous tumors were resected, weighed, and photographed."
M6ACROT03193 .
M6ACROT03194 .
M6ACROT03195 .
M6ACROT03196 "For xenograft mice model, 1*106 769-P stably transfected cells were subcutaneously injected into the 4-weeks nude mice. For tail vein mice metastasis model, 2*106 769-P stably transfected cells were injected into the 4-weeks nude mice via tail vein. "
M6ACROT03197 "For xenograft mice model, 1*106 769-P stably transfected cells were subcutaneously injected into the 4-weeks nude mice. For tail vein mice metastasis model, 2*106 769-P stably transfected cells were injected into the 4-weeks nude mice via tail vein. "
M6ACROT03198 "To assess in vivo tumor growth, 2 × 106 cells were injected subcutaneously into each mouse and tumors were measured weekly for 6 weeks, after which tumors were resected for weighed and volume."
M6ACROT03199 "To assess in vivo tumor growth, 2 × 106 cells were injected subcutaneously into each mouse and tumors were measured weekly for 6 weeks, after which tumors were resected for weighed and volume."
M6ACROT03200 .
M6ACROT03201 .
M6ACROT03202 .
M6ACROT03203 .
M6ACROT03204 .
M6ACROT03205 .
M6ACROT03206 .
M6ACROT03207 .
M6ACROT03208 Xenograft mouse tumor model was established by inoculating MKN-45 cells transfected with LV Sh355 lentivirus or control LV ShNC lentivirus (2 × 106 in 200 μl sterile 1×phosphate-buffered saline [PBS]) subcutaneously into the right armpit of 5-6-week-old male BALB/c nude mice. GC pulmonary metastatic model was established by injecting MKN-45 cells transfected with LV Sh355 lentivirus or control LV ShNC lentivirus (2 × 106 in 200 μl sterile 1 × PBS) into the caudal vein of 5-6-week-old male BALB/c nude mice
M6ACROT03209 .
M6ACROT03210 .
M6ACROT03211 .
M6ACROT03212 .
M6ACROT03213 "The mice were randomly arranged into six groups according to the bodyweight with 5 mice in each group. HCC cells (5 × 106 cells/mouse) that had been transfected with oe-NC + sh-NC, oe-KDM5B + sh-NC, or oe-KDM5B + sh-ITGA6, or treated with NS, GSK-467 (a selective inhibitor of KDM5B) + oe-NC or GSK-467 + oe-ITGA6 were then subcutaneously implanted into the back of mice. "
M6ACROT03214 .
M6ACROT03215 .
M6ACROT03216 .
M6ACROT03217 .
M6ACROT03218 .
M6ACROT03219 .
M6ACROT03220 .
M6ACROT03221 "Luciferase-labeled A172 cells (1,000,000 cells per mouse) were mixed with polarized macrophages (200,000 cells per mouse), and the mixture was injected into the axilla of nude mice. In detail, two groups of nude mice were injected with pLenti-EZH2-GFP-treated A172 cells and polarized macrophages (n = 8) or pLenti-HK-GFP-treated A172 cells and polarized macrophages. "
M6ACROT03222 .
M6ACROT03223 .
M6ACROT03224 .
M6ACROT03225 .
M6ACROT03226 .
M6ACROT03227 .
M6ACROT03228 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03229 .
M6ACROT03230 .
M6ACROT03231 "For the SNL model, after the animals were anesthetized with isoflurane, an incision on the lower back was made and the lumbar transverse process was removed. The underlying spinal nerve (L4 in mice and L5 in rats) was isolated and ligated with a 3-0 silk thread in rats or 7-0 silk thread in mice. The ligated nerve was then transected distal to the ligature. The skin and muscles were finally closed in layers. For the CCI model, unilateral exposed sciatic nerve was loosely ligated with 3-0 silk thread at four sites with an interval of about 1 mm proximal to trifurcation of the sciatic nerve. "
M6ACROT03232 "For the SNL model, after the animals were anesthetized with isoflurane, an incision on the lower back was made and the lumbar transverse process was removed. The underlying spinal nerve (L4 in mice and L5 in rats) was isolated and ligated with a 3-0 silk thread in rats or 7-0 silk thread in mice. The ligated nerve was then transected distal to the ligature. The skin and muscles were finally closed in layers. For the CCI model, unilateral exposed sciatic nerve was loosely ligated with 3-0 silk thread at four sites with an interval of about 1 mm proximal to trifurcation of the sciatic nerve. "
M6ACROT03233 .
M6ACROT03234 .
M6ACROT03235 .
M6ACROT03236 .
M6ACROT03237 .
M6ACROT03238 .
M6ACROT03239 "About 5 × 106 of MDA-MB-231 sh-NC or sh-METTL3 cells were injected subcutaneously into each 4-week-old immunodeficient BALB/c nude mice. Tumor formation was monitored, and tumor volume was calculated."
M6ACROT03240 "About 5 × 106 of MDA-MB-231 sh-NC or sh-METTL3 cells were injected subcutaneously into each 4-week-old immunodeficient BALB/c nude mice. Tumor formation was monitored, and tumor volume was calculated."
M6ACROT03241 .
M6ACROT03242 .
M6ACROT03243 .
M6ACROT03244 .
M6ACROT03245 .
M6ACROT03246 .
M6ACROT03247 .
M6ACROT03248 .
M6ACROT03249 .
M6ACROT03250 .
M6ACROT03251 .
M6ACROT03252 .
M6ACROT03253 .
M6ACROT03254 .
M6ACROT03255 .
M6ACROT03256 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03257 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03258 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03259 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03260 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03261 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03262 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03263 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03264 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03265 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03266 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03267 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03268 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03269 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03270 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03271 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03272 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03273 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03274 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03275 "C57BL/6J (CD45.2) background Alkbh5fl/fl and Mx1-cre mice were obtained from Biocytogen and the Jackson Laboratory, respectively. B6.SJL (CD45.1) and NOD.Cg-PrkdcscidIL2rgnull (B-NSG) mice were purchased from the Jackson Laboratory and Biocytogen, respectively. All transgenic and knockout mice were CD45.2+. Both male and female mice at 8-10 weeks old were used for experiments, with age- and gender-matched littermates as control. Congenic recipient mice (CD45.2) at 8-10 weeks old were used for AML transplantation, and CD45.1 recipients at 8-10 weeks old were used for normal hematopoietic transplantation assays."
M6ACROT03276 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03277 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03278 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03279 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03280 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03281 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03282 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03283 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03284 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03285 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03286 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03287 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03288 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 2 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03289 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03290 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03291 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03292 "All animal experiments were approved by the animal care Committee of Nanjing First Hospital, Nanjing Medial University (acceptance No. SYXK 20160006). 2 × 106 transfected HCT116 cells in 0.2 ml PBS were injected into the tail vein of nude mice which were randomly divided into nine groups (eight mice per group). After 3 months of injection, mice were sacrificed, and their lungs were removed and stained by Hematoxylin and Eosin (HE) Staining."
M6ACROT03293 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03294 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03295 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03296 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03297 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03298 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03299 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03300 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03301 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03302 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03303 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03304 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03305 "Six-week-old female BALB/c nude mice were purchased from the Model Animal Research Center of Nanjing University and housed in individually ventilated micro-isolator cages under a 12 h dark/light cycle. Nude mice were divided into four groups of six mice each. After deep anesthesia, a 27-gauge needle was used to drill a burrhole into the skull 0.5 mm anterior and 2 mm lateral to the bregma. A 10 μ L gas-tight syringe (Hamilton) was then used to inject 10 μ L of the U87 MG-TMZ_R-luc cell suspension in the striatum at a depth of 3 mm from the dural surface. One week after the injection of the tumor cells, 40 mg/kg/day of TMZ in saline was administered for over 2 weeks by intraperitoneal injection. During TMZ treatment, a Xenogen IVIS Spectrum system was used to monitor the tumor growth. At the end point, brain tissues were dissected from the mice models and measured the length (a) and width (b) of the tumors. Tumor volume was calculated by the formula V = ab2/2."
M6ACROT03306 .
M6ACROT03307 "Twenty BALB/c nude mice (4-6 weeks) were randomly assigned to sh-NC, sh-METTL14, sh-METTL14+oe-NC, and sh-METTL14+oe-PLAGL2, with 5 mice in each group. Mice were raised under sterile conditions of ambient room temperature of 26-28 ° C, the humidity of 40-60%, and alternating day and night for 10 h/14 h. The Mice were fed sterile food and water. After 1 week of adaptive feeding, A549 cells that transfected with sh-NC, sh-METTL14, oe-NC, and oe-PLAGL2 were subcutaneously injected. The cell concentration was 5 × 106/mL, and 200 μ L was injected."
M6ACROT03308 .
M6ACROT03309 "106 cells/100 L NSCLC cell suspension (oe-NC group, oe-METTL14 + oe-NC group, oe-METTL14 + oe-TXNIP group) were injected into the tail vein. Twenty-four nude mice (8 in each group) were employed. Following the 56th post-injection day, mice were decapitated by decortication. Hematoxylin-eosin (HE) staining was used to identify tumor metastases in the mouse lung tissue ."
M6ACROT03310 "About in vivo tumorigenesis model, 1 × 106 indicated SUNE-1 cells were subcutaneously injected into the flanks of nude mice. The tumor volumes were measured every 4 days. On day 32 after injection, the mice were sacrificed and the subcutaneous tumors were excised and weighed. For the inguinal lymph node metastasis model, 2 × 105 indicated SUNE-1 cells were injected into the footpads of nude mice."
M6ACROT03311 .
M6ACROT03312 "METTL3 and TNC overexpression model was achieved by in situ injection of overexpression AAV9 into the heart of C57/BL mice, and the injected virus titers were 1.8 × 1012 PFU/mL. The stable overexpression of METTL3 and overexpression of TNC could be obtained after 3 weeks. The overexpression AAV9 was purchased from Hanheng Biotechnology Co., Ltd. (Shanghai, China). The vector was HBAAV2/9-CMV-m-METTL3-3xflag-Null and HBAAV2/9-CMV-m-TNC-3xflag-Null."
M6ACROT03313 .
M6ACROT03314 .
M6ACROT03315 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03316 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03317 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03318 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03319 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03320 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03321 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03322 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03323 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03324 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03325 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03326 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS."
M6ACROT03327 Stably transfected shMETTL14 and shNC DU145 cells (5× 106 cells) suspended in a mixture of 100uL PBS were subcutaneously injected into the right flank of male nude BALB/C mice (6-8 weeks old) to induce tumor formation.
M6ACROT03328 .
M6ACROT03329 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2× length × 0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
M6ACROT03330 "Approximately 2 × 106 PCa cells (PC-3 shNC, shYTHDF2, shMETTL3 cell lines) per mouse suspended in 100 uL PBS were injected in the flank of male BALB/c nude mice (4 weeks old). During the 40-day observation, the tumor size (V = (width2× length × 0.52)) was measured with vernier caliper. Approximately 1.5 × 106 PCa cells suspended in 100 uL of PBS (PC-3 shNC, shYTHDF2, and shMETTL3 cell lines) per mouse were injected into the tail vein of male BALB/c nude mice (4 weeks old). The IVIS Spectrum animal imaging system (PerkinElmer) was used to evaluate the tumor growth (40 days) and whole metastasis conditions (4 weeks and 6 weeks) with 100 uL XenoLight D-luciferin Potassium Salt (15 mg/ml, Perkin Elmer) per mouse. Mice were anesthetized and then sacrificed for tumors and metastases which were sent for further organ-localized imaging as above, IHC staining and hematoxylin-eosin (H&E) staining."
M6ACROT03331 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
M6ACROT03332 "A total of 1 × 106 PC3 cells or DU145 cells suspended in a mixture of 100 uL PBS and Matrigel were subcutaneously injected into BALB/c nude mice. Tumor weight were measured 2 months after the engraftment. To evaluate the role of METTL3 in tumor metastasis, PC3 cells with or without knockdown of METTL3 were injected into SCID mice through the tail vein (1 × 106 cells per mouse). After eight weeks, mice were sacrificed and their lung tissues were collected for subsequent analyses."
M6ACROT03333 "For liver metastasis model, mice were anaesthetized and an incision was made through the skin and peritoneum to expose the spleen. 1 × 106 HCT116 cells were injected into the spleen (n = 4 each group)."
M6ACROT03334 .
M6ACROT03335 .
M6ACROT03336 .
M6ACROT03337 .
M6ACROT03338 .
M6ACROT03339 .
M6ACROT03340 .
M6ACROT03341 .
M6ACROT03342 .
M6ACROT03343 .
M6ACROT03344 .
M6ACROT03345 .
M6ACROT03346 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03347 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03348 "Equal amount of HCT116 cells (2 × 106) stably expression of relevant plasmids was injected into the right flank of mice, tumor bulks was monitored once a week after injection and volumes were counted as 0.5 × a2 × b (a and b respectively indicated short and long diameter of tumor)."
M6ACROT03349 "For the xenograft model, METTL3 stable overexpressed SW620 cells (1 × 107) or control cells were subcutaneously injected into the right axilla of the female anesthetized BALB/C nude mice (4-6 weeks old, 18-20 g, four mice per group), respectively. The body weight and tumor volumes (length × width2 × 0.5) were measured twice a week. After 21 days, all mice were sacrificed and tumors were surgically removed for hematoxylin-eosin (H&E) staining.For the metastasis model, MTTL3 stable overexpressed SW620 cells (1 × 106) or control cells were injected into the exposed spleen of the anesthetized BALB/C nude mice, respectively. After 21 days, liver metastases were carefully detected using a fluorescent stereoscope and embedded for H&E staining."
M6ACROT03350 .
M6ACROT03351 "For the tumor metastasis mouse model, 5-week-old C57BL/6 mice were randomly grouped and injected with 5 × 105 Control or shFTO stable MC38 cells via tail vein. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days). To detect lung metastasis, mice were killed 2 weeks after tumor cell injection. Lung tissues were harvested and fixed with 4% PFA for paraffin-embedded section and lung metastases were detected with Nikon microscopy. For tumor intraperitoneal mouse model, 2 × 106 Dox-shCtrl, Dox-shFTO stable HCT8/5-FU cells were injected into 5-week-old male BALB/C nude mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or FB23-2 (10 mg/kg every 2 days). For tumor intraperitoneal mouse model, 5 × 105 shCtrl, shFTO-1, shFTO-2 stable MC38 cells were injected into 5-week-old C57BL/6 mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days)."
M6ACROT03352 "Briefly, 4 × 106 transfected HCT116 cells or 8 × 106 transfected SW480 cells in 0.1 mL PBS were injected into mice subcutaneously, and these mice were randomly divided into the experimental and control group."
M6ACROT03353 "Female mice (8 weeks old, 20-25 g) on a C57BL/6J background were fasted for 12 h but were allowed to drink water freely. They were then injected intraperitoneally with 50 mg/kg body weight of freshly dissolved STZ in sterile PBS for four consecutive days. Mice were given sterile PBS alone in the same way as an untreated control. The mice's blood glucose levels were assessed two weeks after their most recent treatment. Mice that exhibited glucose levels greater than 201 mg/dL were classified as successful hyperglycemic models and were utilized in subsequent studies. Five months after the final dose of STZ, the mice were euthanized, and the renal tissues were collected for pathological examination to confirm the successful establishment of the model of diabetes nephropathy induced by hyperglycemia. All animal experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at the China Pharmaceutical University. Isoflurane was used to anesthetize mice."
M6ACROT03354 "Female mice (8 weeks old, 20-25 g) on a C57BL/6J background were fasted for 12 h but were allowed to drink water freely. They were then injected intraperitoneally with 50 mg/kg body weight of freshly dissolved STZ in sterile PBS for four consecutive days. Mice were given sterile PBS alone in the same way as an untreated control. The mice's blood glucose levels were assessed two weeks after their most recent treatment. Mice that exhibited glucose levels greater than 202 mg/dL were classified as successful hyperglycemic models and were utilized in subsequent studies. Five months after the final dose of STZ, the mice were euthanized, and the renal tissues were collected for pathological examination to confirm the successful establishment of the model of diabetes nephropathy induced by hyperglycemia. All animal experiments were performed according to the guidelines of the Institutional Animal Care and Use Committee at the China Pharmaceutical University. Isoflurane was used to anesthetize mice."
M6ACROT03355 "To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times."
M6ACROT03356 .
M6ACROT03357 .
M6ACROT03358 .
M6ACROT03359 .
M6ACROT03360 "3 × 106 CRC cells were inoculated subcutaneously into the right hind leg of BALB/c nude mice. There were 12 nude mice per group. The tumor volume was observed and calculated every 5 days according to the formula: tumor volume = (length × width2)/2. After 30 days, nude mice were euthanatized through intraperitoneal injection of excessive pentobarbital (> 100 mg/kg)."
M6ACROT03361 .
M6ACROT03362 .
M6ACROT03363 "Briefly, 4 × 106 transfected HCT116 cells or 8 × 106 transfected SW480 cells in 0.1 mL PBS were injected into mice subcutaneously, and these mice were randomly divided into the experimental and control group."
M6ACROT03364 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
M6ACROT03365 "For subcutaneous xenotransplantation, 3- to 4-week-old male BALB/c nude mice were randomly divided into groups (8 mice per group) and injected in the back flank with 100 μ L of 1.0 × 107 suspended cells."
M6ACROT03366 "Colorectal cancer cells were seeded in culture plates for 24 h prior to cotransfection with GFP-CARMN, and a vector using Lipofectamine 2000. After 48 h, RNA immunoprecipitation was performed using/Colorectal cancer cells were plated in 24-well plates and incubated for 24 h before cotransfection with the luciferase reporter vector, and the Renilla vector. antibodies against FTO, METTL3 and ALKBH5 from the EZ-Magna RIP Kit (Millipore)."
M6ACROT03367 .
M6ACROT03368 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
M6ACROT03369 .
M6ACROT03370 "6-week old immunodeficient mice (Guangdong Medical Laboratory Animal Center, Guangzhou, China) were selected for generating a subcutaneous xenograft model. MDA-MB-231/DOX cells were implanted subcutaneously into the immunodeficient mice. 7 days later, mice were randomly divided into 4 groups, administrated with vehicle control, FOXO1 inhibitor AS1842856 (20 mg/kg/day, i. p.), Doxorubicin (5 mg/kg/day, i. p.), and AS1842856 combined with Doxorubicin, respectively. Tumor formation was examined every 4 days."
M6ACROT03371 .
M6ACROT03372 "Totally 5 × 106 ESCC cells after treatment were administered to randomized animals by the subcutaneous route (right flank) in 200 uL DMEM. Tumor measurement was performed every other day. At study end (at least 3 weeks), the animals underwent euthanasia, and the tumors were extracted for histology."
M6ACROT03373 "For induction of ESCC, 4-week-old mice were treated with drinking water containing 50 ug/mL 4NQO (Sigma-Aldrich, USA) for 16 weeks and then given normal drinking water for another 4-5 weeks. Cre was activated by the intraperitoneal injection of tamoxifen (Sigma-Aldrich, USA) at a dose of 9 mg per 40 g body weight every other day for a total of three injections. For tumor measurement, mice were sacrificed, and the esophagus was dissected immediately. The surface areas of tumors were measured as described previously."
M6ACROT03374 "Luciferase-labeled KYSE150 cells (5 × 106) were inoculated into the footpads of BALB/c nude mice (4-5 weeks old, 18-20 g) to establish the popliteal lymphatic metastasis model."
M6ACROT03375 "Luciferase-labeled KYSE150 cells (5 × 106) were inoculated into the footpads of BALB/c nude mice (4-5 weeks old, 18-20 g) to establish the popliteal lymphatic metastasis model."
M6ACROT03376 .
M6ACROT03377 .
M6ACROT03378 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth. "
M6ACROT03379 .
M6ACROT03380 .
M6ACROT03381 .
M6ACROT03382 .
M6ACROT03383 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT03384 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT03385 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03386 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03387 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03388 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT03389 .
M6ACROT03390 .
M6ACROT03391 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth. "
M6ACROT03392 .
M6ACROT03393 .
M6ACROT03394 .
M6ACROT03395 .
M6ACROT03396 "Male BALB/c mice were grouped into control, CS, CS+AAV-ShMETTL3, and CS+AAV-NC shRNA groups, n = 6 animals per group. Mice in the AAV-ShMETTL3 and AAV-NC shRNA (GeneChem, China) groups were dosed by intranasal instillation after CS exposure for 4 weeks."
M6ACROT03397 "Four-week-old BALB/c nude mice were randomly divided into three groups: (1) vector group, (2) vector + Bete-elemene group, and (3) Bete-elemene + METTL3 group. Nude mice were raised in an SPF level animal house and were free to eat and drink. Mice in the vector group were subcutaneously injected with lung cancer cells transfected with empty vector and did not receive Bete-elemene administration, and this group was implemented as the negative control. Following establishing orthotopic xenografts by using A549 or H1299 cells transfected with empty vector, mice in the vector + Bete-elemene group underwent intraperitoneal injection with Bete-elemene once a day. For the subcutaneous transplanted model, A549 or H1299 cells transfected with METTL3-overexpressing vector were inoculated into mice from the Bete-elemene + METTL3 group. Then, mice were intraperitoneally administrated with Bete-elemene once a day. Three weeks later, all the animals were euthanized with CO2. Xenografts were removed and weighted after mice were euthanatized."
M6ACROT03398 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03399 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03400 "5 × 106 A549 cells overexpressing METTL3 (Lv-METTL3) or control (Lv-Ctrl) were suspended in 100 uL phosphate-buffered saline (PBS), and were subcutaneously injected into mouse lower right flank. Drug treatment started in the Lv-METTL3 group when the tumour volume reached around 100 mm3. Mice were randomly divided into three groups to receive vehicle, GSK2536771 (30 mg/kg) or rapamycin (1 mg/kg). Drugs were administrated daily through intraperitoneal injection for 18 days. Treatment conditions were chosen as previously reported."
M6ACROT03401 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT03402 .
M6ACROT03403 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT03404 "The animals were maintained in pathogen-free conditions at 21 ° C ± 2 ° C and 55% ± 5% humidity with free access to food and water. Mice were randomly divided into three groups (n = 8 per group) and received a subcutaneous injection of 2 × 106 stably transfected SiHa cells containing the indicated lentivirus (empty, Lv-ALKBH5, Lv-ALKBH5 + Lv-ACC1) diluted in PBS in the left flank. The mice were sacrificed when tumours were apparent on day 30. Tumour volume was recorded 7, 14, 21 and 28 days after injection with a Vernier calliper. After euthanasia, xenografts were excised from mice and weighed."
M6ACROT03405 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT03406 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT03407 "The animals were maintained in pathogen-free conditions at 21 ° C ± 2 ° C and 55% ± 5% humidity with free access to food and water. Mice were randomly divided into three groups (n = 8 per group) and received a subcutaneous injection of 2 × 106 stably transfected SiHa cells containing the indicated lentivirus (empty, Lv-ALKBH5, Lv-ALKBH5 + Lv-ACC1) diluted in PBS in the left flank. The mice were sacrificed when tumours were apparent on day 30. Tumour volume was recorded 7, 14, 21 and 28 days after injection with a Vernier calliper. After euthanasia, xenografts were excised from mice and weighed."
M6ACROT03408 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT03409 5 × 106 of HEP3B and SMMC7721 stable cells were resuspended in 0.1 ml of PBS and subcutaneously injected into the flank of mice.
M6ACROT03410 .
M6ACROT03411 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT03412 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT03413 .
M6ACROT03414 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT03415 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT03416 .
M6ACROT03417 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03418 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03419 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03420 5 × 106 of HEP3B and SMMC7721 stable cells were resuspended in 0.1 ml of PBS and subcutaneously injected into the flank of mice.
M6ACROT03421 .
M6ACROT03422 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT03423 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT03424 .
M6ACROT03425 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT03426 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT03427 .
M6ACROT03428 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03429 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03430 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT03431 "RC cells SW480 at logarithmic growth phase were prepared into cell suspension with a concentration of about 1 × 107/100 L, which was then injected into the left axilla of nude mice with a 1 ml syringe to establish a subcutaneous mouse xenograft model. Once the tumor volume reached about 50 mm3, the nude mice were injected with miR-96 antagomir or NC antagomir (10 nmol once every 5 days for 5 weeks). After 5 weeks, the mice were euthanized, after which the subcutaneous transplanted tumor was removed, and weighed."
M6ACROT03432 .
M6ACROT03433 .
M6ACROT03434 .
M6ACROT03435 .
M6ACROT03436 .
M6ACROT03437 .
M6ACROT03438 .
M6ACROT03439 .
M6ACROT03440 .
M6ACROT03441 .
M6ACROT03442 .
M6ACROT03443 .
M6ACROT03444 "For CDX model, nude mice (female, 4-6-week-old) were subcutaneously injected with 5 × 106 HCT116 cells on the both flank. For PDX model, the patient tumors were divided into small pieces and then inoculated on both flank of nude mice. For knockdown FTO mice model, FTO mice model, two weeks after inoculation, the shFTO#3 lenti-virus injected into the tumor for three consecutive days. For combined medication mice model, intraperitoneal injection of Rhein and Olaparib was started one week after inoculation."
M6ACROT03445 "For CDX model, nude mice (female, 4-6-week-old) were subcutaneously injected with 5 × 106 HCT116 cells on the both flank. For PDX model, the patient tumors were divided into small pieces and then inoculated on both flank of nude mice. For knockdown FTO mice model, FTO mice model, two weeks after inoculation, the shFTO#3 lenti-virus injected into the tumor for three consecutive days. For combined medication mice model, intraperitoneal injection of Rhein and Olaparib was started one week after inoculation."
M6ACROT03446 "Twenty-four specific pathogen free female BALB/c nude mice (age: 6 weeks, weight: 15 ~ 18 g) were purchased from Slac Laboratory Animal Co., Ltd., and subcutaneously injected with SW620 cells stably transfected with oe-NC, oe-GSK3-Beta + oe-NC, or oe-GSK3-Beta + oe-c-Myc to establish a subcutaneous xenograft tumour model in nude mice."
M6ACROT03447 "Twenty-four specific pathogen free female BALB/c nude mice (age: 6 weeks, weight: 15 ~ 18 g) were purchased from Slac Laboratory Animal Co., Ltd., and subcutaneously injected with SW620 cells stably transfected with oe-NC, oe-GSK3-Beta + oe-NC, or oe-GSK3-Beta + oe-c-Myc to establish a subcutaneous xenograft tumour model in nude mice."
M6ACROT03448 FTO-overexpressing and control cells (2 × 106 suspended in 100 ul PBS) were subcutaneously injected into each mouse.
M6ACROT03449 "For the tumor metastasis mouse model, 5-week-old C57BL/6 mice were randomly grouped and injected with 5 × 105 Control or shFTO stable MC38 cells via tail vein. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days). To detect lung metastasis, mice were killed 2 weeks after tumor cell injection. Lung tissues were harvested and fixed with 4% PFA for paraffin-embedded section and lung metastases were detected with Nikon microscopy. For tumor intraperitoneal mouse model, 2 × 106 Dox-shCtrl, Dox-shFTO stable HCT8/5-FU cells were injected into 5-week-old male BALB/C nude mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or FB23-2 (10 mg/kg every 2 days). For tumor intraperitoneal mouse model, 5 × 105 shCtrl, shFTO-1, shFTO-2 stable MC38 cells were injected into 5-week-old C57BL/6 mice. Drug administration was adopted after 48 h. Drug administration (intraperitoneally): DMSO, 5-FU (50 mg/kg every 2 days) or Rhein (10 mg/kg every 2 days)."
M6ACROT03450 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
M6ACROT03451 "To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times."
M6ACROT03452 .
M6ACROT03453 .
M6ACROT03454 .
M6ACROT03455 .
M6ACROT03456 "3 × 106 CRC cells were inoculated subcutaneously into the right hind leg of BALB/c nude mice. There were 12 nude mice per group. The tumor volume was observed and calculated every 5 days according to the formula: tumor volume = (length × width2)/2. After 30 days, nude mice were euthanatized through intraperitoneal injection of excessive pentobarbital (> 100 mg/kg)."
M6ACROT03457 .
M6ACROT03458 .
M6ACROT03459 "Briefly, 4 × 106 transfected HCT116 cells or 8 × 106 transfected SW480 cells in 0.1 mL PBS were injected into mice subcutaneously, and these mice were randomly divided into the experimental and control group."
M6ACROT03460 "For the mouse xenograft model, 2 × 106 cells were injected subcutaneously into the flank regions of female BALB/c nude mice (4-5 weeks)."
M6ACROT03461 "For subcutaneous xenotransplantation, 3- to 4-week-old male BALB/c nude mice were randomly divided into groups (8 mice per group) and injected in the back flank with 100 μ L of 1.0 × 107 suspended cells."
M6ACROT03462 .
M6ACROT03463 "Colorectal cancer cells were seeded in culture plates for 24 h prior to cotransfection with GFP-CARMN, and a vector using Lipofectamine 2000. After 48 h, RNA immunoprecipitation was performed using/Colorectal cancer cells were plated in 24-well plates and incubated for 24 h before cotransfection with the luciferase reporter vector, and the Renilla vector. antibodies against FTO, METTL3 and ALKBH5 from the EZ-Magna RIP Kit (Millipore)."
M6ACROT03464 .
M6ACROT03465 Two hundred milliliters of A549 cells (1 × 106) were injected into the left flank of the back of each mouse.
M6ACROT03466 "LUAD cells stably HCG11 and/or LATS1 overexpressed or silenced were subcutaneously injected into the flank of the BALB/c nude mice (male, 4 weeks old)."
M6ACROT03467 Suspension of H1299 cells (5.0 × 105) was subcutaneously injected into the right flanks of the mice.
M6ACROT03468 "Cells (2 × 104) were seeded onto 12-well plates and cultured in serum-free 1640 medium. Cell spheroids were documented and quantified using an inverted microscope (Olympus, Japan) after two weeks."
M6ACROT03469 .
M6ACROT03470 "After randomly assignment and anesthetization, nude mice were injected with 5 × 106 cells suspended in 100 uL PBS into the tail vein (n = 5 per group). "
M6ACROT03471 "Five-week-old male BALB/c nude mice were used for tumor growth studies in vivo. Briefly, AGS cells were subcutaneously injected into the dorsal side of mice blindly and randomly (n = 5 per group)."
M6ACROT03472 .
M6ACROT03473 "DANCR KO or empty vector control were harvested and then mixed with matrigel (1:1) (BD Biosciences). Three different numbers of cells (1 × 104, 1 × 105, and 5 × 105 cells) were subcutaneously injected into nude mice, five animals per group."
M6ACROT03474 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
M6ACROT03475 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
M6ACROT03476 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
M6ACROT03477 "Nude (nu/nu) mice (4-5 weeks old) were purchased from Harlan Laboratories (Indianapolis, IN, USA). All animal studies were conducted in accordance with NIH animal use guidelines and a protocol approved by the UMMC Animal Care Committee. MIA PaCa-2 cells with LINC00901 overexpression or vector control (pCDH); LINC00901 KO or vector control (LCV2-m) at the exponential stage were harvested and mixed with 50% matrigel, and then injected into mice (2.5 million cells/spot) s.c. as described previously.21 Tumor growth was measured every 4 days, starting 2 weeks after injection of tumor cells."
M6ACROT03478 "To evaluate the effect of IGF2BP2 on the tumor formation ability in vivo, 2 × 106 cells were injected into the axilla of nude mice for subcutaneous tumor formation. After 9 days, modified IGF2BP2-si or si-NC was injected into the tumors every 3 days for 3 weeks. Tumor size was measured every 3 days."
M6ACROT03479 "Male BALB/c nude mice (5-6 weeks) were obtained from Slac Laboratory Animal Center (Shanghai, China) and maintained under pathogen-free conditions. PANC-1 cells (2 × 106 cells suspended in 100 μ l PBS) transfected with circMYO1C knockdown (sh-circMYO1C) or controls (sh-NC) were subcutaneously injected into the flank of nude mice. One week later, the tumor size was measured every three days."
M6ACROT03480 "PANC-1 cells were stably transfected with LV-METTL14, LV-NC, sh-METTL14, sh-NC, or sh-LINC00941. A mixture of 2× 106 cells and 100 μ L PBS was injected into the spleen of every BALB/c nude mouse. After two months of housing in a sterile environment, the mice were sacrificed. Their liver tissues were removed for hematoxylin and eosin (HE) staining."
M6ACROT03481 .
M6ACROT03482 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
M6ACROT03483 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
M6ACROT03484 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
M6ACROT03485 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice."
M6ACROT03486 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT03487 .
M6ACROT03488 "Indicated HCC cells were subcutaneously inoculated into the flank of 5-week-old male BALB/C athymic mice, which were purchased from Shanghai SLAC Laboratory Animal Co. and fed in specific pathogen-free condition. Subcutaneous tumor volumes were measured every week and calculated using the formula 0.5× length× width2. At the 28th day after inoculation, subcutaneous tumors were resected, weighed, and photographed. This study was approved by Affiliated Hospital of Youjiang Medical University for Nationalities Institutional Review Board."
M6ACROT03489 .
M6ACROT03490 .
M6ACROT03491 .
M6ACROT03492 .
M6ACROT03493 The cells (1 × 106) were re-suspended in normal saline and mixed with 25% Matrigel matrix (50 uL) at a 1:1 ratio and subcutaneously injected into the right groin of the mice.
M6ACROT03494 .
M6ACROT03495 .
M6ACROT03496 .
M6ACROT03497 .
M6ACROT03498 .
M6ACROT03499 .
M6ACROT03500 .
M6ACROT03501 "For the purpose of enhancing the overall randomization of the experiment, a random comparison table had been employed. Accordingly, 5-wk-old male nude athymic BALB/c nu/nu mice (Slack, Shanghai, China) were randomly divided into two parts including a control group (NC) and the experimental group METTL14-OE. For developing subcutaneous xeno transplantation model, 5 × 106 HGC-27 cells stably transfected with NC or METTL14 overexpression were subcutaneously incorporated for 5-week-old BALB/c nude mice. The mice experienced euthanasia after 27 days of inoculation and obtained xenografts's mass was obtained. Tumor volume over three days was obtained. To create mouse STAD liver metastasis orthotopic tumor model, 1 × 106 HGC-27 cells under stable transfection with NC or METTL14 overexpression were added to subserosal gastric wall of BALB/c nude mice."
M6ACROT03502 "Male nude mice (age: 4 weeks) were obtained from Charles River (Hangzhou, Zhejiang, China). For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. Next, we detected and measured the tumor volume each week. The weight of the tumor in each nude mouse was also measured at 4 weeks after injection. Immunohistochemistry (IHC) was used to detect Ki67- and caspase-3- positive cells in the tumor. "
M6ACROT03503 "rats were anesthetized with 40 mg/kg sodium pentobarbital. A 21G needle was used to puncture discs 7-8 (Co7-8) from the back through the skin of the tail. The length of the needle was predetermined to ensure a puncture depth of approximately 5 mm. After penetrating the annulus, the needle was rotated 360° and held for 30 seconds to injure the annulus. The sham group underwent the same procedure but without puncture injury to the caudal disc."
M6ACROT03504 Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
M6ACROT03505 A total 5 × 106 stably transfected SiHa cells were subcutaneously injected into the flank of nude mice.
M6ACROT03506 2 × 106 stably transfected HeLa cells were subcutaneously inoculated into the left flank of mice.
M6ACROT03507 Five-week-old male nude BALB/C mice were applied for this animal studies and fed with certified standard diet and tap water ad libitum in a light/dark cycle of 12 h on/12 h off.The assay was performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Stable transfection of METTL3 knockdown (sh-METTL3) or negative control (sh-blank) in SiHa cells (5 × 106 cells per 0.1 mL) were injected into the flank of mice.
M6ACROT03508 "Mice were divided into two groups (n = 4/group) randomly. 3× 106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
M6ACROT03509 "Mice were divided into two groups (n = 4/group) randomly. 3× 106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
M6ACROT03510 .
M6ACROT03511 "Female BALB/c nude mice (4-5 weeks old) were purchased from the Center of Experimental Animals of Guangdong. To establish a tail vein metastasis model, 2 × 106 SiHa cells in 200 μ l PBS were injected into the tail vein of each mouse (n = 6 for both METTL3-overexpressing and empty vector groups). The mice were killed at approximately 8 weeks, and lung tissues were isolated and embedded in paraffin. Hematoxylin and eosin staining was then used to determine the number of lung metastasis nodules. To establish the popliteal lymph node metastasis model, 1 × 106 SiHa cells in 50 μ l PBS were injected subcutaneously into the footpad of each mouse (n = 6 for both groups). Cells from the experimental and control groups were inoculated under the right and left footpads of each mouse, respectively. After 8 weeks, the popliteal lymph nodes were excised."
M6ACROT03512 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
M6ACROT03513 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03514 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03515 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03516 .
M6ACROT03517 Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
M6ACROT03518 A total 5 × 106 stably transfected SiHa cells were subcutaneously injected into the flank of nude mice.
M6ACROT03519 2 × 106 stably transfected HeLa cells were subcutaneously inoculated into the left flank of mice.
M6ACROT03520 Five-week-old male nude BALB/C mice were applied for this animal studies and fed with certified standard diet and tap water ad libitum in a light/dark cycle of 12 h on/12 h off.The assay was performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Stable transfection of METTL3 knockdown (sh-METTL3) or negative control (sh-blank) in SiHa cells (5 × 106 cells per 0.1 mL) were injected into the flank of mice.
M6ACROT03521 "Mice were divided into two groups (n = 4/group) randomly. 3× 106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
M6ACROT03522 "Mice were divided into two groups (n = 4/group) randomly. 3× 106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
M6ACROT03523 .
M6ACROT03524 "Female BALB/c nude mice (4-5 weeks old) were purchased from the Center of Experimental Animals of Guangdong. To establish a tail vein metastasis model, 2 × 106 SiHa cells in 200 μ l PBS were injected into the tail vein of each mouse (n = 6 for both METTL3-overexpressing and empty vector groups). The mice were killed at approximately 8 weeks, and lung tissues were isolated and embedded in paraffin. Hematoxylin and eosin staining was then used to determine the number of lung metastasis nodules. To establish the popliteal lymph node metastasis model, 1 × 106 SiHa cells in 50 μ l PBS were injected subcutaneously into the footpad of each mouse (n = 6 for both groups). Cells from the experimental and control groups were inoculated under the right and left footpads of each mouse, respectively. After 8 weeks, the popliteal lymph nodes were excised."
M6ACROT03525 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
M6ACROT03526 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03527 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03528 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μ l) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μ l HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month."
M6ACROT03529 .
M6ACROT03530 .
M6ACROT03531 .
M6ACROT03532 "HCC cells stably transfected with empty vector or circCPSF6 were subject to construct animal models, followed by the regular treatment of verteporfin, an inhibitor of YAP signaling."
M6ACROT03533 .
M6ACROT03534 .
M6ACROT03535 .
M6ACROT03536 "For the subcutaneous xenograft model, 1 × 106 tumor cells were resuspended in 100 μ L of DMEM and then injected into the axilla of each mouse (n = 5 mice per group). The mice were sacrificed after 2 weeks, the tumor weight was recorded, and the tumor volume was calculated according to the following equation: volume = 1/2*(length × width2). After the experiment, the specimens were fixed with 4% formaldehyde. For the intrahepatic tumor implantation model, 1 × 106 tumor cells were resuspended in 100 μ L of DMEM and then inoculated under the capsule of the right lobe of the liver (n = 5 mice per group). The mice were sacrificed after 4 weeks, and the tumors were removed and subjected to HE staining."
M6ACROT03537 1 × 107 Bel-7404 cells in 200 uL PBS were injected into the right flank of nude mice.
M6ACROT03538 "For mice in each group, 1 × 107 cells in 200 μ L of PBS were injected into the subcutaneous tissue on the right flank. After injection, tumor parameters were measured every 2 days, and the tumor volume was calculated as (length × width × width)/2."
M6ACROT03539 "Luciferase-labeled A172 cells (1,000,000 cells per mouse) were mixed with polarized macrophages (200,000 cells per mouse), and the mixture was injected into the axilla of nude mice. In detail, two groups of nude mice were injected with pLenti-EZH2-GFP-treated A172 cells and polarized macrophages (n = 8) or pLenti-HK-GFP-treated A172 cells and polarized macrophages. "
M6ACROT03540 "Luciferase-labeled A172 cells (1,000,000 cells per mouse) were mixed with polarized macrophages (200,000 cells per mouse), and the mixture was injected into the axilla of nude mice. In detail, two groups of nude mice were injected with pLenti-EZH2-GFP-treated A172 cells and polarized macrophages (n = 8) or pLenti-HK-GFP-treated A172 cells and polarized macrophages. "
M6ACROT03541 "Luciferase-labeled A172 cells (1,000,000 cells per mouse) were mixed with polarized macrophages (200,000 cells per mouse), and the mixture was injected into the axilla of nude mice. In detail, two groups of nude mice were injected with pLenti-EZH2-GFP-treated A172 cells and polarized macrophages (n = 8) or pLenti-HK-GFP-treated A172 cells and polarized macrophages. "
M6ACROT03542 .
M6ACROT03543 .
M6ACROT03544 .
M6ACROT03545 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
M6ACROT03546 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
M6ACROT03547 LoVo cells (5 × 106 cells/ 200 uL PBS) stably transfected with METTL3 knockdown lentiviral vector or control vector were respectively injected subcutaneously into the left flank of each mouse.
M6ACROT03548 .
M6ACROT03549 .
M6ACROT03550 METTL3 stable knockdown or overexpression HCT116 cells were collected and resuspended at a density of 5 × 106 or 3 × 106 cells per 150 uL PBS.
M6ACROT03551 .
M6ACROT03552 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT03553 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT03554 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03555 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03556 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03557 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
M6ACROT03558 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
M6ACROT03559 .
M6ACROT03560 Indicated cells (1 × 107) were subcutaneously injected into 4-week-old male nude mice. Tumor volume was measured every 5 days.
M6ACROT03561 "METTL3 knockout or control CT26 and MC38 cells were injected subcutaneously into the dorsal flank of each 4- to 6-week-old male immunocompetent BALB/c and C57BL/6 mice, respectively. Anti-Gr-1 (BE0075; Bio-X-Cell, Lebanon, NH) or immunoglobulin (Ig)G isotype control (BE0090, Bio-XCell) was given every other day via intraperitoneal injection (150 ug/mouse). SB-265610 (Tocris, Bristol, UK) or phosphate-buffered saline was administrated through intraperitoneal injections at a dosage of 2 mg/kg per day. Tumor sizes were measured every other day. To establish an orthotopic mouse model of CRC, 5- to 6-week-old male C57BL/6 mice were treated with 1.7% dextran sodium sulfate in drinking water for 5 days, and then allowed to recover for 3 days. After 24 hours of fasting, METTL3 knockout or control MC38 cells suspended in 50 ul of 1 mg/mL Matrigel-phosphate-buffered saline (Corning, Corning, NY) were instilled into the colon lumen of anesthetized mice, coated sparingly with Vaseline."
M6ACROT03562 .
M6ACROT03563 "For subcutaneous transplanted model, sh-control and sh-METTL3 HCT-116/5-FU cells (5 × 106 per mouse) were diluted in 100ul PBS + 100 ul Matrigel (BD Biosciences, San Jose, CA, USA) and injected subcutaneously in the rear flank fat pad of the nude mice."
M6ACROT03564 .
M6ACROT03565 "For the tumor xenograft, mice were randomly divided into three groups with four mice for each group. Then, 1 × 106 cells after indicated treatment were harvested and resuspended in 50 ul of PBS. Then the cells were subcutaneously injected into the right front flank of each mouse."
M6ACROT03566 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03567 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03568 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03569 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03570 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03571 "To generate the Mettl3+/- mice, 2 specific single-guide RNAs (sgRNAs) targeting exon 2 of Mettl3 were used, which resulted in a frameshift mutation and generated a premature stop codon."
M6ACROT03572 .
M6ACROT03573 "1 × 106 cells in 100 uL PBS (shMETTL3-1 or shNC) were respectively injected into each mouse through the tail vein. Pulmonary metastases were monitored after fourteen days using the imaging system (IVIS) Spectrum (PerkinElmer, USA)."
M6ACROT03574 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5× 106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
M6ACROT03575 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5× 106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
M6ACROT03576 "HCT-116 cells (3 × 105 cells in 200 uL of saline) were subcutaneously injected into the nude mice to establish xenograft tumors. After 10 days, 10 mg/kg OX or saline was intraperitoneally injected (n = 5 for each group). Si-METTL3 or si-TRAF5 (10 nmol/20 g body weight) was injected twice intratumorally before the start of OX treatment. The mice were examined every 2 days and sacrificed 4 weeks after the OX treatment."
M6ACROT03577 "For the xenograft model, METTL3 stable overexpressed SW620 cells (1 × 107) or control cells were subcutaneously injected into the right axilla of the female anesthetized BALB/C nude mice (4-6 weeks old, 18-20 g, four mice per group), respectively. The body weight and tumor volumes (length × width2 × 0.5) were measured twice a week. After 21 days, all mice were sacrificed and tumors were surgically removed for hematoxylin-eosin (H&E) staining.For the metastasis model, MTTL3 stable overexpressed SW620 cells (1 × 106) or control cells were injected into the exposed spleen of the anesthetized BALB/C nude mice, respectively. After 21 days, liver metastases were carefully detected using a fluorescent stereoscope and embedded for H&E staining."
M6ACROT03578 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
M6ACROT03579 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
M6ACROT03580 "For in vivo lung metastases model, a total of 2 × 106 CRC cells with REG1alpha-overexpression or REG1alpha-knockdown were injected into nude mice via lateral tail vein. Six weeks after injection, the mice were sacrificed and the lung tissues were collected."
M6ACROT03581 "The nude mice were randomly assigned to two groups consisting of six mice each. We injected transformed cells (P0 and P40) into the flank of each mouse in 0.1 mL of sterile PBS to form xenograft tumors. The tumor volume was measured every 2 or 3 days (volume = length × width2 × 1/2). The tumors were resected, imaged, and weighed after the mice were sacrificed. One piece of each tumor tissue was fixed in 4% (v/v) paraformaldehyde for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, and the remaining tissue was stored at 80 ° C."
M6ACROT03582 .
M6ACROT03583 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies."
M6ACROT03584 "oe-STAG3 and sh-METTL3 were transfected into HCT116 cells. Trypsin digestion and cell counting were performed on each set of cells after they had reached around 80% confluence. Nude mice were subcutaneously injected with 200 μ L cells (2 × 106) and randomly assigned to 5 groups (n = 6 in each group): control group (mice were injected with cells without transfection plasmid), oe-NC group (mice were injected with cells transfected with oe-NC), oe-STAG3 group (mice were injected with cells transfected with oe-STAG3), oe-STAG3 + sh-NC group (mice were injected with cells transfected with oe-STAG3 and sh-NC) and oe-STAG3 + sh-METTL3 group."
M6ACROT03585 .
M6ACROT03586 "HCT116 cells stably expressing sh-NC or sh-circ_0124554 were diluted to 2 × 106 cells in 0.2 mL phosphate buffer solution and injected into the mice, followed by 6 Gy irradiation once per day for 5 days. Ten days later, the tumor volume was calculated every 5 days for 5 cycles. Mice were sacrificed after 35 days, and the tumors were dissected for further analysis."
M6ACROT03587 .
M6ACROT03588 .
M6ACROT03589 .
M6ACROT03590 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
M6ACROT03591 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
M6ACROT03592 LoVo cells (5 × 106 cells/ 200 uL PBS) stably transfected with METTL3 knockdown lentiviral vector or control vector were respectively injected subcutaneously into the left flank of each mouse.
M6ACROT03593 .
M6ACROT03594 .
M6ACROT03595 METTL3 stable knockdown or overexpression HCT116 cells were collected and resuspended at a density of 5 × 106 or 3 × 106 cells per 150 uL PBS.
M6ACROT03596 .
M6ACROT03597 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT03598 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT03599 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03600 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03601 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT03602 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
M6ACROT03603 "DLD-1 cells were subcutaneously implanted into 4-6 weeks old female nude mice. When tumors reached a size of about 50 mm3, the nude mice were randomly divided into 6 groups."
M6ACROT03604 .
M6ACROT03605 Indicated cells (1 × 107) were subcutaneously injected into 4-week-old male nude mice. Tumor volume was measured every 5 days.
M6ACROT03606 "METTL3 knockout or control CT26 and MC38 cells were injected subcutaneously into the dorsal flank of each 4- to 6-week-old male immunocompetent BALB/c and C57BL/6 mice, respectively. Anti-Gr-1 (BE0075; Bio-X-Cell, Lebanon, NH) or immunoglobulin (Ig)G isotype control (BE0090, Bio-XCell) was given every other day via intraperitoneal injection (150 ug/mouse). SB-265610 (Tocris, Bristol, UK) or phosphate-buffered saline was administrated through intraperitoneal injections at a dosage of 2 mg/kg per day. Tumor sizes were measured every other day. To establish an orthotopic mouse model of CRC, 5- to 6-week-old male C57BL/6 mice were treated with 1.7% dextran sodium sulfate in drinking water for 5 days, and then allowed to recover for 3 days. After 24 hours of fasting, METTL3 knockout or control MC38 cells suspended in 50 ul of 1 mg/mL Matrigel-phosphate-buffered saline (Corning, Corning, NY) were instilled into the colon lumen of anesthetized mice, coated sparingly with Vaseline."
M6ACROT03607 .
M6ACROT03608 "For subcutaneous transplanted model, sh-control and sh-METTL3 HCT-116/5-FU cells (5 × 106 per mouse) were diluted in 100ul PBS + 100 ul Matrigel (BD Biosciences, San Jose, CA, USA) and injected subcutaneously in the rear flank fat pad of the nude mice."
M6ACROT03609 .
M6ACROT03610 "For the tumor xenograft, mice were randomly divided into three groups with four mice for each group. Then, 1 × 106 cells after indicated treatment were harvested and resuspended in 50 ul of PBS. Then the cells were subcutaneously injected into the right front flank of each mouse."
M6ACROT03611 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03612 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03613 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03614 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03615 "2 × 106 CT26 cells with knockout of Mettl3, Mettl14, Mettl3/Stat1, Mettl3/Irf1, Mettl14/Stat1, or Mettl14/Irf1 and control were suspended in 200 uL of PBS/Matrigel (Corning) (1:1) and then subcutaneously inoculated into flank of each mouse."
M6ACROT03616 "To generate the Mettl3+/- mice, 2 specific single-guide RNAs (sgRNAs) targeting exon 2 of Mettl3 were used, which resulted in a frameshift mutation and generated a premature stop codon."
M6ACROT03617 .
M6ACROT03618 "1 × 106 cells in 100 uL PBS (shMETTL3-1 or shNC) were respectively injected into each mouse through the tail vein. Pulmonary metastases were monitored after fourteen days using the imaging system (IVIS) Spectrum (PerkinElmer, USA)."
M6ACROT03619 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5× 106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
M6ACROT03620 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5× 106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12."
M6ACROT03621 "HCT-116 cells (3 × 105 cells in 200 uL of saline) were subcutaneously injected into the nude mice to establish xenograft tumors. After 10 days, 10 mg/kg OX or saline was intraperitoneally injected (n = 5 for each group). Si-METTL3 or si-TRAF5 (10 nmol/20 g body weight) was injected twice intratumorally before the start of OX treatment. The mice were examined every 2 days and sacrificed 4 weeks after the OX treatment."
M6ACROT03622 "For the xenograft model, METTL3 stable overexpressed SW620 cells (1 × 107) or control cells were subcutaneously injected into the right axilla of the female anesthetized BALB/C nude mice (4-6 weeks old, 18-20 g, four mice per group), respectively. The body weight and tumor volumes (length × width2 × 0.5) were measured twice a week. After 21 days, all mice were sacrificed and tumors were surgically removed for hematoxylin-eosin (H&E) staining.For the metastasis model, MTTL3 stable overexpressed SW620 cells (1 × 106) or control cells were injected into the exposed spleen of the anesthetized BALB/C nude mice, respectively. After 21 days, liver metastases were carefully detected using a fluorescent stereoscope and embedded for H&E staining."
M6ACROT03623 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
M6ACROT03624 "A total of 8 × 106 wild-type (WT) or METTL3-knockdown cells were injected into the dorsal flanks of 6-week-old nude mice. Seven mice were randomly selected to calculate the volume according to the following formula: V = (width2 × length)/2. Mice were euthanized three weeks after injection and tumors removed, weighed, fixed, and embedded for immunohistochemical analysis."
M6ACROT03625 "For in vivo lung metastases model, a total of 2 × 106 CRC cells with REG1alpha-overexpression or REG1alpha-knockdown were injected into nude mice via lateral tail vein. Six weeks after injection, the mice were sacrificed and the lung tissues were collected."
M6ACROT03626 "The nude mice were randomly assigned to two groups consisting of six mice each. We injected transformed cells (P0 and P40) into the flank of each mouse in 0.1 mL of sterile PBS to form xenograft tumors. The tumor volume was measured every 2 or 3 days (volume = length × width2 × 1/2). The tumors were resected, imaged, and weighed after the mice were sacrificed. One piece of each tumor tissue was fixed in 4% (v/v) paraformaldehyde for hematoxylin and eosin (H&E) and immunohistochemistry (IHC) staining, and the remaining tissue was stored at 80 ° C."
M6ACROT03627 .
M6ACROT03628 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies."
M6ACROT03629 "oe-STAG3 and sh-METTL3 were transfected into HCT116 cells. Trypsin digestion and cell counting were performed on each set of cells after they had reached around 80% confluence. Nude mice were subcutaneously injected with 200 μ L cells (2 × 106) and randomly assigned to 5 groups (n = 6 in each group): control group (mice were injected with cells without transfection plasmid), oe-NC group (mice were injected with cells transfected with oe-NC), oe-STAG3 group (mice were injected with cells transfected with oe-STAG3), oe-STAG3 + sh-NC group (mice were injected with cells transfected with oe-STAG3 and sh-NC) and oe-STAG3 + sh-METTL3 group."
M6ACROT03630 .
M6ACROT03631 "HCT116 cells stably expressing sh-NC or sh-circ_0124554 were diluted to 2 × 106 cells in 0.2 mL phosphate buffer solution and injected into the mice, followed by 6 Gy irradiation once per day for 5 days. Ten days later, the tumor volume was calculated every 5 days for 5 cycles. Mice were sacrificed after 35 days, and the tumors were dissected for further analysis."
M6ACROT03632 "Animals were bred and housed in the pathogen-free facility of the Laboratory Animal Center of Shanghai General Hospital (Shanghai, China). All lungs were collected 4 weeks after BLM treatment for histology and further study. Lung microsections (5 uM) were applied to Masson's trichrome and Sirius red staining to visualize fibrotic lesions."
M6ACROT03633 .
M6ACROT03634 .
M6ACROT03635 .
M6ACROT03636 .
M6ACROT03637 .
M6ACROT03638 .
M6ACROT03639 .
M6ACROT03640 .
M6ACROT03641 .
M6ACROT03642 .
M6ACROT03643 .
M6ACROT03644 .
M6ACROT03645 Mice 8 weeks after splenic portal vein injection of BGC823 cells with METTL3 overexpression or vector-transfected cells.
M6ACROT03646 The luciferase signal intensity from days 7 to 42 is on equivalent scales in the models. Bioluminescent flux (photons/s/cm2/steradian) was determined for the lung metastases.
M6ACROT03647 .
M6ACROT03648 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
M6ACROT03649 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
M6ACROT03650 A total of 2 × 106 GC cells were injected into the flank of nude mice in a 1:1 suspension of BD Matrigel (BD Biosciences) in phosphate-buffered saline (PBS) solution.
M6ACROT03651 The GC cell line MKN-45 stably infected with lentivirus expressing sh-HBXIP was prepared into 5 × 107 cells/mL cell suspension.
M6ACROT03652 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03653 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03654 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03655 "For the formation of xenograft tumors, 5 × 106 AGS cells mixed in Matrigel (BD Biosciences, Franklin Lakes, NJ, USA) were subcutaneously injected into BALB/c nude mice (5-week-old male)."
M6ACROT03656 "100,000 pLKO and PARP1-sh1 (PT1 and PT2) cells were mixed with matrix gel and inoculate into BALB/C nude mice, respectively. After 25 days, 6 organoid transplanted tumor mice were treated with oxaliplatin (Sellekchem, s1224) twice a week for 4 weeks at a dose of 5 mg/kg."
M6ACROT03657 "For subcutaneous xenograft models, 0.1 mL of cell suspension containing 106 cells were injected subcutaneously into the right flank of mice (n = 6 for each group)."
M6ACROT03658 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03659 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03660 "The GC cell line MKN-45 stably infected with lentivirus expressing sh-HBXIP was prepared into 5 × 107 cells/mL cell suspension. The cell suspension was injected into the left axilla of nude mice using a 1 mL syringe as the sh-HBXIP group (n = 6). The GC cell line MKN-45 infected with the lentivirus expressing sh-NC was dispersed into the cell suspension, which was injected into nude mice as the sh-NC group (n = 6). Tumor growth was observed and data were recorded after inoculation. On the 26th day, all nude mice were euthanized by cervical dislocation and the tumors were resected and weighed."
M6ACROT03661 "A total of 5 × 106 stably transfected HGC-27 cells were subcutaneously injected into the right axillary fossa of nude mice. Tumor volume was measured every 3 days and calculated with the following formula: V = (L × W2)/2 cm2 (V, tumor volume; L, length; W, width). The mice were sacrificed at 3-4 weeks after injection, and the tumors were weighed. For the lung metastasis model, 5 × 106 stably transfected HGC-27 cells were injected into the tail veins of nude mice. Forty-five days later, the mice were sacrificed, and the lungs were dissected to examine the histopathological metastatic loci. The peritoneal dissemination ability of GC cells was evaluated via intraperitoneal injection. A total of 5 × 106 stably transfected HGC-27 cells in 500 uL of PBS were injected into the peritoneal cavity of BALB/c nude mice. Mice were carefully monitored until they were killed at 4 weeks, at which point peritoneal metastases were examined and recorded."
M6ACROT03662 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03663 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1."
M6ACROT03664 "The nude mice were inoculated in the inguinal region subcutaneously with 1 × 106 stably transfected GC cells which were suspended in 100 μ L PBS. After one week, preestablished tumor xenografts were treated with DMSO or OTS186935 (50 mg/kg, 2 × /wk × 3)."
M6ACROT03665 "Male nude mice (age: 4 weeks) were obtained from Charles River (Hangzhou, Zhejiang, China). For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. Next, we detected and measured the tumor volume each week. The weight of the tumor in each nude mouse was also measured at 4 weeks after injection. Immunohistochemistry (IHC) was used to detect Ki67- and caspase-3- positive cells in the tumor."
M6ACROT03666 "Stably transfected GC cells (1 × 106) with sh-CENPF or CENPF overexpression were mixed in 0.1 mL PBS, and then were injected subcutaneously into the groin of 5-week-old BALB/C nude mice (n = 5 each group). After four weeks, the nude mice were sacrificed, and tumor tissues were dissected. Finally, the weight and volume of the tumors were measured. Tumor volume = length × width2 × 1/2.A total of 1 × 106 luciferase labeled GC cells were injected into the spleen of 5-week-old BALB/C nude mice. After 4-5 weeks, bioluminescence signals of liver metastases were detected via an IVIS imaging system (PerkinElmer, Norwalk, Connecticut, USA). Liver tissues were removed for hematoxylin-eosin (HE) (Beyotime, Shanghai, China) stained sections to evaluate metastatic liver lesions."
M6ACROT03667 "Twenty nude mice were used to establish the transfer model. KLHL5 or normal SGC-7901 cells were knocked out using luciferase labeling (Genechem, China) and injected through the tail vein to construct tumor models. After 3 weeks, the tumor growth in mice was observed by small animal imaging technology. After the mice were euthanized, lung tissue was taken to observe tumor metastasis. In addition, eight nude mice were subcutaneously implanted with high/low KLHL5-expressing mass tissues from human subjects to evaluate the effect of KLHL5 on xenograft tumor proliferation."
M6ACROT05001 .
M6ACROT05002 Four-week-old immunodeficient BALB/c female nude mice were randomly divided into two groups (n = six for each group). MGC-803 cells (2 × 107) with stable sh-SNHG12 or empty vector were separately subcutaneously injected into the flanks of the subjects.
M6ACROT05003 .
M6ACROT05004 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05005 The division of 6-week-old BALB/c nude mice was done in two groups (five mice per group) randomly. The 786-O cells that were stably transfected with shCtrl or shLINC01426 (6 × 106 cells per mouse) were subcutaneously injected into the right flanks of mice.
M6ACROT05006 .
M6ACROT05007 "Mice were randomly divided into control, sh-NC, and sh-RMRP groups by a researcher who was blinded to our experimental design. Each group contains 6 mice. U251/TMZ cells (1 × 107 cells/mouse), U251/TMZ cells infected with sh-NC (1 × 107 cells/mouse), or U251/TMZ cells infected with sh-RMRP (1 × 107 cells/mouse) were injected subcutaneously into the right-back of mice in the control, sh-NC, or sh-RMRP group, respectively. TMZ (25 mg/kg body weight) was intraperitoneally injected into mice every other day for 14 days when tumor volumes reached approximately 100 mm3."
M6ACROT05008 "Control or NBAT1 overexpressing Hep3B cells (107 cells/mouse, n = 5 per group) were injected subcutaneously into the the flanks of BALB/c nude mice. Their tumor growth was monitored every 5 days. The tumor volumes were calculated by the formula 0.5 × width2 × length. After 35 days, the mice were sacrificed, and the tumors were isolated and weighed."
M6ACROT05009 Mice were bred and maintained in a pathogen-free facility until the age of 6-week. 1 × 10^6 H19 overexpressed or control cells were suspended in 100 μL PBS and injected into the tail vein.
M6ACROT05010 .
M6ACROT05011 .
M6ACROT05012 .
M6ACROT05013 .
M6ACROT05014 .
M6ACROT05015 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05016 2 × 106 stably transfected HeLa cells were subcutaneously inoculated into the left flank of mice.
M6ACROT05017 .
M6ACROT05018 .
M6ACROT05019 .
M6ACROT05020 "For the tumorigenicity studies, control shRNA or sh-LINC01234 stably expressed A549 cells (3 × 106) were injected subcutaneously into the ventral side of male BALB/c nude mice (4 weeks old)."
M6ACROT05021 .
M6ACROT05022 .
M6ACROT05023 "As previously described, Xenograft in vivo mice assay was performed.13 Transfected MDA-MB-436 or MDA-MB-453 cells with sh-ZEB1-AS1#1 or NC (sh-NC) were subcutaneously injected into mice. Every 3 days, tumor growth was monitored."
M6ACROT05024 .
M6ACROT05025 .
M6ACROT05026 Harvested cells were resuspended in PBS and each side of mouse was injected about 1 × 106 cells. Tumor volume was estimated every four days and calculated as 0.5 × length × width2.
M6ACROT05027 Harvested cells were resuspended in PBS and each side of mouse was injected about 1 × 106 cells. Tumor volume was estimated every four days and calculated as 0.5 × length × width2.
M6ACROT05028 .
M6ACROT05029 .
M6ACROT05030 .
M6ACROT05031 .
M6ACROT05032 .
M6ACROT05033 .
M6ACROT05034 .
M6ACROT05035 .
M6ACROT05036 .
M6ACROT05037 5 × 106 of HEP3B and SMMC7721 stable cells were resuspended in 0.1 ml of PBS and subcutaneously injected into the flank of mice.
M6ACROT05038 .
M6ACROT05039 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT05040 A number of 5 × 106 SMMC7721 or MHCC97H cells re-suspended in 100 uL of PBS were subcutaneously injected into the right flank of 6-week old male NCG mice.
M6ACROT05041 .
M6ACROT05042 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT05043 "For lung metastasis model, 1 × 106 HCC cells suspended in 100 ul serum free DMEM were injected via the tail vein of nude mice."
M6ACROT05044 .
M6ACROT05045 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μ L DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT05046 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μ L DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT05047 "Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained from Vital River Laboratory Animal Technology (Beijing, China). MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μ L DMEM were subcutaneously or orthotopically implanted into the nude mice."
M6ACROT05048 Each group contained 15 mice. Mice were injected subcutaneously with 3×105 cells in the right axillary fossa. Tumor size was measured every five days until 60 days after injection
M6ACROT05049 "200 μ L PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT05050 "200 μ L PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT05051 .
M6ACROT05052 5 × 106 cells were subcutaneously injected into the left or right flank of each mouse.
M6ACROT05053 .
M6ACROT05054 .
M6ACROT05055 "For the orthotopic models, 2 × 106 cells with negative control (NC, sh-NC), sh-1 or sh-2 in 0.5 mL of PBS were subcutaneously injected into the dorsal flank of 2 mice respectively. After the tumors grew up to 1 cm3, they were resected and equally divided into small pieces. Then 15 mice were separated into 3 groups (sh-NC, sh-1 and sh-2), of which the tumor pieces were tied to the base of the ceca."
M6ACROT05056 "For the animal survival analysis, mice were intracranially injected with 10,000 GSCs and maintained until moribund or 80 days after injection. For the rescue studies, GSCs with ALKBH5 or FOXM1-AS shRNAs were co-transfected with a FOXM1, ALKBH5 wild-type or mutant expression construct. A total of 50,000 GSCs were intracranially injected into mice (n = 8 mice per group). "
M6ACROT05057 .
M6ACROT05058 "NOD-SCID mice in each experimental group were injected with 2 × 106 HCT-116-Luc-LINC00266-1-ORF-Flag, HCT-116-Luc-LINC00266-1-5'U, or HCT-116-Luc-LINC00266-1-MT cells through the tail vein (n = 5). The metastatic foci were visualized 9 weeks after implantation using an IVIS 200 Imaging System (Xenogen)."
M6ACROT05059 .
M6ACROT05060 "In the CRISPR/Cas9-generated HNF4a knockout (HNF4a-/-) mouse models, the size and weight of the liver were lower compared to those of the wild-type mice. Moreover, the expression of circ_104075 in the liver of the HNF4a-/- mice was lower than that in the wild-type mice. We also determined the level of the HNF4a mRNA in clinical liver tissues, and found that the HNF4a mRNA level was higher in the HCC tissues than in adjacent normal tissues."
M6ACROT05061 .
M6ACROT05062 .
M6ACROT05063 .
M6ACROT05064 .
M6ACROT05065 "Female BALB/c nude mice aged 4 weeks old were adopted. In the subcutaneous xenograft tumor, GC cells (2 × 106/200 μ l) were subcutaneously injected into the mice flank."
M6ACROT05066 .
M6ACROT05067 .
M6ACROT05068 .
M6ACROT05069 .
M6ACROT05070 .
M6ACROT05071 .
M6ACROT05072 .
M6ACROT05073 Subcutaneous xenograft model in nude mice: Each group of HeLa cells was prepared as a cell suspension at a concentration of 2 × 107/mL.
M6ACROT05074 .
M6ACROT05075 .
M6ACROT05076 .
M6ACROT05077 .
M6ACROT05078 "Ten mice were randomly divided into two groups, and C4-2-pZXE-circDDIT4 or C4-2-pZXE-NC cell suspension was concentrated to 5 × 106 cells/100 μ L PBS and then injected into the flanks of the nude mice. After 20 days, the mice were euthanized, and the xenograft tumors were harvested, weighed, and photographed."
M6ACROT05079 .
M6ACROT05080 .
M6ACROT05081 .
M6ACROT05082 .
M6ACROT05083 .
M6ACROT05084 .
M6ACROT05085 "For the subcutaneous tumor growth assay, 6-8 weeks old nude mice were sorted into six groups (n = 5 per group) at random. Each group received one of the following treatments bilaterally into the subcutaneous tissue of the flank: MHCC97H cells alone (1× 10^6), exosomes from M0 macrophages incubated with MHCC97H cells (1× 10^6), MHCC97H with AS1-KD exosome-treated M0 macrophages (1× 10^6), and MHCC97H coupled with exosomes from siNC treated M2 macrophages (1× 10^6) or siIL-6 treated M2 macrophages (1× 10^6)."
M6ACROT05086 .
M6ACROT05087 .
M6ACROT05088 .
M6ACROT05089 .
M6ACROT05090 .
M6ACROT05091 .
M6ACROT05092 .
M6ACROT05093 .
M6ACROT05094 .
M6ACROT05095 .
M6ACROT05096 .
M6ACROT05097 .
M6ACROT05098 "Ten BALB/C nude mice (4 weeks old, female) were injected in 1 × 106 HCT116 cells in 100 uL PBS at each side. The tumor size was detected every four days after the injection of cells and calculated according to the formula."
M6ACROT05099 "Adult C57BL/6 J mice (N = 40, 24-26 g) were used for animal experiments. Mice were randomly divided into 2 groups: sham (N = 10) and MCAO/R (N = 30). MCAO/R model mice were randomly divided into three groups: MCAO/R, MCAO/R + Exo-shNC, and MCAO/R + Exo-shKLF4, and mice were injected with Exo-shNC and Exo-shKLF4 through the tail vein (the injection concentration was 100 μg exosomes in 100 L PBS according to the previous study)."
M6ACROT05100 .
M6ACROT05101 .
M6ACROT05102 .
M6ACROT05103 "Mice were injected with Molm13-luc-GFP AML cells treated with sh-NC, sh-MEG3, sh-NC + AraC, sh-MEG3 + AraC, oe-NC, oe-MEG3, oe-NC + AraC, or oe-MEG3 + AraC, respectively (n = 8 in each group).NSG mice were intravenously injected with cells treated by Molm13-luc-GFP or Molm13 R-luc-GFP (2 × 106 cells/100 μ L)."
M6ACROT05104 "Mice were injected with Molm13-luc-GFP AML cells treated with sh-NC, sh-MEG3, sh-NC + AraC, sh-MEG3 + AraC, oe-NC, oe-MEG3, oe-NC + AraC, or oe-MEG3 + AraC, respectively (n = 8 in each group).NSG mice were intravenously injected with cells treated by Molm13-luc-GFP or Molm13 R-luc-GFP (2 × 106 cells/100 μ L)."
M6ACROT05105 .
M6ACROT05106 "In order to develop a xenograft model, 12 nude mice (1 × 107cells per 200 μ L of FBS-free medium) were injected subcutaneously with CAOV3 cells."
M6ACROT05107 7 × 106 SGC7901 cells stably overexpressed LNC942 or pCDH empty vector (LNC942/NC) were suspended in 125-μ l PBS and inoculated into the right dorsal flank of 5-week-old female nude mice (n = 5 per group).
M6ACROT05108 "a total of 5 × 105 U87MG cells stably expressing firefly luciferase (Fluc) with indicated treatments were injected into the mouse brain at 2 mm lateral, 2 mm posterior to the bregma, and 2 mm depth via a stereotaxic apparatus."
M6ACROT05109 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 5 per group). A549 cells (2 × 106) that had been stably transfected with sh-LCAT1 or scramble were implanted subcutaneously into the nude mice."
M6ACROT05110 Stable cells were selected with 2 μ g/ml puromycin for two weeks. Male athymic BALB/c nude mice (4-5 weeks old) were used. Subcutaneous tumor growth assays were used for growth assay. A total of 5 × 106 cells was subcutaneously injected into the nude mice (n = 3). The tumor volume was measured. The mice were sacrificed 4 weeks after injection. The tumor tissues were isolated and weighed. A tail vein injection model was used for metastasis assays. A total of 5 × 105 cells were injected into tail vein of nude mice (n = 3).
M6ACROT05111 "For tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
M6ACROT05112 "For tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
M6ACROT05113 "For tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
M6ACROT05114 "For tumor growth assessment, 104 Huh7/CSC infected with different lentiviral vectors were mixed with Matrigel at a 1:1 ratio, and the mixture was injected subcutaneously into nude mice (n = 6). For mouse survival assessment, a mixture of 105 infected Huh7/CSC cells with Matrigel was administered to the liver of nude mice."
M6ACROT05115 "Approximately 2 × 106 NSCLC cells (A549), transfected with sh-DLGAP1-AS2 or controls, were suspended in 100 μ l PBS and then injected into flank of male BALB/c nude mice (5-wk old)."
M6ACROT05116 "Six-week-old male nude BALB/C mice were randomly divided into two groups, NC and sh-HCP5#1 groups. 2 × 106 EC109 cells were subcutaneously injected into nude mice and grown for 5 weeks."
M6ACROT05117 "shRNA control (empty vector) and sh-H19 were transfected into BGC-823 cells for 24 h. The cells were collected after digested by trypsin. The cells were washed using phosphate buffered saline (PBS) (Gibco, USA) twice and resuspended with PBS before being subcutaneously injected into nude mice."
M6ACROT05118 .
M6ACROT05119 "The mice were subcutaneously inoculated with 6 × 107 HepG2 cells stably transfected with pLV-circGPR137B and empty vectors or Sk-hep-1 cells stably transfected with pLV-sh-circGPR137B/sh-NC. HepG2 cells stably transfected with circGPR137B or pcDNA3.1 were cultured in complete medium. When the cells were 70% confluent, the medium was replaced with fresh medium to remove dead and detached cells. Subsequently, 6 × 107 cells were used to inject into mice via the tail vein. Each mouse was injected with 100ul (6× 106 cells)."
M6ACROT05120 "The mice were subcutaneously inoculated with 6 × 107 HepG2 cells stably transfected with pLV-circGPR137B and empty vectors or Sk-hep-1 cells stably transfected with pLV-sh-circGPR137B/sh-NC. HepG2 cells stably transfected with circGPR137B or pcDNA3.1 were cultured in complete medium. When the cells were 70% confluent, the medium was replaced with fresh medium to remove dead and detached cells. Subsequently, 6 × 107 cells were used to inject into mice via the tail vein. Each mouse was injected with 100ul (6× 106 cells)."
M6ACROT05121 "Male BALb/c nude mice (three mice per group) were bought from Guangdong Medical Laboratory Animal Center, and indicated HCC cells were subcutaneously injected into the flanks of nude mice at 2 × 106 cells per site."
M6ACROT05122 "4-6 weeks old male BALB/c nude mice were used in present study. Lymph node metastasis animal model was established by injecting 2 × 105 RPRD1B-overexpressed HGC27 and shRPRD1B-transfected AGS cells into right hind footpad of nude mice, respectively."
M6ACROT05123 .
M6ACROT05124 "Male C57BL/6J mice (6-8 weeks old and 18-21 g weight) were randomly divided into 3 groups, including ctrl + ad-NC, LPS + ad-NC and LPS + ad-sh-Mir22hg."
M6ACROT05125 "The AMI model was induced by permanent ligation of the left coronary artery anterior descending with a 7-0 surgical suture as previously described , and then miR-636 antagomir (10 mg/kg; RiboBio) was injected through the tail vein of mice or the nanoparticles with DKK2 plasmids were administered intraperitoneally in a volume of 200 μ L into AMI mice."
M6ACROT05126 "A total of 5× 106 SK-Hep-1 cells (shNC, sh1089-1, or sh1089-2) into fossa axillaries of 5-week-old male nude BALB/c mice (Vital River Laboratory; n = 5 per group).in vivo, we injected a total of 2× 107 SK-Hep-1 cells with stable LINC01089-KD (shNC, sh1089-1, or sh1089-2) or LINC01089-OE (NC or lnc1089) into the middle of the lower abdomen of male nude BALB/c mice (Vital River Laboratory; n = 6 per group).To investigate the involvement of LINC01089 in hematogenous metastases, a total of 1× 107 SK-Hep-1 cells with stable firefly luciferase expression (shNC, sh1089-1, sh1089-2, NC, or lnc1089) were injected into tail vein of male nude BALB/c mice (Vital River Laboratory; n = 4 per group)."
M6ACROT05127 .
M6ACROT05128 "Experiment 1:Eighteen rats were allocated to Control, DN, and DN + NAC (an inhibitor of ROS) groups (n = 6/group) at random. Rats in the DN and DN + NAC groups were subjected to high-fat diets (45% kcal fat), whereas control rats were normally bred with 10% kcal fat. Experiment 2:Twelve rats were randomly divided into DN + PBS and DN + Exo groups (n = 6/group). Experiment 3:Twenty-four rats were randomly assigned to DN, DN + Exo, DN + Exo-mimic NC, and DN + Exo-miR-204 mimic groups (n = 6/group)."
M6ACROT05129 .
M6ACROT05130 .
M6ACROT05131 .
M6ACROT05132 .
M6ACROT05133 .
M6ACROT05134 .
M6ACROT05135 .
M6ACROT05136 .
M6ACROT05137 .
M6ACROT05138 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05139 .
M6ACROT05140 .
M6ACROT05141 .
M6ACROT05142 .
M6ACROT05143 .
M6ACROT05144 .
M6ACROT05145 .
M6ACROT05146 .
M6ACROT05147 .
M6ACROT05148 .
M6ACROT05149 .
M6ACROT05150 .
M6ACROT05151 .
M6ACROT05152 "Female C57BL/6 mice, aged eight weeks, were selected for the study. The mice were divided into three groups with six mice per group. After intramuscular anesthesia (xylazine 10 mg/kg, ketamine 100 mg/kg), two groups underwent bilateral ovariectomy (OVX), while the remaining group had a sham operation. Ovariectomized mice were administered weekly intratibial injections of Mettl14 overexpression adenovirus containing at a concentration of 109 plaque-forming unit per injection, for a duration of 8 weeks. The mice were humanely euthanized one week after the final injection, and their tibia were gathered for further analysis. This study was approved by the ethics committee of Tongji University (NO: TJBH00823101). All the experimental methods were carried out in accordance with the approved guidelines. All experimental procedures involving mice were carried out in strict accordance with the recommendations in the ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments)."
M6ACROT05153 "After anesthesia with ketamine-xylazine(intraperitoneal injection, 60 and 5 mg/kg, respectively), the rats were operated to remove the bilateral ovaries. For the rats in the Sham group, only the fat around the ovaries was surgically removed. Four weeks after the operation, the animals were orally treated with ICA (250 mg/kg) daily for 10 weeks ."
M6ACROT05154 "Male C57BL/6 mice (25-30 g) were obtained from Vital River Laboratory Animal Technology (Beijing, China) and were provided adaptive feeding for a week at the suitable temperature and humidity. All animals were housed in micro-isolator cages with free access to food and water according to the Guide for the Care and Use of Laboratory Animals. The myocardial I/R operation were followed by previous research (Song et al., 2015). The mice were randomly divided into myocardial I/R group (n = 10) and sham group (n = 10). Mice were anesthetized (50 mg/kg pentobarbital sodium, intraperitoneal injection) before assays. The supine of mice were fixed on the operating table connected with the standard lead II electrocardiogram. The left thorax was cut to expose the heart and the left anterior descending (LAD) coronary artery was ligated by 7/0 sterile suture. Myocardial ischemia was induced by LAD ligation for 30 min followed by 120 min of reperfusion. Sham group mice underwent the same surgical procedures without LAD coronary artery ligation. After assay, the surviving animals were transferred to institution's animal department for euthanizing mice."
M6ACROT05155 "For subcutaneous tumor models, 1.5 × 106 HUH7 cells (e.g., negative control, AC115619-overexpressing cells) in 50 μ L PBS with 50 μ L Matrigel were administered to male BALB/c nude mice (4-6 weeks of age) by subcutaneous injection and cell line-derived xenografts (CDX) were established.For lung metastasis models, luciferase-tagged lentivirus transfected into HUH7 cells (1 × 106/100 μ L PBS) was injected into male BALB/c nude mice (4-6 weeks of age) via tail vein."
M6ACROT05156 "For subcutaneous tumor models, 1.5 × 106 HUH7 cells (e.g., negative control, AC115619-overexpressing cells) in 50 μ L PBS with 50 μ L Matrigel were administered to male BALB/c nude mice (4-6 weeks of age) by subcutaneous injection and cell line-derived xenografts (CDX) were established.For lung metastasis models, luciferase-tagged lentivirus transfected into HUH7 cells (1 × 106/100 μ L PBS) was injected into male BALB/c nude mice (4-6 weeks of age) via tail vein."
M6ACROT05157 .
M6ACROT05158 BEL-7404 cells or BEL-7404 miR4458HG-KO cells were infused in the right flank of randomly selected 4-week-old male BALB/c mice.
M6ACROT05159 BEL-7404 cells or BEL-7404 miR4458HG-KO cells were infused in the right flank of randomly selected 4-week-old male BALB/c mice.
M6ACROT05160 "To investigate chronic Cd-induced mitochondrial dysfunction and neurotoxicity in vivo, C57BL/6J mice were randomly assigned to 2 groups: (i) control group mice (n = 20) and (ii) Cd exposure group mice (n = 20)."
M6ACROT05161 .
M6ACROT05162 .
M6ACROT05163 "Each nude mouse from the TE-1 group was injected with 0.2 mL TE-1 cell suspensions (1 × 106 cells), and each mouse from the sh-LINC00858 group or the sh-NC group was injected respectively with an equal volume of TE-1 cells stably transfected with sh-LINC00858 or sh-NC."
M6ACROT05164 .
M6ACROT05165 "One microliter of AAV [pAAV-hSyn-HA-hM3D(Gq)-IRES-mCitrine, AAV9-hSyn-EGFP-miR-NC-knockdown (KD) or AAV9-hSyn-EGFP-miR-200c-3p-KD; 5 × 1012 v.g/mL] was injected into the mouse motor cortex (M1; 1 mm anterior, 1.5 mm lateral to the bregma and at a depth of 1 mm)."
M6ACROT05166 .
M6ACROT05167 .
M6ACROT05168 .
M6ACROT05169 A concentration of 1 × 107 CAL62 cells stably transfected with OE METTL3 or OE METTL3/shPAX8-transfected CAL-62 cells were injected subcutaneously into the dorsal flanks of the mice (five mice per group) to establish a xenograft model.
M6ACROT05170 A concentration of 1 × 107 CAL62 cells stably transfected with OE METTL3 or OE METTL3/shPAX8-transfected CAL-62 cells were injected subcutaneously into the dorsal flanks of the mice (five mice per group) to establish a xenograft model.
M6ACROT05171 .
M6ACROT05172 .
M6ACROT05173 .
M6ACROT05174 "BALB/c nude mice were purchased from the Slac Laboratories (Shanghai, China) and randomly divided into two groups. The xenograft mouse model was generated via tail-vein injection of METTL14-expressing T24 cells (5 × 105 cells per mouse) into experimental mice while those injected with pcDNA3.1 empty vector transfected cells as control."
M6ACROT05175 "BALB/c nude mice were purchased from the Slac Laboratories (Shanghai, China) and randomly divided into two groups. The xenograft mouse model was generated via tail-vein injection of METTL14-expressing T24 cells (5 × 105 cells per mouse) into experimental mice while those injected with pcDNA3.1 empty vector transfected cells as control."
M6ACROT05176 "The mice were subcutaneously inoculated with TU177 cells stably transfected with lentiviruses carrying sh-circCDK1/sh-NC, respectively (2.5 × 106, 200ul)."
M6ACROT05177 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
M6ACROT05178 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
M6ACROT05179 "To determine whether lnc-H2AFV-1 overexpression could enhance tumorigenicity in vivo, 1 × 106 HN6 cells stably transduced with LV-lnc-H2AFV-1 or LV-NC were subcutaneously injected into the right and left flanks of six mice."
M6ACROT05180 "Pregnant C57/BL6 were randomly assigned into four groups (each n=10): (1) corn oil group, (2) BaP group, (3) BaP +AS-NC group, (4) BaP +AS-hz06 group."
M6ACROT05181 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
M6ACROT05182 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
M6ACROT05183 Liver metastasis model construction: 2 × 106 CRC cells were injected into the tail vein of nude mice.
M6ACROT05184 "For the UUO model, the left ureter was exposed via a midline incision and ligated twice 15 mm below the renal pelvis with 4-0 silk after anesthetization. The sham group was similarly handled without ureteral ligation. For the renal IRI model, bilateral renal arteries were clamped for 30 min."
M6ACROT05185 "For the UUO model, the left ureter was exposed via a midline incision and ligated twice 15 mm below the renal pelvis with 4-0 silk after anesthetization. The sham group was similarly handled without ureteral ligation. For the renal IRI model, bilateral renal arteries were clamped for 30 min."
M6ACROT05186 "For the UUO model, the left ureter was exposed via a midline incision and ligated twice 15 mm below the renal pelvis with 4-0 silk after anesthetization. The sham group was similarly handled without ureteral ligation. For the renal IRI model, bilateral renal arteries were clamped for 30 min."
M6ACROT05187 "For the UUO model, the left ureter was exposed via a midline incision and ligated twice 15 mm below the renal pelvis with 4-0 silk after anesthetization. The sham group was similarly handled without ureteral ligation. For the renal IRI model, bilateral renal arteries were clamped for 30 min."
M6ACROT05188 .
M6ACROT05189 .
M6ACROT05190 .
M6ACROT05191 "40 C57BL/6 J male mice (8 weeks old, 20-22 g) obtained from SLAC Experimental Animal Co., Ltd. were randomly assigned into five groups (n = 8): sham, I/R, I/R + Exos, I/R + inhibitor NC-Exos, and I/R + miR-381 inhibitor-Exos."
M6ACROT05192 "For the xenograft model, 100μ L cell suspensions containing 1× 106 CRC cells sin 100 μ LPBS were injected into the armpits of the mouse limbs which divided into six groups (shNC, shHMGCS2, shLOC+OE-HMGCS2, shLOC, shHMGCS2+OE-IGF2BP1, shLOC+OE-GF2BP1)."
M6ACROT05193 "To examine the effects of piRNA-14633 on subcutaneous xenograft growth, BALB/c nude mice (Beijing Vital River Laboratory Animal Technology) were subcutaneously injected with 0.1 mL of cell suspension containing 2 × 106 cells. Tumor volume (mm3) was measured every 4 days using a Vernier caliper and calculated as 0.4 x (short length)2 × long length."
M6ACROT05194 .
M6ACROT05195 .
M6ACROT05196 .
M6ACROT05197 "MDA-MB-231 cells (1× 106 cells/mouse) were mixed with an equivalent number of NFs/Ctrl, CAFs/shNC, CAFs/sh lncSNHG5, CAFs/shZNF281, CAFs/sh lncSNHG5/ZNF281, CAFs/RS102895, CAFs/Maraviroc or CAFs/Cenicriviroc in 200 μ l PBS:Matrigel at a ratio of 1:1."
M6ACROT05198 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μ L of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
M6ACROT05199 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μ L of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
M6ACROT05200 "To investigate tumor metastasis, approximately 1 × 106 HCT116 (Control or circEZH2 KD) cells suspended in 100 μ L of 1 × PBS per mouse were injected into the tail vein of male BALB/c nude mice."
M6ACROT05201 "Logarithmic phase PDAC cells (4 × 106/100 μ l) were infected using lentiviruses possessing constructs. Then, the nude mice (n = 5 per group) were inoculated into the dorsal flank subcutaneously."
M6ACROT05202 "The KYSE-150 cells were subcutaneously inoculated on the right side at a dosage of 106 cells per mouse. After the tumor grew to approximately 50 mm3, mice were randomly divided into four groups (n = 6): Control, CisR-exo, Cis, and Cis + CisR-exo. Then, normal saline or cisplatin (20 mg/kg; twice a week) was intratumorally injected alone or combined with CisR-exos (10 μ g; once every two days)."
M6ACROT05203 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
M6ACROT05204 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
M6ACROT05205 "5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice."
M6ACROT05206 .
M6ACROT05207 .
M6ACROT05208 "BGC-823 (3 × 106) cells that stably expressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected into the left side of each mouse. For cell metastasis experiments in vivo, BGC-823 (3 × 106) cells that stably overexpressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected through tail vein."
M6ACROT05209 "BGC-823 (3 × 106) cells that stably expressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected into the left side of each mouse. For cell metastasis experiments in vivo, BGC-823 (3 × 106) cells that stably overexpressed or silenced ALKBH5 and LINC00659 and their paired control cells were injected through tail vein."
M6ACROT05210 "Mice were blindly grouped into sh-NC, sh-LRRC75A-AS1, OE-NC, and OE-LRRC75A-AS1 groups (n = 7 per group). sh-NC or sh-LRRC75A-AS1-transfected HeLa cells and OE-NC or OE-LRRC75A-AS1-transfected CaSki cells were subcutaneously injected into the right flank (1 × 106 cells in 200 μ L PBS/mouse)."
M6ACROT05211 "Six to eight weeks old male BALB/c nu/nu mice were used. For orthotopic implantation, 1 × 107 PBS suspended cells was injected subcutaneously. For the spleen injection model, 5 × 105 cells were injected into the spleen through an incision on the left side of abdomen."
M6ACROT05212 "When the xenografted tumors grew up to 50 mm3, 18 mice were randomly divided into three groups (n = 6 mice /group): NC group (mice have systematically injected NC lentivirus through the lateral tail vein), sh-OIP5-AS1-1 group (mice have systematically injected sh-OIP5-AS1-1 lentivirus through the lateral tail vein), sh-OIP5-AS1-2 group (mice have systematically injected sh-OIP5-AS1-2 lentivirus through the lateral tail vein).A total of 18 6-week-old healthy male NOD/SCID mice (weighing 20 ± 2 g) were randomly assigned to one of the three groups (n = 6 mice /group) and injected through the tail vein with 1 × 106 NC, sh-OIP5-AS1-1, or sh-OIP5-AS1-2 stable AGS cells or Ctrl or OIP5-AS1 stable MGC823 cells co-transfected with lentiviruses carrying the luciferase reporter gene. "
M6ACROT05213 .
M6ACROT05214 .
M6ACROT05215 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT05216 The cell mass (5 × 106) was dissolved in 200 μ l PBS and subcutaneously injected into the left side of each mouse.
M6ACROT05217 The cell mass (5 × 106) was dissolved in 200 μ l PBS and subcutaneously injected into the left side of each mouse.
M6ACROT05218 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group)."
M6ACROT05219 "Roughly 1 × 107 of MCF-7 or MDA-MB-231 cells stably transfected with ADAR1 sgRNA or Ctrl sgRNA (as described above) were injected subcutaneously into the thighs of the female athymic nude mice (5 weeks old, 17-18 g, three mice per group)."
M6ACROT05220 .
M6ACROT05221 .
M6ACROT05222 "Briefly, 5 × 105 LoVo cells stably transfected with sh-NC or sh-circASPH#1 were subcutaneously injected into the right back of each mice in 2 groups. To evaluate the effect of STING-overexpressed macrophages on tumor growth, LoVo cells stably transfected with sh-NC or sh-circASPH#1 were separately cocultured with STING-overexpressed macrophages."
M6ACROT05223 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT05224 "To establish xenograft models, five-week-old male mice were orthotopically injected with the same number of the PC9/GR cells. Tumours had developed after 4 days, at which time the xenografted mice were randomly divided into the following two experimental groups (each group with 6 mice): (1) the control group and (2) the gefitinib group."
M6ACROT05225 A total of 1 × 106 luciferase-labeled tumor cells were injected subcutaneously with SN-exo or Ctr-exo.
M6ACROT05226 .
M6ACROT05227 "For subcutaneous HCC mice xenograft model, the cell suspension was injected into the right flank of the mice (n = 5 per group)."
M6ACROT05228 "For subcutaneous HCC mice xenograft model, the cell suspension was injected into the right flank of the mice (n = 5 per group)."
M6ACROT05229 Xenograft mouse models were established by subcutaneously inoculating 1 × 107 transfected ACHN cells into mouse left flank.
M6ACROT05230 "To establish a murine subcutaneous tumor model, sh-PLAGL2 or sh-PLAGL2+oe-Snail HGC-27 stable transfected HGC-27 cells (5× 106 cells/kg) subcutaneously to the right of the dorsal midline of mice (n=6 for each)."
M6ACROT05231 .
M6ACROT05232 .
M6ACROT05233 .
M6ACROT05234 .
M6ACROT05235 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
M6ACROT05236 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
M6ACROT05237 .
M6ACROT05238 .
M6ACROT05239 .
M6ACROT05240 "Mice were randomized into three groups (n = 7/group), 1 × 107 PC9 cells absorbed exosomes were subcutaneously injected into the Bilateral groin of mice. Treatment began 1 week following injection, the mice were intraperitoneally injected with gefitinib (30 mg/kg/day)."
M6ACROT05241 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
M6ACROT05242 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
M6ACROT05243 "Animal experiments were approved by the Hainan Affiliated Hospital of Hainan Medical University. Male BALB/c mice (Vital River, Beijing, China) were subcutaneously injected with H322 cells (1 × 106) transfected with ov-NC, ov-FTO, ov-GAS5 or ov-FTO + ov-GAS5 (n = 5/group). Tumor volume was measured every 7 days, and mice were sacrificed and the tumor tissues were removed for weighting and analyzing after 35 days. Also, tumor tissues were prepared into paraffin sections to carry out Ki67 immunohistochemistry (IHC) staining using SP Kit (Solarbio, Beijing, China) and TUNEL staining using Colorimetric TUNEL Apoptosis Assay Kit (Beyotime) basing on kit instructions."
M6ACROT05244 "The Laboratory Animal Center of Soochow University provided female BALB/c nude mice (5 weeks old). Mice were kept under specific pathogen-free conditions. They were injected intravenously (i.v.) with FTO-overexpressing and control A549 cells (1.8 × 106 cells/mouse) to establish the in vivo model of NSCLC metastasis, and were then gavaged with dimethyl sulfoxide (DMSO) (25 mg/kg, daily) or the FAK inhibitor defactinib (VS6063) (Cat#S7654, Selleck, USA) (25 mg/kg, daily) beginning in the fifth week after injection. The mice were euthanized eight weeks after being inoculated, and their lungs were taken out and preserved in Bouin's solution for macroscopic investigation of metastatic nodules. Hematoxylin and eosin (H&E) staining of lung tissues was used to look for micrometastatic foci."
M6ACROT05245 "Five-week-old female BALB/c nude mice were purchased from the Cancer Institute of the Chinese Academy of Medical Science (Beijing, China). Mice were randomly divided into two groups (eight mice per group), and 3× 106 H1299 or H1299-LV-FTO-KD cells were injected subcutaneously into the flank of each mouse. For each group, four mice were treated with PEITC (100 mg/kg) intraperitoneally every two days for 14 days beginning the fifth week after cell injection, while the other four mice were treated with PBS as a control. At the end of the experiment, mice were euthanized, and tumor tissues were dissected for further analysis. Tumor weights were measured, and volumes were calculated (V = D/2 × d2, V: volume; D: longitudinal diameter; d: latitudinal diameter)."
M6ACROT05246 .
M6ACROT05247 .
M6ACROT05248 .
M6ACROT05249 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
M6ACROT05250 "Equal numbers of A549 cells expressing either control or shFTO were injected subcutaneously, within 30 min of harvesting, over the right and left flanks in male nu/nu mice between 4 and 6 weeks of age."
M6ACROT05251 .
M6ACROT05252 .
M6ACROT05253 .
M6ACROT05254 "Mice were randomized into three groups (n = 7/group), 1 × 107 PC9 cells absorbed exosomes were subcutaneously injected into the Bilateral groin of mice. Treatment began 1 week following injection, the mice were intraperitoneally injected with gefitinib (30 mg/kg/day)."
M6ACROT05255 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
M6ACROT05256 "The nude mice were maintained under pathogen-free conditions and kept under timed lighting conditions mandated by the committee with food and water provided ad libitum. For xenograft experiments, nude mice were injected subcutaneously with 5 × 106 cells resuspended in 0.1 mL PBS. When a tumor was palpable, it was measured every 3 days."
M6ACROT05257 "Animal experiments were approved by the Hainan Affiliated Hospital of Hainan Medical University. Male BALB/c mice (Vital River, Beijing, China) were subcutaneously injected with H322 cells (1 × 106) transfected with ov-NC, ov-FTO, ov-GAS5 or ov-FTO + ov-GAS5 (n = 5/group). Tumor volume was measured every 7 days, and mice were sacrificed and the tumor tissues were removed for weighting and analyzing after 35 days. Also, tumor tissues were prepared into paraffin sections to carry out Ki67 immunohistochemistry (IHC) staining using SP Kit (Solarbio, Beijing, China) and TUNEL staining using Colorimetric TUNEL Apoptosis Assay Kit (Beyotime) basing on kit instructions."
M6ACROT05258 "The Laboratory Animal Center of Soochow University provided female BALB/c nude mice (5 weeks old). Mice were kept under specific pathogen-free conditions. They were injected intravenously (i.v.) with FTO-overexpressing and control A549 cells (1.8 × 106 cells/mouse) to establish the in vivo model of NSCLC metastasis, and were then gavaged with dimethyl sulfoxide (DMSO) (25 mg/kg, daily) or the FAK inhibitor defactinib (VS6063) (Cat#S7654, Selleck, USA) (25 mg/kg, daily) beginning in the fifth week after injection. The mice were euthanized eight weeks after being inoculated, and their lungs were taken out and preserved in Bouin's solution for macroscopic investigation of metastatic nodules. Hematoxylin and eosin (H&E) staining of lung tissues was used to look for micrometastatic foci."
M6ACROT05259 "Five-week-old female BALB/c nude mice were purchased from the Cancer Institute of the Chinese Academy of Medical Science (Beijing, China). Mice were randomly divided into two groups (eight mice per group), and 3× 106 H1299 or H1299-LV-FTO-KD cells were injected subcutaneously into the flank of each mouse. For each group, four mice were treated with PEITC (100 mg/kg) intraperitoneally every two days for 14 days beginning the fifth week after cell injection, while the other four mice were treated with PBS as a control. At the end of the experiment, mice were euthanized, and tumor tissues were dissected for further analysis. Tumor weights were measured, and volumes were calculated (V = D/2 × d2, V: volume; D: longitudinal diameter; d: latitudinal diameter)."
M6ACROT05260 .
M6ACROT05261 .
M6ACROT05262 .
M6ACROT05263 .
M6ACROT05264 .
M6ACROT05265 "2 × 106 stably transfected 22Rv-1 cells were injected via the tail vein into BALB/c nude mice. After 8 weeks, tumor metastasis loci were measured via in vivo imaging system (IVIS). After 8 weeks, the mice were euthanized, the lungs were surgically dissected and embedded in paraffin. Then, the specimens were stained with hematoxylin and eosin."
M6ACROT05266 "The mice were randomly assigned to two groups, with five in each group. Vector control or IGF2BP3 knockdown BFTC905 cells (1 × 106 cells in 100 μl PBS) were subcutaneously injected into the right flanks of each mouse. "
M6ACROT05267 "1 × 105 parental HT29-shNC cells, HT29-shYTHDF1 cells, HT29-shNC colonospheres, and HT29-shYTHDF1 colonospheres were inoculated subcutaneously into the left inguinal folds of the nude mice."
M6ACROT05268 .
M6ACROT05269 .
M6ACROT05270 "Sh-GID8 stable cells (2 × 106) were inoculated subcutaneously into the left axilla of athymic nude mice (n = 5 mice/group). Every three days, the tumor's size was measured with a digital caliper, and its volume was computed using the following formula: tumor volume (mm3) = length × width × width × 0.52. When the subcutaneous tumor reached approximately 50-100 mm3, glutamine (75 mg/kg) [13] or PBS was injected intratumorally every day. Tumors were collected for TUNEL staining or other analysis when tumor volumes reached the humane endpoint. For the metastasis model, the constructed HCT116 cells (2 × 106) with stable YTHDF1 or GID8 knockdown were injected into nude mice through the tail vein (n = 4 or n = 5 mice/group). Two months later, all the mice were sacrificed to observe tumor metastasis in the lungs."
M6ACROT05271 "All nude mice were subcutaneously injected with A549 cells (2 × 106). When tumor volume reached about 100 mm3, the nude mice were randomly divided into three groups (n = 6). From now on (day 0), DDP (20 mg/kg) was intraperitoneally injected into the mice twice a week, and exosome (10 μg) derived from A549/DDP cells transfected with sh-NC or sh-circVMP1 was intratumorally injected into the mice once every two days."
M6ACROT05272 "All nude mice were subcutaneously injected with A549 cells (2 × 106). When tumor volume reached about 100 mm3, the nude mice were randomly divided into three groups (n = 6). From now on (day 0), DDP (20 mg/kg) was intraperitoneally injected into the mice twice a week, and exosome (10 μg) derived from A549/DDP cells transfected with sh-NC or sh-circVMP1 was intratumorally injected into the mice once every two days."
M6ACROT05273 200 μL of HepG2 cells (5 × 106 cells) harboring sh-NC or sh-LINC00942 was subcutaneously injected into right flank of mice.
M6ACROT05274 .
M6ACROT05275 .
M6ACROT05276 .
M6ACROT05277 .
M6ACROT05278 "nude mice were randomly divided into four groups and injected subcutaneously with SW480 (Vector), SW480 (miR-6125), RKO (Vector) or RKO (miR-6125) cells on the back region (5.0 × 106 cells in 100 μl PBS/mouse)."
M6ACROT05279 .
M6ACROT05280 .
M6ACROT05281 .
M6ACROT05282 "Female C57BL/6 mice were fed under pathogen-free conditions and kept in a controlled environment (temperature 23 ± 1°C; humidity 50-60%), and subjected to a 12-hour light/dark cycle artificial simulation. Eight-week-old mice were selected for surgery and randomly divided into sham group (n = 6) and ovariectomized model group (OVX, n = 24)."
M6ACROT05283 "For the tumorigenicity studies, 3 × 106 HCC827 cells stably expressing sh-LINC01833 or sh-NC were injected subcutaneously into the ventral side of male BALB/c nude mice in the according groups with five mice in each group. Five mice were sampled each time."
M6ACROT05284 "For xenograft tumor model, 5-week-old male BALB/c nude mice (n = 10) were gained from Beijing Laboratory Animal Center (Beijing, China). BC cells (MCF-7/ADR, 1 × 107 cells/0.2 mL PBS:Matrigel = 1:3, v/v) were transfected with NC or sh-DLGAP1-AS1 and then subcutaneously implanted into the flank of mice."
M6ACROT05285 "For xenograft tumor model, 5-week-old male BALB/c nude mice (n = 10) were gained from Beijing Laboratory Animal Center (Beijing, China). BC cells (MCF-7/ADR, 1 × 107 cells/0.2 mL PBS:Matrigel = 1:3, v/v) were transfected with NC or sh-DLGAP1-AS1 and then subcutaneously implanted into the flank of mice."
M6ACROT05286 "For xenograft tumor model, 5-week-old male BALB/c nude mice (n = 10) were gained from Beijing Laboratory Animal Center (Beijing, China). BC cells (MCF-7/ADR, 1 × 107 cells/0.2 mL PBS:Matrigel = 1:3, v/v) were transfected with NC or sh-DLGAP1-AS1 and then subcutaneously implanted into the flank of mice."
M6ACROT05287 .
M6ACROT05288 .
M6ACROT05289 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
M6ACROT05290 .
M6ACROT05291 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
M6ACROT05292 .
M6ACROT05293 "A subcutaneous GC xenograft model of MGC-803 cells was established in nude mice. The mice were then randomly divided into 3 equal groups of 6: sh-NC + oe-NC, sh-EED + oe-NC, and sh-EED + oe-CDCP1. Tumor growth was observed and recorded by measuring tumor long diameter (A) and short diameter (B) using a vernier caliper after inoculation. "
M6ACROT05294 "For the animal model of BM, eight BALB/c-nu mice (male, 4-6 weeks old) in the indicated groups were injected with PC-3 cells (1 × 106) in 100 μl PBS into the left cardiac ventricle and further analyzed and measured by In Vivo Imaging System (IVIS, Caliper Life Sciences), X-ray, hematoxylin and eosin (H&E), and IHC staining."
M6ACROT05295 "To evaluate the potential roles of RP11 in the growth of CRC, ten female BALB/c nude mice randomly divided into two groups. HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 μl normal medium + 100 μl Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth. "
M6ACROT05296 "To evaluate the potential roles of RP11 in the growth of CRC, ten female BALB/c nude mice randomly divided into two groups. HCT-15 RP11 stable overexpression or control cells (2 × 106 per mouse) diluted in 100 μl normal medium + 100 μl Matrigel (BD Biosciences) were subcutaneously injected into immunodeficient mice to investigate tumour growth. "
M6ACROT05297 "PCa cells were resuspended in serum-free RPMI-1640 medium (Gibco, USA) to cell suspension at a density of 1 × 106 cells/200 μL. The Balb/c nude mice were randomly divided into three groups (n = 12 in each group), and the tumorigenesis experiment lasted for 4 weeks. "
M6ACROT05298 "Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rbeta-null (NSG) mice. For tail vein injection, stable DMDRMR KD and control cell lines (5 × 106 cells/0.1 mL PBS) were injected into the lateral tail vein of age-matched NSG mice."
M6ACROT05299 "Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rbeta-null (NSG) mice. For tail vein injection, stable DMDRMR KD and control cell lines (5 × 106 cells/0.1 mL PBS) were injected into the lateral tail vein of age-matched NSG mice."
M6ACROT05300 "Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rbeta-null (NSG) mice. For tail vein injection, stable DMDRMR KD and control cell lines (5 × 106 cells/0.1 mL PBS) were injected into the lateral tail vein of age-matched NSG mice."
M6ACROT05301 A549 cells (106 cells per mouse) transfected with miR-4443 mimic or mimic-NC were injected subcutaneously to generate subcutaneous tumors.
M6ACROT05302 Female BALB/c nude mouse (4 weeks) was used to establish a xenograft transplant model. DLD-1 cells stably transfected with sh-linc00659 or scramble were subcutaneously inoculated in the mouse with 2 × 106 cells.
M6ACROT05303 .
M6ACROT05304 .
M6ACROT05305 Approximately 5 × 106 control and ILF3-AS1 silencing Huh7 cells were subcutaneously implanted into the right flank of nude mice. Xenograft size was measured every 7 days.
M6ACROT05306 Approximately 5 × 106 control and ILF3-AS1 silencing Huh7 cells were subcutaneously implanted into the right flank of nude mice. Xenograft size was measured every 7 days.
M6ACROT05307 .
M6ACROT05308 5 × 106 cells were suspended in 100 uL PBS and then were inoculated subcutaneously.
M6ACROT05309 Xenograft mouse tumor model was established by inoculating MKN-45 cells transfected with LV Sh355 lentivirus or control LV ShNC lentivirus (2 × 106 in 200 μl sterile 1×phosphate-buffered saline [PBS]) subcutaneously into the right armpit of 5-6-week-old male BALB/c nude mice. GC pulmonary metastatic model was established by injecting MKN-45 cells transfected with LV Sh355 lentivirus or control LV ShNC lentivirus (2 × 106 in 200 μl sterile 1 × PBS) into the caudal vein of 5-6-week-old male BALB/c nude mice.
M6ACROT05310 .
M6ACROT05311 .
M6ACROT05312 .
M6ACROT05313 .
M6ACROT05314 Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained. MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice.
M6ACROT05315 Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained. MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice.
M6ACROT05316 Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained. MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice.
M6ACROT05317 Male BALB/c nude mice (aged 4-6 weeks; n = 5/group) were obtained. MHCC97H or Huh7 cells (2 × 106 cells/mouse) stably transfected with lentivirus containing different plasmids in 100 μL DMEM were subcutaneously or orthotopically implanted into the nude mice.
M6ACROT05318 "5 × 106 stable LncRNA-PACERR knockdown (KD) and control THP-1 cell lines treated with PMA and 1 × 107 PATU-8988/0037 wild-type cells were co-injected subcutaneously into per BALB/c nude mice. For liver metastases model, 5 × 106 stable LncRNA-PACERR knockdown and control THP-1 cells treated with PMA for two days and 1 × 107 PATU-8988/0037 wild-type cells were co-injected into spleens of per BALB/c nude mice."
M6ACROT05319 "5 × 106 stable LncRNA-PACERR knockdown (KD) and control THP-1 cell lines treated with PMA and 1 × 107 PATU-8988/0037 wild-type cells were co-injected subcutaneously into per BALB/c nude mice. For liver metastases model, 5 × 106 stable LncRNA-PACERR knockdown and control THP-1 cells treated with PMA for two days and 1 × 107 PATU-8988/0037 wild-type cells were co-injected into spleens of per BALB/c nude mice."
M6ACROT05320 "CRC cells SW480 at logarithmic growth phase were prepared into cell suspension with a concentration of about 1 × 107/100 L, which was then injected into the left axilla of nude mice with a 1 ml syringe to establish a subcutaneous mouse xenograft model. "
M6ACROT05321 .
M6ACROT05322 "To investigate the effect of YTHDF1 on breast cancer progression in mice, the reared mice were randomly divided into two groups (n = 5). Each mouse was injected subcutaneously with 5 × 106 units of tumor cells to construct the tumor model. In the shCtrl group, shCtrl-transfected 4T1 (ATCC, #CRL-2539) cells were injected subcutaneously into mice to construct YTHDF1-normal 4T1 tumors. To investigate the effect of miR-16-5p on breast cancer progression by regulating the expression of YTHDF1, three experimental groups (n = 5) were designed for the in vivo experiment, which were the agomiR-nc+antgomiR-nc, agomiR-16-5p + antgomiR-nc, and agomiR-16-5p + antgomiR-16-5p groups"
M6ACROT05323 "For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. "
M6ACROT05324 "For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. "
M6ACROT05325 "For tumorigenesis analysis, AGS cells (1 × 106) with stable knockdown of AGAP2-AS1 or scramble, were injected into mice. "
M6ACROT05326 "Ten BALB/c nude mice (21 days) were randomly divided into two groups (NC group and si-CACNA1G-AS1 group). In the NC group, we performed subcutaneous injection with 0.2 ml SKOV3 cells (2 × 107/ml) on the back of each mouse, while in the si-CACNA1G-AS1 group, we chose 0.2 ml CACNA1G-AS1 knockout SKOV3 cells (2 × 107/ml) for injection."
M6ACROT05327 .
M6ACROT05328 Four-week-old female nonobese diabetic Prkdcscid Il2rgtm1/Bcgen (NSG) mice weighing 10 to 15 g were purchased from Biocytogen Company and fed in the specific pathogen-free barrier system. Mice were subcutaneously injected in the left flank with 106 SU-DH-L8 cells in 50% Matrigel (Corning).
M6ACROT05329 .
M6ACROT05330 .
M6ACROT05331 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05332 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05333 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05334 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05335 .
M6ACROT05336 "The mice were randomly divided into four groups (n=5 per group), labeled as OE-vector (caudal vein injection of 2×106 AN3CA cells), OE-SLERT (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells), OE-SLERT+ASO-BDNF (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells transfected with ASO-BDNF), and OE-SLERT+k252a (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells and intraperitoneal injection of 25μg/kg k252a)."
M6ACROT05337 "The mice were randomly divided into four groups (n=5 per group), labeled as OE-vector (caudal vein injection of 2×106 AN3CA cells), OE-SLERT (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells), OE-SLERT+ASO-BDNF (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells transfected with ASO-BDNF), and OE-SLERT+k252a (caudal vein injection of 2×106 SLERT-overexpressing AN3CA cells and intraperitoneal injection of 25μg/kg k252a)."
M6ACROT05338 "GBM cells were infected with lentivirus overexpressing FTO (Lv-FTO) or control lentivirus (Lv-NC), followed by subcutaneous injection with 2.5×105 cells in a total volume of 200 μl into the right dorsal-lateral flanks of mice. "
M6ACROT05339 .
M6ACROT05340 .
M6ACROT05341 .
M6ACROT05342 .
M6ACROT05343 "For the proliferation assays, C4-2B cells of KIF3C knockdown, negative control (1×106/200μl) were subcutaneously injected into BALB/c nude mice. The tumors were dissected and weighed (4-6 weeks old, male)."
M6ACROT05344 "For the proliferation assays, C4-2B cells of KIF3C knockdown, negative control (1×106/200μl) were subcutaneously injected into BALB/c nude mice. The tumors were dissected and weighed (4-6 weeks old, male)."
M6ACROT05345 .
M6ACROT05346 "Five- to 6-week-old male athymic nude mice purchased by Charles River were used for the xenograft model. 769-P cells stably expressing Ctrl, FTO and FTO-mut were trypsinized and washed twice to thrice with standardized PBS, and then, 5 × 106 cells in 100 uL of PBS was subcutaneously injected into the flanks of the mice (five mice per group). Mice were monitored twice every week for tumour growth, and tumour diameters were measured using a caliper."
M6ACROT05347 "For in vivo tumour growth studies, 143B cells were subcutaneously injected into the dorsal flanks of 5-week-old male BALB/c nude mice (n = 5 per group) in a blind, randomized fashion. The growth and weight of xenografts were detected 1 month later. In experimental metastasis studies, tail vein injection of 143B cells was performed in a blind, randomized fashion in 5-week-old male BALB/c nude mice (n = 5 per group). Metastasis counts and survival time of each mouse were monitored and recorded, and the xenografts were studied by haematoxylin and eosin (H&E) staining. "
M6ACROT05348 "For in vivo tumour growth studies, 143B cells were subcutaneously injected into the dorsal flanks of 5-week-old male BALB/c nude mice (n = 5 per group) in a blind, randomized fashion. The growth and weight of xenografts were detected 1 month later. In experimental metastasis studies, tail vein injection of 143B cells was performed in a blind, randomized fashion in 5-week-old male BALB/c nude mice (n = 5 per group). Metastasis counts and survival time of each mouse were monitored and recorded, and the xenografts were studied by haematoxylin and eosin (H&E) staining. "
M6ACROT05349 "For in vivo tumour growth studies, 143B cells were subcutaneously injected into the dorsal flanks of 5-week-old male BALB/c nude mice (n = 5 per group) in a blind, randomized fashion. The growth and weight of xenografts were detected 1 month later. In experimental metastasis studies, tail vein injection of 143B cells was performed in a blind, randomized fashion in 5-week-old male BALB/c nude mice (n = 5 per group). Metastasis counts and survival time of each mouse were monitored and recorded, and the xenografts were studied by haematoxylin and eosin (H&E) staining. "
M6ACROT05350 .
M6ACROT05351 .
M6ACROT05352 .
M6ACROT05353 .
M6ACROT05354 "Huh7 cells with or without sorafenib resistance were subcutaneously injected into nude mice. The nude mice then grew naturally for 4 weeks, and the subcutaneous tumor volume was measured every week. After the experiment, tumor tissues were dissected, weighed, and photographed, followed by qRT-PCR and Western blot assays testing gene and protein expression, respectively."
M6ACROT05355 "Huh7 cells with or without sorafenib resistance were subcutaneously injected into nude mice. The nude mice then grew naturally for 4 weeks, and the subcutaneous tumor volume was measured every week. After the experiment, tumor tissues were dissected, weighed, and photographed, followed by qRT-PCR and Western blot assays testing gene and protein expression, respectively."
M6ACROT05356 .
M6ACROT05357 .
M6ACROT05358 .
M6ACROT05359 .
M6ACROT05360 "Mice were subcutaneously injected with 2.5 × 106 U251 cells stably infected with OE-NC, OE-JMJD1C, OE-NC and sh-NC, OE-JMJD1C and sh-NC or OE-JMJD1C and sh-SOCS2 (n = 10 in each group) in 0.1 ml PBS. Tumors were visible one week after injection, and the tumor size was subsequently assessed with Vernier calipers at one-day intervals."
M6ACROT05361 "BALB/c nu/nu mice were subcutaneously injected with 8 × 106 tumor cells (control, VIM-AS1-overexpressing or VIM-AS1- and EPHA3-overexpressing cells). "
M6ACROT05362 .
M6ACROT05363 Athymic BALB/c mice were injected with LCSC cells at the armpit area subcutaneously.
M6ACROT05364 .
M6ACROT05365 "2 × 106 of TBUR1-overexpressing or control A549 cells were injected into nude mice through tail vein (n = 8/group). After 8 weeks, the mice were sacrificed, and the lungs were removed and sliced after fixation. H&E staining was then performed."
M6ACROT05366 .
M6ACROT05367 Suspension of H1299 cells (5.0 × 105) was subcutaneously injected into the right flanks of the mice.
M6ACROT05368 "For the therapeutic intervention, AAV5/sh-circFTO or AAV5/sh-NC vectors were administered on day 21, concurrently with booster vaccination. Ankle joint cavities received an injection of 5 L of AAV5 solution containing 2 × 109 viral particles per L. This injection was repeated weekly for a duration of 5 weeks, after which the mice were euthanized 2 months following the initial administration of AAV5."
M6ACROT05369 "For the therapeutic intervention, AAV5/sh-circFTO or AAV5/sh-NC vectors were administered on day 21, concurrently with booster vaccination. Ankle joint cavities received an injection of 5 L of AAV5 solution containing 2 × 109 viral particles per L. This injection was repeated weekly for a duration of 5 weeks, after which the mice were euthanized 2 months following the initial administration of AAV5."
M6ACROT05370 "For the therapeutic intervention, AAV5/sh-circFTO or AAV5/sh-NC vectors were administered on day 21, concurrently with booster vaccination. Ankle joint cavities received an injection of 5 L of AAV5 solution containing 2 × 109 viral particles per L. This injection was repeated weekly for a duration of 5 weeks, after which the mice were euthanized 2 months following the initial administration of AAV5."
M6ACROT05371 "For the therapeutic intervention, AAV5/sh-circFTO or AAV5/sh-NC vectors were administered on day 21, concurrently with booster vaccination. Ankle joint cavities received an injection of 5 L of AAV5 solution containing 2 × 109 viral particles per L. This injection was repeated weekly for a duration of 5 weeks, after which the mice were euthanized 2 months following the initial administration of AAV5."
M6ACROT05372 .
M6ACROT05373 .
M6ACROT05374 .
M6ACROT05375 .
M6ACROT05376 .
M6ACROT05377 HPAF-II cells (2.0 × 106 cells/site) stably transfected with sh-EGFP or sh-UCA1 were subcutaneously injected into 4-week-old nude mice to generate xenografts. The tumor volume was measured every week after injection and calculated using the following formula: length × (width2)/2.
M6ACROT05378 "SKOV3 or A2780 cells were infected with the indicated lentiviral vectors and injected (5 × 106 cells/mouse in 200 uL volume) subcutaneously into the left armpit of 6-week-old BALB/c nude mice. After 21 days, the animals were sacrificed to confirm the presence of tumors and weigh the established tumors."
M6ACROT05379 Mice (five in each group) were injected subcutaneously with 0.1 ml of cell suspension containing 2 × 106 cells in the back flank.
M6ACROT05380 .
M6ACROT05381 "About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group)."
M6ACROT05382 "About 1× 107 cells were injected subcutaneously into the axilla of the female athymic BALB/C nude mice (4-6 weeks old, 18-22 g, five mice per group)."
M6ACROT05383 .
M6ACROT05384 .
M6ACROT05385 .
M6ACROT05386 .
M6ACROT05387 .
M6ACROT05388 .
M6ACROT05389 .
M6ACROT05390 .
M6ACROT05391 .
M6ACROT05392 .
M6ACROT05393 .
M6ACROT05394 .
M6ACROT05395 .
M6ACROT05396 "For the tumor growth model, 1 × 106 HNE1-Scrambled or HNE1-shFAM2225A 2# cells were injected into the axilla of the mice, and the tumor size was measured every 3 days."
M6ACROT05397 "The spleen in the upper left lateral abdomen of the anesthetized mice were exposed, 106 cells suspended in 20 uL phosphate-buffered saline (PBS) were injected into the distal tip of the spleen. After injection, replacing the spleen, and closing the incision."
M6ACROT05398 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT05399 "200 uL PBS containing 1× 107 cells of stable cells were subcutaneously injected into male BALB/c athymic nude mice (6-week old, 18-20 g)."
M6ACROT05400 "for the subcutaneously injected tumor model, 2 × 106 viable cells were subcutaneously injected into the flanks of mice. For the lung metastasis model, 2 × 106 viable cells were injected into the tail veins of mice. The mice were monitored for 6 weeks for lung metastasis by hematoxylin-eosin (H&E) staining."
M6ACROT05401 .
M6ACROT05402 .
M6ACROT05403 .
M6ACROT05404 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05405 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05406 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05407 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05408 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05409 "For subcutaneous transplanted model, control and miR-143-3p stable A549 cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 uL PBS + 200 uL Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient mice to investigate tumor growth."
M6ACROT05410 "Six-week-old BALB/c nude mice were purchased from the College of Veterinary Medicine, Yang Zhou University. For the xenografted tumor model, 1 × 107 HCT116 cells in 0.2 mL PBS were subcutaneously injected into BALB/c nude mice, which were randomly divided into four groups (six mice per group). The volume of the tumors was calculated with the following equation: V = 0.5 × (length × width2). For metastasis experiments, 2 × 106 cells in 0.2 mL PBS were injected into the tail vein of nude mice, which were randomly divided into four groups (six mice per group)."
M6ACROT05411 .
M6ACROT05412 "To investigate the suppressive effect of PLGA-PEG(si-LINC00958) NPs on HCC cell growth in vivo, PDX tumor models were created as described above. When the tumors developed to 50 mm3, PLGA-PEG(si-LINC00958) NPs or PLGA-PEG(siRNA control) NPs at a dose of 200 mg/kg were injected into the mice (n = 14 in each group) through the tail vein twice weekly. Treatment continued until 4 weeks later, at which point four mice in each group were sacrificed. Tumor weight and volume were recorded immediately. "
M6ACROT05413 "Mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into two groups (five mice per group) after the diameter of the xenografted tumors had reached approximately 5 mm in diameter. Xenografted mice were then administrated with PBS or DDP (3 mg/kg per day) for three times a week, and tumor volume were measured every second day."
M6ACROT05414 Used 1 × 106 SNHG3 knocked down KY-SE150 cells and NC lentivirus to inject into the right flank of mice to generate xenografts.
M6ACROT05415 Used 1 × 106 SNHG3 knocked down KY-SE150 cells and NC lentivirus to inject into the right flank of mice to generate xenografts.
M6ACROT05416 "Cells transfected with miR-186 overexpression lentivirus (Lenti-miR-186), miR-186 inhibitor (Lenti-anti-miR-186), Lenti-miR-186 & METTL3 overexpression plasmid (Lenti-METTL3), Lenti-anti-miR-186 & METTL3 shRNA (sh-METTL3) or empty lentivirus control (Lenti-NC) were subcutaneously injected into the lower flank of nude mice."
M6ACROT05417 Indicated stable 143B cells were subcutaneously injected into nude mice.
M6ACROT05418 Indicated stable 143B cells were subcutaneously injected into nude mice.
M6ACROT05419 "For the experiments, mice were injected with 5 × 106 lung cancer cells with stably expression of relevant plasmids and randomly divided into indicated groups (five mice per group). To assess the in vivo effects of cycloleucine, the xenografted tumors had reached approximately 5 mm in diameter from mice and then these xenografted mice were feed with Vehicle or cycloleucine (25 mg/kg twice weekly) and tumor volume were measured every 3 day. Tumor volume was estimated as 0.5 × a2 × b (where a and b represent a tumors short and long diameter, respectively). Mice were euthanized after 7 weeks and the tumors were measured a final time. "
M6ACROT05420 "For liver metastasis model, mice were anaesthetized and an incision was made through the skin and peritoneum to expose the spleen. 1 × 106 HCT116 cells were injected into the spleen (n = 4 each group)."
M6ACROT05421 "For liver metastasis model, mice were anaesthetized and an incision was made through the skin and peritoneum to expose the spleen. 1 × 106 HCT116 cells were injected into the spleen (n = 4 each group)."
M6ACROT05422 .
M6ACROT05423 .
M6ACROT05424 "For the unilateral ureteral obstruction (UUO) model, male C57BL/6J mice at 8 weeks of age (20-22 g body weight) were first anaesthetized with pentobarbital sodium (50 mg/kg) via intraperitoneal injection. Then, the left ureter was ligated using 3-0 silk and a left lateral incision."
M6ACROT05425 .
M6ACROT05426 .
M6ACROT05427 .
M6ACROT05428 .
M6ACROT05429 "1 × 106 HCT-116-Luc-LINC00266-1-ORF-Flag, HCT-116-Luc- LINC00266-1-5'U, or HCT-116-Luc-LINC00266-1-MT cells were subcutaneously injected into the dorsal flanks of each BALB/c nude mouse (n = 6)."
M6ACROT05430 "A longitudinal incision was made on the skin and the muscles were separated to expose the femur. A transverse osteotomy was performed in the mid-diaphysis of the femur, and the bones were stabilized by inserting a 23-gauge intramedullary needle. Equal amounts (100 uL) of phosphate-buffered saline (PBS), plasmid METTL3 and agomiR-7212-5p (10 mg/kg body weight) were locally injected into the femoral fracture site."
M6ACROT05431 .
M6ACROT05432 .
M6ACROT05433 .
M6ACROT05434 "After a median incision on the neck, the left common carotid artery (CCA), internal carotid artery (ICA), and external carotid artery (ECA) were isolated. The left CCA and the ECA were ligated."
M6ACROT05435 .
M6ACROT05436 .
M6ACROT05437 "Previously prepared U87 cells (5 × 106 cells, 60 uL) stably infected with miRNA were injected subcutaneously in the right flank of the mice, whereas control U87 cells infected with empty vector were injected in the left flank."
M6ACROT05438 "Previously prepared U87 cells (5 × 106 cells, 60 uL) stably infected with miRNA were injected subcutaneously in the right flank of the mice, whereas control U87 cells infected with empty vector were injected in the left flank."
M6ACROT05441 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
M6ACROT05442 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
M6ACROT05443 2 × 106 cells were suspended in 200 uL of serum-free RPMI 1640 for injection into each mouse.
M6ACROT05444 "To establish xenograft model, RKO cells (1 × 107) tansduced with si-control (si-NC) or si-ALKBH5 or si-ALKBH5+NEAT1 were injected subcutaneously into the right flank of the nude mice (n = 6 each group) every 5 days for 4 times."
M6ACROT05445 .
M6ACROT05446 .
M6ACROT05447 .
M6ACROT05448 A total of 1 × 107 control or KCNMB2-AS1-depleted SiHa cells were resuspended in 0.1 ml phosphate-buffered saline and inoculated into the armpit of 5-week-old male BALB/c nude mice.
M6ACROT05449 .
M6ACROT05450 In vivo myocardial I/R injury was performed by 60 min of ligation of LAD followed by 5 h of reperfusion.
M6ACROT05451 In vivo myocardial I/R injury was performed by 60 min of ligation of LAD followed by 5 h of reperfusion.
M6ACROT05452 Nude mice were subjected to a subcutaneous injection of 5× 106 control and ZFAS1 silencing CaSki cells suspended in 0.2 mL DMEM medium.
M6ACROT05453 "Thirty-two 6 week-old female nude mice divided into four groups (n = 8 per group) for injections of H1299 cells transfected with (a) pLKO.1 + pWPI, (b) shcircNOTCH1 + pWPI, (c) pLKO.1 + oeGPER and (d) shcircNOTCH1 + oeGPER."
M6ACROT05454 Stable DMDRMR knockdown (KD) and control cell lines were injected subcutaneously (s.c.; 1 × 107 cells/inoculum) into the flanks of recipient NOD/SCID/IL2Rγ -null (NSG) mice.
M6ACROT05455 .
M6ACROT05456 .
M6ACROT05457 .
M6ACROT05458 .
M6ACROT05460 .
M6ACROT05461 .
M6ACROT05462 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
M6ACROT05463 "Mice were randomly divided into three groups, and subcutaneously injected with control, circ-ARL3-silenced and circ-ARL3&miR-1305-silenced HepG2.2.15 cells."
M6ACROT05465 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
M6ACROT05466 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
M6ACROT05467 "Three-week-old BABL/c female nude mice were randomized into three groups. 5 × 106 143B cells were subcutaneously injected in mice, and the tumor volume was assessed every 2 weeks. Eight weeks after injection, the animals were killed. The xenograft tumors were harvested and the tumor volumes were calculated by the standard formula: length × width2/2."
M6ACROT05468 "The enriched mammosphere cells derived from engineered BT549 and Hs578T with silenced lncRNA KB-1980E6.3 (shKB/vector), BT549, and Hs578T with lncRNA KB-1980E6.3 knockdown combined with ectopic c-Myc (shKB/c-Myc), BT549, and Hs578T with silenced IGF2BP1 (shIGF2BP1/vector), BT549, and Hs578T with knocked down IGF2BP1 combined with ectopic c-Myc (shIGF2BP1/c-Myc), and BT549, and Hs578T/shNC/vector control cells were used in Xenograft experiments. Three doses (1 × 105, 1 × 104 and 1 × 103) of spheres derived from the engineered Hs578T and 1 × 105 of spheres derived from the engineered BT549 were subcutaneously inoculated into 4- to 6-week-old female nude mice (n = 5 per group). Mice were then treated with either bevacizumab (10 mg/kg every 3 days) to form a hypoxic tumor microenvironment or vehicle PBS to form a non-hypoxic condition."
M6ACROT05469 .
M6ACROT05470 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT05471 MCF-7 cells transfected with sh-LINC00958 or empty vector were resuspended at 2 × 107 cells/mL.
M6ACROT05472 The mice were housed under standardized conditions and received normal diet. The mice were randomly divided into 5 groups (n = 6/each group): (1) Control group: normal mice served as control; (2) TAC-HF group: mice were subjected to transverse aortic constriction (TAC) to establish HF mouse model; (3) Sham group: sham-operated mice were subjected the same surgery without the placement of a ligature; (4) Doxorubicin-HF group: mice were intraperitoneally injected with doxorubicin to construct HF mouse model; and (5) PBS: mice were intraperitoneally injected with equal PBS as control.
M6ACROT05473 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
M6ACROT05474 4-6 weeks old NOD/Shi-scid/IL-2Rgamma null (NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG)) mice (female).
M6ACROT05477 .
M6ACROT05478 .
M6ACROT05479 .
M6ACROT05480 .
M6ACROT05481 .
M6ACROT05483 "HEC-1B cells (1 × 107) transfected with pcDNA or pcDNA-FENDRR were subcutaneously transplanted into the backs of the mice. Three weeks after injection, the tumor volume of mice in the pcDNA (n = 6) and pcDNA-FENDRR (n = 6) groups was measured at one-week intervals. Eight weeks after injection, all mice were sacrificed, and the tumor tissues were collected."
M6ACROT05484 .
M6ACROT05486 .
M6ACROT05487 "In the control mice or ADR mice group, the parental or chemo-resistant K562 cells were infected with LV-shCtrl. In the ADR + shLINC00470 group, the chemo-resistant K562 cells were infected with LV- shLINC00470. These cells were injected, respectively, into these 5-week-old mice subcutaneously."
M6ACROT05488 "BALB/c nude mice (4 weeks old) were acquired from Vital River Laboratory (Beijing, China). HCT116 cells with stable circ1662 expression (2 × 106 in 100 L of PBS) were injected via the tail vein. After 45 days, the mice were sacrificed. The lung metastatic carcinoma specimens were processed into paraffin-embedded sections for subsequent H&E staining and IHC."
M6ACROT05490 "The 40 nude mice of each large group were classified into 8 small groups (n = 5) and subcutaneously injected with transfected cell suspension in the back (5 × 106 cells/mL/mouse). The length and width of tumors were recorded every 4 days, and the tumor volume = (length × width2)/2. The tumor growth curve was thereby graphed. On the 28th day of injection, mice were euthanized by neck dislocation, and the xenografts were harvested, photographed, and weighed"
M6ACROT05491 .
M6ACROT05492 "U87 cells (5 × 105) transfected with an empty vector, METTL3 shRNA, or METTL3 overexpression vector were inoculated into the right frontal node of nude mice. "
M6ACROT05493 "The mice were maintained in cages under standard environmental conditions (temperature 25 ± 2 ℃ , humidity 55 ± 5% and 12-h light/12-h dark cycle) and given free access to food and tap water. All mice were randomly divided into three groups with 6 in each group. To establish xenograft model, A549 cells (1 × 107) transfected with si-control (si-NC), si-ALKBH5 or si-ALKBH5 + RMRP were injected subcutaneously into a single side of the armpit of each mouse. "
M6ACROT05494 .
M6ACROT05495 Neonatal mouse cardiomyocytes (CMs) were blinded to isolate from 7-10 cases of postnatal 1-day old wildtype C57/B mice and cultured using Pierce Primary Cardi Page 10.omyocyte Isolation Kit (Thermo Fisher Scientific) as the manufacturer's instructions.
M6ACROT05496 .
M6ACROT05497 .
M6ACROT05498 "For the in vivo tumorigenicity assay, female BALB/c nude mice (ages 4-5 weeks) were randomly divided into two groups (n = 6/group). Calu1 cells (4 × 106) that had been stably transfected with sh-LCAT3 or scramble were implanted subcutaneously into the nude mice. Tumor growth was measured after one week, and tumor volumes were calculated with the following formula: Volume (cm3) = (length × width2)/2. After four weeks, the mice were euthanized, and the tumors were collected and weighed. For the in vivo tumor invasion assay, 1.2 × 106 scramble or shLCAT3 cells were injected intravenously into the tail vein of nude mice (n = 6/group). 1.5 mg luciferin (Gold Biotech, St Louis, MO, USA) was administered once a week for 4 weeks, to monitor metastases using an IVIS@ Lumina II system (Caliper Life Sciences, Hopkinton, MA, USA)."
M6ACROT05499 .
M6ACROT05500 "Subcutaneously injected with 5 × 106 Jurkat cells and fed under Specific Pathogen Free (SPF) conditions. One week later, tumor mass was injected once a week with Agomir NC, Agomir-211, Antagomir NC and Antagomir 211, respectively, for three weeks."
M6ACROT05501 .
M6ACROT05502 .
M6ACROT05503 .
M6ACROT05504 "For the PDX model, fresh patient HCC tissues were cut into fragments with a volume of 3 × 3 mm3 and then implanted subcutaneously into the flanks of nude mice. The mice were given sorafenib (30 mg/kg) or vehicle orally twice a week for 24 days. This procedure was approved by the Ethics Committee of Jinling Hospital."
M6ACROT05505 CircARHGAP12-stable knockdown cervical cancer cells (100 uL PBS containing 5 × 106 cells) were subcutaneously injected into the lateral flank of BALB/c nude mice.
M6ACROT05506 .
M6ACROT05507 .
M6ACROT05508 .
M6ACROT05509 .
M6ACROT05510 "Eighteen BALB/C female nude mice (SLAC Laboratory Animal Co., Ltd., Shanghai, China) aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml.For construction of the model of lung metastasis of BC, 3 × 106 MCF-7 cells infected with sh-NC + oe-NC, sh-METTL3 + oe-NC or sh-METTL3 + oe-HMGA2 were injected into nude mice via a tail vein. After 5 weeks, the pathological changes of lung tissue were observed by hematoxylin and eosin staining (HE staining) and the metastasis of tumor nodules were observed."
M6ACROT05511 .
M6ACROT05512 "Osmotic mini-pumps containing AngII (1 ug/kg/min, Enzo Bioche) were implanted in 7-week-old male mice. To interfere with the expression of KIAA1429, ALKBH5, or DDX6 in vivo, adeno-associated virus 9 (AAV9) vectors carrying a variety of overexpression plasmids or interfering RNA were randomly injected through the tail vein to C57BL/6N mice."
M6ACROT05513 "Osmotic mini-pumps containing AngII (1 ug/kg/min, Enzo Bioche) were implanted in 7-week-old male mice. To interfere with the expression of KIAA1429, ALKBH5, or DDX6 in vivo, adeno-associated virus 9 (AAV9) vectors carrying a variety of overexpression plasmids or interfering RNA were randomly injected through the tail vein to C57BL/6N mice."
M6ACROT05514 "BALB/cnu/nu mice (4-5 weeks old) were used for the xenograft experiment. The mice were randomly divided into 2 groups (n = 6 for each group) and injected with 5 × 106 HT-1197 cells in control group or FTO plasmid group, respectively."
M6ACROT05515 .
M6ACROT05516 .
M6ACROT05517 "For the PDX model, fresh patient CRC tissues were cut into fragments with a volume of 3 × 3 mm3 and then implanted subcutaneously into the flanks of nude mice. The mice were given 5-FU (30 mg/kg) or vehicle orally twice a week for 24 days. Tumor growth was measured at the indicated time points. After 24 days, the mice were sacrificed for euthanasia by intraperitoneal injection of Pelltobarbitalum Natricum (150 mg/kg). "
M6ACROT05518 "The number of cells inoculated in each mouse was 4 × 106, 1 × 106, 2 × 106 and 1 × 106, respectively."
M6ACROT05519 "The number of cells inoculated in each mouse was 4 × 106, 1 × 106, 2 × 106 and 1 × 106, respectively."
M6ACROT05520 .
M6ACROT05521 A total 5 × 106 stably transfected SiHa cells were subcutaneously injected into the flank of nude mice.
M6ACROT05522 "Nu/nu athymic nude mice (male, 3-4-weeks old) ,divided into two groups. A total of 3 × 105 MKN-45 cells, stably transfected with THAP7 antisense RNA 1 (THAP7-AS1, a novel lncRNA) vector or empty vector, were inoculated subcutaneously into the axillary fossa or lateral tail vein of the mice (n = 6 each). Tumor growth was examined every 4 days, and tumor volumes were calculated using the following equation: V (mm3) = A × B2/2"
M6ACROT05523 "LUAD cells stably HCG11 and/or LATS1 overexpressed or silenced were subcutaneously injected into the flank of the BALB/c nude mice (male, 4 weeks old). "
M6ACROT05524 "LUAD cells stably HCG11 and/or LATS1 overexpressed or silenced were subcutaneously injected into the flank of the BALB/c nude mice (male, 4 weeks old). "
M6ACROT05525 .
M6ACROT05526 .
M6ACROT05527 .
M6ACROT05528 Male C57BL/6 mice (4-6 weeks old) were used in all tumor allografting experiments and transplanted with GL261-luc cells (1× 105) into the frontal lobes of brains.
M6ACROT05529 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
M6ACROT05530 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
M6ACROT05531 "Chose 4-week-old female BALB/c nude mice for tumor xenograft experiments, which randomly were divided into four groups (n = 5 per group). Bladder cancer cells (3 × 106) were subcutaneously injected into the right axilla of the nude mice."
M6ACROT05532 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice. "
M6ACROT05533 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice. "
M6ACROT05534 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice. "
M6ACROT05535 "To create the xenograft neoplasm system, 40 male BALB/c nude mice aged 5 weeks were randomly separated into sh-NC, sh-circHPS5, sh-circHPS5+CTRL, and sh-circHPS5+SAH groups (n = 5 for each group). HCC cells were subcutaneously injected into the axilla of the nude mice. "
M6ACROT05536 "Approximately 1 × 107 stably transfected T24 cells were subcutaneously injected into BALB/c nude mice. The length (L) and width (W) of the tumours were measured weekly using callipers, while their volume was calculated using the equation: V = (L × W2)/2. After 4 weeks of injections, the mice were euthanised, and the tumour tissues were removed and weighed."
M6ACROT05537 "The 4-week-old BALB/c-nu mice (half of the female and half of the male),They were randomized into groups with approximately equivalent numbers before tumor cell inoculation. The investigator was blinded to the group allocation of the animals during the experiment; 5 × 106 HCT116 cells were injected subcutaneously into the right armpit region."
M6ACROT05538 .
M6ACROT05539 Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
M6ACROT05540 Mouse subcutaneous xenograft and lung metastasis experiments were carried out with six 4-week-old male BALB/c nude mice.
M6ACROT05541 "1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group."
M6ACROT05542 "1 × 106 (100 ul) cells of infected and uninfected by lentiviral were, respectively, injected subcutaneously into nude mice which divided randomly into scramble group and shKCNQ1OT1-1 group."
M6ACROT05543 .
M6ACROT05544 .
M6ACROT05545 .
M6ACROT05546 "For xenograft tumor model, 5-week-old male BALB/c nude mice (n = 10) were gained from Beijing Laboratory Animal Center (Beijing, China). BC cells (MCF-7/ADR, 1 × 107 cells/0.2 mL PBS:Matrigel = 1:3, v/v) were transfected with NC or sh-DLGAP1-AS1 and then subcutaneously implanted into the flank of mice. Every 3 days, the length and width were recorded and calculated for the volume: V = (width2× length × 0.52). At the indicated time, nude mice were sacrificed, and the parameters of tumor weight were detected. "
M6ACROT05547 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT05548 "Groups of HCT116-Luc-shCtrl, HCT116-Luc-shLINC00460, and HCT116-Luc-shLINC00460 + HMGA1 cells (5 × 106) were injected subcutaneously into the flanks of mice correspondingly."
M6ACROT05549 .
M6ACROT05550 .
M6ACROT05551 .
M6ACROT05552 .
M6ACROT05553 6- to 12-week-old mice were used for experiments.
M6ACROT05554 "The thoracic cavity of rats was exposed, and the left anterior descending coronary artery was ligated with a 6-0 silk thread. Successfully surgical MI could be observed, with myocardium color fading and pulse weakening. After 30 min of ischemia, the blood flow was restored by releasing the slipknot, and then 120-min perfusion was performed. Afterward, the thoracic cavity of rats was sutured. The rats were assigned into 4 groups, with 12 rats in each group. Lentivirus packaged short hairpin (sh)-negative control (NC) and sh-METTL3 (GenePharma, Shanghai, China) were injected into the rats via tail vein 24 h before operation. The titer of lentivirus was 1 × 109 TU/mL, and the injection rate was 0.2 ul/min for 10 min. Blood samples were collected 24 h after reperfusion."
M6ACROT05555 "Each mouse was anaesthetized with inhaled isoflurane, and the left proximal ureter was exposed. Then, the ureter was ligated with 6-0 silk thread and severed. In the sham operation group, the left ureters of mice were exposed, but not ligated or severed. The 3rd, 7th and 14th days after surgery were the time points for killing. At each time point, a total of 10 mice in the UUO group were executed, and a total of 10 mice in the sham group were also executed to serve as controls."
M6ACROT05556 "The normal group consisted of C57BL/6J mice on normal diet and the HFD group consisted of ApoE C57BL/6J mice on HFD (containing 10% lard oil, 4% milk powder, 2% cholesterol, and 0.5% sodium cholate). Four weeks after HFD feeding, the mice were injected with 200 ul lentivirus containing 1 × 10-/--/-8 TU (lentivirus carrying sh-METTL3 or sh-NC; designed and constructed by GENCHEM (Shanghai, China)) via tail vein. Six weeks after transfection, all mice were euthanized by a tail vein injection of 200 mg/kg pentobarbital sodium."
M6ACROT05557 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05558 "Based on our study design, the enrolled animals were blindingly grouped and received the following treatments: (1)Sham-NC vectors, (2)Sham-sh-METTL3, (3)Sham-sh-METTL3 + miR-150, (4)Sham-sh-METTL3 + Lv-YTHDF2, (5)Sham-sh-METTL3 + sh-BDNF, (6)Sham-anti-miR150, (7)Sham-anti-miR150+sh-BDNF, (8)SNI-Lv-METTL3, (9)SNI-Lv-METTL3 + anti-miR-150, (10)SNI-Lv-METTL3 + sh-YTHDF2, (11)SNI-Lv-METTL3 + Lv-BDNF, (12)SNI-miR150, (13)SNI-miR150+Lv-BDNF. The pain behaviors were detected in the respective time points and the expressions of METTL3 and miR-150 were determined at day 14 after the rats were sacrificed. "
M6ACROT05559 "HCC cells stably transfected with empty vector or circCPSF6 were subject to construct animal models, followed by the regular treatment of verteporfin, an inhibitor of YAP signaling."
M6ACROT05560 .
M6ACROT05561 "The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment."
M6ACROT05562 "The pump was prefilled with Ang-II or saline and then incubated in sterile saline at 37 ℃ for 48 h. After the mice were anesthetized with 3.0% isoflurane mixed with oxygen, an incision was made on the back skin of the mice, and the pump was implanted into the subcutaneous area, followed by suturing the incision. After the operation, the mice were given buprenorphine (0.1 mg/kg) to reach analgesia. Finally, the regaining consciousness mice were returned to cages and fed until the end of the experiment."
M6ACROT05563 The PC cell line PANC1 was subcutaneously injected into the dorsal flank of the mice at the concentration of 1 × 106 cells per mouse.
M6ACROT05564 The PC cell line PANC1 was subcutaneously injected into the dorsal flank of the mice at the concentration of 1 × 106 cells per mouse.
M6ACROT05565 "First, subcutaneous transplanted model was used to evaluate the growth of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Second, subcutaneous transplanted model was used to evaluate the metastasis potential of BT-549LMF3 and BT-549 cells. Cells (5 × 106 per mouse, n = 5 for each group) were diluted in 200 ul PBS + 200 ul Matrigel (BD Biosciences) and subcutaneously injected into immunodeficient female mice. Third, the in vivo lung metastasis model was established by injecting with BT-549, BT-549LMF3, FTO stable BT-549LMF3, sh-METTL3 BT-549LMF3, and sh-KRT7 BT-549LMF3 stable cells (1 × 106 per mouse, n = 5 for each group)"
M6ACROT05566 "For the tumor xenograft, mice were randomly divided into three groups with four mice for each group. Then, 1 × 106 cells after indicated treatment were harvested and resuspended in 50 ul of PBS. Then the cells were subcutaneously injected into the right front flank of each mouse."
M6ACROT05567 Approximately 5 × 106 253J and 5637 cells infected with indicated vectors were injected subcutaneously into the flank of the mice.
M6ACROT05568 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old)."
M6ACROT05569 "circ_0008542, where rats were injected with exosomes containing circ_0008542 into the tail vein for 8 weeks."
M6ACROT05570 "circ_0008542, where rats were injected with exosomes containing circ_0008542 into the tail vein for 8 weeks."
M6ACROT05571 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
M6ACROT05572 "At 1 week post-injection with PC-3 cells, mice were randomly assigned to three groups (n = 8 per group): the ASO-NC group (injection with ASO negative control targeting unknown sequence, 5 nmol in 100 uL PBS for each mouse), the ASO-L group (injection with low-dose ASO targeting PCAT6, 5 nmol in 100 uL PBS for each mouse), and the ASO-H group (injection with high-dose ASO targeting PCAT6, 10 nmol in 100 uL PBS for each mouse)."
M6ACROT05573 "Fresh PDX tumor samples collected from two established PDX models (PDX #07 with high TRIB2 expression and PDX #12 with low TRIB2, passages three to four) were minced and subcutaneously implanted into the flanks of 3- to 4-week-old female BALB/c nude mice (Jiesijie Laboratory Animals)."
M6ACROT05574 BALB/c nude mice which were co-injected with THP-1 cells and PATU-8988 cells subcutaneously.
M6ACROT05575 "5 × 105 cells were injected subcutaneously into the right axilla of mice. Tumor volume was measured by a caliper weekly and calculated as length × width2 × 0.52. For the liver orthotopic-implanted models, each liver of mice was injected with 1 × 106 cells. "
M6ACROT05576 A total of 106 LLC cells transfected with the following constructs were injected subcutaneously into C57BL/6 mice.
M6ACROT05577 A total of 106 LLC cells transfected with the following constructs were injected subcutaneously into C57BL/6 mice.
M6ACROT05578 The cells (1 × 106) were re-suspended in normal saline and mixed with 25% Matrigel matrix (50 uL) at a 1:1 ratio and subcutaneously injected into the right groin of the mice.
M6ACROT05579 .
M6ACROT05580 "All the mice were randomly divided into three groups: the normal saline group (NS), the lipopolysaccharide + negative control group (LPS + NC), and the LPS + miR-29a-3p agomir group (LPS + Agomir)."
M6ACROT05581 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
M6ACROT05582 "For subcutaneous xenograft models, 0.1 mL of cell suspension containing 106 cells were injected subcutaneously into the right flank of mice (n = 6 for each group)."
M6ACROT05583 "For melittin treatment study, 4-week-old female BALB/c nude mice were subcutaneously injected with 1 × 107 T24 or BIU87 cells."
M6ACROT05584 .
M6ACROT05585 "Established a xenograft model in BALB/c nude mice by inoculating HCC827-GR cells transfected with the constructs for circASK1 silencing, ASK1-272a.a overexpression and ASK1-272a.a overexpression/circASK1 knockdown"
M6ACROT05586 "Established a xenograft model in BALB/c nude mice by inoculating HCC827-GR cells transfected with the constructs for circASK1 silencing, ASK1-272a.a overexpression and ASK1-272a.a overexpression/circASK1 knockdown"
M6ACROT05587 .
M6ACROT05588 .
M6ACROT05589 "In vivo animal assay, FOXD2-AS1 overexpression promoted the tumor growth in mice subcutaneous injection"
M6ACROT05590 "For the xenograft tumor model, approximately 1 × 106 ccRCC cells suspended in 100 uL PBS were subcutaneously inoculated in the right flank of 5-week-old BALB/c nude mice. For the ccRCC orthotopic implantation model, approximately 1 × 106 ccRCC cells suspended in 30 uL Matrigel were injected under the renal capsule of 5-week-old BALB/c nude mice. After 6 weeks, the anesthetized mice were intraperitoneally injected with D-luciferin (Yeason) and imaged using an in vivo imaging system to detect tumor growth and metastasis. For the lung metastasis model, approximately 5 × 105 ccRCC cells suspended in PBS were injected into the tail vein of 5-week-old mice. After 6-8 weeks, mice were anesthetized and lung metastasis was imaged as above. "
M6ACROT05591 .
M6ACROT05592 .
M6ACROT05593 "Mice were divided into two groups (n = 4/group) randomly. 3× 106 cells suspended in 200 uL PBS were administered via subcutaneous injection over the right flank region of nude mice. After the development of palpable tumors (average volume, 50 mm3), intratumoral injection of synthetic miR-193b, or negative control complexed with siPORT Amine transfection reagent (Ambion, USA) was given 6 times at a 4-day interval."
M6ACROT05595 .
M6ACROT05596 .
M6ACROT05597 .
M6ACROT05598 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (SLAC, Shanghai, China). SGC-7901 cells (1 × 107) transfected with the METTL14 overexpression vector or no-load lentivirus vector were resuspended in 200μL of sterile PBS and injected subcutaneously into the right flanks of mice. After 4 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis. Tumor volume was calculated using formula: volume = (length x width2)/2. The animal experiments were approved by the Ethics Committee of Shanghai Sixth People's Hospital."
M6ACROT05599 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (SLAC, Shanghai, China). SGC-7901 cells (1 × 107) transfected with the METTL14 overexpression vector or no-load lentivirus vector were resuspended in 200μL of sterile PBS and injected subcutaneously into the right flanks of mice. After 4 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis. Tumor volume was calculated using formula: volume = (length x width2)/2. The animal experiments were approved by the Ethics Committee of Shanghai Sixth People's Hospital."
M6ACROT05600 .
M6ACROT05601 .
M6ACROT05602 .
M6ACROT05603 "BALB/c male nude mice were 4 weeks old. GBM cells with stable overexpression or knockdown of FTO and ovNC or shNC were transduced with lentivirus expressing luciferase. The cells were intracranially injected at a density of 5 × 105/10 uL into every mouse to form an orthotopic xenograft model. Coordinates of injection were 1 mm anterior and 2.5 mm right to the bregma, at a depth of 3.5 mm (the right frontal lobes of the mouse). Every 6 days, bioluminescence imaging (IVIS Lumina Series III; PerkinElmer, Waltham, MA) was used to image the mouse. At 8 days, we randomly chose 5 mice from each group to euthanize them, and their brain tissues were fixed with paraformaldehyde for further study. Another 5 mice were used for survival time analysis. For DB2313 (563801; MedKoo) anti-tumor research, male nude mice were subcutaneously injected with 5 × 106 U87MG cells suspended in 0.1 mL PBS. After 7 days, mice were intraperitoneally injected with DB2313 at density of 10 mg/kg/day dissolved in PBS solvent containing 10% DMSO for 7 days. The other group treated with vehicle only was set as the control group."
M6ACROT05604 "BALB/C nude mice (5 weeks old, weighing 18-22 g) were fed in specific pathogen-free facilities and subcutaneously inoculated with U266 cells (1 × 106). The mice were randomly divided into 3 groups with 6 mice per group, when the tumor was measurable. Then, miR-27a-3p mimic or sh-METTL3 was injected intratumorally at an interval of 4 days a total of 4 times. Tumor volume was measured using a digital caliper every week and calculated using the formula V = 1/2 (width2 × length). "
M6ACROT05605 .
M6ACROT05606 "An incision was made in the skin along the medial dorsal line to the aponeurotic and muscular planes, and the posterior vertebral arches were exposed from T8 to T12. Under the dissection stereomicroscope, 3-mm-long laminectomy was performed on the caudal end of T10 vertebra and the rostral end of T11 vertebra. The Infinite Horizons impactor (Infinite Horizons, L.L.C., Lexington, KY, USA) was adopted to produce the contusion SCI using a force of 60 kdyn/cm2. The SCI model rats were established and randomly assigned to SCI model group, ant-NC (negative control, SCI rats treated with lentiviral (lv)-shRNA NC of Mettl14) group and ant-Mettl14 group (SCI rats treated with lv-shRNA of Mettl14). Rats were subjected to laminectomy and then treated with lv-shRNA Mettl14/lv-shRNA-NC (50 ul/day, 100 nmoL/mL; RiboBio, Guangzhou, China) via an intrathecal injection through lumbar puncture for 3 days (0, 1, and 2 days) after 15 min of SCI modelling. In addition, the unmodeled rats were set as sham group."
M6ACROT05607 "Male C57BL/6J mice were anesthetized with an intraperitoneal injection of pentobarbital (40 mg/kg). The abdominal aorta between the renal arteries and the bifurcation of the iliac arteries was disassociated from the surrounding structures. Video microscopy was used to assay the diameter of the aorta in triplicate. After the measurements were taken, a small piece of gauze dipped in 0.5 mol/L CaCl2 was spread perivascularly onto the aortic passage for 15 min. Control mice received substitute treatment with NaCl (0.9%)-soaked gauze for 15 min. Then, the aorta was rinsed with 0.9% sterile saline, and the incision was sutured. After 3 or 6 weeks, all the animals were sacrificed, and the aortas were harvested for further analysis."
M6ACROT05608 "Eighteen BALB/C female nude mice aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml. The prepared cell suspension was injected into the left armpit of the mice, and the subsequent tumor growth was recorded."
M6ACROT05609 .
M6ACROT05610 "Eighteen BALB/C female nude mice aged 4-5 weeks and weighing 15-18 g were randomly assigned into three groups of six mice. The MCF-7 cell lines stably transfected with sh-NC + oe-NC, sh-METTL3 + oe-NC and sh-METTL3 + oe-HMGA2 were selected for subcutaneous establishment of the BC cell line MCF-7 as xenografts in the nude mice. For this purpose, MCF-7 cell lines in the logarithmic growth stage were prepared into a suspension with a concentration of about 1 × 107 cells/ml. The prepared cell suspension was injected into the left armpit of the mice, and the subsequent tumor growth was recorded."
M6ACROT05611 .
M6ACROT05612 .
M6ACROT05613 "Once the tumor volume increased to about 1 cm3, six groups of MCF7 bearing mice (n = 10 in each group) were injected with PBS (0.1 ml, caudal vein) and adriamycin (0.1 ml, 10 mg/kg), respectively. When the tumor reached 1.5 cm in any direction (defined as event-free survival analysis), 10 mice in each group were selected to measure the tumor size and weight on the 12th day after adriamycin injection."
M6ACROT05614 "Ten four-week-old BALB/c nude mice were injected with LINC00958-overexpressing or vector-transfected cells. Briefly, 5 × 106 cells were subcutaneously injected in the flank of mice. Four weeks after injection, the mice were sacrificed and examined by weighting."
M6ACROT05615 "A tumor-bearing model was established by subcutaneously injecting 100 ul HT29 cells (5× 106) followed by an intravenous injection of CAFs-derived exosomes (50 ug/mouse every three days) into the tail vein of the mice. An intraperitoneal injection of 5-FU (50 mg/kg, every week) was administered on day 12. "
M6ACROT05616 "For the mouse model of miR-320a-3p overexpression, a total of 24 male C57BL/6 mice were divided randomly into four groups (n = 6 in each group): saline, silica, silica plus AAV9-miR-NC, and silica plus AAV9-miR-320a-3p. The mice in the silica plus AAV9-miR-NC/AAV9-miR-320a-3p groups were anesthetized using the same method, then administered intratracheally 50 uL AAV9-miR-NC/AAV9-miR-320a-3p per mouse at a titer of 8 × 1012 v. g./ml. Three weeks later, these mice were treated in the same way using anesthesia, saline, and silica as mentioned above. Subsequently, after 4 weeks, the mice were sacrificed, and the lungs were isolated and frozen at -80 ℃ for further study."
M6ACROT05617 A549 cells (1 × 106 cells) were intraperitoneally injected into the abdomen of nude mice. Tumor length and width were measured and recorded every 4 days after inoculation. Tumor volume was calculated as 1/2 × length × width2. The tumor weight was weighed and recorded 20 days after inoculation.
M6ACROT05618 "H1299 cells were transfected with sh-ABHD11-AS1 and harvested from six-well plates, then resuspended at a density 1 × 107 cells/ml. Mice were subsequently injected with 100 uL suspension at the right flank. After injection, tumor weight and length were examined every 3 days."
M6ACROT05619 "10-week-old male C57BL/6 mice were randomly divided into six groups: normal group (n = 5); MIA group (n = 5); MIA + agomir-NC group (n = 5); MIA + agomir-328-3p group (n = 5); MIA + AAV-NC group (n = 5); and MIA + AAV-AC008 group (n = 5). For induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich) in the knee. One week later, agomir-328-3p or negative control agomir (RiboBio) and adeno-associated virus expressing AC008 (AAV-AC008) or negative control virus (GeneChem, Shanghai, China) were injected into the knee joints of mice in the appropriate groups. Treatments were administered at 20 μL per joint per mouse once per week for 5 consecutive weeks. Six weeks later, the mice were euthanized, and the knee joints were collected for safranin-O/fast green staining and IHC analysis."
M6ACROT05620 "For the tumorigenicity studies, 3 × 106 HCC827 cells stably expressing sh-LINC01833 or sh-NC were injected subcutaneously into the ventral side of male BALB/c nude mice in the according groups with five mice in each group. Five mice were sampled each time. Tumor size and weight were examined at 28 days after injection."
M6ACROT05621 "A total of 32 BALB/c nude mice (male, 5-week-old) were chosen and assigned to four groups: (1) Control group (injected with XPTS-1 cells), (2) sh-NC (injected with XPTS-1 cells with sh-NC transfection), (3) sh-ALKBH5 (injected with XPTS-1 cells with sh-ALKBH5 transfection) and (4) sh-FOSL1 group (injected with XPTS-1 cells with sh-FOSL1 transfection) (8 mice/group). The third-generation XPTS-1 cells were used for tumor induction. 0.2 mL of the above cell suspensions containing 2 × 104 cells were injected into the back of each mouse."
M6ACROT05622 .
M6ACROT05623 .
M6ACROT05624 "Lentiviruses containing sh-METTL-14 and its negative control (RiboBio Co., Ltd., Guangzhou, China) were transduced into CAL27 cells and stably transduced cells were screened using puromycin. CAL27 cells (3 × 106 cells/mouse) were subcutaneously inoculated into the posterior flank of each mouse (N = 12/group). "
M6ACROT05625 "All female BALB/c nude mice (3-4 weeks old) used in our study were purchased from the Beijing Vital River Laboratory Animal Technology Co., Ltd. and then housed in specific pathogen-free units. PDX modeling in BALB/c nude female (male, 4-5 weeks old) and in vivo assays were performed as in prior studies "
M6ACROT05626 "To generate an AMI mouse model, mice were anesthetised by intraperitoneal injection of sterile pentobarbital sodium at 50 mg/kg body weight."
M6ACROT05627 "Female BALB/c nude mice (ages 4-5 weeks, 18-20 g) were purchased from the Charles River Laboratories. For the tumor growth model, 1 × 106 HONE-1 sh-NC or sh-ZFAS1 cells were injected into the axilla of the mice, and the tumor size was measured every 3 days. On day 30, the mice were killed, and the tumors were dissected and weighed."
M6ACROT05629 "Forty-eight 4-week-old, specific pathogen-free female BALB/c nude mice (weighing 20-22 g),TPC-1 cells were subjected to 3 different treatments: transfection with sh-NC or sh-METTL3 + sh-NC or sh-METTL3 + sh-STK4. For the tumor formation model, 24 nude mice were randomly divided into 3 groups (8 mice/group). The aforementioned cell suspension of the 3 groups was diluted to a concentration of 1 × 107 cells/mL, and 0.2 mL of each group was subcutaneously administrated to the right groin of the nude mice for the development of xenografted tumors. Five weeks later, the mice were euthanized with an intraperitoneal injection of 40 mg/kg pentobarbital (Sigma) to collect the tumor tissues."
M6ACROT05630 "The mice were fed in an SPF environment (cycle of 12-h light and 12-h dark) with a free diet. All the mice were adaptively fed for 5 days before experiments and randomly divided into the following four groups: the siNC+MC group (n = 10), the METTL3 siRNA1+MC group (n = 10), siNC+M group (n = 10) and the METTL3 siRNA1+M group (n = 10). Then, the right forelimb of mice in the siNC+MC group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with siNC and MC; the right forelimb of mice in the METTL3 siRNA1+MC group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with METTL3 siRNA1 and MC; the right forelimb of mice in the siNC+M group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with siNC and M; the right forelimb of mice in the METTL3 siRNA1+M group was subcutaneously injected with 100 uL PBS containing 1 × 106 SK-Hep1 cells co-transfected with METTL3 siRNA1 and M."
M6ACROT05631 "Mice (male and 6 weeks old) were subcutaneously injected with NSCLC cells (1.0*106 cells/200 uL). The mice were terminated after 4 weeks of induction, and the tumor volume and tumor weight were measured."
M6ACROT05633 "Control vector, TUSC7 knockout, FLI-06 treated H1975 cells (1*107) cells were suspended in 100 uL of serum-free DMEM medium (Hyclone, USA), mixed with matrix gel (Corning, USA), and then were injected subcutaneously. The changes in the tumor size were recorded every 3 or 5 days."
M6ACROT05634 "To study the effect of miR-30d on liver metastasis of PDACs, 5 × 106 cells in 50 uL of PBS were injected into the spleens of nude mice (6 mice per group). Anesthetized mice were injected intraperitoneally with D-luciferin (150 mg/kg) every other week and imaged using an IVIS 100 imaging system (Xenogen, CA, USA) 10 min after the injection. The mice were sacrificed and their liver metastases were checked by standard histological examination 8-9 weeks after injection."
M6ACROT05635 "Luciferase-labeled KYSE150 cells (5 × 106) were inoculated into the footpads of BALB/c nude mice (4-5 weeks old, 18-20 g) to establish the popliteal lymphatic metastasis model."
M6ACROT05637 "IMQ-induced psoriatic model was constructed by applying 10 mg per ear 5% IMQ for 8 consecutive days, and 6 ug macrophage-specific control or hsa_circ_0004287 plasmid was topically applied every 2 days (5 mice per group per experiment)."
M6ACROT05638 "IMQ-induced psoriatic model was constructed by applying 10 mg per ear 5% IMQ for 8 consecutive days, and 6 ug macrophage-specific control or hsa_circ_0004287 plasmid was topically applied every 2 days (5 mice per group per experiment)."
M6ACROT05639 .
M6ACROT05640 "To detect the effect of NKILA on CAA growth, 5 × 106 control and NKILA-depleted HuCCT1 cells (n = 4 per group) were subcutaneously injected into male BALB/c nude mice (4-6 weeks old)."
M6ACROT05641 "BACE1-AS knockout SW620 or parental SW620 cells were injected into the inferior Hemi-spleen of the mice. The weights of mice were recorded every 3 days. After 7 weeks, the mice were euthanized. The whole livers of mice were resected and photographed to assess metastatic burden.
"
M6ACROT05642 .
M6ACROT05643 .
M6ACROT05644 .
M6ACROT05645 .
M6ACROT05646 "Animal experiments were approved by the Hainan Affiliated Hospital of Hainan Medical University. Male BALB/c mice (Vital River, Beijing, China) were subcutaneously injected with H322 cells (1 × 106) transfected with ov-NC, ov-FTO, ov-GAS5 or ov-FTO + ov-GAS5 (n = 5/group). Tumor volume was measured every 7 days, and mice were sacrificed and the tumor tissues were removed for weighting and analyzing after 35 days. Also, tumor tissues were prepared into paraffin sections to carry out Ki67 immunohistochemistry (IHC) staining using SP Kit (Solarbio, Beijing, China) and TUNEL staining using Colorimetric TUNEL Apoptosis Assay Kit (Beyotime) basing on kit instructions."
M6ACROT05647 "Before hyperoxia treatment, mice in the BPD/PVT1 KO group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 at a titer of 1 × 109 pfu/100 μL. Mice in the BPD/PVT1 KO + IL-33 group were intratracheally instilled with 5 μL adenovirus vector expressing sh-PVT1 and 5 μL adenovirus vector expressing IL-33."
M6ACROT05648 .
M6ACROT05649 .
M6ACROT05650 "for the TAA (Sigma-Aldrich, USA) induced mouse liver fibrosis model, briefly, TAA (200 mg/kg, diluted in saline) was injected 3 times weekly for 8 weeks. Mice were sacrificed 24 h after the last administration."
M6ACROT05651 .
M6ACROT05652 "Adult, male C57BL/6J mice (9-10 weeks old, 20-25 g) supplied from the Animal Resources Center were used for experiments. Mice were housed 4 animals per cage on a 12 h light:dark cycle (lights on 0700 h) in a humidity- and temperature-controlled vivarium, with rodent chow and water provided ad libitum."
M6ACROT05653 "Adult, male C57BL/6J mice (9-10 weeks old, 20-25 g) supplied from the Animal Resources Center were used for experiments. Mice were housed 4 animals per cage on a 12 h light:dark cycle (lights on 0700 h) in a humidity- and temperature-controlled vivarium, with rodent chow and water provided ad libitum."
M6ACROT05654 .
M6ACROT05655 "Indicated HCC cells were intrasplenically injected into 5-week-old male BALB/C athymic nude mice (SpePharm Biotechnology, Beijing, China). After being fed in specific pathogen free condition for 35 days, the mice were euthanized and the livers were resected and subjected to HE staining."
M6ACROT05656 "Indicated HCC cells were intrasplenically injected into 5-week-old male BALB/C athymic nude mice (SpePharm Biotechnology, Beijing, China). After being fed in specific pathogen free condition for 35 days, the mice were euthanized and the livers were resected and subjected to HE staining."
M6ACROT05657 "Pregnant rats were randomly assigned to 5 groups: normal pregnancy (normal) group, PE group, circSETD2 overexpression (PE + oe-circSETD2) group, METTL3 overexpression (PE + oe-METTL3) group, overexpression control (PE + oe-NC) group, joint treatment (PE + oe-METTL3 + si-circSETD2) group, and joint treatment control (PE + oe-METTL3 + si-NC) group, with 6 rats in each group. After continuous injection of L-NAME for 4 days, the rats in the overexpression and overexpression control groups were further injected with 30 μl lentivirus vector oe-circSETD2, oe-METTL3 or oe-NC solution (GenePharma, Shanghai, China) through the tail vein; after continuous injection of L-NAME for 4 days, the rats in the PE + oe-METTL3 + si-circSETD2 group were further injected with 30 μl lentiviral vector oe-circSETD2 and si-circSETD2 (GenePharma) at the virus titer of 5 × 107 PFU/mL. The rats were euthanized on the 16th day of pregnancy. Then, the chorionic trophoblast tissues were isolated from the placenta of pregnant rats and divided into 2 parts, with 1 part for extraction and detection of tissue RNA, and the other part fixed in the buffer containing 10% formalin, embedded in paraffin, and made into 5 μm sections."
M6ACROT05658 "Pregnant rats were randomly assigned to 5 groups: normal pregnancy (normal) group, PE group, circSETD2 overexpression (PE + oe-circSETD2) group, METTL3 overexpression (PE + oe-METTL3) group, overexpression control (PE + oe-NC) group, joint treatment (PE + oe-METTL3 + si-circSETD2) group, and joint treatment control (PE + oe-METTL3 + si-NC) group, with 6 rats in each group. After continuous injection of L-NAME for 4 days, the rats in the overexpression and overexpression control groups were further injected with 30 μl lentivirus vector oe-circSETD2, oe-METTL3 or oe-NC solution (GenePharma, Shanghai, China) through the tail vein; after continuous injection of L-NAME for 4 days, the rats in the PE + oe-METTL3 + si-circSETD2 group were further injected with 30 μl lentiviral vector oe-circSETD2 and si-circSETD2 (GenePharma) at the virus titer of 5 × 107 PFU/mL. The rats were euthanized on the 16th day of pregnancy. Then, the chorionic trophoblast tissues were isolated from the placenta of pregnant rats and divided into 2 parts, with 1 part for extraction and detection of tissue RNA, and the other part fixed in the buffer containing 10% formalin, embedded in paraffin, and made into 5 μm sections."
M6ACROT05659 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
M6ACROT05660 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
M6ACROT05661 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
M6ACROT05662 .
M6ACROT05663 "Indicated HCC cells were subcutaneously inoculated into the flank of 5-week-old male BALB/C athymic mice, which were purchased from Shanghai SLAC Laboratory Animal Co. and fed in specific pathogen-free condition. Subcutaneous tumor volumes were measured every week and calculated using the formula 0.5×length×width2. At the 28th day after inoculation, subcutaneous tumors were resected, weighed, and photographed. This study was approved by Affiliated Hospital of Youjiang Medical University for Nationalities Institutional Review Board."
M6ACROT05664 .
M6ACROT05665 .
M6ACROT05666 .
M6ACROT05667 "After approval by Ethics Committee of School of Public Health, Southeast University, BALB/c nude mice (4-5 weeks old) were purchased and raised in SPF animal house. 1 × 107 HeLa cells or NC cells with stable overexpression of CARMN were prepared in each nude mouse. The mice were killed 3 weeks later, and tumor volume was measured."
M6ACROT05668 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
M6ACROT05670 .
M6ACROT05671 "15 L of an empty vector-carrying lentivirus (5 × 107 copies/mL) was administered to the control group. The lentivirus as an in vivo delivery system can carry a luciferase transgene. The delivery system was injected into the marrow cavity of the femur 4 times at week 1, and luciferase expression in live mice was followed with a whole-animal fluorescence imaging system (IVIS Spectrum, USA) at 3 weeks post-injection."
M6ACROT05672 "Nude (nu/nu) mice (4-5 weeks old) were purchased from Harlan Laboratories (Indianapolis, IN, USA). All animal studies were conducted in accordance with NIH animal use guidelines and a protocol approved by the UMMC Animal Care Committee. MIA PaCa-2 cells with LINC00901 overexpression or vector control (pCDH); LINC00901 KO or vector control (LCV2-m) at the exponential stage were harvested and mixed with 50% matrigel, and then injected into mice (2.5 million cells/spot) s.c. as described previously.21 Tumor growth was measured every 4 days, starting 2 weeks after injection of tumor cells."
M6ACROT05673 .
M6ACROT05674 "Male four-week-old BALB/C nude mice were inoculated subcutaneously in the right axilla with 1×106 HNRNPA2B1 knockout or negative control DU145 cells. Xenograft tumor growth was recorded weekly by measuring the width (W) and length (L) of the tumor with vernier calipers and calculating the tumor volume (V, mm3) using the formula V = W2 × L × 0.52. Xenograft tumors were harvested and weighed five weeks after cell injection."
M6ACROT05675 .
M6ACROT05676 "Male nude mice (6 weeks old) were purchased from the Shanghai Laboratory Animal Central (Shanghai, China). 95D cells (1 × 107) transfected with sh-HNRNPA2B1 or sh-NC lentiviruses were injected subcutaneously into the right flanks of mice. After 8 weeks, the mice were sacrificed, and the xenografted tumors were collected for hematoxylin-eosin (HE) staining and IHC analysis. "
M6ACROT05677 "Stable cell clones, at a density of 5000 cells, were subcutaneously injected into the right flank of six-week-old male nude (nu/nu) mice (SLAC, Shanghai, China). These clones were infected with SKOV3-Nc and SKOV3-shALKBH5 in 100 uL of sterilized phosphate-buffered saline. Mice were allowed to recover for six weeks before reinjection. In each group, the tumor weights of six mice were measured and recorded. All mice were euthanized within six weeks post-surgery, following the removal of their tumors. Tumor sizes were measured using Vernier calipers, with the volume calculated as 1/2 length × width^2. "
M6ACROT05678 .
M6ACROT05679 "Mice were acclimatised at the Centenary Institute Animal Facility for a minimum of 7 days after initial arrival. Prior to tumour injection, mice were anaesthetised by ketamine/xylazine by intraperitoneal injection. Subsequently, the fur surrounding the 4th mammary fat pad on the right was removed using the hair removal cream. MDA-MB-231 cells (5 × 106) in 100 L of 1:1 HBSS:Matrigel were then injected subcutaneously into the 4th right mammary fat pad using 27 g insulin needles. Mice were injected intraperitoneally with the reversal atipamezole to improve the recovery from anaesthesia. Mice were monitored twice weekly by assessing body condition, measuring body weights and tumour sizes. The frequency of monitoring was increased to daily when tumours reached > 500 mm3 in size. Mice were killed when tumours reached > 1000 mm3 in size and the relevant organs were harvested for analysis."
M6ACROT05680 .
M6ACROT05681 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05682 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05683 .
M6ACROT05684 .
M6ACROT05685 .
M6ACROT05686 "Male BALB/c nude mice (5-6 weeks) were obtained from Slac Laboratory Animal Center (Shanghai, China) and maintained under pathogen-free conditions. PANC-1 cells (2 × 106 cells suspended in 100 μl PBS) transfected with circMYO1C knockdown (sh-circMYO1C) or controls (sh-NC) were subcutaneously injected into the flank of nude mice. One week later, the tumor size was measured every three days."
M6ACROT05687 "The mice were randomly separated into four groups (n = 3-5): young mice group (YNG); naturally aging mice group (OLD); PBS-treated mice group (Con); D-gal-treated aging mice group (D-gal). YNG group was the directly purchased mice (8-week-old) actually. OLD group: mice bought continued to be raised in a specific pathogen-free facility (SPF) individually for 24 months. Mice in Con group were injected subcutaneously with phosphate buffered saline (PBS) solution formulated from powder, with the same volume as the D-gal group. Mice from D-gal group were made by injecting (D-gal, 500 mg/kg body weight) daily for 8 weeks, subcutaneously. All animals were kept under a standard environmental condition (25 ± 2 °C; 12-h light-dark cycle) and allowed free access to water and regular sterile chow diets. All animal studies were designed in accordance with ARRIVE guidelines and experiment protocols followed NIH guidelines."
M6ACROT05688 "A375 cells (1 × 107) transfected with sh-METTL3 or shNC lentiviruses were subcutaneously injected into the right flank of the mice. After 33 days, the mice were sacrificed and the xenografted tumors were collected for immunohistochemistry analysis. Animal experiments were approved by the Ethics Committee of Shanghai East Hospital (K-KYSB-2020-0)."
M6ACROT05689 .
M6ACROT05690 .
M6ACROT05691 "The male C57BL/6 mice (SPF, Eight-week-old) were randomly divided into four groups: sham (n = 6), model (n = 6), model + adeno-associated virus-negative control (AVV-shNC) (n = 6), and model + AVV-shWTAP groups (n = 6). After acclimating for one week, mice in other groups were treated with DMM surgery to induce OA as previously described except for the sham groups [19]. The mice in sham group were underwent only the skin of the right knee joint incision. The AAV-shNC and AAV-shWTAP were constructed by HANBIO (Shanghai, China). The AAV-shNC and AAV-shWTAP groups were intra-articularly injected with 10 μL of AAV-shNC and AAV-shWTAP (1 × 1013 vg/ml) through the medial parapatellar area at two weeks after the DMM operation, respectively. "
M6ACROT05692 "Thirty female BALB/c nude mice weighing 18-22 g were randomly assigned to six groups. PC9-Mock, PC9-circFUT8, A549-sh-scramble, and A549-sh-circFUT8 cells were prepared as a suspension of 4 × 105 cells in 200 μL saline, respectively, and injected into the tail vein. Mice were sacrificed at 6 weeks post injection and examined microscopically by hematoxylin and eosin (H&E) staining for the development of lung metastases."
M6ACROT05693 "Sixteen SPF female BALB/c nude mice (6 weeks old, weighing 20-22 g) (SLAC Laboratory Animal, Shanghai, China) were randomly arranged into the sh-NC group and the sh-METTL3 group, with 8 mice per group. After transfected with sh-NC and sh-METTL3, respectively, cells were rinsed with PBS to remove excess medium, detached with 0.25% trypsin, and detachment was terminated with complete medium, followed by centrifugation to collect cell precipitates. Appropriate amounts of physiological saline were added and gently pipetted into single-cell suspensions for cell counting. A total of 3 × 106 cells were resuspended in 50 μL normal saline, mixed with 50 μL 25% Matrigel (1:1), and inoculated subcutaneously into the right inguinal of nude mice. Tumor growth curves were plotted with 7, 14, 21, and 28 days as time points, and the short diameter (a) and long diameter (b) of the tumor were determined using a vernier caliper to estimate the tumor volume V = (a2b)/2. Tumor mass was weighed on a scale. Following 4 weeks, nude mice were euthanized by an intraperitoneal injection of 150 mg/kg pentobarbital (P3761, Sigma). The skin near the tumor was cut short using surgical scissors, and the tumor was separated using ball pointed pliers. The tumors were partially embedded with paraffin for histological examination, and the remaining part was kept at 80°C."
M6ACROT05694 .
M6ACROT05695 "A total of 1 × 106 HuCCT1-sgNC and HuCCT1-sgMETTL5 cells in 0.2 mL of PBS were separately injected into 6-week-old male NCG mice (N = 7 per group) via caudal vein. Mice were sacrificed at 4 weeks after injection, and the lung tissues were processed into 4-mm-thick paraffin-embedded sections. H&E staining was subsequently performed to determine the pulmonary metastasis."
M6ACROT05698 .
M6ACROT05699 "For the tumor growth study, nude mice were subcutaneously inoculated into the flanks with PC cells (3 × 106) suspended in 0.1 mL PBS. Tumor volumes were calculated as 1/2 (length × width2) weekly. After 4 weeks, the xenograft tumors were harvested for analysis. To examine the role of the FAK inhibitor in tumor growth, nude mice were inoculated subcutaneously into the flanks with 3 × 106 stable cells suspended in 0.1 mL PBS. After 1 week, pre-established tumor xenografts were treated with Y15 (0.5 mg/kg, 2 ×/week × 3) (MCE, New Jersey, China). After another 3 weeks, the xenograft tumors were harvested for analysis.
In the metastasis model, PC cells (2 × 106) in 0.1 mL PBS were injected into nude mice through their tail veins. Six weeks later, the lungs were removed for the evaluation of lung metastasis using hematoxylin and eosin (H&E) staining."
M6ACROT05700 "For the tumor growth study, nude mice were subcutaneously inoculated into the flanks with PC cells (3 × 106) suspended in 0.1 mL PBS. Tumor volumes were calculated as 1/2 (length × width2) weekly. After 4 weeks, the xenograft tumors were harvested for analysis. To examine the role of the FAK inhibitor in tumor growth, nude mice were inoculated subcutaneously into the flanks with 3 × 106 stable cells suspended in 0.1 mL PBS. After 1 week, pre-established tumor xenografts were treated with Y15 (0.5 mg/kg, 2 ×/week × 3) (MCE, New Jersey, China). After another 3 weeks, the xenograft tumors were harvested for analysis.
In the metastasis model, PC cells (2 × 106) in 0.1 mL PBS were injected into nude mice through their tail veins. Six weeks later, the lungs were removed for the evaluation of lung metastasis using hematoxylin and eosin (H&E) staining."
M6ACROT05701 .
M6ACROT05702 .
M6ACROT05703 "Male BALB/c-nude mice (5-week-old) were used in this study. First, the Antigo miR-1908-5p (Antigo-miR) for knocking down miR-1908-5p as well as its negative control (Antigo-NC) were acquired from GenePharma (China). Antigo-miR and Antigo-NC were each introduced into C666-1 cells which were then delivered through the tail of every mouse (n = 3 per group). Thereafter, all mice were raised well, and the long diameter and short diameter of tumors were measured every week. The tumor volume was calculated as tumor volume (mm3) = (short diameter)2 × long diameter/2. After 5 weeks, the mice were euthanized, and their tumors were weighed. All animal protocols were followed in accordance with the Institutional Animal Care and Use Committee of our hospital."
M6ACROT05706 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
M6ACROT05707 "Stably transfected cell lines were created by silencing circRNF13 in CC SiHa cells. Once xenografts were established, the tumors reached an approximate volume of 200 mm3. A single dose of 15 Gy irradiation was administered to female BALB/c nude mice (4-5 weeks old) in the murine model. The tumor volume was measured and recorded using vernier calipers every five days after irradiation. After 30 days, the mice were euthanized under anesthesia, and tumor tissue was collected for further investigations."
M6ACROT05708 .
M6ACROT05709 .
M6ACROT05710 "Briefly, the spine was exposed through an anterior midline transperitoneal approach, and L4/5 were punctured by a 33-gauge needle (Hamilton, Reno, NV). A total of 2 ×108 viral genome particles per mouse of Adv-sh-Scramble (5 ×1011 pfu/ml) or Adv-sh-circGPATCH2L (1 ×1011 pfu/ml) were injected into the indicated discs. After the operation, the incision was closed in a layered fashion, and the animals were allowed to move freely around their cages."
M6ACROT05711 .
M6ACROT05712 "BALB/c male nude mice aged 6 weeks were randomly divided into two groups (n = 5 / group). A549 cells with transfection of sh-NC or sh-NEAT1 were injected into mice. After 28 days, the mice were euthanized and the tumor tissues were collected and measured."
M6ACROT05713 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month. "
M6ACROT05714 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month. "
M6ACROT05715 "HeLa cells were stably transfected with LINC00426 overexpression lentivirus. Subsequently, for subcutaneously implanted tumor model, mice were randomly divided into two groups, and cells (1 × 107/100 μl) mixed with the same volume of matrix gel were injected subcutaneously into the right abdomen of mice. One month later the mice were executed and the tumors were stripped and weighed, measured for volume, and used for further analysis. For tumor metastasis assay, mice were randomly grouped, and 5 × 105/100 μl HeLa cells transfected with NC and LINC00426 overexpression lentivirus were intravenously injected into the tail vein of BALB/c nude mice which were sacrificed after 1 month. "
M6ACROT05716 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies"
M6ACROT05717 "The in vivo assay was approved by the Animal Care and Use Committee of Anhui Medical University. And all experimental procedures and animal care were in accordance with the institutional ethics guidelines for animal experiments. The C57BL/6 mice (male, 8-10 weeks old) were housed (six per cage) in a specific and opportunistic pathogen-free facility and maintained on a 12-hlight-dark cycle with casually access to food and water. Detailed descriptions of the cell culture, cardiac fibrosis model, and treatment with lentiviral, were given in the Supplementary methods online."
M6ACROT05718 "The in vivo assay was approved by the Animal Care and Use Committee of Anhui Medical University. And all experimental procedures and animal care were in accordance with the institutional ethics guidelines for animal experiments. The C57BL/6 mice (male, 8-10 weeks old) were housed (six per cage) in a specific and opportunistic pathogen-free facility and maintained on a 12-hlight-dark cycle with casually access to food and water. Detailed descriptions of the cell culture, cardiac fibrosis model, and treatment with lentiviral, were given in the Supplementary methods online."
M6ACROT05719 .
M6ACROT05720 .
M6ACROT05721 .
M6ACROT05722 .
M6ACROT05723 .
M6ACROT05724 .
M6ACROT05725 .
M6ACROT05726 " In the therapeutic study, TIALD stable knockdown or control cells were further infected by lentiviruses (Ubi-MCS-firefly_Luciferase-SV40-neomycin, Genechem, China) and selected by G418 (500 μg/mL). 45 B-NDG mice were divided into 3 groups, including the control group (n = 15), TIALD knockdown group (n = 15) and Alisertib treatment group (n = 15). The modified control cells described above were injected into the mice of control group via tail vein, while the modified TIALD knockdown cells were injected into the mice of TIALD knockdown group and Alisertib treatment group."
M6ACROT05727 .
M6ACROT05728 .
M6ACROT05729 .
M6ACROT05730 .
M6ACROT05731 .
M6ACROT05732 "the GIST-T1 cells were injected into the back flanks of the C57BL/6 female mice (Age six-week-old) at the concentration of 1 × 106 cells diluting in 200 μl PBS buffer solution. The GIST-T1 cells were allowed to grow in mice for a total of 25 days, the mice were sacrificed at day 25 and the tumors were obtained and weighed to evaluate the tumorigenesis abilities of the GIST-T1 cells in vivo. "
M6ACROT05733 "A total of 1 × 107 cotransfected RPMI8226 MM cells, control RPMI8226 MM cells (shNC + LV-NC), ALKBH5-depleted RPMI8226 MM cells (shALKBH5 + LV-NC), or ALKBH5-depleted-SNHG15-overexpressed RPMI8226 MM cells (shALKBH5 + LV-SNHG15), were subcutaneously injected into the right flanks of the 4-wk-old male NOD/SCID mice (n = 6 for each group) to establish a human MM-xenografted model. Tumor growth was monitored every 3 days. The mice were euthanized after 4 wk, and tumors were measured (Tumor volume = π/6 × length × width2) and harvested."
M6ACROT05734 .
M6ACROT05735 "PANC-1 cells were stably transfected with LV-METTL14, LV-NC, sh-METTL14, sh-NC, or sh-LINC00941. A mixture of 2×106 cells and 100 μL PBS was injected into the spleen of every BALB/c nude mouse. After two months of housing in a sterile environment, the mice were sacrificed. Their liver tissues were removed for hematoxylin and eosin (HE) staining."
M6ACROT05736 "PANC-1 cells were stably transfected with LV-METTL14, LV-NC, sh-METTL14, sh-NC, or sh-LINC00941. A mixture of 2×106 cells and 100 μL PBS was injected into the spleen of every BALB/c nude mouse. After two months of housing in a sterile environment, the mice were sacrificed. Their liver tissues were removed for hematoxylin and eosin (HE) staining."
M6ACROT05737 .
M6ACROT05738 .
M6ACROT05739 "Briefly, the mice were intraperitoneally injected with 25 μg OVA mixed with 1 mg aluminum hydroxide gel was intraperitoneally injected into mice once a week for three weeks. Subsequently, nostril challenge with 500 μg OVA was performed once a day for one week. The control mice were exposed to PBS (vehicle). LV-sh-NC or LV-sh-circMIRLET7BHG (1 × 107 TU/mL, GenePharma) were injected into mice via the tail vein two days before the nostril challenge."
M6ACROT05740 "For xenograft growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were inoculated subcutaneously into the axillas of nude mice. The tumor volumes were measured every 3 days. After 21 days, the mice were sacrificed. Simultaneously, the subcutaneous tumors were excised and weighed. For the lung metastatic colonization model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were injected into the tail veins of nude mice. After 5 weeks, the mice were sacrificed, with their lung tissues dissected. All subcutaneous tumors and lung tissues were paraffin embedded and sectioned for subsequent analyses."
M6ACROT05741 "For xenograft growth model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were inoculated subcutaneously into the axillas of nude mice. The tumor volumes were measured every 3 days. After 21 days, the mice were sacrificed. Simultaneously, the subcutaneous tumors were excised and weighed. For the lung metastatic colonization model, 1 × 106 SUNE-1 cells stably expressing scrambled or sh-LINC00839 were injected into the tail veins of nude mice. After 5 weeks, the mice were sacrificed, with their lung tissues dissected. All subcutaneous tumors and lung tissues were paraffin embedded and sectioned for subsequent analyses."
M6ACROT05742 "8-week-old male C57BL/6 mice were randomly divided into three groups: Ctrl+AAV-NC group (n=7), MIA+AAV-NC group (n=7), and MIA+ AAV-IT1 group (n=7). For the induction of OA, mice were given an intra-articular injection of MIA (Sigma-Aldrich, St. Louis, MO, USA) in the knee. Control mice were given normal saline in the same volume. One week later, 20 μL of AAV-HS3ST3B1-IT1 or AAV-NC (GeneChem, Shanghai, China) were injected into the knee joints of mice. Treatments were administered once per week for 3 consecutive weeks. Six weeks later, the mice were euthanized, and the knee joints were collected and store at -80°C."
M6ACROT05743 "The animals were first injected with JAR cells for 7 days, and the mice in AgomiR-935 group, AgomiR-NC group, AntagomiR-935 group, and AntagomiR-NC group were injected via tail vein with miR-935 agomiR (2 nmoL, Ribobio), miR-935 antagomiR (5 nmoL, Ribobio), or same dosage of negative controls (Ribobio) every 3 days for 2 weeks."
M6ACROT05744 "DU145 and PC-3 cells (2 × 106) stably transfected with shMETTL3, shcircABCC4 or sh-NC were subcutaneously injected into the male eight-week-old BALB/c nude mice (16-20 g, Hunan Slac Jingda Laboratory Animal Co., Ltd)."
M6ACROT05745 .
M6ACROT05746 .
M6ACROT05747 .
M6ACROT05748 .
M6ACROT05750 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05751 "A total of 2.0 × 106 SCAPs were mixed with 20 mg HA/tricalcium phosphate (Engineering Research Center for Biomaterials, Sichuan University, Chengdu, China) at 37°C for 2 h, and then, the mixture was subcutaneously transplanted into the back of nude mice (10-week-old female, nu/nu)."
M6ACROT05752 .
M6ACROT05753 .
M6ACROT05754 .
M6ACROT05755 .
M6ACROT05756 .
M6ACROT05757 .
M6ACROT05758 .
M6ACROT05759 .
M6ACROT05760 .
M6ACROT05761 .
M6ACROT05762 "The mice were randomly grouped: (1) shNC + vehicle; (2) shNC + Erastin; (3) shWTAP + Erastin; (4) shcircCMTM3 + Erastin. Briefly, HCC cells with stable silenced WTAP and circCMTM3 (2 × 106 in 0.1 mL PBS) were injected into the left flank of the mice subcutaneously. "
M6ACROT05763 "HCT116 cells stably expressing sh-NC or sh-circ_0124554 were diluted to 2 × 106 cells in 0.2 mL phosphate buffer solution and injected into the mice, followed by 6 Gy irradiation once per day for 5 days. Ten days later, the tumor volume was calculated every 5 days for 5 cycles. Mice were sacrificed after 35 days, and the tumors were dissected for further analysis."
M6ACROT05764 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
M6ACROT05765 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
M6ACROT05766 .
M6ACROT05767 "A549 cells (5 × 106) with LINC00641 stable knockdown or control were suspended in 200 μL of 50% Matrigel (Corning), then injected subcutaneously into the flanks of the nude mice. For the metastatic tumor model, the male BALB/c-nude mice weighing 18-23 g were randomly divided into two groups. A549 cells with LINC00641 stable knockdown or control were injected into the tail veins of the nude mice (2 × 106 cells per mice)."
M6ACROT05768 .
M6ACROT05769 Twelve-week-old wild-type (WT) and IL-10-KO mice were allocated randomly to four groups (n = 5 per group): (1) the WT group; (2) the IL-10 KO + si-control group (administrated saline as a placebo via enema once); (3) the IL-10 KO + si-circPRKAR1B group (administrated 200 μL of 1e11 vg adeno-associated virus (AAV) vector carrying si-circPRKAR1B via enema once); and (4) the IL-10 KO + si-METTL3 group (administrated 200 μL of 1e11 vg AAV vector carrying si-METTL3 via enema once).
M6ACROT05770 "1106 cells/100 L NSCLC cell suspension (oe-NC group, oe-METTL14 + oe-NC group, oe-METTL14 + oe-TXNIP group) were injected into the tail vein. Twenty-four nude mice (8 in each group) were employed. Following the 56th post-injection day, mice were decapitated by decortication. Hematoxylin-eosin (HE) staining was used to identify tumor metastases in the mouse lung tissue "
M6ACROT05771 "Briefly, 4-week nude mice were obtained from Shanghai Slac Laboratory Animal Company. circPUM1-silenced or control SK-N-AS cells were injected into the flanks of mice. 4 weeks later, mice were euthanized to measure tumor weights."
M6ACROT05772 .
M6ACROT05773 "Cells (2 × 104) were seeded onto 12-well plates and cultured in serum-free 1640 medium. Cell spheroids were documented and quantified using an inverted microscope (Olympus, Japan) after two weeks."
M6ACROT05774 "Cells (2 × 104) were seeded onto 12-well plates and cultured in serum-free 1640 medium. Cell spheroids were documented and quantified using an inverted microscope (Olympus, Japan) after two weeks."
M6ACROT05775 .
M6ACROT05776 .
M6ACROT05777 .
M6ACROT05778 .
M6ACROT05779 .
M6ACROT05780 .
M6ACROT05781 .
M6ACROT05782 .
M6ACROT05783 "In brief, HEK293 cells expressing the Flag tag or METTL16-Flag were grown in DMEM and crosslinked at 254 nm (UV-CRAC), or alternatively were grown in DMEM supplemented with 100 μM 4-thiouridine for 6 h prior to crosslinking at 365 nm (PAR-CRAC). Samples were then processed and the obtained sequence reads were mapped to the human genome (GRCh37.p13)."
M6ACROT05785 "To address whether LINC00336 also has a role in lung cancer in vivo, we used a xenograft model. The injection of A549-LINC00336 and SPC-A-1-LINC00336 showed that LINC00336 overexpression significantly increased tumor sizes, volumes, and weights after 1 month of growth compared with A549-Vector and SPC-A-1-Vector cells, whereas the whole-body weight remained unchanged; To address whether LINC00336 has a role in lung cancer in vivo, we used a xenograft model. We injected PC9 cells into nude mice and observed that LINC00336 depletion significantly impaired tumor size, volume, and weight; however, body weights did not change significantly in either group"
M6ACROT05788 "We first confirmed that the expression levels of osteoblast differentiation markers BGLAP, BMP2, Col1a1, and alkaline phosphatase (ALP) were lower in osteoporosis patients and OVX mice than the normal levels in their corresponding control groups"
M6ACROT05790 .
M6ACROT05791 .
M6ACROT05792 .
M6ACROT05793 .
M6ACROT05794 .
M6ACROT05795 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05796 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05797 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05798 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05799 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05800 "We employed a mouse xenograft model. A549 cells stably expressing control or LINC01234-specific short hairpin RNAs (shRNAs) were injected subcutaneously into athymic nude mice, and tumor growth was monitored for 18 days. Indeed, tumors derived from sh-LINC01234 cells were smaller than those derived from sh-NC control cells (Figures 2H and 2I). LINC01234 expression was confirmed to be suppressed in the excised tumors by qPCR analysis (Figure S1G). Compared with the control tumors, sh-LINC01234-derived tumors expressed lower levels of the proliferation marker Ki-67 and contained more apoptotic cells, as assessed by immunohistochemical (IHC) and TUNEL staining, respectively"
M6ACROT05802 .
M6ACROT05803 .
M6ACROT05804 .
M6ACROT05805 "HUVEC were infected with LV-METTL3-shRNA or LV-CT-shRNA particles, as mentioned above. Cells (3×105) were resuspended in 100 μL of medium without FBS and mixed with growth factor reduced matrigel premixed with VEGF (vascular endothelial growth factor)-A165 and bFGF (basic fibroblast growth factor). Samples were injected subcutaneously into the flank region of 10-week-old immunodeficient male mice (Crl:CD1-Foxn1nu, Charles River, United Kingdom; N=8 mice per group)"
M6ACROT05806 "Mice were anesthetized with an intraperitoneal (i.p) injection of pentobarbital sodium (25 mg/kg) and placed on a surgical thermostator. Mice were randomly allocated. Then OIP5-AS1/pcDNA3.1-NC/OIP5-AS1 + si-ADAMTS8 was stably transfected into K1 cells, si-OIP5-AS1/si-NC/si-OIP5-AS1 + ADAMTS8 was stably transfected into TPC-1 cells. Transfected cells were then injected into 4-week-old female BALB/c nude mice (n = 6/per group) at a density of 1 × 106 cells, "
M6ACROT05807 .
M6ACROT05808 .
M6ACROT05809 .
M6ACROT05810 .
M6ACROT05811 .
M6ACROT05812 "The nude mice were divided into three groups: Control group, IGF2BP2-Wt and IGF2BP2-3KR. The animals were free to autoclaved food and water during the experiment. 3×105 cells were subcutaneously injected in the right flanks of the mice. Tumor volume was measured every 5 days and the volume was calculated by the formula: volume (mm3) = length width 2 /2.44 days after injection, mice were sacrificed and tumors were isolated. For survival analysis in orthotopic inoculations, 3×105 cells were stereotactically implanted into the right striatum of the mice. The number of survived nude mice was recorded, and survival analysis was performed using Kaplan Meier survival curve."
M6ACROT05813 "Male Sprague-Dawley rats (6-8 weeks old; weighed from 210 to 230 g) were purchased from HFK Bioscience Co. Ltd. They were housed under standard laboratory conditions. Rats were randomly assigned to four groups (n = 6 per group). The first was the control group, which were treated daily with 0.5 ml of 0.9% saline by intraperitoneal injection for 14 days, and there were three DOX model groups, which were treated three times weekly with 2.5 mg/kg of DOX by intraperitoneal injection for 14 weeks. At day 14, mice in the DOX model groups were infected through an intramyocardial injection of either control shNC or shMettl14 (1 × 109 titer) at three distinct locations in the left ventricular free wall three times a week for 2 weeks, and they were treated daily with 30 mg/kg of ferroptosis inducer erastin (MedChemExpress, USA) through intragastric administration or vehicle control (Saline) for 2 weeks. "
M6ACROT05814 "Male nude mice (age: 4-6 weeks, weight: 22-22 g) provided by the Southern Medical University, were housed in specific pathogen environment at 22-24°C and 50-60% humility under a 12-h light/dark cycle, with ad libitum access to food and water. The nude mice were classified into 3 groups with 18 mice per group: the control group, the sh-NC group, and the sh-METTL3 group. Well-grown ECA109 cells, sh-METTL3-transfected ECA109 cells, sh-NC-transfected ECA109 cells were re-suspended in phosphate buffer saline (PBS) solution and then subcutaneously injected (1 × 107cells/mouse) into the right abdomen (N = 6) or injected into mice via the caudal veins (N = 6). For the subcutaneously injected mice, the tumor volume was evaluated every 7 days according to the following formula: V = ab2/2 (a: the longest diameter of the tumor; b: the shortest diameter of the tumor). After 30 days, the subcutaneously injected mice were euthanatized using an intraperitoneal injection of 1% pentobarbital (200 mg/kg). "
M6ACROT05815 "BALB/c nude mice (4-6 weeks old) were purchased from Hunan SLAC Jingda Experimental Animal Company (Changsha, China). All animal experiments were approved by the Ethical Committee of the First People's Hospital of Chenzhou City, University of South China. Six mice in each group were subcutaneously injected with 5.0 × 106 stable HCT-116 UCA1-KD cells or control cells in 0.2 mL PBS with 50% matrigel as described previously"
M6ACROT05816 "BALB/c nude mice (4-6 weeks old) were purchased from Hunan SLAC Jingda Experimental Animal Company (Changsha, China). All animal experiments were approved by the Ethical Committee of the First People's Hospital of Chenzhou City, University of South China. Six mice in each group were subcutaneously injected with 5.0 × 106 stable HCT-116 UCA1-KD cells or control cells in 0.2 mL PBS with 50% matrigel as described previously"
M6ACROT05817 "BALB/c nude mice (4-6 weeks old) were purchased from Hunan SLAC Jingda Experimental Animal Company (Changsha, China). All animal experiments were approved by the Ethical Committee of the First People's Hospital of Chenzhou City, University of South China. Six mice in each group were subcutaneously injected with 5.0 × 106 stable HCT-116 UCA1-KD cells or control cells in 0.2 mL PBS with 50% matrigel as described previously"
M6ACROT05818 .
M6ACROT05819 .
M6ACROT05820 .
M6ACROT05821 .
M6ACROT05822 .
M6ACROT05823 "BALB/c mice (female) and C57BL/6 mice (male) (8-12 weeks-old, 20-22 g) were obtained from the Shanghai Laboratory Animal Center (Shanghai, China). Pregnancy day 0.5 (E0.5) was determined by measuring the vaginal plug. The m6A inhibitor, 3-deazaadenosine (40 μL at a concentration of 10 mg/kg dissolved in physiological saline; Sigma-Aldrich, St. Louis, MO, USA) was injected into the uterine cavity using a small catheter (Instech Laboratories, Plymouth Meeting, PA, USA) at E6.5, E5.5, and E4.5, respectively, whereas the control group was injected with an equal volume of saline solution. Mice were sacrificed at E10.5, embryo resorption was measured and photographed, and the placentas were collected. The resorbed embryos were small (<20 % of the average size), appeared dark with unclear boundaries between the fetus and the placenta, and showed necrosis."
M6ACROT05824 .
M6ACROT05825 .
M6ACROT05826 .
M6ACROT05827 "A total of six female BALB/c nude mice (4-week-old) were evenly divided to sh-NC group and sh-circ group. Then, 2 × 107 HeLa cells, transfected with either sh-NC or sh-circ, were subcutaneously injected into the mice. The tumor volume was observed and recorded weekly using a vernier caliper. After 4 weeks, the mice were sacrificed, and the tumors were dissected. The tumor from each mouse was imaged, and their weights were measured. This experiment was approved by the Ethics Committee of Wuhan Third Hospital."
M6ACROT05828 .
M6ACROT05829 .
M6ACROT05830 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05831 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05832 DU145 cells (1 × 106 cells) with or without 200-μM propofol treatment were subcutaneously implanted into the right flank of each mouse.
M6ACROT05833 .
M6ACROT05834 .
M6ACROT05836 .
M6ACROT05838 .
M6ACROT05839 .
M6ACROT05840 .
M6ACROT05841 .
M6ACROT05842 .
M6ACROT05843 .
M6ACROT05844 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT05845 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT05846 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT05847 "For cardiomyocyte-specific Kat2a knockdown in vivo, a total of 100 μl (5.0 × 1013 VG/ml) adeno-associated virus 9 (AAV9) carrying shRNA against Kat2a (AAV-shKat2a) was randomly applied per mouse via tail vein injection. AAV9 carrying scramble shRNA (AAV-shNC) was used as control. Cardiac function was assessed four weeks following the AAV injection, and the heart tissues were then isolated."
M6ACROT05848 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT05849 1 × 106 CAL-62 cells were subcutaneously injected into 6-week-old female BALB/c nude mice to establish tumor xenograft models.
M6ACROT05850 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT05851 .
M6ACROT05852 .
M6ACROT05853 .
M6ACROT05854 .
M6ACROT05855 .
M6ACROT05856 .
M6ACROT05857 .
M6ACROT05863 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05864 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05865 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05866 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05867 "Cell suspensions (2 × 106 cells/mL) made with MCF-7/ADR cells stably expressing METTL3 and/or miR-221-3p inhibitor were subcutaneously implanted into each mouse. One week later, xenografted mice were injected with 0.1 mL ADR (25 mg/kg, intraperitoneal injection) twice a week."
M6ACROT05868 .
M6ACROT05869 MGC-803 cells (107 cells suspended in 0.1 ml PBS) were transfected with miR-34a or scramble control then were injected subcutaneously into the left and right dorsal flank of 4-week old Balb/c nude mice respectively. Diameters of tumors were measured and documented each 5 days with a total of 25 days. Tumor volume (mm3) was accessed by measuring the longest and shortest diameter of the tumor and calculating as follows: volume = (shortest diameter) 2 × (longest diameter) × 0.5.
M6ACROT05870 "Eight-week-old C57BL/6 mice were provided by Jinan Pengyue Company and divided into the following groups according to experimental requirements: sham operation group, BLM model group, BLM + si-lnc668/NC group, BLM + si-lnc668 group, overexpressed lnc668/NC group, and overexpressed lnc668 treatment group, with 10 mice in each group. Anesthesia was induced by intraperitoneal injection of 4% chloral hydrate (10 mg/kg). The sham operation group received an equivalent volume of saline as a control."
M6ACROT05871 "Eight-week-old C57BL/6 mice were provided by Jinan Pengyue Company and divided into the following groups according to experimental requirements: sham operation group, BLM model group, BLM + si-lnc668/NC group, BLM + si-lnc668 group, overexpressed lnc668/NC group, and overexpressed lnc668 treatment group, with 10 mice in each group. Anesthesia was induced by intraperitoneal injection of 4% chloral hydrate (10 mg/kg). The sham operation group received an equivalent volume of saline as a control."
M6ACROT05872 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
M6ACROT05873 "The SKOV3 ovarian cancer cell line was transfected with LV2-1 or LV2-NC. Thereafter, BALB/c nude mice (6-week old) were intraperitoneally injected with SKOV3 cells. The mice were killed after 5 weeks, and the number of ascites was determined."
M6ACROT05874 .
M6ACROT05875 "The nude mice were divided into three groups: Control group, IGF2BP2-Wt and IGF2BP2-3KR. The animals were free to autoclaved food and water during the experiment. 3×105 cells were subcutaneously injected in the right flanks of the mice. Tumor volume was measured every 5 days and the volume was calculated by the formula: volume (mm3) = length width 2 /2.44 days after injection, mice were sacrificed and tumors were isolated. For survival analysis in orthotopic inoculations, 3×105 cells were stereotactically implanted into the right striatum of the mice. The number of survived nude mice was recorded, and survival analysis was performed using Kaplan Meier survival curve."
M6ACROT05876 "The nude mice were divided into three groups: Control group, IGF2BP2-Wt and IGF2BP2-3KR. The animals were free to autoclaved food and water during the experiment. 3×105 cells were subcutaneously injected in the right flanks of the mice. Tumor volume was measured every 5 days and the volume was calculated by the formula: volume (mm3) = length width 2 /2.44 days after injection, mice were sacrificed and tumors were isolated. For survival analysis in orthotopic inoculations, 3×105 cells were stereotactically implanted into the right striatum of the mice. The number of survived nude mice was recorded, and survival analysis was performed using Kaplan Meier survival curve."
M6ACROT05877 "For the Matrigel plug assay, HUVECs (1\(\times\)107 cells) incubated with MDA-MB-231-derived exosomes or transfected with the corresponding plasmids were suspended in 100 μl PBS and were mixed with 100 μl the Matrigel basement membrane matrix (Corning), then injected subcutaneously into the nude mice (female, 6-week-old, BALB/c) (n = 6) in the dorsal region."
M6ACROT05878 The tumor xenografts were established by a single intraperitoneal transplantation of 5 × 106 NB4 cells in 0.2 ml of PBS into NOD-SCID mice. The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 μL of PBS was performed to establish intravenous (tail vein) leukemia.
M6ACROT05879 The tumor xenografts were established by a single intraperitoneal transplantation of 5 × 106 NB4 cells in 0.2 ml of PBS into NOD-SCID mice. The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 μL of PBS was performed to establish intravenous (tail vein) leukemia.
M6ACROT05880 The tumor xenografts were established by a single intraperitoneal transplantation of 5 × 106 NB4 cells in 0.2 ml of PBS into NOD-SCID mice. The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 μL of PBS was performed to establish intravenous (tail vein) leukemia.
M6ACROT05881 The tumor xenografts were established by a single intraperitoneal transplantation of 5 × 106 NB4 cells in 0.2 ml of PBS into NOD-SCID mice. The NOD-SCID mice were intravenously (tail vein) implanted with sh-RNA-established NB4 cells. Direct injection of 5 × 106 shRNA-transformed NB4 cells into 150 μL of PBS was performed to establish intravenous (tail vein) leukemia.
M6ACROT05882 .
M6ACROT05883 .
M6ACROT05884 .
M6ACROT05885 .
M6ACROT05886 "The nude mice were grouped randomly without blinding, then 2 × 106 U87TR-sh-NC and U87TR-sh-SOX2OT cells were independently injected subcutaneously into the flanks of nude mice in each group, respectively."
M6ACROT05887 "As previously described, Xenograft in vivo mice assay was performed.13 Transfected MDA-MB-436 or MDA-MB-453 cells with sh-ZEB1-AS1#1 or NC (sh-NC) were subcutaneously injected into mice. Every 3 days, tumor growth was monitored."
M6ACROT05888 .
M6ACROT05889 "For xenograft experiments, six to eight-week-old BALB/c-nu/nu mice were used. Huh7 cells (2 × 106) that had been stably transfected with overexpression, interfering or control fragment were suspended in 200 μl serum-free DMEM and implanted subcutaneously into the nude mice. For the lateral tail vein metastasis model, 3 × 106 treated Huh7 cells were suspended in 200 μl serum-free DMEM and injected into the nude mice. "
M6ACROT05890 "To address whether LINC00336 also has a role in lung cancer in vivo, we used a xenograft model. The injection of A549-LINC00336 and SPC-A-1-LINC00336 showed that LINC00336 overexpression significantly increased tumor sizes, volumes, and weights after 1 month of growth compared with A549-Vector and SPC-A-1-Vector cells, whereas the whole-body weight remained unchanged; To address whether LINC00336 has a role in lung cancer in vivo, we used a xenograft model. We injected PC9 cells into nude mice and observed that LINC00336 depletion significantly impaired tumor size, volume, and weight; however, body weights did not change significantly in either group"
M6ACROT05891 "Briefly, control or ""THOR-KO"" HONE-1 cells (1 × 107 cells per mouse, in 100 μL DMEM and 100 μL Matrigel, no serum) were inoculated (via s.c. injection) to the female severe combined immuno-deficient (SCID) mice (4-5 week old, 17-18 g weight)."
M6ACROT05892 "Briefly, control or ""THOR-KO"" HONE-1 cells (1 × 107 cells per mouse, in 100 μL DMEM and 100 μL Matrigel, no serum) were inoculated (via s.c. injection) to the female severe combined immuno-deficient (SCID) mice (4-5 week old, 17-18 g weight)."
M6ACROT05893 "Briefly, control or ""THOR-KO"" HONE-1 cells (1 × 107 cells per mouse, in 100 μL DMEM and 100 μL Matrigel, no serum) were inoculated (via s.c. injection) to the female severe combined immuno-deficient (SCID) mice (4-5 week old, 17-18 g weight)."
M6ACROT05894 "Briefly, 4-week nude mice were obtained from Shanghai Slac Laboratory Animal Company. circPUM1-silenced or control SK-N-AS cells were injected into the flanks of mice. 4 weeks later, mice were euthanized to measure tumor weights."
M6ACROT05895 .
M6ACROT05896 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
M6ACROT05897 "SKOV3 cells were treated with sh-NC or sh-circASXL1. 100 μL of PBS containing 1 × 106 cells were implanted into mice. Tumor volume was measured every 5 days. After 30 days, mice were euthanized. The tumor was excised and weighed."
M6ACROT05898 "HCT116 cells stably expressing sh-NC or sh-circ_0124554 were diluted to 2 × 106 cells in 0.2 mL phosphate buffer solution and injected into the mice, followed by 6 Gy irradiation once per day for 5 days. Ten days later, the tumor volume was calculated every 5 days for 5 cycles. Mice were sacrificed after 35 days, and the tumors were dissected for further analysis."
M6ACROT05899 "The mice were randomly grouped: (1) shNC + vehicle; (2) shNC + Erastin; (3) shWTAP + Erastin; (4) shcircCMTM3 + Erastin. Briefly, HCC cells with stable silenced WTAP and circCMTM3 (2 × 106 in 0.1 mL PBS) were injected into the left flank of the mice subcutaneously. "
M6ACROT05900 .
M6ACROT05901 .
M6ACROT05902 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05903 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05904 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05905 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05906 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05907 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05908 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05909 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05910 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05911 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05912 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05913 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05914 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05915 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05916 "Before subcutaneous injection, A549 cells were seeded into T-75 flask (Corning, USA) and transfected with vectors (control vector, LINC01006 overexpression and METTL3 overexpression, 10 μg respectively) or siRNA pools (LINC01006 and METTL3, 100 nM respectively) by Lipofectamine 2000 (Invitrogen, USA) based on the provided protocol when cell confluence reached 80%. After 48 h, counted 1x107 transfected A549 cells and suspended in 100 ul PBS. The suspended cells were injected into the same side armpit of mice."
M6ACROT05917 "A total of 5×106 Caski cells were subcutaneously injected into the left inguen of mice. When tumors were ~50 mm3±10% (day16). The mice were randomly divided into 4 groups and received piRNA-17458 mimic or piRNA-17458 inhibitor by intratumor injection for every 4 days for a total of five injections. On the 36th day, the tumor weight and lymph node metastasis of the nude mice were assessed after mice were euthanized."
M6ACROT05918 "DU145 and PC-3 cells (2 × 106) stably transfected with shMETTL3, shcircABCC4 or sh-NC were subcutaneously injected into the male eight-week-old BALB/c nude mice (16-20 g, Hunan Slac Jingda Laboratory Animal Co., Ltd)."
M6ACROT05919 "Briefly, the mice were intraperitoneally injected with 25 μg OVA mixed with 1 mg aluminum hydroxide gel was intraperitoneally injected into mice once a week for three weeks. Subsequently, nostril challenge with 500 μg OVA was performed once a day for one week. The control mice were exposed to PBS (vehicle). LV-sh-NC or LV-sh-circMIRLET7BHG (1 × 107 TU/mL, GenePharma) were injected into mice via the tail vein two days before the nostril challenge."
M6ACROT05920 "To create a xenograft model, 1 × 107 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were subcutaneously injected into the nude mice. Tumor sizes were calculated by measuring the length and width (V = length × width2/2). Mice were euthanized four weeks later, and tumors were weighed. For in vivo liver metastasis assay, 1 × 106 SW620 cells stably transfected shMETTL3 and shcircUHRF2 were injected into the distal tip of the spleen of mice according to previous studies"
M6ACROT05921 .
M6ACROT05922 Four-week-old male BALB/c nude mice were used for animal experiments. UM-UC3 cells (2 × 106 cells per mouse) stably overexpressing miR-5581-3p and NC were injected into the mice to establish the subcutaneous implantation model.
M6ACROT05923 "C57BL/6 mice (6-8 weeks old, female) were used to create tumor models by subcutaneous injection of GC cells. When tumors reached ~ 100 mm3, mice were injected with PLGA-PEG(si-circRHBDD1) NPs or PLGA-PEG(siRNA control) NPs (200 mg/kg) via tail vein. "
M6ACROT05924 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05925 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05926 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05927 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05928 "Subcutaneous tumorigenesis was induced in mice by subcutaneous transplantation of 1 × 106 HL-60 cells. To stably overexpress GNAS-AS1, lentivirus-packaged OE-GNAS-AS1 (1 × 108 plaque-forming units). When the tumor volume reached 150-200 mm3, mice in the control group were orally treated with 1% DMSO (containing 0.2% carboxymethylcellulose and 0.1% Tween 80). Mice in the Chidamide, Chidamide + OE-NC, and Chidamide + OE-GNAS-AS1 groups were orally treated with Chidamide (25 mg/kg body weight, formulated with 1% DMSO containing 0.2% carboxymethylcellulose and 0.1% Tween 80). All treatments were repeated three times a week and for two weeks. Tumor volume was recorded every 5 days."
M6ACROT05929 "The growth of CC cells in vivo was examined by subcutaneous injection of LoVo cells or Caco-2 cells into NSG mice, while the metastatic ability of cells in vivo by intracardiac injection into mice."
M6ACROT05930 "For the unilateral ureteral obstruction (UUO) model, male C57BL/6J mice at 8 weeks of age (20-22 g body weight) were first anaesthetized with pentobarbital sodium (50 mg/kg) via intraperitoneal injection. Then, the left ureter was ligated using 3-0 silk and a left lateral incision."
M6ACROT05931 "The nude mice were randomly grouped in the sh-NC + oe-NC group, the sh-UCA1 + oe-NC group, and the sh-UCA1 + oe-SOX12 group (eight mice in each group). Next, 20 μL transfected cells (1 × 107 cells/mL) were inoculated subcutaneously into the axilla of nude mice."
M6ACROT05932 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05933 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05934 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05935 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05936 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05937 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05938 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05939 "cells were collected from the CD45.2 Ythdf2fl/flMx1-Cre mice, enriched for lin-population, and transduced with retroviral MA9 alone or retroviral MA9 + MSCV-pre-miR-126/MSCV-pre-miR-Control by spinoculation as described before. Clonal cells from CFA assays were washed and suspended together with healthy mouse BM cells (0.5 million CFA cells + 1 million healthy BM per mouse) with ice-cold PBS, and subsequently injected via the tail vein into lethally (900 rads) irradiated 8 to 10-week-old B6.SJL (CD45.1) recipient mice. Ten days after the injection, knockout was induced by i.p. delivering of poly I:C (428750 R&D Systems) at 300 μg each time per mice every other day for five times. Mice were observed regularly after cell injection and samples were collected at the end point."
M6ACROT05940 .
M6ACROT05941 "For subcutaneous xenograft models, 0.1 mL of cell suspension containing 106 cells were injected subcutaneously into the right flank of mice (n = 6 for each group)."
M6ACROT05942 .
M6ACROT05943 .
M6ACROT05944 .
M6ACROT05945 .
M6ACROT05946 .
M6ACROT05947 .
M6ACROT05948 .
M6ACROT05949 .
M6ACROT05950 "Nude (nu/nu) mice (4-5 weeks old) were purchased from Harlan Laboratories (Indianapolis, IN, USA). All animal studies were conducted in accordance with NIH animal use guidelines and a protocol approved by the UMMC Animal Care Committee. MIA PaCa-2 cells with LINC00901 overexpression or vector control (pCDH); LINC00901 KO or vector control (LCV2-m) at the exponential stage were harvested and mixed with 50% matrigel, and then injected into mice (2.5 million cells/spot) s.c. as described previously.21 Tumor growth was measured every 4 days, starting 2 weeks after injection of tumor cells."
M6ACROT05951 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05952 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05953 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05954 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05955 "Sexually mature ICR mice, aged at least 10 weeks, were paired as one female and one male per cage to obtain pregnancies. The time of the vaginal plug sighting was denoted as day 0.5 of the pregnancy. The mice were divided into an FTO protein group and control group, with 10 pairs of mice in each group. Starting on day 0.5 of the pregnancy, mice in the FTO protein group were intraperitoneally injected daily with FTO protein (3.5 ng/kg), whereas those in the control group had daily intraperitoneal injections of equal volumes of saline. Female mice were euthanised on day 13.5 of the pregnancy (day 12.5-14.5), and the total numbers of normal and resorbed embryos were determined, along with the rate of embryo resorption."
M6ACROT05956 .
M6ACROT05957 .
M6ACROT05958 "Ten mice were included in each group, and lentivirus-transduced UM-UC-3 cells (3 × 106 cells) that stably expressed firefly luciferase were inoculated into the mice's footpads."
M6ACROT05959 "Ten mice were included in each group, and lentivirus-transduced UM-UC-3 cells (3 × 106 cells) that stably expressed firefly luciferase were inoculated into the mice's footpads."
M6ACROT05960 "Ten mice were included in each group, and lentivirus-transduced UM-UC-3 cells (3 × 106 cells) that stably expressed firefly luciferase were inoculated into the mice's footpads."
M6ACROT05961 "The in vivo tumour growth assays were operated mainly as previously described.18 Briefly, 5 8 × 106 PCDH (empty vector) or 942-OE (transduced with overexpression plasmid) or plvx(empty vector)+shNC, plvx-942-OE+shNC, plvx+shDN3a, 942-OE+shDN3a SGC79001 stably infected cell lines were subcutaneously injected into the dorsal flanks of each mouse (n = 8 or 5). After about 1 week, the xenograft mice were divided into two or four groups stochastically. "
M6ACROT05962 "The in vivo tumour growth assays were operated mainly as previously described.18 Briefly, 5 8 × 106 PCDH (empty vector) or 942-OE (transduced with overexpression plasmid) or plvx(empty vector)+shNC, plvx-942-OE+shNC, plvx+shDN3a, 942-OE+shDN3a SGC79001 stably infected cell lines were subcutaneously injected into the dorsal flanks of each mouse (n = 8 or 5). After about 1 week, the xenograft mice were divided into two or four groups stochastically. "
M6ACROT05963 "Mice were anesthetized with an intraperitoneal (i.p) injection of pentobarbital sodium (25 mg/kg) and placed on a surgical thermostator. Mice were randomly allocated. Then OIP5-AS1/pcDNA3.1-NC/OIP5-AS1 + si-ADAMTS8 was stably transfected into K1 cells, si-OIP5-AS1/si-NC/si-OIP5-AS1 + ADAMTS8 was stably transfected into TPC-1 cells. Transfected cells were then injected into 4-week-old female BALB/c nude mice (n = 6/per group) at a density of 1 × 106 cells, "
M6ACROT05964 .
M6ACROT05965 .
M6ACROT05966 .
M6ACROT05967 .
M6ACROT05968 .
M6ACROT05969 Each mouse was injected with 5 × 106 MIR4435-2HG overexpressed Huh7 cells on the left armpit and control on the right armpit (six mice per group).
M6ACROT05970 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05971 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05972 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05973 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05974 "After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice."
M6ACROT05975 .
M6ACROT05976 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05977 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05978 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05979 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05980 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05981 "The experimental mice were housed in an SPF-grade animal laboratory at a temperature of approximately 25 ° C, a humidity range of 50%, and an average daylight duration of 12 h. BALB/C female mice at 5-6 weeks of age were taken and prepared for tumor-bearing. After the mice were sacrificed, their tissues were collected, weighed and immediately snap-frozen in liquid nitrogen."
M6ACROT05982 .
M6ACROT05983 .
M6ACROT05984 .
M6ACROT05985 .
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M6ACROT05993 "MDA-MB-231 cells (1×106 cells/mouse) were mixed with an equivalent number of NFs/Ctrl, CAFs/shNC, CAFs/sh lncSNHG5, CAFs/shZNF281, CAFs/sh lncSNHG5/ZNF281, CAFs/RS102895, CAFs/Maraviroc or CAFs/Cenicriviroc in 200 l PBS:Matrigel at a ratio of 1:1. "
M6ACROT05994 "MDA-MB-231 cells (1×106 cells/mouse) were mixed with an equivalent number of NFs/Ctrl, CAFs/shNC, CAFs/sh lncSNHG5, CAFs/shZNF281, CAFs/sh lncSNHG5/ZNF281, CAFs/RS102895, CAFs/Maraviroc or CAFs/Cenicriviroc in 200 l PBS:Matrigel at a ratio of 1:1. "
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M6ACROT06021 "After randomly assignment and anesthetization, nude mice were injected with 5 × 106 cells suspended in 100 uL PBS into the tail vein (n = 5 per group). "
M6ACROT06022 "Five-week-old male BALB/c nude mice were used for tumor growth studies in vivo. Briefly, AGS cells were subcutaneously injected into the dorsal side of mice blindly and randomly (n = 5 per group)."
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M6ACROT06028 "IMQ-induced psoriatic model was constructed by applying 10 mg per ear 5% IMQ for 8 consecutive days, and 6 ug macrophage-specific control or hsa_circ_0004287 plasmid was topically applied every 2 days (5 mice per group per experiment)."
M6ACROT06029 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
M6ACROT06030 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
M6ACROT06031 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
M6ACROT06032 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
M6ACROT06033 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
M6ACROT06034 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
M6ACROT06035 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
M6ACROT06036 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
M6ACROT06037 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
M6ACROT06038 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21380)."
M6ACROT06039 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21381)."
M6ACROT06040 "For the generation of PDX mouse models, fresh LUSC specimens (2-3 mm3), which were from Shanghai Chest Hospital, were implanted into 4- to 6-week-old athymic nude mice (Jiesijie, Shanghai, China). After successful tumor growth was confirmed, the tumor tissues were passaged and implanted into the next generation of mice. The third to fifth generations of PDX-bearing mice were used for drug administration. When tumors reached approximately 200 mm3, mice were injected daily with DMSO (no. ST038, Beyotime Biotechnology, Shanghai, China) with or without MIT (no. S2485, 5 mg/kg, Selleck, Houston, TX) or HYD (no. S1896, 20 mg/kg, Selleck) (n = 5 mice/group). Tumor growth was monitored, and sizes were calculated by 0.5 × L × W2 (L indicating length and W indicating width). The mice were euthanized at day 28 after implantation. All animal experiments were approved by the institutional ethics committee of Shanghai Chest Hospital (approval number KS(Y)21382)."
M6ACROT06041 "A total of 5 × 106 PATU-8988 or organoids derived from PDAC patients (PDO) were orthotopically or subcutaneously injected into per BALB/c nude mice. For the mouse model of CSF1Ri application, 5 × 106 PancO2 cells were orthotopically or subcutaneously injected into per C57BL/6 nude mice."
M6ACROT06042 "A total of 5 × 106 PATU-8988 or organoids derived from PDAC patients (PDO) were orthotopically or subcutaneously injected into per BALB/c nude mice. For the mouse model of CSF1Ri application, 5 × 106 PancO2 cells were orthotopically or subcutaneously injected into per C57BL/6 nude mice."
M6ACROT06043 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT06044 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.
M6ACROT06045 A total of 106 HCT116 cells suspended in 100 L of PBS were subcutaneously injected into the axilla of each nude mouse.